3t8j - Revision history

OCA at 13:20, 14 March 2024

OCA — Thu, 14 Mar 2024 13:20:49 GMT

←Older revision		Revision as of 13:20, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t8j' size='340' side='right'caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3t8j' size='340' side='right'caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Sacs2 Sacs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T8J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[2mas\|2mas]]~~, [[~~1q8f~~\|~~1q8f~~]]~~, [[3g5i\|3g5i]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>iunH-1, SSO0505 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~273057 SACS2])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [https://pdbe.org/3t8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [https://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [https://pdbe.org/3t8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [https://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q97ZS5_SACS2 Q97ZS5_SACS2]
-	The purine and pyrimidine-specific nucleoside hydrolases (NHs) from the archaeon Sulfolobus solfataricus participate in the fundamental pathway of nucleotide catabolism, and concur in maintaining adequate levels of free nitrogenous bases for cellular function. The two highly homologous isozymes display distinct specificities towards nucleoside substrates, and both lack the amino acids employed for activation of the leaving group in the hydrolytic reaction by the so far characterized NHs. We determined the high-resolution crystal structures of the purine and pyrimidine-specific NHs from S. solfataricus to reveal that both enzymes belong to the NH structural homology group I, despite the different substrate specificities. A Na+ ion is bound at the active site of the pyrimidine-specific NH instead of the prototypical Ca2+, delineating a role of the metals in the catalytic mechanism of NHs in the substrate binding rather than nucleophile activation. A conserved His residue, that regulates product release in other homologous NHs, provides crucial interactions for leaving group activation in the archaeal isozymes. Modeling of the enzyme-substrate interactions suggests that steric exclusion and catalytic selection underlie the orthogonal base specificity of the two isozymes.	+
-		+
-	New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.,Minici C, Cacciapuoti G, De Leo E, Porcelli M, Degano M Biochemistry. 2012 May 2. PMID:~~22551416<ref>PMID:22551416<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3t8j" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Purine nucleosidase~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: ~~Sacs2~~]]	+	[[Category: Cacciapuoti G]]
-	[[Category: ~~Cacciapuoti, G~~]]	+	[[Category: De Leo E]]
-	[[Category: Degano, M]]	+	[[Category: Degano M]]
-	~~[[Category: Leo, E De~~]]	+	[[Category: Minici C]]
-	[[Category: Minici, C]]	+	[[Category: Porcelli M]]
-	[[Category: Porcelli, M]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: N-glycosidase]]~~	+
-	~~[[Category: Nh-fold]]~~	+
-	~~[[Category: Nucleoside hydrolase]]~~	+
-	~~[[Category: Nucleotide metabolism]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Thermostable protein~~]]	+

OCA at 16:47, 6 July 2022

OCA — Wed, 06 Jul 2022 16:47:32 GMT

←Older revision		Revision as of 16:47, 6 July 2022
Line 1:		Line 1:

	==Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase==		==Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase==
-	<StructureSection load='3t8j' size='340' side='right' caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3t8j' size='340' side='right'caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sulso Sulso~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 ~~SULSO~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [~~http~~://pdbe.org/3t8j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [https://pdbe.org/3t8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [https://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Purine nucleosidase]]		[[Category: Purine nucleosidase]]
-	[[Category: ~~Sulso~~]]	+	[[Category: Sacs2]]
	[[Category: Cacciapuoti, G]]		[[Category: Cacciapuoti, G]]
	[[Category: Degano, M]]		[[Category: Degano, M]]

OCA at 21:08, 11 August 2016

OCA — Thu, 11 Aug 2016 21:08:37 GMT

←Older revision		Revision as of 21:08, 11 August 2016
Line 1:		Line 1:
		+
	==Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase==		==Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase==
	<StructureSection load='3t8j' size='340' side='right' caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3t8j' size='340' side='right' caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T8J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~2287 Sulfolobus solfataricus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [http://www.ebi.ac.uk/pdbsum/3t8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [http://pdbe.org/3t8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [http://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t8j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Purine nucleosidase]]		[[Category: Purine nucleosidase]]
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Sulso]]
	[[Category: Cacciapuoti, G]]		[[Category: Cacciapuoti, G]]
	[[Category: Degano, M]]		[[Category: Degano, M]]

OCA at 12:59, 5 January 2015

OCA — Mon, 05 Jan 2015 12:59:01 GMT

←Older revision		Revision as of 12:59, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T8J FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T8J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [http://www.ebi.ac.uk/pdbsum/3t8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [http://www.ebi.ac.uk/pdbsum/3t8j PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	[[Category: Purine nucleosidase]]		[[Category: Purine nucleosidase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Cacciapuoti, G.]]	+	[[Category: Cacciapuoti, G]]
-	[[Category: Degano, M.]]	+	[[Category: Degano, M]]
-	[[Category: Leo, E De.]]	+	[[Category: Leo, E De]]
-	[[Category: Minici, C.]]	+	[[Category: Minici, C]]
-	[[Category: Porcelli, M.]]	+	[[Category: Porcelli, M]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: N-glycosidase]]		[[Category: N-glycosidase]]

OCA at 07:49, 9 July 2014

OCA — Wed, 09 Jul 2014 07:49:16 GMT

←Older revision		Revision as of 07:49, 9 July 2014
Line 1:		Line 1:
-	[[~~Image:~~3t8j.~~png~~\|~~left~~\|~~200px~~]]	+	==Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase==
		+	<StructureSection load='3t8j' size='340' side='right' caption='[[3t8j]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T8J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2mas\|2mas]], [[1q8f\|1q8f]], [[3g5i\|3g5i]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [http://www.ebi.ac.uk/pdbsum/3t8j PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The purine and pyrimidine-specific nucleoside hydrolases (NHs) from the archaeon Sulfolobus solfataricus participate in the fundamental pathway of nucleotide catabolism, and concur in maintaining adequate levels of free nitrogenous bases for cellular function. The two highly homologous isozymes display distinct specificities towards nucleoside substrates, and both lack the amino acids employed for activation of the leaving group in the hydrolytic reaction by the so far characterized NHs. We determined the high-resolution crystal structures of the purine and pyrimidine-specific NHs from S. solfataricus to reveal that both enzymes belong to the NH structural homology group I, despite the different substrate specificities. A Na+ ion is bound at the active site of the pyrimidine-specific NH instead of the prototypical Ca2+, delineating a role of the metals in the catalytic mechanism of NHs in the substrate binding rather than nucleophile activation. A conserved His residue, that regulates product release in other homologous NHs, provides crucial interactions for leaving group activation in the archaeal isozymes. Modeling of the enzyme-substrate interactions suggests that steric exclusion and catalytic selection underlie the orthogonal base specificity of the two isozymes.

-	~~{{STRUCTURE_3t8j\| PDB=3t8j \| SCENE= }}~~	+	New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.,Minici C, Cacciapuoti G, De Leo E, Porcelli M, Degano M Biochemistry. 2012 May 2. PMID:22551416<ref>PMID:22551416</ref>

-	~~===Structural analysis~~ of ~~thermostable~~ S. ~~solfataricus pyrimidine-specific nucleoside hydrolase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22551416}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3t8j]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022551416<~~/~~ref~~><~~ref group="xtra">PMID:018355316</ref><references group="xtra"~~/>	+
	[[Category: Purine nucleosidase]]		[[Category: Purine nucleosidase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]

OCA at 06:33, 3 October 2012

OCA — Wed, 03 Oct 2012 06:33:18 GMT

←Older revision		Revision as of 06:33, 3 October 2012
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-	[[Image:3t8j.~~jpg~~\|left\|200px]]	+	[[Image:3t8j.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3t8j\| PDB=3t8j \| SCENE= }}		{{STRUCTURE_3t8j\| PDB=3t8j \| SCENE= }}

	===Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase===		===Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22551416}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_22551416}}		{{ABSTRACT_PUBMED_22551416}}

OCA at 09:14, 16 May 2012

OCA — Wed, 16 May 2012 09:14:20 GMT

←Older revision		Revision as of 09:14, 16 May 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3t8j.jpg\|left\|200px]]

-	The ~~entry 3t8j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t8j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t8j\| PDB=3t8j \| SCENE= }}

-	~~Authors: Minici, C., Cacciapuoti, G., De Leo, E., Porcelli, M., Degano, M~~.	+	===Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase===

-	~~Description~~: ~~Structural analysis of thermostable S~~. solfataricus ~~pyrimidine~~-~~specific nucleoside~~ hydrolase	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22551416}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22551416 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22551416}}
		+
		+	==About this Structure==
		+	[[3t8j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022551416</ref><ref group="xtra">PMID:018355316</ref><references group="xtra"/>
		+	[[Category: Purine nucleosidase]]
		+	[[Category: Sulfolobus solfataricus]]
		+	[[Category: Cacciapuoti, G.]]
		+	[[Category: Degano, M.]]
		+	[[Category: Leo, E De.]]
		+	[[Category: Minici, C.]]
		+	[[Category: Porcelli, M.]]
		+	[[Category: Hydrolase]]
		+	[[Category: N-glycosidase]]
		+	[[Category: Nh-fold]]
		+	[[Category: Nucleoside hydrolase]]
		+	[[Category: Nucleotide metabolism]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Thermostable protein]]

OCA: Protected "3t8j" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:37:09 GMT

Protected "3t8j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 10 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t8j is ON HOLD until Paper Publication Authors: Minici, C., Cacciapuoti, G., De Leo, E., Porcelli, M., Degano, M. Description: Structural analysis...

OCA — Wed, 10 Aug 2011 05:37:08 GMT

New page: '''Unreleased structure''' The entry 3t8j is ON HOLD until Paper Publication Authors: Minici, C., Cacciapuoti, G., De Leo, E., Porcelli, M., Degano, M. Description: Structural analysis...

New page

'''Unreleased structure'''

The entry 3t8j is ON HOLD until Paper Publication

Authors: Minici, C., Cacciapuoti, G., De Leo, E., Porcelli, M., Degano, M.

Description: Structural analysis of thermostable S. solfataricus pyrimidine-specific nucleoside hydrolase