3t94 - Revision history

OCA at 13:21, 14 March 2024

OCA — Thu, 14 Mar 2024 13:21:22 GMT

←Older revision		Revision as of 13:21, 14 March 2024
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	<StructureSection load='3t94' size='340' side='right'caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='3t94' size='340' side='right'caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T94 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T94 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MTA:5~~-~~DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.452Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2a8y\|2a8y]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [https://pdbe.org/3t94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [https://www.ebi.ac.uk/pdbsum/3t94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t94 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [https://pdbe.org/3t94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [https://www.ebi.ac.uk/pdbsum/3t94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t94 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~MTAP_SULSO MTAP_SULSO]~~] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.~~<ref>PMID:15819883</ref>~~	+	[https://www.uniprot.org/uniprot/MTAP_SACS2 MTAP_SACS2] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.[HAMAP-Rule:MF_01963]<ref>PMID:15819883</ref>
-	~~<div style="background~~-~~color~~:~~#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine (MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for MTA. SsMTAP I forms a donut-shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in space group P1 (PDB entry 2a8y) and showed R32 pseudosymmetry. Post-analysis using phenix.xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space-group assignment are discussed.	+
-		+
-	A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.,Zhang Y, Zwart PH, Ealick SE Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):249-52. Epub 2012 Feb 7. PMID:22349226<ref>PMID:~~22349226~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t94" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~S-methyl-5'-thioadenosine phosphorylase~~]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: Ealick~~, S E~~]]	+	[[Category: Ealick SE]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang Y]]
-	~~[[Category: Alpha/beta]]~~	+
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Nucleoside phosphorylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:48, 6 July 2022

OCA — Wed, 06 Jul 2022 16:48:11 GMT

←Older revision		Revision as of 16:48, 6 July 2022
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	==Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate==		==Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate==
-	<StructureSection load='3t94' size='340' side='right' caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='3t94' size='340' side='right'caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T94 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T94 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a8y\|2a8y]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [~~http~~://pdbe.org/3t94 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t94 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t94 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [https://pdbe.org/3t94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [https://www.ebi.ac.uk/pdbsum/3t94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t94 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MTAP_SULSO MTAP_SULSO]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.<ref>PMID:15819883</ref>	+	[[https://www.uniprot.org/uniprot/MTAP_SULSO MTAP_SULSO]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.<ref>PMID:15819883</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[5'-deoxy-5'-methylthioadenosine phosphorylase\|5'-deoxy-5'-methylthioadenosine phosphorylase]]	+	*[[5'-deoxy-5'-methylthioadenosine phosphorylase 3D structures\|5'-deoxy-5'-methylthioadenosine phosphorylase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 35091]]		[[Category: Atcc 35091]]
		+	[[Category: Large Structures]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 08:44, 4 August 2016

OCA — Thu, 04 Aug 2016 08:44:14 GMT

←Older revision		Revision as of 08:44, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate==		==Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate==
	<StructureSection load='3t94' size='340' side='right' caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='3t94' size='340' side='right' caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T94 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T94 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [http://www.ebi.ac.uk/pdbsum/3t94 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [http://pdbe.org/3t94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [http://www.ebi.ac.uk/pdbsum/3t94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t94 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q97W94_SULSO Q97W94_SULSO~~]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.<ref>PMID:15819883</ref>	+	[[http://www.uniprot.org/uniprot/MTAP_SULSO MTAP_SULSO]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.<ref>PMID:15819883</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t94" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 35091]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
-	[[Category: Sulfolobus solfataricus]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]
	[[Category: Zhang, Y]]		[[Category: Zhang, Y]]

OCA at 07:27, 25 December 2014

OCA — Thu, 25 Dec 2014 07:27:53 GMT

←Older revision		Revision as of 07:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T94 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T94 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [http://www.ebi.ac.uk/pdbsum/3t94 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [http://www.ebi.ac.uk/pdbsum/3t94 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q97W94_SULSO Q97W94_SULSO]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.<ref>PMID:15819883</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.,Zhang Y, Zwart PH, Ealick SE Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):249-52. Epub 2012 Feb 7. PMID:22349226<ref>PMID:22349226</ref>		A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.,Zhang Y, Zwart PH, Ealick SE Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):249-52. Epub 2012 Feb 7. PMID:22349226<ref>PMID:22349226</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[5'-deoxy-5'-methylthioadenosine phosphorylase\|5'-deoxy-5'-methylthioadenosine phosphorylase]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 27:
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Alpha/beta]]		[[Category: Alpha/beta]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Nucleoside phosphorylase]]		[[Category: Nucleoside phosphorylase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:57, 5 June 2014

OCA — Thu, 05 Jun 2014 05:57:07 GMT

←Older revision		Revision as of 05:57, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t94.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate==
		+	<StructureSection load='3t94' size='340' side='right' caption='[[3t94]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T94 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a8y\|2a8y]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t94 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t94 RCSB], [http://www.ebi.ac.uk/pdbsum/3t94 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine (MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for MTA. SsMTAP I forms a donut-shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in space group P1 (PDB entry 2a8y) and showed R32 pseudosymmetry. Post-analysis using phenix.xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space-group assignment are discussed.

-	<!--	+	A corrected space group for Sulfolobus sulfataricus 5'-deoxy-5'-methylthioadenosine phosphorylase II.,Zhang Y, Zwart PH, Ealick SE Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):249-52. Epub 2012 Feb 7. PMID:22349226<ref>PMID:22349226</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t94"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3t94\| PDB=3t94 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22349226}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22349226 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22349226}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t94]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022349226<~~/~~ref~~><~~ref group="xtra">PMID:016414070</ref><references group="xtra"~~/>	+
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]

OCA at 06:43, 28 March 2012

OCA — Wed, 28 Mar 2012 06:43:18 GMT

←Older revision		Revision as of 06:43, 28 March 2012
Line 13:		Line 13:

	<!--		<!--
-	The line below this paragraph, {{~~ABSTRACT_PUBMED_16414070~~}}, adds the Publication Abstract to the page	+	The line below this paragraph, {{ABSTRACT_PUBMED_22349226}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where ~~16414070~~ is the PubMed ID number.	+	(as it appears on PubMed at http://www.pubmed.gov), where 22349226 is the PubMed ID number.
	-->		-->
-	{{~~ABSTRACT_PUBMED_16414070~~}}	+	{{ABSTRACT_PUBMED_22349226}}

	==About this Structure==		==About this Structure==
Line 22:		Line 22:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:016414070</ref><references group="xtra"/>	+	<ref group="xtra">PMID:022349226</ref><ref group="xtra">PMID:016414070</ref><references group="xtra"/>
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]

OCA at 07:42, 29 February 2012

OCA — Wed, 29 Feb 2012 07:42:49 GMT

←Older revision		Revision as of 07:42, 29 February 2012
Line 1:		Line 1:
-	[[Image:3t94.~~jpg~~\|left\|200px]]	+	[[Image:3t94.png\|left\|200px]]

	<!--		<!--

OCA at 07:36, 24 August 2011

OCA — Wed, 24 Aug 2011 07:36:21 GMT

←Older revision		Revision as of 07:36, 24 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t94.jpg\|left\|200px]]

-	The ~~entry 3t94~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t94", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t94\| PDB=3t94 \| SCENE= }}

-	~~Authors: Zhang, Y., Ealick, S.E.~~	+	===Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate===

-	~~Description: Crystal structure of 5'~~-~~deoxy~~-~~5'-methylthioadenosine phosphorylase~~ (~~MTAP~~) ~~II complexed~~ with 5'-~~deoxy~~-5'-~~methylthioadenosine and sulfate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_16414070}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16414070 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16414070}}
		+
		+	==About this Structure==
		+	[[3t94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T94 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:016414070</ref><references group="xtra"/>
		+	[[Category: S-methyl-5'-thioadenosine phosphorylase]]
		+	[[Category: Sulfolobus solfataricus]]
		+	[[Category: Ealick, S E.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Alpha/beta]]
		+	[[Category: Beta barrel]]
		+	[[Category: Nucleoside phosphorylase]]
		+	[[Category: Transferase]]

OCA: Protected "3t94" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:37:48 GMT

Protected "3t94" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 10 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t94 is ON HOLD Authors: Zhang, Y., Ealick, S.E. Description: Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexe...

OCA — Wed, 10 Aug 2011 05:37:47 GMT

New page: '''Unreleased structure''' The entry 3t94 is ON HOLD Authors: Zhang, Y., Ealick, S.E. Description: Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexe...

New page

'''Unreleased structure'''

The entry 3t94 is ON HOLD

Authors: Zhang, Y., Ealick, S.E.

Description: Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP) II complexed with 5'-deoxy-5'-methylthioadenosine and sulfate