3t9k - Revision history

OCA at 17:30, 1 November 2023

OCA — Wed, 01 Nov 2023 17:30:00 GMT

←Older revision		Revision as of 17:30, 1 November 2023
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	<StructureSection load='3t9k' size='340' side='right'caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3t9k' size='340' side='right'caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ITGB1, FNRB, MDF2, MSK12 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [https://pdbe.org/3t9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [https://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [https://pdbe.org/3t9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [https://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACAP1_HUMAN ACAP1_HUMAN]] GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.<ref>PMID:11062263</ref> <ref>PMID:16256741</ref> <ref>PMID:17398097</ref> <ref>PMID:17664335</ref> <ref>PMID:22645133</ref>	+	[https://www.uniprot.org/uniprot/ITB1_HUMAN ITB1_HUMAN] Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis.<ref>PMID:7523423</ref> <ref>PMID:17956333</ref> <ref>PMID:18804435</ref> [https://www.uniprot.org/uniprot/ACAP1_HUMAN ACAP1_HUMAN] GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.<ref>PMID:11062263</ref> <ref>PMID:16256741</ref> <ref>PMID:17398097</ref> <ref>PMID:17664335</ref> <ref>PMID:22645133</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ma, J]]	+	[[Category: Ma J]]
-	[[Category: Pang, X]]	+	[[Category: Pang X]]
-	[[Category: Sun, F]]	+	[[Category: Sun F]]
-	[[Category: Zhang, K]]	+	[[Category: Zhang K]]
-	[[Category: Zhou, Q]]	+	[[Category: Zhou Q]]
-	~~[[Category: Ank repeat]]~~	+
-	~~[[Category: Arfgap domain]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Zinc-binding module~~]]	+

OCA at 16:48, 6 July 2022

OCA — Wed, 06 Jul 2022 16:48:44 GMT

←Older revision		Revision as of 16:48, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide==		==Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide==
-	<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3t9k' size='340' side='right'caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITGB1, FNRB, MDF2, MSK12 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITGB1, FNRB, MDF2, MSK12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [~~http~~://pdbe.org/3t9k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [https://pdbe.org/3t9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [https://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACAP1_HUMAN ACAP1_HUMAN]] GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.<ref>PMID:11062263</ref> <ref>PMID:16256741</ref> <ref>PMID:17398097</ref> <ref>PMID:17664335</ref> <ref>PMID:22645133</ref>	+	[[https://www.uniprot.org/uniprot/ACAP1_HUMAN ACAP1_HUMAN]] GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.<ref>PMID:11062263</ref> <ref>PMID:16256741</ref> <ref>PMID:17398097</ref> <ref>PMID:17664335</ref> <ref>PMID:22645133</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ma, J]]		[[Category: Ma, J]]
	[[Category: Pang, X]]		[[Category: Pang, X]]

OCA at 09:49, 8 November 2017

OCA — Wed, 08 Nov 2017 09:49:41 GMT

←Older revision		Revision as of 09:49, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ITGB1, FNRB, MDF2, MSK12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://pdbe.org/3t9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://pdbe.org/3t9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Ma, J]]		[[Category: Ma, J]]
	[[Category: Pang, X]]		[[Category: Pang, X]]

OCA at 11:33, 3 August 2017

OCA — Thu, 03 Aug 2017 11:33:00 GMT

←Older revision		Revision as of 11:33, 3 August 2017
Line 3:		Line 3:
	<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human Human]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://pdbe.org/3t9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://pdbe.org/3t9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: Ma, J]]		[[Category: Ma, J]]
	[[Category: Pang, X]]		[[Category: Pang, X]]

OCA at 02:10, 5 August 2016

OCA — Fri, 05 Aug 2016 02:10:58 GMT

←Older revision		Revision as of 02:10, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide==		==Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide==
	<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Synthetic_construct Synthetic construct~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://pdbe.org/3t9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t9k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Synthetic construct~~]]	+	[[Category: Human]]
	[[Category: Ma, J]]		[[Category: Ma, J]]
	[[Category: Pang, X]]		[[Category: Pang, X]]

OCA at 21:55, 24 December 2014

OCA — Wed, 24 Dec 2014 21:55:49 GMT

←Older revision		Revision as of 21:55, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACAP1_HUMAN ACAP1_HUMAN]] GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.<ref>PMID:11062263</ref> <ref>PMID:16256741</ref> <ref>PMID:17398097</ref> <ref>PMID:17664335</ref> <ref>PMID:22645133</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:30, 9 December 2014

OCA — Tue, 09 Dec 2014 13:30:48 GMT

←Older revision		Revision as of 13:30, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3t9k.~~png~~\|~~left\|200px~~]]	+	==Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide==
		+	<StructureSection load='3t9k' size='340' side='right' caption='[[3t9k]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T9K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t9k RCSB], [http://www.ebi.ac.uk/pdbsum/3t9k PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Coat complexes sort protein cargoes into vesicular transport pathways. An emerging class of coat components has been the GAPs (GTPase activating proteins) that act on the ARF (ADP-Ribosylation factor) family of small GTPases. ACAP1 (ArfGAP with Coil coil, Ankyrin repeat, and PH domain protein 1) is an ARF6 GAP that also acts as a key component of a recently defined clathrin complex for endocytic recycling. Phosphorylation by Akt has been shown to enhance cargo binding by ACAP1 in explaining how integrin recycling is an example of regulated transport. We now shed further mechanistic insights into how this regulation is achieved at the level of cargo binding by ACAP1. We initially define a critical sequence in the cytoplasmic domain of integrin beta1 recognized by ACAP1, and show that this sequence acts as a recycling sorting signal. We then pursue a combination of structural, modeling and functional studies, which suggest that phosphorylation of ACAP1 relieves a localized mechanism of auto-inhibition in regulating cargo binding. Thus, we have elucidated a key regulatory juncture that controls integrin recycling, and also advanced the understanding of how regulated cargo binding can lead to regulated transport.

-	~~{{STRUCTURE_3t9k\| PDB=3t9k \| SCENE= }}~~	+	Mechanistic insights into regulated cargo binding by ACAP1.,Bai M, Pang X, Lou J, Zhou Q, Zhang K, Ma J, Li J, Sun F, Hsu V J Biol Chem. 2012 May 29. PMID:22645133<ref>PMID:22645133</ref>

-	~~===Crystal Structure~~ of ~~ACAP1 C-portion mutant S554D fused with integrin beta1 peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22645133}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3t9k]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Synthetic_construct Synthetic construct]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022645133<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]
-	[[Category: Ma, J.]]	+	[[Category: Ma, J]]
-	[[Category: Pang, X.]]	+	[[Category: Pang, X]]
-	[[Category: Sun, F.]]	+	[[Category: Sun, F]]
-	[[Category: Zhang, K.]]	+	[[Category: Zhang, K]]
-	[[Category: Zhou, Q.]]	+	[[Category: Zhou, Q]]
	[[Category: Ank repeat]]		[[Category: Ank repeat]]
	[[Category: Arfgap domain]]		[[Category: Arfgap domain]]
	[[Category: Protein transport]]		[[Category: Protein transport]]
	[[Category: Zinc-binding module]]		[[Category: Zinc-binding module]]

OCA at 09:18, 16 January 2013

OCA — Wed, 16 Jan 2013 09:18:11 GMT

←Older revision		Revision as of 09:18, 16 January 2013
Line 1:		Line 1:
-	[[Image:3t9k.~~jpg~~\|left\|200px]]	+	[[Image:3t9k.png\|left\|200px]]

	{{STRUCTURE_3t9k\| PDB=3t9k \| SCENE= }}		{{STRUCTURE_3t9k\| PDB=3t9k \| SCENE= }}
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens,_synthetic,_homo_sapiens Homo sapiens, synthetic, homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA].	+	[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:022645133</ref><references group="xtra"/>		<ref group="xtra">PMID:022645133</ref><references group="xtra"/>
-	[[Category: ~~Homo sapiens, synthetic, homo sapiens~~]]	+	[[Category: Synthetic construct]]
	[[Category: Ma, J.]]		[[Category: Ma, J.]]
	[[Category: Pang, X.]]		[[Category: Pang, X.]]

OCA at 05:21, 18 July 2012

OCA — Wed, 18 Jul 2012 05:21:14 GMT

←Older revision		Revision as of 05:21, 18 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t9k.jpg\|left\|200px]]

-	~~The entry~~ 3t9k ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3t9k\| PDB=3t9k \| SCENE= }}

-	~~Authors: Sun, F., Pang, X., Zhang, K., Ma, J., Zhou, Q.~~	+	===Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide===

-	~~Description: Crystal~~ Structure ~~of ACAP1 C-portion mutant S554D fused~~ with ~~integrin beta1 peptide~~	+	{{ABSTRACT_PUBMED_22645133}}
		+
		+	==About this Structure==
		+	[[3t9k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_synthetic,_homo_sapiens Homo sapiens, synthetic, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T9K OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022645133</ref><references group="xtra"/>
		+	[[Category: Homo sapiens, synthetic, homo sapiens]]
		+	[[Category: Ma, J.]]
		+	[[Category: Pang, X.]]
		+	[[Category: Sun, F.]]
		+	[[Category: Zhang, K.]]
		+	[[Category: Zhou, Q.]]
		+	[[Category: Ank repeat]]
		+	[[Category: Arfgap domain]]
		+	[[Category: Protein transport]]
		+	[[Category: Zinc-binding module]]

OCA at 07:30, 7 December 2011

OCA — Wed, 07 Dec 2011 07:30:56 GMT

←Older revision		Revision as of 07:30, 7 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t9k is ON HOLD	+	The entry 3t9k is ON HOLD until Paper Publication

	Authors: Sun, F., Pang, X., Zhang, K., Ma, J., Zhou, Q.		Authors: Sun, F., Pang, X., Zhang, K., Ma, J., Zhou, Q.

-	Description: Crystal Structure of mutant S554D ~~for ArfGAP and ANK repeat domain of ACAP1~~	+	Description: Crystal Structure of ACAP1 C-portion mutant S554D fused with integrin beta1 peptide