3ta1 - Revision history

OCA at 13:22, 14 March 2024

OCA — Thu, 14 Mar 2024 13:22:18 GMT

←Older revision		Revision as of 13:22, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3ta1' size='340' side='right'caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3ta1' size='340' side='right'caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arcfu Arcfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [https://pdbe.org/3ta1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ta1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [https://pdbe.org/3ta1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ta1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta1 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/GLNK3_ARCFU GLNK3_ARCFU] Involved in the regulation of nitrogen metabolism (By similarity). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (By similarity). Regulates the activity of the ammonia channel Amt3 via direct interaction (Probable).[UniProtKB:Q60381]<ref>PMID:22039461</ref>
-	GlnK proteins regulate the active uptake of ammonium by Amt transport proteins by inserting their regulatory T-loops into the transport channels of the Amt trimer and physically blocking substrate passage. ~~They sense the cellular nitrogen status through 2-oxoglutarate, and the energy level of the cell by binding both ATP and ADP with different affinities~~. ~~The hyperthermophilic euryarchaeon Archaeoglobus fulgidus possesses three Amt proteins, each encoded~~ in ~~an operon with a GlnK ortholog. One of these proteins, GlnK2 was recently found to be incapable of binding 2-OG, and in order to understand~~ the ~~implications~~ of ~~this finding we conducted a detailed structural and functional analysis of a second GlnK protein from A~~. ~~fulgidus, GlnK3. Contrary to Af-GlnK2 this protein was able to bind both ATP/2-OG and ADP to yield inactive and functional states, respectively. Due to~~ the ~~thermostable nature~~ of ~~the~~ protein ~~we could observe the exact positioning of the notoriously flexible T~~-~~loops and explain the binding behavior of GlnK proteins to their~~ interaction ~~partner, the Amt proteins. A thermodynamic analysis of these binding events using microcalorimetry evaluated by microstate modeling revealed significant differences~~ in ~~binding cooperativity compared~~ to ~~other characterized P(II) proteins, underlining~~ the ~~diversity and adaptability of this class of regulatory signaling proteins.~~	+
-		+
-	~~Mechanism of disruption~~ of the ~~Amt-GlnK complex by P~~(II)~~-mediated sensing~~ of ~~2-oxoglutarate.,Maier S, Schleberger P, Lu W, Wacker T, Pfluger T, Litz C, Andrade SL PLoS One. 2011;6~~(10)~~:e26327~~. ~~Epub 2011 Oct 19. PMID~~:~~22039461~~<ref>PMID:22039461</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ta1" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arcfu~~]]	+	[[Category: Archaeoglobus fulgidus DSM 4304]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andrade~~, S L.A~~]]	+	[[Category: Andrade SLA]]
-	[[Category: Litz, C]]	+	[[Category: Litz C]]
-	[[Category: Lue, W]]	+	[[Category: Lue W]]
-	[[Category: Maier, S]]	+	[[Category: Maier S]]
-	[[Category: Pflueger, T]]	+	[[Category: Pflueger T]]
-	[[Category: Schleberger, P]]	+	[[Category: Schleberger P]]
-	[[Category: Wacker, T]]	+	[[Category: Wacker T]]
-	~~[[Category: Amt3]]~~	+
-	~~[[Category: Pii-family]]~~	+
-	~~[[Category: Regulator]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 16:49, 6 July 2022

OCA — Wed, 06 Jul 2022 16:49:14 GMT

←Older revision		Revision as of 16:49, 6 July 2022
Line 1:		Line 1:

	==A. fulgidus GlnK3, MgADP complex==		==A. fulgidus GlnK3, MgADP complex==
-	<StructureSection load='3ta1' size='340' side='right' caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3ta1' size='340' side='right'caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [~~http~~://pdbe.org/3ta1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ta1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [https://pdbe.org/3ta1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ta1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Arcfu]]		[[Category: Arcfu]]
		+	[[Category: Large Structures]]
	[[Category: Andrade, S L.A]]		[[Category: Andrade, S L.A]]
	[[Category: Litz, C]]		[[Category: Litz, C]]

OCA at 10:51, 11 August 2016

OCA — Thu, 11 Aug 2016 10:51:12 GMT

←Older revision		Revision as of 10:51, 11 August 2016
Line 1:		Line 1:
		+
	==A. fulgidus GlnK3, MgADP complex==		==A. fulgidus GlnK3, MgADP complex==
	<StructureSection load='3ta1' size='340' side='right' caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3ta1' size='340' side='right' caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Archaeoglobus_fulgidus Archaeoglobus fulgidus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~2234 Archaeoglobus fulgidus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [http://pdbe.org/3ta1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ta1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ta1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Archaeoglobus fulgidus~~]]	+	[[Category: Arcfu]]
	[[Category: Andrade, S L.A]]		[[Category: Andrade, S L.A]]
	[[Category: Litz, C]]		[[Category: Litz, C]]

OCA at 09:22, 4 January 2015

OCA — Sun, 04 Jan 2015 09:22:42 GMT

←Older revision		Revision as of 09:22, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.,Maier S, Schleberger P, Lu W, Wacker T, Pfluger T, Litz C, Andrade SL PLoS One. 2011;6(10):e26327. Epub 2011 Oct 19. PMID:22039461<ref>PMID:22039461</ref>		Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.,Maier S, Schleberger P, Lu W, Wacker T, Pfluger T, Litz C, Andrade SL PLoS One. 2011;6(10):e26327. Epub 2011 Oct 19. PMID:22039461<ref>PMID:22039461</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Archaeoglobus fulgidus]]		[[Category: Archaeoglobus fulgidus]]
-	[[Category: Andrade, S L.A.]]	+	[[Category: Andrade, S L.A]]
-	[[Category: Litz, C.]]	+	[[Category: Litz, C]]
-	[[Category: Lue, W.]]	+	[[Category: Lue, W]]
-	[[Category: Maier, S.]]	+	[[Category: Maier, S]]
-	[[Category: Pflueger, T.]]	+	[[Category: Pflueger, T]]
-	[[Category: Schleberger, P.]]	+	[[Category: Schleberger, P]]
-	[[Category: Wacker, T.]]	+	[[Category: Wacker, T]]
	[[Category: Amt3]]		[[Category: Amt3]]
	[[Category: Pii-family]]		[[Category: Pii-family]]
	[[Category: Regulator]]		[[Category: Regulator]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 05:55, 5 June 2014

OCA — Thu, 05 Jun 2014 05:55:05 GMT

←Older revision		Revision as of 05:55, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3ta1.~~png~~\|~~left~~\|~~200px~~]]	+	==A. fulgidus GlnK3, MgADP complex==
		+	<StructureSection load='3ta1' size='340' side='right' caption='[[3ta1]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TA1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t9z\|3t9z]], [[3ta0\|3ta0]], [[3ta2\|3ta2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF_1750, glnK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ta1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ta1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ta1 RCSB], [http://www.ebi.ac.uk/pdbsum/3ta1 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GlnK proteins regulate the active uptake of ammonium by Amt transport proteins by inserting their regulatory T-loops into the transport channels of the Amt trimer and physically blocking substrate passage. They sense the cellular nitrogen status through 2-oxoglutarate, and the energy level of the cell by binding both ATP and ADP with different affinities. The hyperthermophilic euryarchaeon Archaeoglobus fulgidus possesses three Amt proteins, each encoded in an operon with a GlnK ortholog. One of these proteins, GlnK2 was recently found to be incapable of binding 2-OG, and in order to understand the implications of this finding we conducted a detailed structural and functional analysis of a second GlnK protein from A. fulgidus, GlnK3. Contrary to Af-GlnK2 this protein was able to bind both ATP/2-OG and ADP to yield inactive and functional states, respectively. Due to the thermostable nature of the protein we could observe the exact positioning of the notoriously flexible T-loops and explain the binding behavior of GlnK proteins to their interaction partner, the Amt proteins. A thermodynamic analysis of these binding events using microcalorimetry evaluated by microstate modeling revealed significant differences in binding cooperativity compared to other characterized P(II) proteins, underlining the diversity and adaptability of this class of regulatory signaling proteins.

-	<!--	+	Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.,Maier S, Schleberger P, Lu W, Wacker T, Pfluger T, Litz C, Andrade SL PLoS One. 2011;6(10):e26327. Epub 2011 Oct 19. PMID:22039461<ref>PMID:22039461</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3ta1"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3ta1\| PDB=3ta1 \| SCENE= }}~~	+

-	~~===A. fulgidus GlnK3~~, ~~MgADP complex===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22039461}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22039461 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22039461}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ta1]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022039461<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Archaeoglobus fulgidus]]		[[Category: Archaeoglobus fulgidus]]
	[[Category: Andrade, S L.A.]]		[[Category: Andrade, S L.A.]]

OCA at 06:41, 28 March 2012

OCA — Wed, 28 Mar 2012 06:41:05 GMT

←Older revision		Revision as of 06:41, 28 March 2012
Line 11:		Line 11:
	===A. fulgidus GlnK3, MgADP complex===		===A. fulgidus GlnK3, MgADP complex===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22039461}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22039461 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22039461}}

	==About this Structure==		==About this Structure==
	[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA].		[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022039461</ref><references group="xtra"/>
	[[Category: Archaeoglobus fulgidus]]		[[Category: Archaeoglobus fulgidus]]
	[[Category: Andrade, S L.A.]]		[[Category: Andrade, S L.A.]]

OCA at 11:26, 30 October 2011

OCA — Sun, 30 Oct 2011 11:26:07 GMT

←Older revision		Revision as of 11:26, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ta1.png\|left\|200px]]

-	The ~~entry 3ta1~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ta1", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ta1\| PDB=3ta1 \| SCENE= }}

-	~~Authors: Maier, S~~., ~~Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A.~~	+	===A. fulgidus GlnK3, MgADP complex===

-	~~Description~~: A. fulgidus ~~GlnK3~~, ~~MgADP complex~~	+
		+	==About this Structure==
		+	[[3ta1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TA1 OCA].
		+	[[Category: Archaeoglobus fulgidus]]
		+	[[Category: Andrade, S L.A.]]
		+	[[Category: Litz, C.]]
		+	[[Category: Lue, W.]]
		+	[[Category: Maier, S.]]
		+	[[Category: Pflueger, T.]]
		+	[[Category: Schleberger, P.]]
		+	[[Category: Wacker, T.]]
		+	[[Category: Amt3]]
		+	[[Category: Pii-family]]
		+	[[Category: Regulator]]
		+	[[Category: Signaling protein]]

OCA at 06:02, 14 September 2011

OCA — Wed, 14 Sep 2011 06:02:37 GMT

←Older revision		Revision as of 06:02, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ta1 is ON HOLD	+	The entry 3ta1 is ON HOLD until Paper Publication

	Authors: Maier, S., Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A.		Authors: Maier, S., Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A.

	Description: A. fulgidus GlnK3, MgADP complex		Description: A. fulgidus GlnK3, MgADP complex

OCA: Protected "3ta1" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:38:46 GMT

Protected "3ta1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:38, 10 August 2011

OCA: New page: '''Unreleased structure''' The entry 3ta1 is ON HOLD Authors: Maier, S., Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A. Description: A. fulgidus GlnK3, M...

OCA — Wed, 10 Aug 2011 05:38:45 GMT

New page: '''Unreleased structure''' The entry 3ta1 is ON HOLD Authors: Maier, S., Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A. Description: A. fulgidus GlnK3, M...

New page

'''Unreleased structure'''

The entry 3ta1 is ON HOLD

Authors: Maier, S., Schleberger, P., Lue, W., Wacker, T., Pflueger, T., Litz, C., Andrade, S.L.A.

Description: A. fulgidus GlnK3, MgADP complex