3tcl - Revision history

OCA at 13:24, 14 March 2024

OCA — Thu, 14 Mar 2024 13:24:02 GMT

←Older revision		Revision as of 13:24, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3tcl' size='340' side='right'caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='3tcl' size='340' side='right'caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TCL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TCL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.906Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [https://pdbe.org/3tcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [https://www.ebi.ac.uk/pdbsum/3tcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tcl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [https://pdbe.org/3tcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [https://www.ebi.ac.uk/pdbsum/3tcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tcl ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Variable regions 1 and 2 (V1/V2) of human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein are critical for viral evasion of antibody neutralization, and are themselves protected by extraordinary sequence diversity and N-linked glycosylation. Human antibodies such as PG9 nonetheless engage V1/V2 and neutralize 80% of HIV-1 isolates. Here we report the structure of V1/V2 in complex with PG9. V1/V2 forms a four-stranded beta-sheet domain, in which sequence diversity and glycosylation are largely segregated to strand-connecting loops. PG9 recognition involves electrostatic, sequence-independent and glycan interactions: the latter account for over half the interactive surface but are of sufficiently weak affinity to avoid autoreactivity. The structures of V1/V2-directed antibodies CH04 and PGT145 indicate that they share a common mode of glycan penetration by extended anionic loops. In addition to structurally defining V1/V2, the results thus identify a paradigm of antibody recognition for highly glycosylated antigens, which-with PG9-involves a site of vulnerability comprising just two glycans and a strand.
-
-	Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.,McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O'Dell S, Patel N, Shahzad-Ul-Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, Georgiev I, Do Kwon Y, Lee D, Louder MK, Moquin S, Schmidt SD, Yang ZY, Bonsignori M, Crump JA, Kapiga SH, Sam NE, Haynes BF, Burton DR, Koff WC, Walker LM, Phogat S, Wyatt R, Orwenyo J, Wang LX, Arthos J, Bewley CA, Mascola JR, Nabel GJ, Schief WR, Ward AB, Wilson IA, Kwong PD Nature. 2011 Nov 23. doi: 10.1038/nature10696. PMID:22113616<ref>PMID:22113616</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3tcl" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bonsignori, M]]	+	[[Category: Bonsignori M]]
-	[[Category: Kwong~~, P D~~]]	+	[[Category: Kwong PD]]
-	[[Category: Louder~~, R K~~]]	+	[[Category: Louder RK]]
-	[[Category: McLellan~~, J S~~]]	+	[[Category: McLellan JS]]
-	[[Category: Pancera, M]]	+	[[Category: Pancera M]]
-	[[Category: Yang, Y]]	+	[[Category: Yang Y]]
-	[[Category: Zhang, B]]	+	[[Category: Zhang B]]
-	~~[[Category: Ig]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 16:52, 6 July 2022

OCA — Wed, 06 Jul 2022 16:52:04 GMT

←Older revision		Revision as of 16:52, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of HIV-1 Neutralizing Antibody CH04==		==Crystal Structure of HIV-1 Neutralizing Antibody CH04==
-	<StructureSection load='3tcl' size='340' side='right' caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>	+	<StructureSection load='3tcl' size='340' side='right'caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TCL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TCL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [~~http~~://pdbe.org/3tcl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tcl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tcl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [https://pdbe.org/3tcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [https://www.ebi.ac.uk/pdbsum/3tcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tcl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Bonsignori, M]]		[[Category: Bonsignori, M]]
	[[Category: Kwong, P D]]		[[Category: Kwong, P D]]

OCA at 10:31, 11 August 2016

OCA — Thu, 11 Aug 2016 10:31:44 GMT

←Older revision		Revision as of 10:31, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of HIV-1 Neutralizing Antibody CH04==		==Crystal Structure of HIV-1 Neutralizing Antibody CH04==
	<StructureSection load='3tcl' size='340' side='right' caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='3tcl' size='340' side='right' caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TCL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TCL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [http://www.ebi.ac.uk/pdbsum/3tcl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [http://pdbe.org/3tcl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [http://www.ebi.ac.uk/pdbsum/3tcl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tcl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tcl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Bonsignori, M]]		[[Category: Bonsignori, M]]
	[[Category: Kwong, P D]]		[[Category: Kwong, P D]]

OCA at 10:59, 4 January 2015

OCA — Sun, 04 Jan 2015 10:59:33 GMT

←Older revision		Revision as of 10:59, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TCL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TCL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [http://www.ebi.ac.uk/pdbsum/3tcl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [http://www.ebi.ac.uk/pdbsum/3tcl PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.,McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O'Dell S, Patel N, Shahzad-Ul-Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, Georgiev I, Do Kwon Y, Lee D, Louder MK, Moquin S, Schmidt SD, Yang ZY, Bonsignori M, Crump JA, Kapiga SH, Sam NE, Haynes BF, Burton DR, Koff WC, Walker LM, Phogat S, Wyatt R, Orwenyo J, Wang LX, Arthos J, Bewley CA, Mascola JR, Nabel GJ, Schief WR, Ward AB, Wilson IA, Kwong PD Nature. 2011 Nov 23. doi: 10.1038/nature10696. PMID:22113616<ref>PMID:22113616</ref>		Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.,McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O'Dell S, Patel N, Shahzad-Ul-Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, Georgiev I, Do Kwon Y, Lee D, Louder MK, Moquin S, Schmidt SD, Yang ZY, Bonsignori M, Crump JA, Kapiga SH, Sam NE, Haynes BF, Burton DR, Koff WC, Walker LM, Phogat S, Wyatt R, Orwenyo J, Wang LX, Arthos J, Bewley CA, Mascola JR, Nabel GJ, Schief WR, Ward AB, Wilson IA, Kwong PD Nature. 2011 Nov 23. doi: 10.1038/nature10696. PMID:22113616<ref>PMID:22113616</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Bonsignori, M.]]	+	[[Category: Bonsignori, M]]
-	[[Category: Kwong, P D.]]	+	[[Category: Kwong, P D]]
-	[[Category: Louder, R K.]]	+	[[Category: Louder, R K]]
-	[[Category: McLellan, J S.]]	+	[[Category: McLellan, J S]]
-	[[Category: Pancera, M.]]	+	[[Category: Pancera, M]]
-	[[Category: Yang, Y.]]	+	[[Category: Yang, Y]]
-	[[Category: Zhang, B.]]	+	[[Category: Zhang, B]]
	[[Category: Ig]]		[[Category: Ig]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 06:38, 5 June 2014

OCA — Thu, 05 Jun 2014 06:38:39 GMT

←Older revision		Revision as of 06:38, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tcl.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of HIV-1 Neutralizing Antibody CH04==
		+	<StructureSection load='3tcl' size='340' side='right' caption='[[3tcl]], [[Resolution\|resolution]] 1.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TCL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u2s\|3u2s]], [[3u46\|3u46]], [[3u36\|3u36]], [[3u4b\|3u4b]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tcl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tcl RCSB], [http://www.ebi.ac.uk/pdbsum/3tcl PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Variable regions 1 and 2 (V1/V2) of human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein are critical for viral evasion of antibody neutralization, and are themselves protected by extraordinary sequence diversity and N-linked glycosylation. Human antibodies such as PG9 nonetheless engage V1/V2 and neutralize 80% of HIV-1 isolates. Here we report the structure of V1/V2 in complex with PG9. V1/V2 forms a four-stranded beta-sheet domain, in which sequence diversity and glycosylation are largely segregated to strand-connecting loops. PG9 recognition involves electrostatic, sequence-independent and glycan interactions: the latter account for over half the interactive surface but are of sufficiently weak affinity to avoid autoreactivity. The structures of V1/V2-directed antibodies CH04 and PGT145 indicate that they share a common mode of glycan penetration by extended anionic loops. In addition to structurally defining V1/V2, the results thus identify a paradigm of antibody recognition for highly glycosylated antigens, which-with PG9-involves a site of vulnerability comprising just two glycans and a strand.

-	<!--	+	Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.,McLellan JS, Pancera M, Carrico C, Gorman J, Julien JP, Khayat R, Louder R, Pejchal R, Sastry M, Dai K, O'Dell S, Patel N, Shahzad-Ul-Hussan S, Yang Y, Zhang B, Zhou T, Zhu J, Boyington JC, Chuang GY, Diwanji D, Georgiev I, Do Kwon Y, Lee D, Louder MK, Moquin S, Schmidt SD, Yang ZY, Bonsignori M, Crump JA, Kapiga SH, Sam NE, Haynes BF, Burton DR, Koff WC, Walker LM, Phogat S, Wyatt R, Orwenyo J, Wang LX, Arthos J, Bewley CA, Mascola JR, Nabel GJ, Schief WR, Ward AB, Wilson IA, Kwong PD Nature. 2011 Nov 23. doi: 10.1038/nature10696. PMID:22113616<ref>PMID:22113616</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tcl"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3tcl\| PDB=3tcl \| SCENE= }}~~	+

-	~~===Crystal Structure~~ of ~~HIV-1 Neutralizing Antibody CH04===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22113616}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22113616 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22113616}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tcl]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022113616<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Bonsignori, M.]]		[[Category: Bonsignori, M.]]

OCA at 10:40, 14 December 2011

OCA — Wed, 14 Dec 2011 10:40:32 GMT

←Older revision		Revision as of 10:40, 14 December 2011
Line 11:		Line 11:
	===Crystal Structure of HIV-1 Neutralizing Antibody CH04===		===Crystal Structure of HIV-1 Neutralizing Antibody CH04===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22113616}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22113616 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22113616}}

	==About this Structure==		==About this Structure==
	[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA].		[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022113616</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Bonsignori, M.]]		[[Category: Bonsignori, M.]]

OCA at 08:10, 7 December 2011

OCA — Wed, 07 Dec 2011 08:10:35 GMT

←Older revision		Revision as of 08:10, 7 December 2011
Line 1:		Line 1:
-	[[Image:3tcl.~~jpg~~\|left\|200px]]	+	[[Image:3tcl.png\|left\|200px]]

	<!--		<!--

OCA at 06:28, 30 November 2011

OCA — Wed, 30 Nov 2011 06:28:13 GMT

←Older revision		Revision as of 06:28, 30 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tcl.jpg\|left\|200px]]

-	The ~~entry 3tcl~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tcl", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tcl\| PDB=3tcl \| SCENE= }}

-	~~Authors: Louder, R.K., McLellan, J.S., Pancera, M., Yang, Y., Zhang, B., Bonsignori, M., Kwong, P.D.~~	+	===Crystal Structure of HIV-1 Neutralizing Antibody CH04===

-	~~Description: Crystal~~ Structure ~~of HIV~~-~~1 Neutralizing Antibody CH04~~	+
		+	==About this Structure==
		+	[[3tcl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TCL OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Bonsignori, M.]]
		+	[[Category: Kwong, P D.]]
		+	[[Category: Louder, R K.]]
		+	[[Category: McLellan, J S.]]
		+	[[Category: Pancera, M.]]
		+	[[Category: Yang, Y.]]
		+	[[Category: Zhang, B.]]
		+	[[Category: Ig]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]

OCA at 06:03, 14 September 2011

OCA — Wed, 14 Sep 2011 06:03:12 GMT

←Older revision		Revision as of 06:03, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tcl is ON HOLD	+	The entry 3tcl is ON HOLD until Paper Publication

	Authors: Louder, R.K., McLellan, J.S., Pancera, M., Yang, Y., Zhang, B., Bonsignori, M., Kwong, P.D.		Authors: Louder, R.K., McLellan, J.S., Pancera, M., Yang, Y., Zhang, B., Bonsignori, M., Kwong, P.D.

	Description: Crystal Structure of HIV-1 Neutralizing Antibody CH04		Description: Crystal Structure of HIV-1 Neutralizing Antibody CH04

OCA: Protected "3tcl" [edit=sysop:move=sysop]

OCA — Wed, 17 Aug 2011 05:57:17 GMT

Protected "3tcl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:57, 17 August 2011