3tfz - Revision history

OCA at 10:08, 1 March 2024

OCA — Fri, 01 Mar 2024 10:08:55 GMT

←Older revision		Revision as of 10:08, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tfz' size='340' side='right'caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>		<StructureSection load='3tfz' size='340' side='right'caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp.~~_r1128~~ Streptomyces sp. ~~r1128~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._R1128 Streptomyces sp. R1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CXS:3~~-~~CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>~~, ~~<scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.39Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~zhuI ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=140437 Streptomyces sp. R1128])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [https://pdbe.org/3tfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [https://www.ebi.ac.uk/pdbsum/3tfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tfz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [https://pdbe.org/3tfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [https://www.ebi.ac.uk/pdbsum/3tfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tfz ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9F6D3_9ACTN Q9F6D3_9ACTN]
-	Aromatic polyketides comprise an important class of natural products that possess a wide range of biological activities. The cyclization of the polyketide chain is a critical control point in the biosynthesis of aromatic polyketides. The aromatase/cyclases (ARO/CYCs) are an important component of the type II polyketide synthase (PKS) and help fold the polyketide for regiospecific cyclizations of the first ring and/or aromatization, promoting two commonly observed first-ring cyclization patterns for the bacterial type II PKSs: ~~C7-C12 and C9-C14. We had previously reported the crystal structure and enzymological analyses of the TcmN ARO~~/CYC, which promotes C9-C14 first-ring cyclization. However, how C7-C12 first-ring cyclization is controlled remains unresolved. In this work, we present the 2.4 A crystal structure of ZhuI, a C7-C12-specific first-ring ARO/~~CYC from the type II PKS pathway responsible for the production of the R1128 polyketides~~. Though ZhuI possesses a helix-grip fold shared by TcmN ARO/CYC, there are substantial differences in overall structure and pocket residue composition that may be important for directing C7-C12 (rather than C9-C14) cyclization. Docking studies and site-directed mutagenesis coupled to an in vitro activity assay demonstrate that ZhuI pocket residues R66, H109, and D146 are important for enzyme function. The ZhuI crystal structure helps visualize the structure and putative dehydratase function of the didomain ARO/CYCs from KR-containing type II PKSs. The sequence-structure-function analysis described for ZhuI elucidates the molecular mechanisms that control C7-C12 first-ring polyketide cyclization and builds a foundation for future endeavors into directing cyclization patterns for engineered biosynthesis of aromatic polyketides.	+
-		+
-	Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.,Ames BD, Lee MY, Moody C, Zhang W, Tang Y, Tsai SC Biochemistry. 2011 Sep 8. PMID:21870821<ref>PMID:21870821</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tfz" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~r1128~~]]	+	[[Category: Streptomyces sp. R1128]]
-	[[Category: Ames~~, B D~~]]	+	[[Category: Ames BD]]
-	[[Category: Lee~~, M Y~~]]	+	[[Category: Lee MY]]
-	[[Category: Moody, C]]	+	[[Category: Moody C]]
-	[[Category: Tang, Y]]	+	[[Category: Tang Y]]
-	[[Category: Tsai~~, S C~~]]	+	[[Category: Tsai SC]]
-	[[Category: Wong~~, S K~~]]	+	[[Category: Wong SK]]
-	[[Category: Zhang, W]]	+	[[Category: Zhang W]]
-	~~[[Category: Aromatic polyketide cyclase/dehydrase]]~~	+
-	~~[[Category: Bet v1-like superfamily]]~~	+
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Helix-grip~~]]	+

OCA at 16:56, 6 July 2022

OCA — Wed, 06 Jul 2022 16:56:28 GMT

←Older revision		Revision as of 16:56, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==		==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==
-	<StructureSection load='3tfz' size='340' side='right' caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>	+	<StructureSection load='3tfz' size='340' side='right'caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [~~http~~://pdbe.org/3tfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tfz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [https://pdbe.org/3tfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [https://www.ebi.ac.uk/pdbsum/3tfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tfz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptomyces sp. r1128]]		[[Category: Streptomyces sp. r1128]]
	[[Category: Ames, B D]]		[[Category: Ames, B D]]

OCA at 19:34, 11 August 2016

OCA — Thu, 11 Aug 2016 19:34:10 GMT

←Older revision		Revision as of 19:34, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==		==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==
	<StructureSection load='3tfz' size='340' side='right' caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>		<StructureSection load='3tfz' size='340' side='right' caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://pdbe.org/3tfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tfz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tfz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:08, 4 January 2015

OCA — Sun, 04 Jan 2015 09:08:51 GMT

←Older revision		Revision as of 09:08, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.,Ames BD, Lee MY, Moody C, Zhang W, Tang Y, Tsai SC Biochemistry. 2011 Sep 8. PMID:21870821<ref>PMID:21870821</ref>		Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.,Ames BD, Lee MY, Moody C, Zhang W, Tang Y, Tsai SC Biochemistry. 2011 Sep 8. PMID:21870821<ref>PMID:21870821</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces sp. r1128]]		[[Category: Streptomyces sp. r1128]]
-	[[Category: Ames, B D.]]	+	[[Category: Ames, B D]]
-	[[Category: Lee, M Y.]]	+	[[Category: Lee, M Y]]
-	[[Category: Moody, C.]]	+	[[Category: Moody, C]]
-	[[Category: Tang, Y.]]	+	[[Category: Tang, Y]]
-	[[Category: Tsai, S C.]]	+	[[Category: Tsai, S C]]
-	[[Category: Wong, S K.]]	+	[[Category: Wong, S K]]
-	[[Category: Zhang, W.]]	+	[[Category: Zhang, W]]
	[[Category: Aromatic polyketide cyclase/dehydrase]]		[[Category: Aromatic polyketide cyclase/dehydrase]]
	[[Category: Bet v1-like superfamily]]		[[Category: Bet v1-like superfamily]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Helix-grip]]		[[Category: Helix-grip]]

OCA at 05:40, 5 June 2014

OCA — Thu, 05 Jun 2014 05:40:57 GMT

←Older revision		Revision as of 05:40, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tfz.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128==
		+	<StructureSection load='3tfz' size='340' side='right' caption='[[3tfz]], [[Resolution\|resolution]] 2.39Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TFZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">zhuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=140437 Streptomyces sp. R1128])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tfz RCSB], [http://www.ebi.ac.uk/pdbsum/3tfz PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aromatic polyketides comprise an important class of natural products that possess a wide range of biological activities. The cyclization of the polyketide chain is a critical control point in the biosynthesis of aromatic polyketides. The aromatase/cyclases (ARO/CYCs) are an important component of the type II polyketide synthase (PKS) and help fold the polyketide for regiospecific cyclizations of the first ring and/or aromatization, promoting two commonly observed first-ring cyclization patterns for the bacterial type II PKSs: C7-C12 and C9-C14. We had previously reported the crystal structure and enzymological analyses of the TcmN ARO/CYC, which promotes C9-C14 first-ring cyclization. However, how C7-C12 first-ring cyclization is controlled remains unresolved. In this work, we present the 2.4 A crystal structure of ZhuI, a C7-C12-specific first-ring ARO/CYC from the type II PKS pathway responsible for the production of the R1128 polyketides. Though ZhuI possesses a helix-grip fold shared by TcmN ARO/CYC, there are substantial differences in overall structure and pocket residue composition that may be important for directing C7-C12 (rather than C9-C14) cyclization. Docking studies and site-directed mutagenesis coupled to an in vitro activity assay demonstrate that ZhuI pocket residues R66, H109, and D146 are important for enzyme function. The ZhuI crystal structure helps visualize the structure and putative dehydratase function of the didomain ARO/CYCs from KR-containing type II PKSs. The sequence-structure-function analysis described for ZhuI elucidates the molecular mechanisms that control C7-C12 first-ring polyketide cyclization and builds a foundation for future endeavors into directing cyclization patterns for engineered biosynthesis of aromatic polyketides.

-	~~<!--~~	+	Structural and Biochemical Characterization of ZhuI Aromatase/Cyclase from the R1128 Polyketide Pathway.,Ames BD, Lee MY, Moody C, Zhang W, Tang Y, Tsai SC Biochemistry. 2011 Sep 8. PMID:21870821<ref>PMID:21870821</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3tfz", creates the "Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3tfz\| PDB=3tfz \| SCENE= }}~~	+

-	~~===Crystal structure of Zhui aromatase~~/~~cyclase from Streptomcyes sp. R1128===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21870821}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21870821 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21870821}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tfz]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021870821<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces sp. r1128]]		[[Category: Streptomyces sp. r1128]]
	[[Category: Ames, B D.]]		[[Category: Ames, B D.]]

OCA at 07:11, 30 October 2011

OCA — Sun, 30 Oct 2011 07:11:47 GMT

←Older revision		Revision as of 07:11, 30 October 2011
Line 1:		Line 1:
-	[[Image:3tfz.~~jpg~~\|left\|200px]]	+	[[Image:3tfz.png\|left\|200px]]

	<!--		<!--

OCA at 06:23, 14 September 2011

OCA — Wed, 14 Sep 2011 06:23:54 GMT

←Older revision		Revision as of 06:23, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tfz.jpg\|left\|200px]]

-	The ~~entry 3tfz~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tfz", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tfz\| PDB=3tfz \| SCENE= }}

-	~~Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C~~.	+	===Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128===

-	~~Description~~: ~~Crystal~~ structure ~~of Zhui aromatase~~/~~cyclase~~ from ~~Streptomcyes~~ sp. ~~R1128~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21870821}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21870821 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21870821}}
		+
		+	==About this Structure==
		+	[[3tfz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._r1128 Streptomyces sp. r1128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TFZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021870821</ref><references group="xtra"/>
		+	[[Category: Streptomyces sp. r1128]]
		+	[[Category: Ames, B D.]]
		+	[[Category: Lee, M Y.]]
		+	[[Category: Moody, C.]]
		+	[[Category: Tang, Y.]]
		+	[[Category: Tsai, S C.]]
		+	[[Category: Wong, S K.]]
		+	[[Category: Zhang, W.]]
		+	[[Category: Aromatic polyketide cyclase/dehydrase]]
		+	[[Category: Bet v1-like superfamily]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Helix-grip]]

OCA at 06:24, 31 August 2011

OCA — Wed, 31 Aug 2011 06:24:25 GMT

←Older revision		Revision as of 06:24, 31 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tfz is ON HOLD	+	The entry 3tfz is ON HOLD until Paper Publication

	Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C.		Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C.

-	Description: ~~CRYSTAL STRUCTURE OF ZHUI AROMATASE~~/~~CYCLASE FROM STREPTOMCYES SP~~. R1128	+	Description: Crystal structure of Zhui aromatase/cyclase from Streptomcyes sp. R1128

OCA: Protected "3tfz" [edit=sysop:move=sysop]

OCA — Wed, 24 Aug 2011 07:12:44 GMT

Protected "3tfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:12, 24 August 2011

OCA: New page: '''Unreleased structure''' The entry 3tfz is ON HOLD Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C. Description: CRYSTAL STRUCTURE OF ZHUI AROMA...

OCA — Wed, 24 Aug 2011 07:12:43 GMT

New page: '''Unreleased structure''' The entry 3tfz is ON HOLD Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C. Description: CRYSTAL STRUCTURE OF ZHUI AROMA...

New page

'''Unreleased structure'''

The entry 3tfz is ON HOLD

Authors: Ames, B.D., Lee, M.Y., Moody, C., Zhang, W., Tang, Y., Wong, S.K., Tsai, S.C.

Description: CRYSTAL STRUCTURE OF ZHUI AROMATASE/CYCLASE FROM STREPTOMCYES SP. R1128