3th6 - Revision history

OCA at 13:28, 14 March 2024

OCA — Thu, 14 Mar 2024 13:28:31 GMT

←Older revision		Revision as of 13:28, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3th6' size='340' side='right'caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3th6' size='340' side='right'caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Boophilus_microplus Boophilus~~ microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>TIM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=6941 Boophilus microplus])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose~~-~~phosphate_isomerase Triose-phosphate isomerase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [https://pdbe.org/3th6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [https://www.ebi.ac.uk/pdbsum/3th6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3th6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [https://pdbe.org/3th6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [https://www.ebi.ac.uk/pdbsum/3th6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3th6 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A8B3A8_RHIMP A8B3A8_RHIMP]
-	Triosephosphate isomerase (TIM) is an enzyme with a role in glycolysis and gluconeogenesis by catalyzing the interconversion between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. This enzyme has been used as a target in endoparasite drug development. In this work we cloned, expressed, purified and studied kinetic and structural characteristics of TIM from tick embryos, Rhipicephalus (Boophilus) microplus (BmTIM). The Km and Vmax of the recombinant BmTIM with glyceraldehyde 3-phosphate as substrate, were 0.47 mM and 6031 mumol min(1) mg protein(1), respectively. The resolution of the diffracted crystal was estimated to be 2.4 A and the overall data showed that BmTIM is similar to other reported dimeric TIMs. However, we found that, in comparison to other TIMs, BmTIM has the highest content of cysteine residues (nine cysteine residues per monomer). Only two cysteines could make disulfide bonds in monomers of BmTIM. Furthermore, BmTIM was highly sensitive to the action of the thiol reagents dithionitrobenzoic acid and methyl methane thiosulfonate, suggesting that there are five cysteines exposed in each dimer and that these residues could be employed in the development of species-specific inhibitors.	+
-		+
-	Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.,Moraes J, Arreola R, Cabrera N, Saramago L, Freitas D, Masuda A, da Silva Vaz I Jr, Tuena de Gomez-Puyou M, Perez-Montfort R, Gomez-Puyou A, Logullo C Insect Biochem Mol Biol. 2011 Jun;41(6):~~400-9. Epub 2011 Mar 9. PMID:21396445<ref>PMID:21396445<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3th6" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Boophilus microplus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Triose-phosphate isomerase~~]]	+	[[Category: Rhipicephalus microplus]]
-	[[Category: Arreola, R]]	+	[[Category: Arreola R]]
-	[[Category: Gomez-Puyou, A]]	+	[[Category: Gomez-Puyou A]]
-	[[Category: Logullo, C]]	+	[[Category: Logullo C]]
-	[[Category: Moraes, J]]	+	[[Category: Moraes J]]
-	[[Category: Perez-Montfort, R]]	+	[[Category: Perez-Montfort R]]
-	[[Category: Rodriguez-Romero, A]]	+	[[Category: Rodriguez-Romero A]]
-	~~[[Category: Alpha/beta barrel]]~~	+
-	~~[[Category: Embryogenesis]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 16:57, 6 July 2022

OCA — Wed, 06 Jul 2022 16:57:55 GMT

←Older revision		Revision as of 16:57, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.==		==Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.==
-	<StructureSection load='3th6' size='340' side='right' caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='3th6' size='340' side='right'caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Boophilus_microplus Boophilus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Boophilus_microplus Boophilus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Boophilus microplus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Boophilus microplus])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [~~http~~://pdbe.org/3th6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3th6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3th6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [https://pdbe.org/3th6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [https://www.ebi.ac.uk/pdbsum/3th6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3th6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Boophilus microplus]]		[[Category: Boophilus microplus]]
		+	[[Category: Large Structures]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Arreola, R]]		[[Category: Arreola, R]]

OCA at 10:31, 11 August 2016

OCA — Thu, 11 Aug 2016 10:31:26 GMT

←Older revision		Revision as of 10:31, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.==		==Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.==
	<StructureSection load='3th6' size='340' side='right' caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3th6' size='340' side='right' caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhipicephalus_microplus Rhipicephalus~~ microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Boophilus_microplus Boophilus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 ~~Rhipicephalus~~ microplus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Boophilus microplus])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [http://www.ebi.ac.uk/pdbsum/3th6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [http://pdbe.org/3th6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [http://www.ebi.ac.uk/pdbsum/3th6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3th6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3th6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rhipicephalus~~ microplus]]	+	[[Category: Boophilus microplus]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Arreola, R]]		[[Category: Arreola, R]]

OCA at 10:59, 4 January 2015

OCA — Sun, 04 Jan 2015 10:59:14 GMT

←Older revision		Revision as of 10:59, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Rhipicephalus microplus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Rhipicephalus microplus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [http://www.ebi.ac.uk/pdbsum/3th6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [http://www.ebi.ac.uk/pdbsum/3th6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.,Moraes J, Arreola R, Cabrera N, Saramago L, Freitas D, Masuda A, da Silva Vaz I Jr, Tuena de Gomez-Puyou M, Perez-Montfort R, Gomez-Puyou A, Logullo C Insect Biochem Mol Biol. 2011 Jun;41(6):400-9. Epub 2011 Mar 9. PMID:21396445<ref>PMID:21396445</ref>		Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.,Moraes J, Arreola R, Cabrera N, Saramago L, Freitas D, Masuda A, da Silva Vaz I Jr, Tuena de Gomez-Puyou M, Perez-Montfort R, Gomez-Puyou A, Logullo C Insect Biochem Mol Biol. 2011 Jun;41(6):400-9. Epub 2011 Mar 9. PMID:21396445<ref>PMID:21396445</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	[[Category: Rhipicephalus microplus]]		[[Category: Rhipicephalus microplus]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
-	[[Category: Arreola, R.]]	+	[[Category: Arreola, R]]
-	[[Category: Gomez-Puyou, A.]]	+	[[Category: Gomez-Puyou, A]]
-	[[Category: Logullo, C.]]	+	[[Category: Logullo, C]]
-	[[Category: Moraes, J.]]	+	[[Category: Moraes, J]]
-	[[Category: Perez-Montfort, R.]]	+	[[Category: Perez-Montfort, R]]
-	[[Category: Rodriguez-Romero, A.]]	+	[[Category: Rodriguez-Romero, A]]
	[[Category: Alpha/beta barrel]]		[[Category: Alpha/beta barrel]]
	[[Category: Embryogenesis]]		[[Category: Embryogenesis]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Isomerase]]		[[Category: Isomerase]]

OCA at 06:38, 5 June 2014

OCA — Thu, 05 Jun 2014 06:38:16 GMT

←Older revision		Revision as of 06:38, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3th6.~~jpg~~\|~~left\|200px~~]]	+	==Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.==
		+	<StructureSection load='3th6' size='340' side='right' caption='[[3th6]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TH6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6941 Rhipicephalus microplus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3th6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3th6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3th6 RCSB], [http://www.ebi.ac.uk/pdbsum/3th6 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Triosephosphate isomerase (TIM) is an enzyme with a role in glycolysis and gluconeogenesis by catalyzing the interconversion between glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. This enzyme has been used as a target in endoparasite drug development. In this work we cloned, expressed, purified and studied kinetic and structural characteristics of TIM from tick embryos, Rhipicephalus (Boophilus) microplus (BmTIM). The Km and Vmax of the recombinant BmTIM with glyceraldehyde 3-phosphate as substrate, were 0.47 mM and 6031 mumol min(1) mg protein(1), respectively. The resolution of the diffracted crystal was estimated to be 2.4 A and the overall data showed that BmTIM is similar to other reported dimeric TIMs. However, we found that, in comparison to other TIMs, BmTIM has the highest content of cysteine residues (nine cysteine residues per monomer). Only two cysteines could make disulfide bonds in monomers of BmTIM. Furthermore, BmTIM was highly sensitive to the action of the thiol reagents dithionitrobenzoic acid and methyl methane thiosulfonate, suggesting that there are five cysteines exposed in each dimer and that these residues could be employed in the development of species-specific inhibitors.

-	<!--	+	Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.,Moraes J, Arreola R, Cabrera N, Saramago L, Freitas D, Masuda A, da Silva Vaz I Jr, Tuena de Gomez-Puyou M, Perez-Montfort R, Gomez-Puyou A, Logullo C Insect Biochem Mol Biol. 2011 Jun;41(6):400-9. Epub 2011 Mar 9. PMID:21396445<ref>PMID:21396445</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3th6"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3th6\| PDB=3th6 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21396445}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21396445 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21396445}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3th6]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021396445<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Rhipicephalus microplus]]		[[Category: Rhipicephalus microplus]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]

OCA at 06:44, 31 August 2011

OCA — Wed, 31 Aug 2011 06:44:35 GMT

←Older revision		Revision as of 06:44, 31 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3th6.jpg\|left\|200px]]

-	The ~~entry 3th6~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3th6", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3th6\| PDB=3th6 \| SCENE= }}

-	~~Authors: Arreola, R., Rodriguez-Romero, A., Moraes, J., Gomez-Puyou, A., Perez-Montfort, R., Logullo, C~~.	+	===Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.===

-	~~Description~~: ~~Crystal~~ structure ~~of Triosephosphate isomerase~~ from Rhipicephalus ~~(Boophilus)~~ microplus.	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21396445}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21396445 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21396445}}
		+
		+	==About this Structure==
		+	[[3th6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhipicephalus_microplus Rhipicephalus microplus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TH6 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021396445</ref><references group="xtra"/>
		+	[[Category: Rhipicephalus microplus]]
		+	[[Category: Triose-phosphate isomerase]]
		+	[[Category: Arreola, R.]]
		+	[[Category: Gomez-Puyou, A.]]
		+	[[Category: Logullo, C.]]
		+	[[Category: Moraes, J.]]
		+	[[Category: Perez-Montfort, R.]]
		+	[[Category: Rodriguez-Romero, A.]]
		+	[[Category: Alpha/beta barrel]]
		+	[[Category: Embryogenesis]]
		+	[[Category: Glycolysis]]
		+	[[Category: Isomerase]]

OCA: Protected "3th6" [edit=sysop:move=sysop]

OCA — Wed, 24 Aug 2011 07:13:50 GMT

Protected "3th6" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:13, 24 August 2011

OCA: New page: '''Unreleased structure''' The entry 3th6 is ON HOLD Authors: Arreola, R., Rodriguez-Romero, A., Moraes, J., Gomez-Puyou, A., Perez-Montfort, R., Logullo, C. Description: Crystal struc...

OCA — Wed, 24 Aug 2011 07:13:50 GMT

New page: '''Unreleased structure''' The entry 3th6 is ON HOLD Authors: Arreola, R., Rodriguez-Romero, A., Moraes, J., Gomez-Puyou, A., Perez-Montfort, R., Logullo, C. Description: Crystal struc...

New page

'''Unreleased structure'''

The entry 3th6 is ON HOLD

Authors: Arreola, R., Rodriguez-Romero, A., Moraes, J., Gomez-Puyou, A., Perez-Montfort, R., Logullo, C.

Description: Crystal structure of Triosephosphate isomerase from Rhipicephalus (Boophilus) microplus.