3tiv - Revision history

OCA at 17:32, 1 November 2023

OCA — Wed, 01 Nov 2023 17:32:53 GMT

←Older revision		Revision as of 17:32, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3tiv' size='340' side='right'caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3tiv' size='340' side='right'caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Metma Metma~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei_Go1 Methanosarcina mazei Go1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192952 METMA])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [https://pdbe.org/3tiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [https://www.ebi.ac.uk/pdbsum/3tiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tiv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [https://pdbe.org/3tiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [https://www.ebi.ac.uk/pdbsum/3tiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tiv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VATB_METMA VATB_METMA]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.	+	[https://www.uniprot.org/uniprot/VATB_METMA VATB_METMA] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	A strategically placed tryptophan in position of Arg416 was used as an optical probe to monitor adenosine triphosphate and adenosine-diphosphate binding to subunit B of the A(1)A(O) adenosine triphosphate (ATP) synthase from Methanosarcina mazei Go1. Tryptophan fluorescence and fluorescence correlation spectroscopy gave binding constants indicating a preferred binding of ATP over ADP to the protein. The X-ray crystal structure of the R416W mutant protein in the presence of ATP was solved to 2.1 A resolution, showing the substituted Trp-residue inside the predicted adenine-binding pocket. The cocrystallized ATP molecule could be trapped in a so-called transition nucleotide-binding state. The high resolution structure shows the phosphate residues of the ATP near the P-loop region (S150-E158) and its adenine ring forms pi-pi interaction with Phe149. This transition binding position of ATP could be confirmed by tryptophan emission spectra using the subunit B mutant F149W. The trapped ATP position, similar to the one of the binding region of the antibiotic efrapeptin in F(1)F(O) ATP synthases, is discussed in light of a transition nucleotide-binding state of ATP while on its way to the final binding pocket. Finally, the inhibitory effect of efrapeptin C in ATPase activity of a reconstituted A(3)B(3)- and A(3)B(R416W)(3)-subcomplex, composed of subunit A and the B subunit mutant R416W, of the A(1)A(O) ATP synthase is shown.	+
-		+
-	Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase.,Kumar A, Manimekalai MS, Balakrishna AM, Hunke C, Weigelt S, Sewald N, Gruber G Proteins. 2009 Jun;75(4):807-19. PMID:19003877<ref>PMID:19003877</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tiv" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[ATPase 3D structures\|ATPase 3D structures]]		*[[ATPase 3D structures\|ATPase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Metma~~]]	+	[[Category: Methanosarcina mazei Go1]]
-	[[Category: Gruber, G]]	+	[[Category: Gruber G]]
-	[[Category: Jeyakanthan, J]]	+	[[Category: Jeyakanthan J]]
-	[[Category: Manimekalai~~, M S.S~~]]	+	[[Category: Manimekalai MSS]]
-	[[Category: Tadwal~~, V S]]~~	+	[[Category: Tadwal VS]]
-	~~[[Category: Atp synthesis]]~~	+
-	~~[[Category: Hydrogen ion transport]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 17:00, 6 July 2022

OCA — Wed, 06 Jul 2022 17:00:05 GMT

←Older revision		Revision as of 17:00, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of subunit B mutant N157A of the A1AO ATP synthase==		==Crystal structure of subunit B mutant N157A of the A1AO ATP synthase==
-	<StructureSection load='3tiv' size='340' side='right' caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3tiv' size='340' side='right'caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Metma Metma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TIV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metma Metma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192952 METMA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192952 METMA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [~~http~~://pdbe.org/3tiv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tiv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tiv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [https://pdbe.org/3tiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [https://www.ebi.ac.uk/pdbsum/3tiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tiv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VATB_METMA VATB_METMA]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.	+	[[https://www.uniprot.org/uniprot/VATB_METMA VATB_METMA]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[ATPase\|ATPase]]	+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Metma]]		[[Category: Metma]]
	[[Category: Gruber, G]]		[[Category: Gruber, G]]

OCA at 14:42, 5 August 2016

OCA — Fri, 05 Aug 2016 14:42:22 GMT

←Older revision		Revision as of 14:42, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of subunit B mutant N157A of the A1AO ATP synthase==		==Crystal structure of subunit B mutant N157A of the A1AO ATP synthase==
	<StructureSection load='3tiv' size='340' side='right' caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3tiv' size='340' side='right' caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methanosarcina_mazei Methanosarcina mazei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metma Metma]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~2209 Methanosarcina mazei~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192952 METMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [http://www.ebi.ac.uk/pdbsum/3tiv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [http://pdbe.org/3tiv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [http://www.ebi.ac.uk/pdbsum/3tiv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tiv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tiv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Methanosarcina mazei~~]]	+	[[Category: Metma]]
	[[Category: Gruber, G]]		[[Category: Gruber, G]]
	[[Category: Jeyakanthan, J]]		[[Category: Jeyakanthan, J]]

OCA at 03:20, 25 December 2014

OCA — Thu, 25 Dec 2014 03:20:12 GMT

←Older revision		Revision as of 03:20, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2209 Methanosarcina mazei])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2209 Methanosarcina mazei])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [http://www.ebi.ac.uk/pdbsum/3tiv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [http://www.ebi.ac.uk/pdbsum/3tiv PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VATB_METMA VATB_METMA]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:

	==See Also==		==See Also==
-	*[[ATP synthase\|ATP synthase]]
	*[[ATPase\|ATPase]]		*[[ATPase\|ATPase]]
	== References ==		== References ==
Line 26:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Methanosarcina mazei]]		[[Category: Methanosarcina mazei]]
-	[[Category: Gruber, G.]]	+	[[Category: Gruber, G]]
-	[[Category: Jeyakanthan, J.]]	+	[[Category: Jeyakanthan, J]]
-	[[Category: Manimekalai, M S.S.]]	+	[[Category: Manimekalai, M S.S]]
-	[[Category: Tadwal, V S.]]	+	[[Category: Tadwal, V S]]
	[[Category: Atp synthesis]]		[[Category: Atp synthesis]]
	[[Category: Hydrogen ion transport]]		[[Category: Hydrogen ion transport]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:20, 11 June 2014

OCA — Wed, 11 Jun 2014 09:20:55 GMT

←Older revision		Revision as of 09:20, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tiv.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of subunit B mutant N157A of the A1AO ATP synthase==
		+	<StructureSection load='3tiv' size='340' side='right' caption='[[3tiv]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TIV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c61\|2c61]], [[3dsr\|3dsr]], [[3eiu\|3eiu]], [[2rkw\|2rkw]], [[3ssa\|3ssa]], [[3tgw\|3tgw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahaB, atpB, MM_0779 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2209 Methanosarcina mazei])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tiv RCSB], [http://www.ebi.ac.uk/pdbsum/3tiv PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A strategically placed tryptophan in position of Arg416 was used as an optical probe to monitor adenosine triphosphate and adenosine-diphosphate binding to subunit B of the A(1)A(O) adenosine triphosphate (ATP) synthase from Methanosarcina mazei Go1. Tryptophan fluorescence and fluorescence correlation spectroscopy gave binding constants indicating a preferred binding of ATP over ADP to the protein. The X-ray crystal structure of the R416W mutant protein in the presence of ATP was solved to 2.1 A resolution, showing the substituted Trp-residue inside the predicted adenine-binding pocket. The cocrystallized ATP molecule could be trapped in a so-called transition nucleotide-binding state. The high resolution structure shows the phosphate residues of the ATP near the P-loop region (S150-E158) and its adenine ring forms pi-pi interaction with Phe149. This transition binding position of ATP could be confirmed by tryptophan emission spectra using the subunit B mutant F149W. The trapped ATP position, similar to the one of the binding region of the antibiotic efrapeptin in F(1)F(O) ATP synthases, is discussed in light of a transition nucleotide-binding state of ATP while on its way to the final binding pocket. Finally, the inhibitory effect of efrapeptin C in ATPase activity of a reconstituted A(3)B(3)- and A(3)B(R416W)(3)-subcomplex, composed of subunit A and the B subunit mutant R416W, of the A(1)A(O) ATP synthase is shown.

-	~~{{STRUCTURE_3tiv\| PDB=3tiv \| SCENE= }}~~	+	Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase.,Kumar A, Manimekalai MS, Balakrishna AM, Hunke C, Weigelt S, Sewald N, Gruber G Proteins. 2009 Jun;75(4):807-19. PMID:19003877<ref>PMID:19003877</ref>

-	~~===Crystal structure of subunit B mutant N157A~~ of the ~~A1AO ATP synthase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_19003877}}~~	+	==See Also==
-		+	*[[ATP synthase\|ATP synthase]]
-	==~~About this Structure~~==	+	*[[ATPase\|ATPase]]
-	[[~~3tiv~~]] ~~is a 2 chain structure with sequence from~~ [~~http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA~~].	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:019003877<~~/~~ref~~><~~ref group="xtra">PMID:019020348</ref><ref group="xtra">PMID:019138746</ref><references group="xtra"~~/>	+	</StructureSection>
	[[Category: Methanosarcina mazei]]		[[Category: Methanosarcina mazei]]
	[[Category: Gruber, G.]]		[[Category: Gruber, G.]]

OCA at 05:30, 5 September 2012

OCA — Wed, 05 Sep 2012 05:30:15 GMT

←Older revision		Revision as of 05:30, 5 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tiv.jpg\|left\|200px]]

-	~~The entry~~ 3tiv ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3tiv\| PDB=3tiv \| SCENE= }}

-	~~Authors: Tadwal, V.S., Manimekalai, M.S.S., Jeyakanthan, J., Gruber, G.~~	+	===Crystal structure of subunit B mutant N157A of the A1AO ATP synthase===

-	~~Description: Crystal~~ structure ~~of subunit B mutant N157A of the A1AO ATP synthase~~	+	{{ABSTRACT_PUBMED_19003877}}
		+
		+	==About this Structure==
		+	[[3tiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIV OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:019003877</ref><ref group="xtra">PMID:019020348</ref><ref group="xtra">PMID:019138746</ref><references group="xtra"/>
		+	[[Category: Methanosarcina mazei]]
		+	[[Category: Gruber, G.]]
		+	[[Category: Jeyakanthan, J.]]
		+	[[Category: Manimekalai, M S.S.]]
		+	[[Category: Tadwal, V S.]]
		+	[[Category: Atp synthesis]]
		+	[[Category: Hydrogen ion transport]]
		+	[[Category: Hydrolase]]

OCA at 05:30, 21 September 2011

OCA — Wed, 21 Sep 2011 05:30:23 GMT

←Older revision		Revision as of 05:30, 21 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tiv is ON HOLD	+	The entry 3tiv is ON HOLD until Paper Publication

	Authors: Tadwal, V.S., Manimekalai, M.S.S., Jeyakanthan, J., Gruber, G.		Authors: Tadwal, V.S., Manimekalai, M.S.S., Jeyakanthan, J., Gruber, G.

	Description: Crystal structure of subunit B mutant N157A of the A1AO ATP synthase		Description: Crystal structure of subunit B mutant N157A of the A1AO ATP synthase

OCA at 05:48, 7 September 2011

OCA — Wed, 07 Sep 2011 05:48:06 GMT

←Older revision		Revision as of 05:48, 7 September 2011
Line 3:		Line 3:
	The entry 3tiv is ON HOLD		The entry 3tiv is ON HOLD

-	Authors: Tadwal, V. S., Manimekalai, M. S. S., Jeyakanthan, J., Gruber, G.	+	Authors: Tadwal, V.S., Manimekalai, M.S.S., Jeyakanthan, J., Gruber, G.

	Description: Crystal structure of subunit B mutant N157A of the A1AO ATP synthase		Description: Crystal structure of subunit B mutant N157A of the A1AO ATP synthase

OCA: Protected "3tiv" [edit=sysop:move=sysop]

OCA — Wed, 31 Aug 2011 06:26:07 GMT

Protected "3tiv" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:26, 31 August 2011

OCA: New page: '''Unreleased structure''' The entry 3tiv is ON HOLD Authors: Tadwal, V. S., Manimekalai, M. S. S., Jeyakanthan, J., Gruber, G. Description: Crystal structure of subunit B mutant N157A...

OCA — Wed, 31 Aug 2011 06:26:06 GMT

New page: '''Unreleased structure''' The entry 3tiv is ON HOLD Authors: Tadwal, V. S., Manimekalai, M. S. S., Jeyakanthan, J., Gruber, G. Description: Crystal structure of subunit B mutant N157A...

New page

'''Unreleased structure'''

The entry 3tiv is ON HOLD

Authors: Tadwal, V. S., Manimekalai, M. S. S., Jeyakanthan, J., Gruber, G.

Description: Crystal structure of subunit B mutant N157A of the A1AO ATP synthase