3tjk - Revision history

OCA at 13:30, 14 March 2024

OCA — Thu, 14 Mar 2024 13:30:58 GMT

←Older revision		Revision as of 13:30, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3tjk' size='340' side='right'caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='3tjk' size='340' side='right'caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJK FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PRDX4 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [https://pdbe.org/3tjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [https://www.ebi.ac.uk/pdbsum/3tjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [https://pdbe.org/3tjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [https://www.ebi.ac.uk/pdbsum/3tjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PRDX4_HUMAN PRDX4_HUMAN]] Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.<ref>PMID:9388242</ref>	+	[https://www.uniprot.org/uniprot/PRDX4_HUMAN PRDX4_HUMAN] Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.<ref>PMID:9388242</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Peroxiredoxin IV (PrxIV) is an endoplasmic reticulum (ER) localized enzyme that metabolizes the hydrogen peroxide produced by ER oxidase 1 (Ero1). It has been shown to play a role in de novo disulfide formation, oxidizing members of the protein disulfide isomerase (PDI) family of enzymes and is a member of the typical 2-cys peroxiredoxin family. We have determined the crystal structure of both reduced and disulfide-bonded, as well as a resolving cysteine mutant of human PrxIV. We show that PrxIV has a similar structure to other typical 2-cys peroxiredoxins and undergoes a conformational change from a fully folded (FF) to a locally unfolded (LU) form following the formation of a disulfide between the peroxidatic and resolving cysteine residues. Unlike other mammalian typical 2-cys peroxiredoxins, we show that PrxIV forms a stable decameric structure even in its disulfide bonded state. In addition, the structure of a resolving cysteine mutant reveals an intermediate in the reaction cycle that adopts the LU conformation. Interestingly the peroxidatic cysteine in the crystal structure is sulfenylated rather than sulfinylated or sulfonylated. In addition, the peroxidatic cysteine in the resolving cysteine mutant is resistant to hyper-oxidation following incubation with high concentration of hydrogen peroxide. These results highlight some unique properties of PrxIV and suggest that the equilibrium between the FF and LU forms favors the LU conformation upon sulfenylation of the peroxidatic cysteine residue.	+
-		+
-	Crystal structure of reduced and of oxidized peroxiredoxin IV reveals a stable oxidized decamer and a non disulfide-bonded intermediate in the catalytic cycle.,Cao Z, Tavender TJ, Roszak AW, Cogdell RJ, Bulleid NJ J Biol Chem. 2011 Oct 12. PMID:21994946<ref>PMID:21994946</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tjk" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 27:		Line 16:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Peroxiredoxin]]~~	+	[[Category: Bulleid NJ]]
-	[[Category: Bulleid~~, N J~~]]	+	[[Category: Cao Z]]
-	[[Category: Cao, Z]]	+	[[Category: Cogdell RJ]]
-	[[Category: Cogdell~~, R J~~]]	+	[[Category: Roszak AW]]
-	[[Category: Roszak~~, A W~~]]	+	[[Category: Tavender TJ]]
-	[[Category: Tavender~~, T J]]~~	+
-	~~[[Category: Endoplasmic reticulum]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Sulfenylation]]~~	+
-	~~[[Category: Thioredoxin fold~~]]	+

OCA at 17:01, 6 July 2022

OCA — Wed, 06 Jul 2022 17:01:04 GMT

←Older revision		Revision as of 17:01, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form==		==Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form==
-	<StructureSection load='3tjk' size='340' side='right' caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>	+	<StructureSection load='3tjk' size='340' side='right'caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TJK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJK FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX4 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [~~http~~://pdbe.org/3tjk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tjk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [https://pdbe.org/3tjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [https://www.ebi.ac.uk/pdbsum/3tjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PRDX4_HUMAN PRDX4_HUMAN]] Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.<ref>PMID:9388242</ref>	+	[[https://www.uniprot.org/uniprot/PRDX4_HUMAN PRDX4_HUMAN]] Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.<ref>PMID:9388242</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Peroxiredoxin\|Peroxiredoxin]]	+	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
	[[Category: Bulleid, N J]]		[[Category: Bulleid, N J]]

OCA at 20:33, 5 August 2016

OCA — Fri, 05 Aug 2016 20:33:13 GMT

←Older revision		Revision as of 20:33, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form==		==Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form==
	<StructureSection load='3tjk' size='340' side='right' caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='3tjk' size='340' side='right' caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [http://www.ebi.ac.uk/pdbsum/3tjk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [http://pdbe.org/3tjk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [http://www.ebi.ac.uk/pdbsum/3tjk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tjk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
	[[Category: Bulleid, N J]]		[[Category: Bulleid, N J]]

OCA at 05:38, 25 December 2014

OCA — Thu, 25 Dec 2014 05:38:19 GMT

←Older revision		Revision as of 05:38, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [http://www.ebi.ac.uk/pdbsum/3tjk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [http://www.ebi.ac.uk/pdbsum/3tjk PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRDX4_HUMAN PRDX4_HUMAN]] Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.<ref>PMID:9388242</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:25, 9 December 2014

OCA — Tue, 09 Dec 2014 17:25:37 GMT

←Older revision		Revision as of 17:25, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3tjk.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form==
		+	<StructureSection load='3tjk' size='340' side='right' caption='[[3tjk]], [[Resolution\|resolution]] 2.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJK FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tjb\|3tjb]], [[3tjf\|3tjf]], [[3tjg\|3tjg]], [[3tjj\|3tjj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PRDX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxiredoxin Peroxiredoxin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.15 1.11.1.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjk RCSB], [http://www.ebi.ac.uk/pdbsum/3tjk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peroxiredoxin IV (PrxIV) is an endoplasmic reticulum (ER) localized enzyme that metabolizes the hydrogen peroxide produced by ER oxidase 1 (Ero1). It has been shown to play a role in de novo disulfide formation, oxidizing members of the protein disulfide isomerase (PDI) family of enzymes and is a member of the typical 2-cys peroxiredoxin family. We have determined the crystal structure of both reduced and disulfide-bonded, as well as a resolving cysteine mutant of human PrxIV. We show that PrxIV has a similar structure to other typical 2-cys peroxiredoxins and undergoes a conformational change from a fully folded (FF) to a locally unfolded (LU) form following the formation of a disulfide between the peroxidatic and resolving cysteine residues. Unlike other mammalian typical 2-cys peroxiredoxins, we show that PrxIV forms a stable decameric structure even in its disulfide bonded state. In addition, the structure of a resolving cysteine mutant reveals an intermediate in the reaction cycle that adopts the LU conformation. Interestingly the peroxidatic cysteine in the crystal structure is sulfenylated rather than sulfinylated or sulfonylated. In addition, the peroxidatic cysteine in the resolving cysteine mutant is resistant to hyper-oxidation following incubation with high concentration of hydrogen peroxide. These results highlight some unique properties of PrxIV and suggest that the equilibrium between the FF and LU forms favors the LU conformation upon sulfenylation of the peroxidatic cysteine residue.

-	~~{{STRUCTURE_3tjk\| PDB=3tjk \| SCENE= }}~~	+	Crystal structure of reduced and of oxidized peroxiredoxin IV reveals a stable oxidized decamer and a non disulfide-bonded intermediate in the catalytic cycle.,Cao Z, Tavender TJ, Roszak AW, Cogdell RJ, Bulleid NJ J Biol Chem. 2011 Oct 12. PMID:21994946<ref>PMID:21994946</ref>

-	~~===Crystal Structure~~ of ~~human peroxiredoxin IV C245A mutant in reduced form===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21994946}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tjk]] is a 5 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].~~	+

	==See Also==		==See Also==
	*[[Peroxiredoxin\|Peroxiredoxin]]		*[[Peroxiredoxin\|Peroxiredoxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021994946<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]
-	[[Category: Bulleid, N J.]]	+	[[Category: Bulleid, N J]]
-	[[Category: Cao, Z.]]	+	[[Category: Cao, Z]]
-	[[Category: Cogdell, R J.]]	+	[[Category: Cogdell, R J]]
-	[[Category: Roszak, A W.]]	+	[[Category: Roszak, A W]]
-	[[Category: Tavender, T J.]]	+	[[Category: Tavender, T J]]
	[[Category: Endoplasmic reticulum]]		[[Category: Endoplasmic reticulum]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Sulfenylation]]		[[Category: Sulfenylation]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]

OCA at 13:14, 20 October 2012

OCA — Sat, 20 Oct 2012 13:14:45 GMT

←Older revision		Revision as of 13:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3tjk]] is a 5 chain structure ~~of [[Peroxiredoxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].	+	[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].

	==See Also==		==See Also==

OCA at 11:20, 26 July 2012

OCA — Thu, 26 Jul 2012 11:20:38 GMT

←Older revision		Revision as of 11:20, 26 July 2012
Line 1:		Line 1:
	[[Image:3tjk.png\|left\|200px]]		[[Image:3tjk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3tjk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3tjk\| PDB=3tjk \| SCENE= }}		{{STRUCTURE_3tjk\| PDB=3tjk \| SCENE= }}

	===Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form===		===Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21994946}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21994946 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21994946}}		{{ABSTRACT_PUBMED_21994946}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Peroxiredoxin]]	+	*[[Peroxiredoxin\|Peroxiredoxin]]

	==Reference==		==Reference==

OCA at 10:51, 14 December 2011

OCA — Wed, 14 Dec 2011 10:51:22 GMT

←Older revision		Revision as of 10:51, 14 December 2011
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].	+	[[3tjk]] is a 5 chain structure of [[Peroxiredoxin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].
		+
		+	==See Also==
		+	*[[Peroxiredoxin]]

	==Reference==		==Reference==

OCA at 10:55, 16 November 2011

OCA — Wed, 16 Nov 2011 10:55:02 GMT

←Older revision		Revision as of 10:55, 16 November 2011
Line 11:		Line 11:
	===Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form===		===Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21994946}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21994946 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21994946}}

	==About this Structure==		==About this Structure==
	[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].		[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021994946</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]

OCA at 09:50, 30 October 2011

OCA — Sun, 30 Oct 2011 09:50:17 GMT

←Older revision		Revision as of 09:50, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tjk.png\|left\|200px]]

-	The ~~entry 3tjk~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tjk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tjk\| PDB=3tjk \| SCENE= }}

-	~~Authors: Cao, Z., Tavender, T.J., Roszak, A.W., Cogdell, R.J., Bulleid, N.J.~~	+	===Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form===

-	~~Description: Crystal~~ Structure ~~of human peroxiredoxin IV C245A mutant in reduced form~~	+
		+	==About this Structure==
		+	[[3tjk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJK OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Peroxiredoxin]]
		+	[[Category: Bulleid, N J.]]
		+	[[Category: Cao, Z.]]
		+	[[Category: Cogdell, R J.]]
		+	[[Category: Roszak, A W.]]
		+	[[Category: Tavender, T J.]]
		+	[[Category: Endoplasmic reticulum]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Sulfenylation]]
		+	[[Category: Thioredoxin fold]]