3tjz - Revision history

OCA at 10:09, 1 March 2024

OCA — Fri, 01 Mar 2024 10:09:39 GMT

←Older revision		Revision as of 10:09, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tjz' size='340' side='right'caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3tjz' size='340' side='right'caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~] and [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~GUANYLATE+ESTER'>GNP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ARF1, YDL192W, D1244 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=559292 Baker~~'~~s yeast])~~, ~~COPG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9913 BOVIN]), COPZ1, COPZ ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [https://pdbe.org/3tjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [https://www.ebi.ac.uk/pdbsum/3tjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [https://pdbe.org/3tjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [https://www.ebi.ac.uk/pdbsum/3tjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[https://www.uniprot.org/uniprot/COPZ1_BOVIN COPZ1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex. [[https://www.uniprot.org/uniprot/COPG1_BOVIN COPG1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).	+	[https://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Budding of COPI-coated vesicles from Golgi membranes requires an Arf family G protein and the coatomer complex recruited from cytosol. Arf is also required with coatomer-related clathrin adaptor complexes to bud vesicles from the trans-Golgi network and endosomal compartments. To understand the structural basis for Arf-dependent recruitment of a vesicular coat to the membrane, we determined the structure of Arf1 bound to the gammazeta-COP subcomplex of coatomer. Structure-guided biochemical analysis reveals that a second Arf1-GTP molecule binds to betadelta-COP at a site common to the gamma- and beta-COP subunits. The Arf1-binding sites on coatomer are spatially related to PtdIns4,5P(2)-binding sites on the endocytic AP2 complex, providing evidence that the orientation of membrane binding is general for this class of vesicular coat proteins. A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules.	+
-		+
-	~~A structure-based mechanism for arf1-dependent recruitment of coatomer to membranes.,Yu X, Breitman M, Goldberg J Cell. 2012 Feb 3;148(3):530-42. PMID:22304919<ref>PMID:22304919</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tjz" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast]]~~	+	[[Category: Bos taurus]]
-	~~[[Category: Bovin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Breitman, M~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Goldberg, J~~]]	+	[[Category: Breitman M]]
-	[[Category: ~~Yu, X~~]]	+	[[Category: Goldberg J]]
-	[[Category: ~~Golgi membrane]]~~	+	[[Category: Yu X]]
-	~~[[Category: Protein trafficking]]~~	+
-	~~[[Category: Protein transport-protein binding complex~~]]	+

OCA at 17:01, 6 July 2022

OCA — Wed, 06 Jul 2022 17:01:37 GMT

←Older revision		Revision as of 17:01, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex==		==Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex==
-	<StructureSection load='3tjz' size='340' side='right' caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='3tjz' size='340' side='right'caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin] and [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin] and [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARF1, YDL192W, D1244 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), COPG ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), COPZ1, COPZ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARF1, YDL192W, D1244 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), COPG ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), COPZ1, COPZ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [~~http~~://pdbe.org/3tjz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tjz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [https://pdbe.org/3tjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [https://www.ebi.ac.uk/pdbsum/3tjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[~~http~~://www.uniprot.org/uniprot/COPZ1_BOVIN COPZ1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex. [[~~http~~://www.uniprot.org/uniprot/COPG1_BOVIN COPG1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).	+	[[https://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[https://www.uniprot.org/uniprot/COPZ1_BOVIN COPZ1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex. [[https://www.uniprot.org/uniprot/COPG1_BOVIN COPG1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Breitman, M]]		[[Category: Breitman, M]]
	[[Category: Goldberg, J]]		[[Category: Goldberg, J]]

OCA at 17:18, 4 August 2016

OCA — Thu, 04 Aug 2016 17:18:31 GMT

←Older revision		Revision as of 17:18, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex==		==Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex==
	<StructureSection load='3tjz' size='340' side='right' caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3tjz' size='340' side='right' caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~] and [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARF1, YDL192W, D1244 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~4932 Saccharomyces cerevisiae~~]), COPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 ~~Bos taurus~~]), COPZ1, COPZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 ~~Bos taurus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARF1, YDL192W, D1244 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), COPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN]), COPZ1, COPZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [http://www.ebi.ac.uk/pdbsum/3tjz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [http://pdbe.org/3tjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [http://www.ebi.ac.uk/pdbsum/3tjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[http://www.uniprot.org/uniprot/~~COPG_BOVIN COPG_BOVIN~~]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).	+	[[http://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[http://www.uniprot.org/uniprot/COPZ1_BOVIN COPZ1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex. [[http://www.uniprot.org/uniprot/COPG1_BOVIN COPG1_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tjz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Baker's yeast]]
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Bovin]]
	[[Category: Breitman, M]]		[[Category: Breitman, M]]
	[[Category: Goldberg, J]]		[[Category: Goldberg, J]]

OCA at 11:20, 25 December 2014

OCA — Thu, 25 Dec 2014 11:20:30 GMT

←Older revision		Revision as of 11:20, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3tjz.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex==
		+	<StructureSection load='3tjz' size='340' side='right' caption='[[3tjz]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TJZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARF1, YDL192W, D1244 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), COPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), COPZ1, COPZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tjz RCSB], [http://www.ebi.ac.uk/pdbsum/3tjz PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARF1_YEAST ARF1_YEAST]] GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.<ref>PMID:15356266</ref> [[http://www.uniprot.org/uniprot/COPG_BOVIN COPG_BOVIN]] The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Budding of COPI-coated vesicles from Golgi membranes requires an Arf family G protein and the coatomer complex recruited from cytosol. Arf is also required with coatomer-related clathrin adaptor complexes to bud vesicles from the trans-Golgi network and endosomal compartments. To understand the structural basis for Arf-dependent recruitment of a vesicular coat to the membrane, we determined the structure of Arf1 bound to the gammazeta-COP subcomplex of coatomer. Structure-guided biochemical analysis reveals that a second Arf1-GTP molecule binds to betadelta-COP at a site common to the gamma- and beta-COP subunits. The Arf1-binding sites on coatomer are spatially related to PtdIns4,5P(2)-binding sites on the endocytic AP2 complex, providing evidence that the orientation of membrane binding is general for this class of vesicular coat proteins. A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules.

-	<!--	+	A structure-based mechanism for arf1-dependent recruitment of coatomer to membranes.,Yu X, Breitman M, Goldberg J Cell. 2012 Feb 3;148(3):530-42. PMID:22304919<ref>PMID:22304919</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3tjz"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3tjz\| PDB=3tjz \| SCENE= }}~~	+

-	~~===Crystal Structure of Arf1 Bound to the gamma~~/~~zeta-COP Core Complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22304919}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22304919 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22304919}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tjz]] is a 6 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022304919<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Breitman, M.]]	+	[[Category: Breitman, M]]
-	[[Category: Goldberg, J.]]	+	[[Category: Goldberg, J]]
-	[[Category: Yu, X.]]	+	[[Category: Yu, X]]
	[[Category: Golgi membrane]]		[[Category: Golgi membrane]]
	[[Category: Protein trafficking]]		[[Category: Protein trafficking]]
	[[Category: Protein transport-protein binding complex]]		[[Category: Protein transport-protein binding complex]]

OCA at 08:48, 29 February 2012

OCA — Wed, 29 Feb 2012 08:48:17 GMT

←Older revision		Revision as of 08:48, 29 February 2012
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-	[[Image:3tjz.~~jpg~~\|left\|200px]]	+	[[Image:3tjz.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex===		===Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22304919}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22304919 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22304919}}

	==About this Structure==		==About this Structure==

OCA at 07:49, 22 February 2012

OCA — Wed, 22 Feb 2012 07:49:54 GMT

←Older revision		Revision as of 07:49, 22 February 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3tjz.jpg\|left\|200px]]

-	The ~~entry 3tjz~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tjz", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tjz\| PDB=3tjz \| SCENE= }}

-	~~Authors: Goldberg, J., Yu, X., Breitman, M.~~	+	===Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex===

-	~~Description: Crystal~~ Structure ~~of Arf1 Bound to the gamma~~/~~zeta~~-~~COP Core Complex~~	+
		+	==About this Structure==
		+	[[3tjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TJZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022304919</ref><references group="xtra"/>
		+	[[Category: Bos taurus]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Breitman, M.]]
		+	[[Category: Goldberg, J.]]
		+	[[Category: Yu, X.]]
		+	[[Category: Golgi membrane]]
		+	[[Category: Protein trafficking]]
		+	[[Category: Protein transport-protein binding complex]]

OCA at 06:09, 5 October 2011

OCA — Wed, 05 Oct 2011 06:09:21 GMT

←Older revision		Revision as of 06:09, 5 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tjz is ON HOLD	+	The entry 3tjz is ON HOLD until Paper Publication

	Authors: Goldberg, J., Yu, X., Breitman, M.		Authors: Goldberg, J., Yu, X., Breitman, M.

-	Description: Crystal Structure of Arf1 Bound to the -COP Core Complex	+	Description: Crystal Structure of Arf1 Bound to the gamma/zeta-COP Core Complex

OCA at 05:48, 7 September 2011

OCA — Wed, 07 Sep 2011 05:48:37 GMT

←Older revision		Revision as of 05:48, 7 September 2011
Line 3:		Line 3:
	The entry 3tjz is ON HOLD		The entry 3tjz is ON HOLD

-	Authors: Goldberg, J, Yu, X, Breitman, M	+	Authors: Goldberg, J., Yu, X., Breitman, M.

	Description: Crystal Structure of Arf1 Bound to the -COP Core Complex		Description: Crystal Structure of Arf1 Bound to the -COP Core Complex

OCA: Protected "3tjz" [edit=sysop:move=sysop]

OCA — Wed, 31 Aug 2011 06:27:15 GMT

Protected "3tjz" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 31 August 2011

OCA: New page: '''Unreleased structure''' The entry 3tjz is ON HOLD Authors: Goldberg, J, Yu, X, Breitman, M Description: Crystal Structure of Arf1 Bound to the -COP Core Complex

OCA — Wed, 31 Aug 2011 06:27:14 GMT

New page: '''Unreleased structure''' The entry 3tjz is ON HOLD Authors: Goldberg, J, Yu, X, Breitman, M Description: Crystal Structure of Arf1 Bound to the -COP Core Complex

New page

'''Unreleased structure'''

The entry 3tjz is ON HOLD

Authors: Goldberg, J, Yu, X, Breitman, M

Description: Crystal Structure of Arf1 Bound to the -COP Core Complex