3tkn - Revision history

OCA at 10:09, 1 March 2024

OCA — Fri, 01 Mar 2024 10:09:40 GMT

←Older revision		Revision as of 10:09, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3tkn' size='340' side='right'caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='3tkn' size='340' side='right'caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TKN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TKN FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>HRB187, J1135, NUP82, YJL061W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4932 ATCC 18824])~~, ~~NUP158, NUP159, RAT7, YIL115C (~~[~~https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824~~]~~), Nup98 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [https://pdbe.org/3tkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [https://www.ebi.ac.uk/pdbsum/3tkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tkn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [https://pdbe.org/3tkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [https://www.ebi.ac.uk/pdbsum/3tkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tkn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[https://www.uniprot.org/uniprot/NUP98_MOUSE NUP98_MOUSE]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[https://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>	+	[https://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The cytoplasmic filament nucleoporins of the nuclear pore complex (NPC) are critically involved in nuclear export and remodeling of mRNA ribonucleoprotein particles and are associated with various human malignancies. Here, we report the crystal structure of the Nup98 C-terminal autoproteolytic domain, frequently missing from leukemogenic forms of the protein, in complex with the N-terminal domain of Nup82 and the C-terminal tail fragment of Nup159. The Nup82 beta propeller serves as a noncooperative binding platform for both binding partners. Interaction of Nup98 with Nup82 occurs through a reciprocal exchange of loop structures. Strikingly, the same Nup98 groove promiscuously interacts with Nup82 and Nup96 in a mutually excusive fashion. Simultaneous disruption of both Nup82 interactions in yeast causes severe defects in mRNA export, while the severing of a single interaction is tolerated. Thus, the cytoplasmic filament network of the NPC is robust, consistent with its essential function in nucleocytoplasmic transport.	+
-		+
-	Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex.,Stuwe TT, von Borzyskowski LS, Davenport AM, Hoelz A J Mol Biol. 2012 Apr 2. PMID:22480613<ref>PMID:22480613</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tkn" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 25:		Line 16:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Hoelz, A~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Stuwe, T T~~]]	+	[[Category: Hoelz A]]
-	[[Category: ~~Oncoprotein]]~~	+	[[Category: Stuwe TT]]
-	~~[[Category: Protein complex]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 17:02, 6 July 2022

OCA — Wed, 06 Jul 2022 17:02:25 GMT

←Older revision		Revision as of 17:02, 6 July 2022
Line 1:		Line 1:

	==Structure of the Nup82-Nup159-Nup98 heterotrimer==		==Structure of the Nup82-Nup159-Nup98 heterotrimer==
-	<StructureSection load='3tkn' size='340' side='right' caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='3tkn' size='340' side='right'caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TKN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TKN FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), NUP158, NUP159, RAT7, YIL115C ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Nup98 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), NUP158, NUP159, RAT7, YIL115C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Nup98 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [~~http~~://pdbe.org/3tkn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tkn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tkn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [https://pdbe.org/3tkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [https://www.ebi.ac.uk/pdbsum/3tkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tkn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[~~http~~://www.uniprot.org/uniprot/NUP98_MOUSE NUP98_MOUSE]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[~~http~~://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>	+	[[https://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[https://www.uniprot.org/uniprot/NUP98_MOUSE NUP98_MOUSE]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[https://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Nucleoporin\|Nucleoporin]]	+	*[[Nucleoporin 3D structures\|Nucleoporin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Hoelz, A]]		[[Category: Hoelz, A]]

OCA at 12:08, 5 August 2016

OCA — Fri, 05 Aug 2016 12:08:36 GMT

←Older revision		Revision as of 12:08, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of the Nup82-Nup159-Nup98 heterotrimer==		==Structure of the Nup82-Nup159-Nup98 heterotrimer==
	<StructureSection load='3tkn' size='340' side='right' caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='3tkn' size='340' side='right' caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~] and [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TKN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TKN FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~]), NUP158, NUP159, RAT7, YIL115C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~]), Nup98 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), NUP158, NUP159, RAT7, YIL115C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Nup98 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [http://www.ebi.ac.uk/pdbsum/3tkn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [http://pdbe.org/3tkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [http://www.ebi.ac.uk/pdbsum/3tkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tkn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[http://www.uniprot.org/uniprot/~~Q6PFD9_MOUSE Q6PFD9_MOUSE~~]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[http://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>	+	[[http://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[http://www.uniprot.org/uniprot/NUP98_MOUSE NUP98_MOUSE]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[http://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tkn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Atcc 18824]]
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Hoelz, A]]		[[Category: Hoelz, A]]
	[[Category: Stuwe, T T]]		[[Category: Stuwe, T T]]

OCA at 18:42, 25 December 2014

OCA — Thu, 25 Dec 2014 18:42:26 GMT

←Older revision		Revision as of 18:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TKN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TKN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), NUP158, NUP159, RAT7, YIL115C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Nup98 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), NUP158, NUP159, RAT7, YIL115C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Nup98 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [http://www.ebi.ac.uk/pdbsum/3tkn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [http://www.ebi.ac.uk/pdbsum/3tkn PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NUP82_YEAST NUP82_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.<ref>PMID:7559750</ref> <ref>PMID:9843582</ref> <ref>PMID:10801828</ref> <ref>PMID:10891509</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> [[http://www.uniprot.org/uniprot/Q6PFD9_MOUSE Q6PFD9_MOUSE]] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC (By similarity). [[http://www.uniprot.org/uniprot/NU159_YEAST NU159_YEAST]] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.<ref>PMID:9736720</ref> <ref>PMID:9843582</ref> <ref>PMID:9891088</ref> <ref>PMID:10523319</ref> <ref>PMID:10801828</ref> <ref>PMID:10952996</ref> <ref>PMID:11387327</ref> <ref>PMID:11739405</ref> <ref>PMID:11689687</ref> <ref>PMID:12543930</ref> <ref>PMID:12604785</ref> <ref>PMID:15039779</ref> <ref>PMID:15574330</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 12:		Line 14:
	Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex.,Stuwe TT, von Borzyskowski LS, Davenport AM, Hoelz A J Mol Biol. 2012 Apr 2. PMID:22480613<ref>PMID:22480613</ref>		Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex.,Stuwe TT, von Borzyskowski LS, Davenport AM, Hoelz A J Mol Biol. 2012 Apr 2. PMID:22480613<ref>PMID:22480613</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 23:		Line 25:
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hoelz, A.]]	+	[[Category: Hoelz, A]]
-	[[Category: Stuwe, T T.]]	+	[[Category: Stuwe, T T]]
	[[Category: Oncoprotein]]		[[Category: Oncoprotein]]
	[[Category: Protein complex]]		[[Category: Protein complex]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 06:04, 5 June 2014

OCA — Thu, 05 Jun 2014 06:04:18 GMT

←Older revision		Revision as of 06:04, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tkn.~~png~~\|~~left\|200px~~]]	+	==Structure of the Nup82-Nup159-Nup98 heterotrimer==
		+	<StructureSection load='3tkn' size='340' side='right' caption='[[3tkn]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TKN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HRB187, J1135, NUP82, YJL061W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), NUP158, NUP159, RAT7, YIL115C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Nup98 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tkn RCSB], [http://www.ebi.ac.uk/pdbsum/3tkn PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cytoplasmic filament nucleoporins of the nuclear pore complex (NPC) are critically involved in nuclear export and remodeling of mRNA ribonucleoprotein particles and are associated with various human malignancies. Here, we report the crystal structure of the Nup98 C-terminal autoproteolytic domain, frequently missing from leukemogenic forms of the protein, in complex with the N-terminal domain of Nup82 and the C-terminal tail fragment of Nup159. The Nup82 beta propeller serves as a noncooperative binding platform for both binding partners. Interaction of Nup98 with Nup82 occurs through a reciprocal exchange of loop structures. Strikingly, the same Nup98 groove promiscuously interacts with Nup82 and Nup96 in a mutually excusive fashion. Simultaneous disruption of both Nup82 interactions in yeast causes severe defects in mRNA export, while the severing of a single interaction is tolerated. Thus, the cytoplasmic filament network of the NPC is robust, consistent with its essential function in nucleocytoplasmic transport.

-	<!--	+	Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex.,Stuwe TT, von Borzyskowski LS, Davenport AM, Hoelz A J Mol Biol. 2012 Apr 2. PMID:22480613<ref>PMID:22480613</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3tkn", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3tkn\| PDB=3tkn \| SCENE= }}~~	+

-	~~===Structure~~ of the ~~Nup82-Nup159-Nup98 heterotrimer===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Nucleoporin\|Nucleoporin]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22480613}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22480613 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22480613}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3tkn~~]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022480613<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 06:56, 18 April 2012

OCA — Wed, 18 Apr 2012 06:56:29 GMT

←Older revision		Revision as of 06:56, 18 April 2012
Line 1:		Line 1:
-	[[Image:3tkn.~~jpg~~\|left\|200px]]	+	[[Image:3tkn.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Structure of the Nup82-Nup159-Nup98 heterotrimer===		===Structure of the Nup82-Nup159-Nup98 heterotrimer===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22480613}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22480613 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22480613}}

	==About this Structure==		==About this Structure==
	[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA].		[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022480613</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 07:09, 11 April 2012

OCA — Wed, 11 Apr 2012 07:09:03 GMT

←Older revision		Revision as of 07:09, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tkn.jpg\|left\|200px]]

-	The ~~entry 3tkn~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tkn", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tkn\| PDB=3tkn \| SCENE= }}

-	~~Authors: Stuwe, T.T., Hoelz, A.~~	+	===Structure of the Nup82-Nup159-Nup98 heterotrimer===

-	~~Description:~~ Structure of a ~~multimeric nucleoporin~~ complex	+
		+	==About this Structure==
		+	[[3tkn]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TKN OCA].
		+	[[Category: Mus musculus]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Hoelz, A.]]
		+	[[Category: Stuwe, T T.]]
		+	[[Category: Oncoprotein]]
		+	[[Category: Protein complex]]
		+	[[Category: Protein transport]]

OCA at 08:40, 30 October 2011

OCA — Sun, 30 Oct 2011 08:40:23 GMT

←Older revision		Revision as of 08:40, 30 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tkn is ON HOLD	+	The entry 3tkn is ON HOLD until Paper Publication

	Authors: Stuwe, T.T., Hoelz, A.		Authors: Stuwe, T.T., Hoelz, A.

	Description: Structure of a multimeric nucleoporin complex		Description: Structure of a multimeric nucleoporin complex

OCA: Protected "3tkn" [edit=sysop:move=sysop]

OCA — Wed, 07 Sep 2011 05:49:08 GMT

Protected "3tkn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 7 September 2011

OCA: New page: '''Unreleased structure''' The entry 3tkn is ON HOLD Authors: Stuwe, T.T., Hoelz, A. Description: Structure of a multimeric nucleoporin complex

OCA — Wed, 07 Sep 2011 05:49:07 GMT

New page: '''Unreleased structure''' The entry 3tkn is ON HOLD Authors: Stuwe, T.T., Hoelz, A. Description: Structure of a multimeric nucleoporin complex

New page

'''Unreleased structure'''

The entry 3tkn is ON HOLD

Authors: Stuwe, T.T., Hoelz, A.

Description: Structure of a multimeric nucleoporin complex