3tly - Revision history

OCA at 13:33, 14 March 2024

OCA — Thu, 14 Mar 2024 13:33:18 GMT

←Older revision		Revision as of 13:33, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3tly' size='340' side='right'caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3tly' size='340' side='right'caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [https://pdbe.org/3tly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [https://www.ebi.ac.uk/pdbsum/3tly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tly ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [https://pdbe.org/3tly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [https://www.ebi.ac.uk/pdbsum/3tly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tly ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/MCCF_ECOLX MCCF_ECOLX] Involved in specific self-immunity to microcin C7.
-	~~Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets~~. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. Subsequent proteolytic processing releases the toxic compound inside the cell. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in ~~complex with various ligands. The MccF structure is similar to that of dipeptide ld~~-~~carboxypeptidase, but with an additional loop proximal~~ to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.	+
-		+
-	~~Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic~~ microcin C7.~~,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3tly" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agarwal, V]]	+	[[Category: Agarwal V]]
-	[[Category: Nair~~, S K]]~~	+	[[Category: Nair SK]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 17:03, 6 July 2022

OCA — Wed, 06 Jul 2022 17:03:44 GMT

←Older revision		Revision as of 17:03, 6 July 2022
Line 1:		Line 1:

	==Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state==		==Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state==
-	<StructureSection load='3tly' size='340' side='right' caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3tly' size='340' side='right'caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TLY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TLY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [~~http~~://pdbe.org/3tly PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tly PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tly ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [https://pdbe.org/3tly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [https://www.ebi.ac.uk/pdbsum/3tly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tly ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 08:29, 11 August 2016

OCA — Thu, 11 Aug 2016 08:29:58 GMT

←Older revision		Revision as of 08:29, 11 August 2016
Line 1:		Line 1:
		+
	==Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state==		==Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state==
	<StructureSection load='3tly' size='340' side='right' caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3tly' size='340' side='right' caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [http://www.ebi.ac.uk/pdbsum/3tly PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [http://pdbe.org/3tly PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [http://www.ebi.ac.uk/pdbsum/3tly PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tly ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tly" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Nair, S K]]		[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 10:39, 4 January 2015

OCA — Sun, 04 Jan 2015 10:39:34 GMT

←Older revision		Revision as of 10:39, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [http://www.ebi.ac.uk/pdbsum/3tly PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [http://www.ebi.ac.uk/pdbsum/3tly PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>		Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Agarwal, V.]]	+	[[Category: Agarwal, V]]
-	[[Category: Nair, S K.]]	+	[[Category: Nair, S K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 06:13, 5 June 2014

OCA — Thu, 05 Jun 2014 06:13:51 GMT

←Older revision		Revision as of 06:13, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3tly.~~png~~\|~~left~~\|~~200px~~]]	+	==Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state==
		+	<StructureSection load='3tly' size='340' side='right' caption='[[3tly]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tla\|3tla]], [[3tlb\|3tlb]], [[3tlc\|3tlc]], [[3tle\|3tle]], [[3tlg\|3tlg]], [[3tlz\|3tlz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mccF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tly OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tly RCSB], [http://www.ebi.ac.uk/pdbsum/3tly PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Several classes of naturally occurring antimicrobials exert their antibiotic activity by specifically targeting aminoacyl-tRNA synthetases, validating these enzymes as drug targets. The aspartyl tRNA synthetase "Trojan horse" inhibitor microcin C7 (McC7) consists of a nonhydrolyzable aspartyl-adenylate conjugated to a hexapeptide carrier that facilitates active import into bacterial cells through an oligopeptide transport system. Subsequent proteolytic processing releases the toxic compound inside the cell. Producing strains of McC7 must protect themselves against autotoxicity that may result from premature processing. The mccF gene confers resistance against endogenous and exogenous McC7 by hydrolyzing the amide bond that connects the peptide and nucleotide moieties of McC7. We present here crystal structures of MccF, in complex with various ligands. The MccF structure is similar to that of dipeptide ld-carboxypeptidase, but with an additional loop proximal to the active site that serves as the primary determinant for recognition of adenylated substrates. Wild-type MccF only hydrolyzes the naturally occurring aspartyl phosphoramidate McC7 and synthetic peptidyl sulfamoyl adenylates that contain anionic side chains. We show that substitutions of two active site MccF residues result in a specificity switch toward aromatic aminoacyl-adenylate substrates. These results suggest how MccF-like enzymes may be used to avert various toxic aminoacyl-adenylates that accumulate during antibiotic biosynthesis or in normal metabolism of the cell.

-	~~<!--~~	+	Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.,Agarwal V, Tikhonov A, Metlitskaya A, Severinov K, Nair SK Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4425-30. Epub 2012 Mar 2. PMID:22388748<ref>PMID:22388748</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3tly", creates the "~~Structure ~~Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3tly\| PDB=3tly \| SCENE= }}~~	+

-	~~===Microcin C7 self immunity protein MccF active site mutant S118A~~/~~N220A/K247A in the apo state===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22388748}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22388748 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22388748}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3tly]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022388748<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 07:03, 28 March 2012

OCA — Wed, 28 Mar 2012 07:03:15 GMT

←Older revision		Revision as of 07:03, 28 March 2012
Line 1:		Line 1:
-	[[Image:3tly.~~jpg~~\|left\|200px]]	+	[[Image:3tly.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state===		===Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22388748}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22388748 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22388748}}

	==About this Structure==		==About this Structure==
	[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA].		[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022388748</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 08:59, 29 February 2012

OCA — Wed, 29 Feb 2012 08:59:57 GMT

←Older revision		Revision as of 08:59, 29 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tly.jpg\|left\|200px]]

-	The ~~entry 3tly~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tly", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tly\| PDB=3tly \| SCENE= }}

-	~~Authors: Agarwal, V., Nair, S.K.~~	+	===Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state===

-	~~Description~~: ~~Microcin C7 self immunity protein MccF active site mutant S118A~~/~~N220A~~/~~K247A in the apo state~~	+
		+	==About this Structure==
		+	[[3tly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLY OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Agarwal, V.]]
		+	[[Category: Nair, S K.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Serine protease]]

OCA at 05:31, 21 September 2011

OCA — Wed, 21 Sep 2011 05:31:06 GMT

←Older revision		Revision as of 05:31, 21 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3tly is ON HOLD	+	The entry 3tly is ON HOLD until Paper Publication

	Authors: Agarwal, V., Nair, S.K.		Authors: Agarwal, V., Nair, S.K.

	Description: Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state		Description: Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state

OCA: Protected "3tly" [edit=sysop:move=sysop]

OCA — Wed, 07 Sep 2011 05:50:21 GMT

Protected "3tly" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 7 September 2011

OCA: New page: '''Unreleased structure''' The entry 3tly is ON HOLD Authors: Agarwal, V., Nair, S.K. Description: Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the ap...

OCA — Wed, 07 Sep 2011 05:50:20 GMT

New page: '''Unreleased structure''' The entry 3tly is ON HOLD Authors: Agarwal, V., Nair, S.K. Description: Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the ap...

New page

'''Unreleased structure'''

The entry 3tly is ON HOLD

Authors: Agarwal, V., Nair, S.K.

Description: Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state