3to0 - Revision history

OCA at 13:34, 14 March 2024

OCA — Thu, 14 Mar 2024 13:34:37 GMT

←Older revision		Revision as of 13:34, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3to0' size='340' side='right'caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>		<StructureSection load='3to0' size='340' side='right'caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.655Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~IYD-1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=10090 LK3 transgenic mice])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [https://pdbe.org/3to0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [https://www.ebi.ac.uk/pdbsum/3to0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3to0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [https://pdbe.org/3to0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [https://www.ebi.ac.uk/pdbsum/3to0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3to0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IYD1_MOUSE IYD1_MOUSE]] Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine (By similarity).	+	[https://www.uniprot.org/uniprot/IYD1_MOUSE IYD1_MOUSE] Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Reductive deiodination is critical for thyroid function and represents an unusual exception to the more common oxidative and hydrolytic mechanisms of dehalogenation in mammals. Studies on the reductive processes have been limited by a lack of convenient methods for heterologous expression of the appropriate proteins in large scale. The enzyme responsible for iodide salvage in the thyroid, iodotyrosine deodinase, is now readily generated after engineering its gene from Mus musculus. High expression of a truncated derivative lacking the membrane domain at its N-terminal was observed in Sf9 cells whereas expression in Pichia pastoris remained low despite codon optimization. Ultimately, the desired expression in Escherichia coli was achieved after replacing the two conserved Cys residues of the deiodinase with Ala and fusing the resulting protein to thioredoxin. This final construct provided abundant enzyme for crystallography and mutagenesis. Utility of the E. coli system was demonstrated by examining a set of active site residues critical for binding to the zwitterionic portion of substrate.	+
-		+
-	Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from mus musculus.,Buss JM, McTamney PM, Rokita TE Protein Sci. 2012 Jan 11. doi: 10.1002/pro.2020. PMID:22238141<ref>PMID:22238141</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3to0" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]		*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Iodotyrosine deiodinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Buss~~, J M~~]]	+	[[Category: Buss JM]]
-	[[Category: McTamney~~, P M~~]]	+	[[Category: McTamney PM]]
-	[[Category: Rokita~~, S E]]~~	+	[[Category: Rokita SE]]
-	~~[[Category: Dehalogenase]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Iodide salvage]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Mono-iodotyrosine]]~~	+
-	~~[[Category: Nadp]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Transmembrane~~]]	+

OCA at 17:06, 6 July 2022

OCA — Wed, 06 Jul 2022 17:06:01 GMT

←Older revision		Revision as of 17:06, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN==		==Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN==
-	<StructureSection load='3to0' size='340' side='right' caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>	+	<StructureSection load='3to0' size='340' side='right'caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [~~http~~://pdbe.org/3to0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3to0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3to0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [https://pdbe.org/3to0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [https://www.ebi.ac.uk/pdbsum/3to0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3to0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IYD1_MOUSE IYD1_MOUSE]] Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine (By similarity).	+	[[https://www.uniprot.org/uniprot/IYD1_MOUSE IYD1_MOUSE]] Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Dehalogenase\|Dehalogenase]]	+	*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Iodotyrosine deiodinase]]		[[Category: Iodotyrosine deiodinase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Buss, J M]]		[[Category: Buss, J M]]

OCA at 02:38, 5 August 2016

OCA — Fri, 05 Aug 2016 02:38:07 GMT

←Older revision		Revision as of 02:38, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN==		==Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN==
	<StructureSection load='3to0' size='340' side='right' caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>		<StructureSection load='3to0' size='340' side='right' caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [http://www.ebi.ac.uk/pdbsum/3to0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [http://pdbe.org/3to0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [http://www.ebi.ac.uk/pdbsum/3to0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3to0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3to0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Iodotyrosine deiodinase]]		[[Category: Iodotyrosine deiodinase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Buss, J M]]		[[Category: Buss, J M]]
	[[Category: McTamney, P M]]		[[Category: McTamney, P M]]

OCA at 15:10, 25 December 2014

OCA — Thu, 25 Dec 2014 15:10:36 GMT

←Older revision		Revision as of 15:10, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [http://www.ebi.ac.uk/pdbsum/3to0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [http://www.ebi.ac.uk/pdbsum/3to0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IYD1_MOUSE IYD1_MOUSE]] Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from mus musculus.,Buss JM, McTamney PM, Rokita TE Protein Sci. 2012 Jan 11. doi: 10.1002/pro.2020. PMID:22238141<ref>PMID:22238141</ref>		Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from mus musculus.,Buss JM, McTamney PM, Rokita TE Protein Sci. 2012 Jan 11. doi: 10.1002/pro.2020. PMID:22238141<ref>PMID:22238141</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Dehalogenase\|Dehalogenase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	[[Category: Iodotyrosine deiodinase]]		[[Category: Iodotyrosine deiodinase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Buss, J M.]]	+	[[Category: Buss, J M]]
-	[[Category: McTamney, P M.]]	+	[[Category: McTamney, P M]]
-	[[Category: Rokita, S E.]]	+	[[Category: Rokita, S E]]
	[[Category: Dehalogenase]]		[[Category: Dehalogenase]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]

OCA at 05:42, 5 June 2014

OCA — Thu, 05 Jun 2014 05:42:37 GMT

←Older revision		Revision as of 05:42, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3to0.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN==
		+	<StructureSection load='3to0' size='340' side='right' caption='[[3to0]], [[Resolution\|resolution]] 2.66Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TO0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gb5\|3gb5]], [[3gfd\|3gfd]], [[3gh8\|3gh8]], [[3tnz\|3tnz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IYD-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Iodotyrosine_deiodinase Iodotyrosine deiodinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.22.1.1 1.22.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3to0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3to0 RCSB], [http://www.ebi.ac.uk/pdbsum/3to0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Reductive deiodination is critical for thyroid function and represents an unusual exception to the more common oxidative and hydrolytic mechanisms of dehalogenation in mammals. Studies on the reductive processes have been limited by a lack of convenient methods for heterologous expression of the appropriate proteins in large scale. The enzyme responsible for iodide salvage in the thyroid, iodotyrosine deodinase, is now readily generated after engineering its gene from Mus musculus. High expression of a truncated derivative lacking the membrane domain at its N-terminal was observed in Sf9 cells whereas expression in Pichia pastoris remained low despite codon optimization. Ultimately, the desired expression in Escherichia coli was achieved after replacing the two conserved Cys residues of the deiodinase with Ala and fusing the resulting protein to thioredoxin. This final construct provided abundant enzyme for crystallography and mutagenesis. Utility of the E. coli system was demonstrated by examining a set of active site residues critical for binding to the zwitterionic portion of substrate.

-	~~<!--~~	+	Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from mus musculus.,Buss JM, McTamney PM, Rokita TE Protein Sci. 2012 Jan 11. doi: 10.1002/pro.2020. PMID:22238141<ref>PMID:22238141</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3to0", creates~~ the ~~"Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3to0\| PDB=3to0 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22238141}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22238141 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22238141}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3to0]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022238141<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Iodotyrosine deiodinase]]		[[Category: Iodotyrosine deiodinase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 05:10, 4 April 2012

OCA — Wed, 04 Apr 2012 05:10:17 GMT

←Older revision		Revision as of 05:10, 4 April 2012
Line 1:		Line 1:
-	[[Image:3to0.~~jpg~~\|left\|200px]]	+	[[Image:3to0.png\|left\|200px]]

	<!--		<!--

OCA at 08:45, 29 February 2012

OCA — Wed, 29 Feb 2012 08:45:07 GMT

←Older revision		Revision as of 08:45, 29 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3to0.jpg\|left\|200px]]

-	The ~~entry 3to0~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3to0", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3to0\| PDB=3to0 \| SCENE= }}

-	~~Authors: Buss~~, ~~J.M., McTamney, P.M., Rokita, S.E.~~	+	===Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN===

-	~~Description~~: ~~Crystal~~ structure of Mus musculus ~~iodotyrosine~~ deiodinase ~~(IYD) C217A~~, ~~C239A bound to FMN~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22238141}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22238141 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22238141}}
		+
		+	==About this Structure==
		+	[[3to0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TO0 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022238141</ref><references group="xtra"/>
		+	[[Category: Iodotyrosine deiodinase]]
		+	[[Category: Mus musculus]]
		+	[[Category: Buss, J M.]]
		+	[[Category: McTamney, P M.]]
		+	[[Category: Rokita, S E.]]
		+	[[Category: Dehalogenase]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Iodide salvage]]
		+	[[Category: Membrane]]
		+	[[Category: Mono-iodotyrosine]]
		+	[[Category: Nadp]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Transmembrane]]

OCA at 11:47, 16 November 2011

OCA — Wed, 16 Nov 2011 11:47:39 GMT

←Older revision		Revision as of 11:47, 16 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3to0 is ON HOLD	+	The entry 3to0 is ON HOLD until Paper Publication

	Authors: Buss, J.M., McTamney, P.M., Rokita, S.E.		Authors: Buss, J.M., McTamney, P.M., Rokita, S.E.

	Description: Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN		Description: Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN

OCA: Protected "3to0" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2011 06:05:47 GMT

Protected "3to0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:05, 14 September 2011

OCA: New page: '''Unreleased structure''' The entry 3to0 is ON HOLD Authors: Buss, J.M., McTamney, P.M., Rokita, S.E. Description: Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217...

OCA — Wed, 14 Sep 2011 06:05:46 GMT

New page: '''Unreleased structure''' The entry 3to0 is ON HOLD Authors: Buss, J.M., McTamney, P.M., Rokita, S.E. Description: Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217...

New page

'''Unreleased structure'''

The entry 3to0 is ON HOLD

Authors: Buss, J.M., McTamney, P.M., Rokita, S.E.

Description: Crystal structure of Mus musculus iodotyrosine deiodinase (IYD) C217A, C239A bound to FMN