3toi - Revision history

OCA at 13:35, 14 March 2024

OCA — Thu, 14 Mar 2024 13:35:07 GMT

←Older revision		Revision as of 13:35, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3toi' size='340' side='right'caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3toi' size='340' side='right'caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TOI FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bla, blaTEM-116 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~>~~</tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta~~-~~lactamase Beta-lactamase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [https://pdbe.org/3toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [https://www.ebi.ac.uk/pdbsum/3toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3toi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [https://pdbe.org/3toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [https://www.ebi.ac.uk/pdbsum/3toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3toi ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
-	~~The stability of proteins is paramount for their therapeutic and industrial use, and thus, is a major task for protein engineering~~. Several types of chemical and physical stabilities are desired and discussion revolves around whether each stability trait needs to be addressed separately and how specific and compatible stabilizing mutations act. ~~We demonstrate a step-wise perturbation-compensation strategy, which identifies mutations rescuing activity of a truncated~~ TEM ~~beta~~-~~lactamase. Analyses relating structural stress with~~ the ~~external stresses of heat, denaturants, and proteases, reveal our second~~-~~site suppressors as general stability centers that also improve~~ the ~~full~~-~~length enzyme. A library of lactamase variants truncated by 15 N~~-~~terminal and 3 C-terminal residues (Bla-NDelta15CDelta3) was subjected~~ to ~~activity selection~~ and ~~DNA shuffling~~. ~~The resulting clone with the best in vivo performance harbored eight mutations, surpassed the full~~-~~length wild-type protein by 5.~~3 ~~degrees C in Tm, displayed significantly higher catalytic activity at elevated temperatures,~~ and ~~showed delayed guanidine~~-~~induced denaturation. The crystal structure~~ of ~~this mutant was solved~~ and ~~provided insights into its stability determinants~~. ~~Stepwise reconstitution~~ of ~~the N- and C-terminus increased its thermal, denaturant and proteolytic resistance successively, leading to a full-length enzyme with a Tm increased by 15~~.~~3 degrees C and a half~~-~~denaturation concentration shifted from 0.53 to 1.75 M guanidinium relative to that~~ of ~~the wild type~~. ~~These improvements demonstrate that iterative truncation~~-~~optimization cycles can exploit stability~~-~~trait linkages in proteins and are exceptionally suited for the creation of progressively stabilized variants and~~/~~or down~~-~~sized proteins without the need for detailed structural or mechanistic information.~~	+
-		+
-	~~Exploring Molecular Linkage of Protein Stability Traits for Enzyme Optimization by Iterative Truncation~~ and ~~Evolution.,Speck J, Hecky J, Tam HK, Arndt KM, Einsle O, Muller KM Biochemistry. 2012 Apr 30. PMID:22545913<ref>PMID:22545913<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.~~S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe~~-~~citations 3toi" style="background~~-~~color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]		*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Einsle, O]]	+	[[Category: Einsle O]]
-	[[Category: Tam~~, H K]]~~	+	[[Category: Tam HK]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 17:06, 6 July 2022

OCA — Wed, 06 Jul 2022 17:06:40 GMT

←Older revision		Revision as of 17:06, 6 July 2022
Line 1:		Line 1:

	==Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization==		==Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization==
-	<StructureSection load='3toi' size='340' side='right' caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3toi' size='340' side='right'caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TOI FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaTEM-116 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaTEM-116 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [~~http~~://pdbe.org/3toi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3toi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3toi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [https://pdbe.org/3toi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [https://www.ebi.ac.uk/pdbsum/3toi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3toi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Einsle, O]]		[[Category: Einsle, O]]
	[[Category: Tam, H K]]		[[Category: Tam, H K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 18:55, 10 December 2016

OCA — Sat, 10 Dec 2016 18:55:26 GMT

←Older revision		Revision as of 18:55, 10 December 2016
Line 1:		Line 1:
		+
	==Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization==		==Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization==
	<StructureSection load='3toi' size='340' side='right' caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3toi' size='340' side='right' caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TOI FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaTEM-116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaTEM-116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [http://www.ebi.ac.uk/pdbsum/3toi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [http://pdbe.org/3toi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [http://www.ebi.ac.uk/pdbsum/3toi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3toi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3toi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Escherichia coli]]
	[[Category: Einsle, O]]		[[Category: Einsle, O]]
	[[Category: Tam, H K]]		[[Category: Tam, H K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 17:11, 9 December 2014

OCA — Tue, 09 Dec 2014 17:11:55 GMT

←Older revision		Revision as of 17:11, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3toi.~~png~~\|~~left\|200px~~]]	+	==Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization==
		+	<StructureSection load='3toi' size='340' side='right' caption='[[3toi]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TOI FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla, blaTEM-116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3toi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3toi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3toi RCSB], [http://www.ebi.ac.uk/pdbsum/3toi PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stability of proteins is paramount for their therapeutic and industrial use, and thus, is a major task for protein engineering. Several types of chemical and physical stabilities are desired and discussion revolves around whether each stability trait needs to be addressed separately and how specific and compatible stabilizing mutations act. We demonstrate a step-wise perturbation-compensation strategy, which identifies mutations rescuing activity of a truncated TEM beta-lactamase. Analyses relating structural stress with the external stresses of heat, denaturants, and proteases, reveal our second-site suppressors as general stability centers that also improve the full-length enzyme. A library of lactamase variants truncated by 15 N-terminal and 3 C-terminal residues (Bla-NDelta15CDelta3) was subjected to activity selection and DNA shuffling. The resulting clone with the best in vivo performance harbored eight mutations, surpassed the full-length wild-type protein by 5.3 degrees C in Tm, displayed significantly higher catalytic activity at elevated temperatures, and showed delayed guanidine-induced denaturation. The crystal structure of this mutant was solved and provided insights into its stability determinants. Stepwise reconstitution of the N- and C-terminus increased its thermal, denaturant and proteolytic resistance successively, leading to a full-length enzyme with a Tm increased by 15.3 degrees C and a half-denaturation concentration shifted from 0.53 to 1.75 M guanidinium relative to that of the wild type. These improvements demonstrate that iterative truncation-optimization cycles can exploit stability-trait linkages in proteins and are exceptionally suited for the creation of progressively stabilized variants and/or down-sized proteins without the need for detailed structural or mechanistic information.

-	~~{{STRUCTURE_3toi\| PDB=3toi \| SCENE= }}~~	+	Exploring Molecular Linkage of Protein Stability Traits for Enzyme Optimization by Iterative Truncation and Evolution.,Speck J, Hecky J, Tam HK, Arndt KM, Einsle O, Muller KM Biochemistry. 2012 Apr 30. PMID:22545913<ref>PMID:22545913</ref>

-	~~===Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22545913}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3toi]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022545913<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Einsle, O.]]	+	[[Category: Einsle, O]]
-	[[Category: Tam, H K.]]	+	[[Category: Tam, H K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 21:22, 20 October 2012

OCA — Sat, 20 Oct 2012 21:22:05 GMT

←Older revision		Revision as of 21:22, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3toi]] is a 2 chain structure ~~of [[Beta-lactamase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].	+	[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].

	==See Also==		==See Also==

OCA at 13:02, 27 July 2012

OCA — Fri, 27 Jul 2012 13:02:11 GMT

←Older revision		Revision as of 13:02, 27 July 2012
Line 1:		Line 1:
-	[[Image:3toi.~~jpg~~\|left\|200px]]	+	[[Image:3toi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3toi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3toi\| PDB=3toi \| SCENE= }}		{{STRUCTURE_3toi\| PDB=3toi \| SCENE= }}

	===Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization===		===Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22545913}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22545913 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22545913}}		{{ABSTRACT_PUBMED_22545913}}

	==About this Structure==		==About this Structure==
-	[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].	+	[[3toi]] is a 2 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]

	==Reference==		==Reference==

OCA at 09:09, 16 May 2012

OCA — Wed, 16 May 2012 09:09:46 GMT

←Older revision		Revision as of 09:09, 16 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3toi.jpg\|left\|200px]]

-	The ~~entry 3toi~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3toi", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3toi\| PDB=3toi \| SCENE= }}

-	~~Authors: Tam, H.K., Einsle, O.~~	+	===Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization===

-	~~Description~~: ~~Tailoring Enzyme Stability and Exploiting Stability~~-~~Trait Linkage by Iterative Truncation and Optimization~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22545913}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22545913 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22545913}}
		+
		+	==About this Structure==
		+	[[3toi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TOI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022545913</ref><references group="xtra"/>
		+	[[Category: Beta-lactamase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Einsle, O.]]
		+	[[Category: Tam, H K.]]
		+	[[Category: Hydrolase]]

OCA at 05:16, 28 September 2011

OCA — Wed, 28 Sep 2011 05:16:01 GMT

←Older revision		Revision as of 05:16, 28 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3toi is ON HOLD	+	The entry 3toi is ON HOLD until Paper Publication

	Authors: Tam, H.K., Einsle, O.		Authors: Tam, H.K., Einsle, O.

	Description: Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization		Description: Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization

OCA: Protected "3toi" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2011 06:06:20 GMT

Protected "3toi" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:06, 14 September 2011

OCA: New page: '''Unreleased structure''' The entry 3toi is ON HOLD Authors: Tam, H.K., Einsle, O. Description: Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncati...

OCA — Wed, 14 Sep 2011 06:06:19 GMT

New page: '''Unreleased structure''' The entry 3toi is ON HOLD Authors: Tam, H.K., Einsle, O. Description: Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncati...

New page

'''Unreleased structure'''

The entry 3toi is ON HOLD

Authors: Tam, H.K., Einsle, O.

Description: Tailoring Enzyme Stability and Exploiting Stability-Trait Linkage by Iterative Truncation and Optimization