3tpo - Revision history

OCA at 17:33, 1 November 2023

OCA — Wed, 01 Nov 2023 17:33:42 GMT

←Older revision		Revision as of 17:33, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3tpo' size='340' side='right'caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3tpo' size='340' side='right'caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPO FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3tpm\|3tpm]], [[3tpq\|3tpq]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Kpna2 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [https://pdbe.org/3tpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [https://www.ebi.ac.uk/pdbsum/3tpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [https://pdbe.org/3tpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [https://www.ebi.ac.uk/pdbsum/3tpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~IMA2_MOUSE IMA2_MOUSE]~~] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.	+	[https://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Hirano, H]]	+	[[Category: Hirano H]]
-	[[Category: Matsuura, Y]]	+	[[Category: Matsuura Y]]
-	~~[[Category: Nuclear import]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 17:07, 6 July 2022

OCA — Wed, 06 Jul 2022 17:07:59 GMT

←Older revision		Revision as of 17:07, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of D192A/E396A mutant of mouse importin alpha2==		==Crystal structure of D192A/E396A mutant of mouse importin alpha2==
-	<StructureSection load='3tpo' size='340' side='right' caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3tpo' size='340' side='right'caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TPO FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tpm\|3tpm]], [[3tpq\|3tpq]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tpm\|3tpm]], [[3tpq\|3tpq]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [~~http~~://pdbe.org/3tpo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tpo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [https://pdbe.org/3tpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [https://www.ebi.ac.uk/pdbsum/3tpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IMA2_MOUSE IMA2_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.	+	[[https://www.uniprot.org/uniprot/IMA2_MOUSE IMA2_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Importin\|Importin]]	+	*[[Importin 3D structures\|Importin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Hirano, H]]		[[Category: Hirano, H]]

OCA at 01:52, 5 August 2016

OCA — Fri, 05 Aug 2016 01:52:39 GMT

←Older revision		Revision as of 01:52, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of D192A/E396A mutant of mouse importin alpha2==		==Crystal structure of D192A/E396A mutant of mouse importin alpha2==
	<StructureSection load='3tpo' size='340' side='right' caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3tpo' size='340' side='right' caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tpm\|3tpm]], [[3tpq\|3tpq]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tpm\|3tpm]], [[3tpq\|3tpq]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [http://www.ebi.ac.uk/pdbsum/3tpo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [http://pdbe.org/3tpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [http://www.ebi.ac.uk/pdbsum/3tpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tpo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3tpo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Hirano, H]]		[[Category: Hirano, H]]
	[[Category: Matsuura, Y]]		[[Category: Matsuura, Y]]
	[[Category: Nuclear import]]		[[Category: Nuclear import]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 14:51, 25 December 2014

OCA — Thu, 25 Dec 2014 14:51:20 GMT

←Older revision		Revision as of 14:51, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [http://www.ebi.ac.uk/pdbsum/3tpo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [http://www.ebi.ac.uk/pdbsum/3tpo PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IMA2_MOUSE IMA2_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:05, 21 December 2014

OCA — Sun, 21 Dec 2014 07:05:34 GMT

←Older revision		Revision as of 07:05, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3tpo\| PDB=3tpo \| SCENE= }}~~	+	==Crystal structure of D192A/E396A mutant of mouse importin alpha2==
-	===Crystal structure of D192A/E396A mutant of mouse importin alpha2===	+	<StructureSection load='3tpo' size='340' side='right' caption='[[3tpo]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21964294}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TPO FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3tpm\|3tpm]], [[3tpq\|3tpq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Kpna2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tpo RCSB], [http://www.ebi.ac.uk/pdbsum/3tpo PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The coordination of cytoskeletal actin dynamics with gene expression reprogramming is emerging as a crucial mechanism to control diverse cellular processes, including cell migration, differentiation and neuronal circuit assembly. The actin-binding transcriptional coactivator MAL (also known as MRTF-A/MKL1/BSAC) senses G-actin concentration and transduces Rho GTPase signals to serum response factor (SRF). MAL rapidly shuttles between the cytoplasm and the nucleus in unstimulated cells but Rho-induced depletion of G-actin leads to MAL nuclear accumulation and activation of transcription of SRF:MAL-target genes. Although the molecular and structural basis of actin-regulated nucleocytoplasmic shuttling of MAL is not understood fully, it is proposed that nuclear import of MAL is mediated by importin alpha/beta heterodimer, and that G-actin competes with importin alpha/beta for the binding to MAL. Here we present structural, biochemical and cell biological evidence that MAL has a classical bipartite nuclear localization signal (NLS) in the N-terminal 'RPEL' domain containing Arg-Pro-X-X-X-Glu-Leu (RPEL) motifs. The NLS residues of MAL adopt an extended conformation and bind along the surface groove of importin-alpha, interacting with the major- and minor-NLS binding sites. We also present a crystal structure of wild-type MAL RPEL domain in complex with five G-actins. Comparison of the importin-alpha- and actin-complexes revealed that the binding of G-actins to MAL is associated with folding of NLS residues into a helical conformation that is inappropriate for importin-alpha recognition.

-	~~==Function==~~	+	Sensing actin dynamics: structural basis for G-actin-sensitive nuclear import of MAL.,Hirano H, Matsuura Y Biochem Biophys Res Commun. 2011 Oct 22;414(2):373-8. doi:, 10.1016/j.bbrc.2011.09.079. Epub 2011 Sep 21. PMID:21964294<ref>PMID:21964294</ref>
-	~~[[http~~:~~//www.uniprot.org/uniprot/IMA2_MOUSE IMA2_MOUSE]] Functions in~~ nuclear ~~protein~~ import ~~as an adapter protein for nuclear receptor KPNB1~~. ~~Binds specifically and directly to substrates containing either a simple or bipartite NLS motif~~. ~~Docking of the importin/substrate complex to the nuclear pore complex~~ (~~NPC~~) ~~is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran~~-~~dependent mechanism~~. ~~At the nucleoplasmic side of the NPC~~, ~~Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin~~. ~~The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3tpo]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:021964294</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Importin\|Importin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Hirano, H.]]	+	[[Category: Hirano, H]]
-	[[Category: Matsuura, Y.]]	+	[[Category: Matsuura, Y]]
	[[Category: Nuclear import]]		[[Category: Nuclear import]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 08:17, 30 June 2013

OCA — Sun, 30 Jun 2013 08:17:36 GMT

←Older revision		Revision as of 08:17, 30 June 2013
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-	[[Image:3tpo.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3tpo", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_3tpo\| PDB=3tpo \| SCENE= }}		{{STRUCTURE_3tpo\| PDB=3tpo \| SCENE= }}
-
	===Crystal structure of D192A/E396A mutant of mouse importin alpha2===		===Crystal structure of D192A/E396A mutant of mouse importin alpha2===
		+	{{ABSTRACT_PUBMED_21964294}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/IMA2_MOUSE IMA2_MOUSE]] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

	==About this Structure==		==About this Structure==
	[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA].		[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021964294</ref><references group="xtra"/><references/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Hirano, H.]]		[[Category: Hirano, H.]]

OCA at 09:50, 30 October 2011

OCA — Sun, 30 Oct 2011 09:50:59 GMT

←Older revision		Revision as of 09:50, 30 October 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3tpo.png\|left\|200px]]

-	The ~~entry 3tpo~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tpo", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tpo\| PDB=3tpo \| SCENE= }}

-	~~Authors: Hirano, H., Matsuura, Y.~~	+	===Crystal structure of D192A/E396A mutant of mouse importin alpha2===

-	~~Description: Crystal~~ structure ~~of D192A~~/~~E396A mutant of mouse importin alpha2~~	+
		+	==About this Structure==
		+	[[3tpo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TPO OCA].
		+	[[Category: Mus musculus]]
		+	[[Category: Hirano, H.]]
		+	[[Category: Matsuura, Y.]]
		+	[[Category: Nuclear import]]
		+	[[Category: Protein transport]]

OCA: Protected "3tpo" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2011 06:07:37 GMT

Protected "3tpo" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 14 September 2011

OCA: New page: '''Unreleased structure''' The entry 3tpo is ON HOLD Authors: Hirano, H., Matsuura, Y. Description: Crystal structure of D192A/E396A mutant of mouse importin alpha2

OCA — Wed, 14 Sep 2011 06:07:35 GMT

New page: '''Unreleased structure''' The entry 3tpo is ON HOLD Authors: Hirano, H., Matsuura, Y. Description: Crystal structure of D192A/E396A mutant of mouse importin alpha2

New page

'''Unreleased structure'''

The entry 3tpo is ON HOLD

Authors: Hirano, H., Matsuura, Y.

Description: Crystal structure of D192A/E396A mutant of mouse importin alpha2