3ttt - Revision history

OCA at 13:39, 14 March 2024

OCA — Thu, 14 Mar 2024 13:39:08 GMT

←Older revision		Revision as of 13:39, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3ttt' size='340' side='right'caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='3ttt' size='340' side='right'caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TTT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TTT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.58Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3ttu\|3ttu]]~~, [[~~3ttv~~\|~~3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>b1732, JW1721, katE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Catalase Catalase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [https://pdbe.org/3ttt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [https://www.ebi.ac.uk/pdbsum/3ttt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ttt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [https://pdbe.org/3ttt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [https://www.ebi.ac.uk/pdbsum/3ttt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ttt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.	+	[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The monofunctional catalase KatE of Esherichia coli exhibits exceptional resistance to heat denaturation and proteolytic degradation. During an investigation of subtle conformation changes in Arg111 and Phe413 on the proximal side of the heme induced by H(2)O(2), variants at position R111, T115 and F413 were constructed. Because the residues are not situated in the distal side heme cavity where catalysis occurs, significant changes in reactivity were not expected and indeed, only small changes in the kinetic characteristics were observed in all of the variants. However, the F413Y variant was found to have undergone main chain cleavage whereas the R111A, T115A, F413E and F413K variants had not. Two sites of cleavage were identified in the crystal structure and by mass spectrometry at residues 111 and 115. In addition to main chain cleavage, modifications to the side chains of Tyr413, Thr115 and Arg111 were suggested by differences in the electron density maps compared to maps of the native and inactive variant H128N/F413Y. The inactive variant H128N/F413Y and the active variant T115A/F413Y both did not exhibit main chain cleavage and the R11A/F413Y variant exhibited less cleavage. In addition, the apparent modification of three side chains was largely absent in these variants. It is also significant that all three F413 single variants contained heme b suggesting that the fidelity of the phenyl group was important for mediating heme b oxidation to heme d. The reactions are attributed to the introduction of a new reactive center possibly involving a transient radical on Tyr413 formed during catalytic turn over.	+
-		+
-	Mutation of Phe413 to Tyr in catalase KatE from Escherichia coli leads to side chain damage and main chain cleavage.,Jha V, Donald LJ, Loewen PC Arch Biochem Biophys. 2012 Sep 15;525(2):207-14. doi: 10.1016/j.abb.2011.11.022. , Epub 2011 Dec 8. PMID:22172685<ref>PMID:22172685</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ttt" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Catalase 3D structures\|Catalase 3D structures]]		*[[Catalase 3D structures\|Catalase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Catalase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jha, V]]	+	[[Category: Jha V]]
-	[[Category: Loewen~~, P C]]~~	+	[[Category: Loewen PC]]
-	~~[[Category: Heme orientation]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 05:45, 13 July 2022

OCA — Wed, 13 Jul 2022 05:45:04 GMT

←Older revision		Revision as of 05:45, 13 July 2022
Line 1:		Line 1:

	==Structure of F413Y variant of E. coli KatE==		==Structure of F413Y variant of E. coli KatE==
-	<StructureSection load='3ttt' size='340' side='right' caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>	+	<StructureSection load='3ttt' size='340' side='right'caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TTT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TTT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ttu\|3ttu]], [[3ttv\|3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ttu\|3ttu]], [[3ttv\|3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Catalase Catalase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Catalase Catalase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [~~http~~://pdbe.org/3ttt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ttt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ttt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [https://pdbe.org/3ttt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [https://www.ebi.ac.uk/pdbsum/3ttt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ttt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.	+	[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Catalase\|Catalase]]	+	*[[Catalase 3D structures\|Catalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Catalase]]		[[Category: Catalase]]
		+	[[Category: Large Structures]]
	[[Category: Jha, V]]		[[Category: Jha, V]]
	[[Category: Loewen, P C]]		[[Category: Loewen, P C]]
	[[Category: Heme orientation]]		[[Category: Heme orientation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 03:12, 5 August 2016

OCA — Fri, 05 Aug 2016 03:12:38 GMT

←Older revision		Revision as of 03:12, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of F413Y variant of E. coli KatE==		==Structure of F413Y variant of E. coli KatE==
	<StructureSection load='3ttt' size='340' side='right' caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='3ttt' size='340' side='right' caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TTT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TTT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ttu\|3ttu]], [[3ttv\|3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ttu\|3ttu]], [[3ttv\|3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [http://www.ebi.ac.uk/pdbsum/3ttt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [http://pdbe.org/3ttt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [http://www.ebi.ac.uk/pdbsum/3ttt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ttt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ttt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Catalase]]		[[Category: Catalase]]
-	[[Category: Escherichia coli]]
	[[Category: Jha, V]]		[[Category: Jha, V]]
	[[Category: Loewen, P C]]		[[Category: Loewen, P C]]
	[[Category: Heme orientation]]		[[Category: Heme orientation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 22:23, 24 December 2014

OCA — Wed, 24 Dec 2014 22:23:17 GMT

←Older revision		Revision as of 22:23, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [http://www.ebi.ac.uk/pdbsum/3ttt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [http://www.ebi.ac.uk/pdbsum/3ttt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:31, 9 December 2014

OCA — Tue, 09 Dec 2014 15:31:14 GMT

←Older revision		Revision as of 15:31, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3ttt.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of F413Y variant of E. coli KatE==
		+	<StructureSection load='3ttt' size='340' side='right' caption='[[3ttt]], [[Resolution\|resolution]] 1.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TTT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ttu\|3ttu]], [[3ttv\|3ttv]], [[3ttw\|3ttw]], [[3ttx\|3ttx]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ttt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ttt RCSB], [http://www.ebi.ac.uk/pdbsum/3ttt PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The monofunctional catalase KatE of Esherichia coli exhibits exceptional resistance to heat denaturation and proteolytic degradation. During an investigation of subtle conformation changes in Arg111 and Phe413 on the proximal side of the heme induced by H(2)O(2), variants at position R111, T115 and F413 were constructed. Because the residues are not situated in the distal side heme cavity where catalysis occurs, significant changes in reactivity were not expected and indeed, only small changes in the kinetic characteristics were observed in all of the variants. However, the F413Y variant was found to have undergone main chain cleavage whereas the R111A, T115A, F413E and F413K variants had not. Two sites of cleavage were identified in the crystal structure and by mass spectrometry at residues 111 and 115. In addition to main chain cleavage, modifications to the side chains of Tyr413, Thr115 and Arg111 were suggested by differences in the electron density maps compared to maps of the native and inactive variant H128N/F413Y. The inactive variant H128N/F413Y and the active variant T115A/F413Y both did not exhibit main chain cleavage and the R11A/F413Y variant exhibited less cleavage. In addition, the apparent modification of three side chains was largely absent in these variants. It is also significant that all three F413 single variants contained heme b suggesting that the fidelity of the phenyl group was important for mediating heme b oxidation to heme d. The reactions are attributed to the introduction of a new reactive center possibly involving a transient radical on Tyr413 formed during catalytic turn over.

-	~~{{STRUCTURE_3ttt\| PDB=3ttt \| SCENE= }}~~	+	Mutation of Phe413 to Tyr in catalase KatE from Escherichia coli leads to side chain damage and main chain cleavage.,Jha V, Donald LJ, Loewen PC Arch Biochem Biophys. 2012 Sep 15;525(2):207-14. doi: 10.1016/j.abb.2011.11.022. , Epub 2011 Dec 8. PMID:22172685<ref>PMID:22172685</ref>

-	~~===Structure~~ of ~~F413Y variant~~ of E. ~~coli KatE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22172685}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ttt]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].~~	+

	==See Also==		==See Also==
	*[[Catalase\|Catalase]]		*[[Catalase\|Catalase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Jha, V.]]	+	[[Category: Jha, V]]
-	[[Category: Loewen, P C.]]	+	[[Category: Loewen, P C]]
	[[Category: Heme orientation]]		[[Category: Heme orientation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 12:53, 27 February 2013

OCA — Wed, 27 Feb 2013 12:53:58 GMT

←Older revision		Revision as of 12:53, 27 February 2013
Line 5:		Line 5:
	===Structure of F413Y variant of E. coli KatE===		===Structure of F413Y variant of E. coli KatE===

		+	{{ABSTRACT_PUBMED_22172685}}

	==About this Structure==		==About this Structure==

OCA at 09:44, 20 October 2012

OCA — Sat, 20 Oct 2012 09:44:09 GMT

←Older revision		Revision as of 09:44, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3ttt]] is a 4 chain structure ~~of [[Catalase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].	+	[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].

	==See Also==		==See Also==

OCA at 23:47, 25 July 2012

OCA — Wed, 25 Jul 2012 23:47:59 GMT

←Older revision		Revision as of 23:47, 25 July 2012
Line 1:		Line 1:
	[[Image:3ttt.png\|left\|200px]]		[[Image:3ttt.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ttt", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ttt\| PDB=3ttt \| SCENE= }}		{{STRUCTURE_3ttt\| PDB=3ttt \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].	+	[[3ttt]] is a 4 chain structure of [[Catalase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].
		+
		+	==See Also==
		+	*[[Catalase\|Catalase]]
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 09:53, 30 October 2011

OCA — Sun, 30 Oct 2011 09:53:10 GMT

←Older revision		Revision as of 09:53, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ttt.png\|left\|200px]]

-	The ~~entry 3ttt~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ttt", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ttt\| PDB=3ttt \| SCENE= }}

-	~~Authors: Loewen, P.C., Jha, V~~.	+	===Structure of F413Y variant of E. coli KatE===

-	~~Description:~~ Structure ~~of F413 variants of E~~. coli ~~KatE~~	+
		+	==About this Structure==
		+	[[3ttt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TTT OCA].
		+	[[Category: Catalase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Jha, V.]]
		+	[[Category: Loewen, P C.]]
		+	[[Category: Heme orientation]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "3ttt" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2011 05:35:18 GMT

Protected "3ttt" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:35, 21 September 2011