3tzk - Revision history

OCA at 10:16, 1 March 2024

OCA — Fri, 01 Mar 2024 10:16:13 GMT

←Older revision		Revision as of 10:16, 1 March 2024
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [https://pdbe.org/3tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [https://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [https://pdbe.org/3tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [https://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	beta-ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. The activation of the enzyme is usually linked to the formation of a catalytic triad and cofactor binding, and crystal structures of FabG from different organisms have been captured in either active or inactive conformations. However, the structural elements which enable activation of FabG require further exploration. Here we report structural, enzymatic, and binding studies of vcFabG, the FabG protein found in the causative agent of cholera, Vibrio cholerae. vcFabG exists predominantly as a dimer in solution and is able to self-associate to form tetramers, which is the state seen in the crystal structure. The formation of the tetramer may be promoted by the presence of the cofactor NADP(H). The transition between dimeric and tetrameric states of vcFabG is related to changes in the conformations of the alpha4/alpha5 helices on the dimer-dimer interface. Two glycine residues adjacent to the dimer interface (G92 and G141) are identified as the hinge for the conformational changes, while the catalytic tyrosine (Y155) and a glutamine residue that forms hydrogen bonds to both loops beta4-alpha4 and beta5-alpha5 (Q152) stabilize the active conformation. The functions of the aforementioned residues were confirmed by the binding and enzymatic assays for the corresponding mutants. IMPORTANCE: This paper describes structural, enzymatic, and binding studies of FabG from Vibrio cholera. In this work we dissect the structural elements responsible for the activation of vcFabG. The structural information provided here is essential for the development of antibiotics specifically targeting bacteria FabG, especially for the multi-drug resistant strains of V. cholerae.
-
-	Dissecting the structural elements for the activation of beta-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae.,Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W J Bacteriol. 2015 Nov 9. pii: JB.00360-15. PMID:26553852<ref>PMID:26553852</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3tzk" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:47, 17 January 2024

OCA — Wed, 17 Jan 2024 12:47:57 GMT

←Older revision		Revision as of 12:47, 17 January 2024
Line 3:		Line 3:
	<StructureSection load='3tzk' size='340' side='right'caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3tzk' size='340' side='right'caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Vibcj Vibcj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_MJ-1236 Vibrio cholerae MJ-1236]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TZK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>VC2021, VCD_002346 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~593588 VIBCJ])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [https://pdbe.org/3tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [https://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [https://pdbe.org/3tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [https://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibcj~~]]	+	[[Category: Vibrio cholerae MJ-1236]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Chruszcz M]]
-	[[Category: Chruszcz, M]]	+	[[Category: Domagalski M]]
-	[[Category: Domagalski, M]]	+	[[Category: Grabowski M]]
-	[[Category: Grabowski, M]]	+	[[Category: Hou J]]
-	[[Category: Hou, J]]	+	[[Category: Minor W]]
-	[[Category: Minor, W]]	+	[[Category: Zheng H]]
-	[[Category: Zheng, H]]	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Fabg]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Vibrio cholerae~~]]	+

OCA at 10:00, 13 April 2022

OCA — Wed, 13 Apr 2022 10:00:25 GMT

←Older revision		Revision as of 10:00, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae==		==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae==
-	<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3tzk' size='340' side='right'caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Vibcj Vibcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3TZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibcj Vibcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TZK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [~~http~~://pdbe.org/3tzk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [https://pdbe.org/3tzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [https://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Vibcj]]		[[Category: Vibcj]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 09:58, 8 November 2017

OCA — Wed, 08 Nov 2017 09:58:08 GMT

←Older revision		Revision as of 09:58, 8 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae==		==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae==
	<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://pdbe.org/3tzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://pdbe.org/3tzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tzk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:49, 30 November 2015

OCA — Mon, 30 Nov 2015 20:49:08 GMT

←Older revision		Revision as of 20:49, 30 November 2015
Line 2:		Line 2:
	<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae Vibrio cholerae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibcj Vibcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~666 Vibrio cholerae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://pdbe.org/3tzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. The activation of the enzyme is usually linked to the formation of a catalytic triad and cofactor binding, and crystal structures of FabG from different organisms have been captured in either active or inactive conformations. However, the structural elements which enable activation of FabG require further exploration. Here we report structural, enzymatic, and binding studies of vcFabG, the FabG protein found in the causative agent of cholera, Vibrio cholerae. vcFabG exists predominantly as a dimer in solution and is able to self-associate to form tetramers, which is the state seen in the crystal structure. The formation of the tetramer may be promoted by the presence of the cofactor NADP(H). The transition between dimeric and tetrameric states of vcFabG is related to changes in the conformations of the alpha4/alpha5 helices on the dimer-dimer interface. Two glycine residues adjacent to the dimer interface (G92 and G141) are identified as the hinge for the conformational changes, while the catalytic tyrosine (Y155) and a glutamine residue that forms hydrogen bonds to both loops beta4-alpha4 and beta5-alpha5 (Q152) stabilize the active conformation. The functions of the aforementioned residues were confirmed by the binding and enzymatic assays for the corresponding mutants. IMPORTANCE: This paper describes structural, enzymatic, and binding studies of FabG from Vibrio cholera. In this work we dissect the structural elements responsible for the activation of vcFabG. The structural information provided here is essential for the development of antibiotics specifically targeting bacteria FabG, especially for the multi-drug resistant strains of V. cholerae.
		+
		+	Dissecting the structural elements for the activation of beta-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae.,Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W J Bacteriol. 2015 Nov 9. pii: JB.00360-15. PMID:26553852<ref>PMID:26553852</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3tzk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae~~]]	+	[[Category: Vibcj]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
Line 22:		Line 33:
	[[Category: Fabg]]		[[Category: Fabg]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
		+	[[Category: Vibrio cholerae]]

OCA at 09:50, 4 January 2015

OCA — Sun, 04 Jan 2015 09:50:47 GMT

←Older revision		Revision as of 09:50, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>
-	<table>	+	</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Chruszcz, M.]]	+	[[Category: Chruszcz, M]]
-	[[Category: Domagalski, M.]]	+	[[Category: Domagalski, M]]
-	[[Category: Grabowski, M.]]	+	[[Category: Grabowski, M]]
-	[[Category: Hou, J.]]	+	[[Category: Hou, J]]
-	[[Category: Minor, W.]]	+	[[Category: Minor, W]]
-	[[Category: Zheng, H~~.]]~~	+	[[Category: Zheng, H]]
-	~~[[Category: Center for structural genomics of infectious disease~~]]	+
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Fabg]]		[[Category: Fabg]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Structural genomic]]
-	[[Category: Vibrio cholerae]]

OCA at 06:26, 5 June 2014

OCA — Thu, 05 Jun 2014 06:26:19 GMT

←Older revision		Revision as of 06:26, 5 June 2014
Line 1:		Line 1:
-	~~[[Image:3tzk.png\|left\|200px]]~~	+	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae==
-		+	<StructureSection load='3tzk' size='340' side='right' caption='[[3tzk]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~<!--~~	+	== Structural highlights ==
-	~~The line below this paragraph, containing "STRUCTURE_3tzk", creates the "Structure Box" on the page.~~	+	<table><tr><td colspan='2'>[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TZK FirstGlance]. <br>
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene><br>
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC2021, VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
-	~~or leave the SCENE parameter empty for the default display.~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	~~-->~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tzk RCSB], [http://www.ebi.ac.uk/pdbsum/3tzk PDBsum]</span></td></tr>
-	~~{{STRUCTURE_3tzk\| PDB=3tzk \| SCENE= }}~~	+	<table>
-		+	__TOC__
-	===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae===	+	</StructureSection>
-		+
-		+
-	==~~About this Structure~~==	+
-	[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA].	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 10:26, 30 October 2011

OCA — Sun, 30 Oct 2011 10:26:07 GMT

←Older revision		Revision as of 10:26, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3tzk.png\|left\|200px]]

-	The ~~entry 3tzk~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3tzk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3tzk\| PDB=3tzk \| SCENE= }}

-	~~Authors: Hou, J., Chruszcz, M., Zheng, H., Grabowski, M., Domagalski, M., Anderson, W.F., Minor, W., Center for Structural Genomics~~ of ~~Infectious Diseases~~ (~~CSGID~~)	+	===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae===

-	~~Description: Crystal~~ structure ~~of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A)~~ from Vibrio cholerae	+
		+	==About this Structure==
		+	[[3tzk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TZK OCA].
		+	[[Category: Vibrio cholerae]]
		+	[[Category: Anderson, W F.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Chruszcz, M.]]
		+	[[Category: Domagalski, M.]]
		+	[[Category: Grabowski, M.]]
		+	[[Category: Hou, J.]]
		+	[[Category: Minor, W.]]
		+	[[Category: Zheng, H.]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Csgid]]
		+	[[Category: Fabg]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Structural genomic]]
		+	[[Category: Vibrio cholerae]]

OCA: Protected "3tzk" [edit=sysop:move=sysop]

OCA — Wed, 05 Oct 2011 06:14:33 GMT

Protected "3tzk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:14, 5 October 2011

OCA: New page: '''Unreleased structure''' The entry 3tzk is ON HOLD Authors: Hou, J., Chruszcz, M., Zheng, H., Grabowski, M., Domagalski, M., Anderson, W.F., Minor, W., Center for Structural Genomics ...

OCA — Wed, 05 Oct 2011 06:14:32 GMT

New page: '''Unreleased structure''' The entry 3tzk is ON HOLD Authors: Hou, J., Chruszcz, M., Zheng, H., Grabowski, M., Domagalski, M., Anderson, W.F., Minor, W., Center for Structural Genomics ...

New page

'''Unreleased structure'''

The entry 3tzk is ON HOLD

Authors: Hou, J., Chruszcz, M., Zheng, H., Grabowski, M., Domagalski, M., Anderson, W.F., Minor, W., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae