3u0l - Revision history

OCA at 13:44, 14 March 2024

OCA — Thu, 14 Mar 2024 13:44:57 GMT

←Older revision		Revision as of 13:44, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref>		[https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution crystal structures of mRuby, the red sensor RCaMP, and the recently published red GECI R-GECO1 give insight into the chromophore environments of the Ca(2+)-bound state of the sensors and the engineered protein domain interfaces of the different indicators. We characterized the biophysical properties and performance of RCaMP sensors in vitro and in vivo in Caenorhabditis elegans, Drosophila larvae, and larval zebrafish. Further, we demonstrate 2-color calcium imaging both within the same cell (registering mitochondrial and somatic [Ca(2+)]) and between two populations of cells: neurons and astrocytes. Finally, we perform integrated optogenetics experiments, wherein neural activation via channelrhodopsin-2 (ChR2) or a red-shifted variant, and activity imaging via RCaMP or GCaMP, are conducted simultaneously, with the ChR2/RCaMP pair providing independently addressable spectral channels. Using this paradigm, we measure calcium responses of naturalistic and ChR2-evoked muscle contractions in vivo in crawling C. elegans. We systematically compare the RCaMP sensors to R-GECO1, in terms of action potential-evoked fluorescence increases in neurons, photobleaching, and photoswitching. R-GECO1 displays higher Ca(2+) affinity and larger dynamic range than RCaMP, but exhibits significant photoactivation with blue and green light, suggesting that integrated channelrhodopsin-based optogenetics using R-GECO1 may be subject to artifact. Finally, we create and test blue, cyan, and yellow variants engineered from GCaMP by rational design. This engineered set of chromatic variants facilitates new experiments in functional imaging and optogenetics.
-
-	Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.,Akerboom J, Carreras Calderon N, Tian L, Wabnig S, Prigge M, Tolo J, Gordus A, Orger MB, Severi KE, Macklin JJ, Patel R, Pulver SR, Wardill TJ, Fischer E, Schuler C, Chen TW, Sarkisyan KS, Marvin JS, Bargmann CI, Kim DS, Kugler S, Lagnado L, Hegemann P, Gottschalk A, Schreiter ER, Looger LL Front Mol Neurosci. 2013;6:2. doi: 10.3389/fnmol.2013.00002. Epub 2013 Mar 4. PMID:23459413<ref>PMID:23459413</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3u0l" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:17, 6 December 2023

OCA — Wed, 06 Dec 2023 08:17:18 GMT

←Older revision		Revision as of 08:17, 6 December 2023
Line 3:		Line 3:
	<StructureSection load='3u0l' size='340' side='right'caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='3u0l' size='340' side='right'caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bubble-tip_anemone Bubble-tip anemone~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene~~></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3u0m\|3u0m]], [[3u0n\|3u0n]]</div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [https://pdbe.org/3u0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [https://www.ebi.ac.uk/pdbsum/3u0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [https://pdbe.org/3u0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [https://www.ebi.ac.uk/pdbsum/3u0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bubble-tip anemone~~]]	+	[[Category: Entacmaea quadricolor]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akerboom, J]]	+	[[Category: Akerboom J]]
-	[[Category: Looger~~, L L~~]]	+	[[Category: Looger LL]]
-	[[Category: Schreiter~~, E R]]~~	+	[[Category: Schreiter ER]]
-	~~[[Category: Fluorescent protein~~]]	+

OCA at 05:52, 13 July 2022

OCA — Wed, 13 Jul 2022 05:52:47 GMT

←Older revision		Revision as of 05:52, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5==		==Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5==
-	<StructureSection load='3u0l' size='340' side='right' caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>	+	<StructureSection load='3u0l' size='340' side='right'caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u0l]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3U0L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u0m\|3u0m]], [[3u0n\|3u0n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3u0m\|3u0m]], [[3u0n\|3u0n]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [~~http~~://pdbe.org/3u0l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3u0l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [https://pdbe.org/3u0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [https://www.ebi.ac.uk/pdbsum/3u0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0l ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bubble-tip anemone]]		[[Category: Bubble-tip anemone]]
		+	[[Category: Large Structures]]
	[[Category: Akerboom, J]]		[[Category: Akerboom, J]]
	[[Category: Looger, L L]]		[[Category: Looger, L L]]
	[[Category: Schreiter, E R]]		[[Category: Schreiter, E R]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 18:52, 11 August 2016

OCA — Thu, 11 Aug 2016 18:52:13 GMT

←Older revision		Revision as of 18:52, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5==		==Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5==
	<StructureSection load='3u0l' size='340' side='right' caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='3u0l' size='340' side='right' caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u0m\|3u0m]], [[3u0n\|3u0n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u0m\|3u0m]], [[3u0n\|3u0n]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [http://www.ebi.ac.uk/pdbsum/3u0l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [http://pdbe.org/3u0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [http://www.ebi.ac.uk/pdbsum/3u0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0l ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3u0l" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:28, 4 January 2015

OCA — Sun, 04 Jan 2015 10:28:50 GMT

←Older revision		Revision as of 10:28, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3u0l\| PDB=3u0l \| SCENE= }}~~	+	==Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5==
-	===Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5===	+	<StructureSection load='3u0l' size='340' side='right' caption='[[3u0l]], [[Resolution\|resolution]] 1.25Å' scene=''>
-	{~~{ABSTRACT_PUBMED_23459413}~~}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3u0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U0L FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3u0m\|3u0m]], [[3u0n\|3u0n]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u0l RCSB], [http://www.ebi.ac.uk/pdbsum/3u0l PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution crystal structures of mRuby, the red sensor RCaMP, and the recently published red GECI R-GECO1 give insight into the chromophore environments of the Ca(2+)-bound state of the sensors and the engineered protein domain interfaces of the different indicators. We characterized the biophysical properties and performance of RCaMP sensors in vitro and in vivo in Caenorhabditis elegans, Drosophila larvae, and larval zebrafish. Further, we demonstrate 2-color calcium imaging both within the same cell (registering mitochondrial and somatic [Ca(2+)]) and between two populations of cells: neurons and astrocytes. Finally, we perform integrated optogenetics experiments, wherein neural activation via channelrhodopsin-2 (ChR2) or a red-shifted variant, and activity imaging via RCaMP or GCaMP, are conducted simultaneously, with the ChR2/RCaMP pair providing independently addressable spectral channels. Using this paradigm, we measure calcium responses of naturalistic and ChR2-evoked muscle contractions in vivo in crawling C. elegans. We systematically compare the RCaMP sensors to R-GECO1, in terms of action potential-evoked fluorescence increases in neurons, photobleaching, and photoswitching. R-GECO1 displays higher Ca(2+) affinity and larger dynamic range than RCaMP, but exhibits significant photoactivation with blue and green light, suggesting that integrated channelrhodopsin-based optogenetics using R-GECO1 may be subject to artifact. Finally, we create and test blue, cyan, and yellow variants engineered from GCaMP by rational design. This engineered set of chromatic variants facilitates new experiments in functional imaging and optogenetics.

-	~~==About this Structure==~~	+	Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.,Akerboom J, Carreras Calderon N, Tian L, Wabnig S, Prigge M, Tolo J, Gordus A, Orger MB, Severi KE, Macklin JJ, Patel R, Pulver SR, Wardill TJ, Fischer E, Schuler C, Chen TW, Sarkisyan KS, Marvin JS, Bargmann CI, Kim DS, Kugler S, Lagnado L, Hegemann P, Gottschalk A, Schreiter ER, Looger LL Front Mol Neurosci. 2013;6:2. doi: 10.3389/fnmol.2013.00002. Epub 2013 Mar 4. PMID:23459413<ref>PMID:23459413</ref>
-	~~[[3u0l]] is a 1 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bubble-tip_anemone Bubble-tip anemone]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3U0L OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023459413</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bubble-tip anemone]]		[[Category: Bubble-tip anemone]]
-	[[Category: Akerboom, J.]]	+	[[Category: Akerboom, J]]
-	[[Category: Looger, L L.]]	+	[[Category: Looger, L L]]
-	[[Category: Schreiter, E R.]]	+	[[Category: Schreiter, E R]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 05:40, 29 January 2014

OCA — Wed, 29 Jan 2014 05:40:02 GMT

←Older revision		Revision as of 05:40, 29 January 2014
Line 1:		Line 1:
-	[[Image:3u0l.jpg\|left\|200px]]
-
	{{STRUCTURE_3u0l\| PDB=3u0l \| SCENE= }}		{{STRUCTURE_3u0l\| PDB=3u0l \| SCENE= }}
-
	===Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5===		===Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5===
		+	{{ABSTRACT_PUBMED_23459413}}

		+	==About this Structure==
		+	[[3u0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA].

-	==~~About this Structure~~==	+	==Reference==
-	~~[[3u0l]] is a 1 chain structure with sequence from [http~~://~~en.wikipedia.org~~/~~wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA].~~	+	<ref group="xtra">PMID:023459413</ref><references group="xtra"/><references/>
-	[[Category: ~~Entacmaea quadricolor~~]]	+	[[Category: Bubble-tip anemone]]
	[[Category: Akerboom, J.]]		[[Category: Akerboom, J.]]
	[[Category: Looger, L L.]]		[[Category: Looger, L L.]]
	[[Category: Schreiter, E R.]]		[[Category: Schreiter, E R.]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 06:20, 3 October 2012

OCA — Wed, 03 Oct 2012 06:20:14 GMT

←Older revision		Revision as of 06:20, 3 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3u0l.jpg\|left\|200px]]

-	~~The entry~~ 3u0l ~~is ON HOLD~~ ~~until Sep 28 2013~~	+	{{STRUCTURE_3u0l\| PDB=3u0l \| SCENE= }}

-	~~Authors: Akerboom~~, J., ~~Looger, L.L., Schreiter, E.R~~.	+	===Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5===

-	~~Description: Crystal~~ structure ~~of the engineered fluorescent protein mRuby~~, ~~crystal form 1~~, ~~pH 4~~.5	+
		+	==About this Structure==
		+	[[3u0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0L OCA].
		+	[[Category: Entacmaea quadricolor]]
		+	[[Category: Akerboom, J.]]
		+	[[Category: Looger, L L.]]
		+	[[Category: Schreiter, E R.]]
		+	[[Category: Fluorescent protein]]

OCA at 04:37, 12 September 2012

OCA — Wed, 12 Sep 2012 04:37:17 GMT

←Older revision		Revision as of 04:37, 12 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3u0l is ON HOLD until ~~Paper Publication~~	+	The entry 3u0l is ON HOLD until Sep 28 2013

	Authors: Akerboom, J., Looger, L.L., Schreiter, E.R.		Authors: Akerboom, J., Looger, L.L., Schreiter, E.R.

	Description: Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5		Description: Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5

OCA at 08:47, 30 October 2011

OCA — Sun, 30 Oct 2011 08:47:06 GMT

←Older revision		Revision as of 08:47, 30 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3u0l is ON HOLD	+	The entry 3u0l is ON HOLD until Paper Publication

	Authors: Akerboom, J., Looger, L.L., Schreiter, E.R.		Authors: Akerboom, J., Looger, L.L., Schreiter, E.R.

	Description: Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5		Description: Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5

OCA: Protected "3u0l" [edit=sysop:move=sysop]

OCA — Wed, 05 Oct 2011 06:15:34 GMT

Protected "3u0l" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 5 October 2011