3u9r - Revision history

OCA at 10:19, 1 March 2024

OCA — Fri, 01 Mar 2024 10:19:03 GMT

←Older revision		Revision as of 10:19, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3u9r' size='340' side='right'caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3u9r' size='340' side='right'caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U9R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U9R FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>liuB, PA2014 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [https://pdbe.org/3u9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [https://www.ebi.ac.uk/pdbsum/3u9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [https://pdbe.org/3u9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [https://www.ebi.ac.uk/pdbsum/3u9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9r ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9I297_PSEAE Q9I297_PSEAE]
-	3-Methylcrotonyl-CoA carboxylase (MCC), a member of the biotin-dependent carboxylase superfamily, is essential for the metabolism of leucine, and deficient mutations in this enzyme are linked to methylcrotonylglycinuria (MCG) and other serious diseases in humans. MCC has strong sequence conservation with propionyl-CoA carboxylase (PCC), and their holoenzymes are both 750-kilodalton (kDa) alpha(6)beta(6) dodecamers. Therefore the architecture of the MCC holoenzyme is expected to be highly similar to that of PCC. Here we report the crystal structures of the Pseudomonas aeruginosa MCC (PaMCC) holoenzyme, alone and in complex with coenzyme A. Surprisingly, the structures show that the architecture and overall shape of PaMCC are markedly different when compared to PCC. The alpha-subunits show trimeric association in the PaMCC holoenzyme, whereas they have no contacts with each other in PCC. Moreover, the positions of the two domains in the beta-subunit of PaMCC are swapped relative to those in PCC. This structural information establishes a foundation for understanding the disease-causing mutations of MCC and provides new insights into the catalytic mechanism and evolution of biotin-dependent carboxylases. The large structural differences between MCC and PCC also have general implications for the relationship between sequence conservation and structural similarity.	+
-		+
-	~~An unanticipated architecture of the 750-kDa alpha6beta6 holoenzyme of 3-methylcrotonyl-CoA carboxylase.,Huang CS, Ge P, Zhou ZH, Tong L Nature. 2011 Dec 11;481(7380)~~:~~219-23. doi: 10.1038~~/~~nature10691. PMID:22158123<ref>PMID:22158123<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3u9r" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Methylcrotonoyl-CoA carboxylase~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Huang~~, C S~~]]	+	[[Category: Huang CS]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	~~[[Category: Beta-beta-alpha superhelix]]~~	+
-	~~[[Category: Carboxyltransferase]]~~	+
-	~~[[Category: Ligase~~]]	+

OCA at 06:04, 13 July 2022

OCA — Wed, 13 Jul 2022 06:04:12 GMT

←Older revision		Revision as of 06:04, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit==		==Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit==
-	<StructureSection load='3u9r' size='340' side='right' caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='3u9r' size='340' side='right'caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3U9R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U9R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">liuB, PA2014 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">liuB, PA2014 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [~~http~~://pdbe.org/3u9r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3u9r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9r ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [https://pdbe.org/3u9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [https://www.ebi.ac.uk/pdbsum/3u9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9r ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Methylcrotonoyl-CoA carboxylase]]		[[Category: Methylcrotonoyl-CoA carboxylase]]
	[[Category: Huang, C S]]		[[Category: Huang, C S]]

OCA at 19:04, 10 December 2016

OCA — Sat, 10 Dec 2016 19:04:27 GMT

←Older revision		Revision as of 19:04, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit==		==Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit==
	<StructureSection load='3u9r' size='340' side='right' caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3u9r' size='340' side='right' caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U9R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U9R FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">liuB, PA2014 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">liuB, PA2014 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [http://www.ebi.ac.uk/pdbsum/3u9r PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [http://pdbe.org/3u9r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [http://www.ebi.ac.uk/pdbsum/3u9r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u9r ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3u9r" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Methylcrotonoyl-CoA carboxylase]]		[[Category: Methylcrotonoyl-CoA carboxylase]]
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Huang, C S]]		[[Category: Huang, C S]]
	[[Category: Tong, L]]		[[Category: Tong, L]]

OCA at 14:55, 9 December 2014

OCA — Tue, 09 Dec 2014 14:55:20 GMT

←Older revision		Revision as of 14:55, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3u9r.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit==
		+	<StructureSection load='3u9r' size='340' side='right' caption='[[3u9r]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3u9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U9R FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n6r\|3n6r]], [[3u9s\|3u9s]], [[3u9t\|3u9t]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">liuB, PA2014 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylcrotonoyl-CoA_carboxylase Methylcrotonoyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.4 6.4.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u9r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u9r RCSB], [http://www.ebi.ac.uk/pdbsum/3u9r PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	3-Methylcrotonyl-CoA carboxylase (MCC), a member of the biotin-dependent carboxylase superfamily, is essential for the metabolism of leucine, and deficient mutations in this enzyme are linked to methylcrotonylglycinuria (MCG) and other serious diseases in humans. MCC has strong sequence conservation with propionyl-CoA carboxylase (PCC), and their holoenzymes are both 750-kilodalton (kDa) alpha(6)beta(6) dodecamers. Therefore the architecture of the MCC holoenzyme is expected to be highly similar to that of PCC. Here we report the crystal structures of the Pseudomonas aeruginosa MCC (PaMCC) holoenzyme, alone and in complex with coenzyme A. Surprisingly, the structures show that the architecture and overall shape of PaMCC are markedly different when compared to PCC. The alpha-subunits show trimeric association in the PaMCC holoenzyme, whereas they have no contacts with each other in PCC. Moreover, the positions of the two domains in the beta-subunit of PaMCC are swapped relative to those in PCC. This structural information establishes a foundation for understanding the disease-causing mutations of MCC and provides new insights into the catalytic mechanism and evolution of biotin-dependent carboxylases. The large structural differences between MCC and PCC also have general implications for the relationship between sequence conservation and structural similarity.

-	~~{{STRUCTURE_3u9r\| PDB=3u9r \| SCENE= }}~~	+	An unanticipated architecture of the 750-kDa alpha6beta6 holoenzyme of 3-methylcrotonyl-CoA carboxylase.,Huang CS, Ge P, Zhou ZH, Tong L Nature. 2011 Dec 11;481(7380):219-23. doi: 10.1038/nature10691. PMID:22158123<ref>PMID:22158123</ref>

-	~~===Crystal structure~~ of P. ~~aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22158123}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3u9r]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA].~~	+	</StructureSection>
	[[Category: Methylcrotonoyl-CoA carboxylase]]		[[Category: Methylcrotonoyl-CoA carboxylase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Huang, C S.]]	+	[[Category: Huang, C S]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
	[[Category: Beta-beta-alpha superhelix]]		[[Category: Beta-beta-alpha superhelix]]
	[[Category: Carboxyltransferase]]		[[Category: Carboxyltransferase]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 11:41, 23 January 2013

OCA — Wed, 23 Jan 2013 11:41:14 GMT

←Older revision		Revision as of 11:41, 23 January 2013
Line 1:		Line 1:
-	[[Image:3u9r.~~jpg~~\|left\|200px]]	+	[[Image:3u9r.png\|left\|200px]]

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-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3u9r\| PDB=3u9r \| SCENE= }}		{{STRUCTURE_3u9r\| PDB=3u9r \| SCENE= }}

	===Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit===		===Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit===

		+	{{ABSTRACT_PUBMED_22158123}}

	==About this Structure==		==About this Structure==

OCA at 11:53, 14 December 2011

OCA — Wed, 14 Dec 2011 11:53:42 GMT

←Older revision		Revision as of 11:53, 14 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3u9r.jpg\|left\|200px]]

-	The ~~entry 3u9r~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3u9r", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3u9r\| PDB=3u9r \| SCENE= }}

-	~~Authors: Huang, C.S~~., ~~Tong, L.~~	+	===Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit===

-	~~Description: Crystal~~ structure ~~of P~~. aeruginosa 3-~~methylcrotonyl~~-CoA carboxylase ~~(MCC)~~, beta ~~subunit~~	+
		+	==About this Structure==
		+	[[3u9r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U9R OCA].
		+	[[Category: Methylcrotonoyl-CoA carboxylase]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Huang, C S.]]
		+	[[Category: Tong, L.]]
		+	[[Category: Beta-beta-alpha superhelix]]
		+	[[Category: Carboxyltransferase]]
		+	[[Category: Ligase]]

OCA at 11:15, 9 November 2011

OCA — Wed, 09 Nov 2011 11:15:16 GMT

←Older revision		Revision as of 11:15, 9 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3u9r is ON HOLD	+	The entry 3u9r is ON HOLD until Paper Publication

	Authors: Huang, C.S., Tong, L.		Authors: Huang, C.S., Tong, L.

-	Description: Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase, beta subunit	+	Description: Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase (MCC), beta subunit

OCA: Protected "3u9r" [edit=sysop:move=sysop]

OCA — Sun, 30 Oct 2011 08:56:18 GMT

Protected "3u9r" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:56, 30 October 2011

OCA: New page: '''Unreleased structure''' The entry 3u9r is ON HOLD Authors: Huang, C.S., Tong, L. Description: Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase, beta subunit

OCA — Sun, 30 Oct 2011 08:56:17 GMT

New page: '''Unreleased structure''' The entry 3u9r is ON HOLD Authors: Huang, C.S., Tong, L. Description: Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase, beta subunit

New page

'''Unreleased structure'''

The entry 3u9r is ON HOLD

Authors: Huang, C.S., Tong, L.

Description: Crystal structure of P. aeruginosa 3-methylcrotonyl-CoA carboxylase, beta subunit