3uaj - Revision history

OCA at 13:52, 14 March 2024

OCA — Thu, 14 Mar 2024 13:52:36 GMT

←Older revision		Revision as of 13:52, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uaj' size='340' side='right'caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>		<StructureSection load='3uaj' size='340' side='right'caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chimpansee_troglodytes Chimpansee troglodytes~~] and [https://en.wikipedia.org/wiki/~~Dengue_virus_4 Dengue virus 4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [https://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.232Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3uc0\|3uc0]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [https://pdbe.org/3uaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [https://www.ebi.ac.uk/pdbsum/3uaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uaj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [https://pdbe.org/3uaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [https://www.ebi.ac.uk/pdbsum/3uaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uaj ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/POLG_DEN4D POLG_DEN4D] Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (By similarity). prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity). Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity). Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity). Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential). Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity). Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity). Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the NS3 helicase (By similarity). Peptide 2k functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter (By similarity). Non-structural protein 4B inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway (By similarity).
-	~~The four serotypes of dengue virus (DENV~~-1 to ~~-4) cause~~ the ~~most important emerging viral disease~~. ~~Protein~~ E~~, the principal viral~~ envelope ~~glycoprotein, mediates~~ fusion of the viral ~~and endosomal membranes during virus entry and is~~ the ~~target~~ of ~~neutralizing antibodies~~. ~~However~~, the ~~epitopes of strongly neutralizing human antibodies have not been described despite their importance to vaccine development~~. ~~The chimpanzee Mab 5H2 potently neutralizes DENV-4 by~~ binding to ~~domain I of E~~. ~~The crystal structure of Fab 5H2 bound to~~ E ~~from DENV-4 shows that antibody binding prevents formation of the fusogenic hairpin conformation of E, which together with~~ in~~-vitro assays, demonstrates that 5H2 neutralizes by blocking~~ membrane fusion ~~in the endosome~~. ~~Furthermore~~, ~~we show that human sera from patients recovering~~ from ~~DENV~~-~~4 infection contain antibodies that bind to~~ the ~~5H2 epitope region on domain I~~. This ~~study, thus, provides new information~~ and ~~tools for effective vaccine design to prevent~~ dengue ~~disease~~.	+
-		+
-	~~Structural insights into~~ the ~~neutralization mechanism~~ of ~~a higher primate antibody against dengue virus~~.,~~Cockburn JJ~~, ~~Navarro Sanchez ME~~, ~~Goncalvez AP~~, ~~Zaitseva E~~, ~~Stura EA~~, ~~Kikuti CM, Duquerroy S, Dussart P~~, ~~Chernomordik LV, Lai CJ, Rey FA EMBO J~~. ~~2011 Dec 2~~. ~~doi: 10~~.~~1038/emboj~~.~~2011~~.~~439. PMID:22139356<ref>PMID:22139356<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®~~, ~~a database~~ of the U.S. ~~National Library~~ of ~~Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe~~-~~citations 3uaj" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]		*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chimpansee troglodytes]]
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cockburn, J J.B~~]]	+	[[Category: Pan troglodytes]]
-	[[Category: ~~Navarro-Sanchez, M E~~]]	+	[[Category: Cockburn JJB]]
-	[[Category: ~~Rey, F A~~]]	+	[[Category: Navarro-Sanchez ME]]
-	[[Category: ~~Stura, E A~~]]	+	[[Category: Rey FA]]
-	[[Category: ~~Dengue antibody membrane fusion]]~~	+	[[Category: Stura EA]]
-	~~[[Category: Viral protein-immune system complex~~]]	+

OCA at 06:05, 13 July 2022

OCA — Wed, 13 Jul 2022 06:05:14 GMT

←Older revision		Revision as of 06:05, 13 July 2022
Line 1:		Line 1:

	==Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2==		==Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2==
-	<StructureSection load='3uaj' size='340' side='right' caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>	+	<StructureSection load='3uaj' size='340' side='right'caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chimpansee_troglodytes Chimpansee troglodytes] and [~~http~~://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chimpansee_troglodytes Chimpansee troglodytes] and [https://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uc0\|3uc0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uc0\|3uc0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [~~http~~://pdbe.org/3uaj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uaj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uaj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [https://pdbe.org/3uaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [https://www.ebi.ac.uk/pdbsum/3uaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uaj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	[[Category: Chimpansee troglodytes]]		[[Category: Chimpansee troglodytes]]
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
		+	[[Category: Large Structures]]
	[[Category: Cockburn, J J.B]]		[[Category: Cockburn, J J.B]]
	[[Category: Navarro-Sanchez, M E]]		[[Category: Navarro-Sanchez, M E]]

OCA at 06:57, 5 August 2016

OCA — Fri, 05 Aug 2016 06:57:41 GMT

←Older revision		Revision as of 06:57, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2==		==Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2==
	<StructureSection load='3uaj' size='340' side='right' caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>		<StructureSection load='3uaj' size='340' side='right' caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dengue_virus_4 Dengue virus 4~~] and [http://en.wikipedia.org/wiki/~~Pan_troglodytes Pan troglodytes~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chimpansee_troglodytes Chimpansee troglodytes] and [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uc0\|3uc0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uc0\|3uc0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [http://www.ebi.ac.uk/pdbsum/3uaj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [http://pdbe.org/3uaj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [http://www.ebi.ac.uk/pdbsum/3uaj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uaj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uaj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Monoclonal Antibody~~\|~~Monoclonal Antibody~~]]	+	*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chimpansee troglodytes]]
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
-	[[Category: Pan troglodytes]]
	[[Category: Cockburn, J J.B]]		[[Category: Cockburn, J J.B]]
	[[Category: Navarro-Sanchez, M E]]		[[Category: Navarro-Sanchez, M E]]

OCA at 16:43, 9 December 2014

OCA — Tue, 09 Dec 2014 16:43:54 GMT

←Older revision		Revision as of 16:43, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3uaj.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2==
		+	<StructureSection load='3uaj' size='340' side='right' caption='[[3uaj]], [[Resolution\|resolution]] 3.23Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UAJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uc0\|3uc0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">envelope ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11070 Dengue virus 4])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uaj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uaj RCSB], [http://www.ebi.ac.uk/pdbsum/3uaj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The four serotypes of dengue virus (DENV-1 to -4) cause the most important emerging viral disease. Protein E, the principal viral envelope glycoprotein, mediates fusion of the viral and endosomal membranes during virus entry and is the target of neutralizing antibodies. However, the epitopes of strongly neutralizing human antibodies have not been described despite their importance to vaccine development. The chimpanzee Mab 5H2 potently neutralizes DENV-4 by binding to domain I of E. The crystal structure of Fab 5H2 bound to E from DENV-4 shows that antibody binding prevents formation of the fusogenic hairpin conformation of E, which together with in-vitro assays, demonstrates that 5H2 neutralizes by blocking membrane fusion in the endosome. Furthermore, we show that human sera from patients recovering from DENV-4 infection contain antibodies that bind to the 5H2 epitope region on domain I. This study, thus, provides new information and tools for effective vaccine design to prevent dengue disease.

-	~~{{STRUCTURE_3uaj\| PDB=3uaj \| SCENE= }}~~	+	Structural insights into the neutralization mechanism of a higher primate antibody against dengue virus.,Cockburn JJ, Navarro Sanchez ME, Goncalvez AP, Zaitseva E, Stura EA, Kikuti CM, Duquerroy S, Dussart P, Chernomordik LV, Lai CJ, Rey FA EMBO J. 2011 Dec 2. doi: 10.1038/emboj.2011.439. PMID:22139356<ref>PMID:22139356</ref>

-	~~===Crystal structure~~ of the ~~envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22139356}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3uaj]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en~~.~~wikipedia.org~~/~~wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].~~	+

	==See Also==		==See Also==
	*[[Monoclonal Antibody\|Monoclonal Antibody]]		*[[Monoclonal Antibody\|Monoclonal Antibody]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022139356<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Dengue virus 4]]		[[Category: Dengue virus 4]]
	[[Category: Pan troglodytes]]		[[Category: Pan troglodytes]]
-	[[Category: Cockburn, J J.B.]]	+	[[Category: Cockburn, J J.B]]
-	[[Category: Navarro-Sanchez, M E.]]	+	[[Category: Navarro-Sanchez, M E]]
-	[[Category: Rey, F A.]]	+	[[Category: Rey, F A]]
-	[[Category: Stura, E A.]]	+	[[Category: Stura, E A]]
	[[Category: Dengue antibody membrane fusion]]		[[Category: Dengue antibody membrane fusion]]
	[[Category: Viral protein-immune system complex]]		[[Category: Viral protein-immune system complex]]

OCA at 19:01, 20 October 2012

OCA — Sat, 20 Oct 2012 19:01:19 GMT

←Older revision		Revision as of 19:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3uaj]] is a 6 chain structure ~~of [[Monoclonal Antibody]]~~ with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].	+	[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].

	==See Also==		==See Also==

OCA at 06:01, 27 July 2012

OCA — Fri, 27 Jul 2012 06:01:49 GMT

←Older revision		Revision as of 06:01, 27 July 2012
Line 1:		Line 1:
	[[Image:3uaj.png\|left\|200px]]		[[Image:3uaj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uaj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uaj\| PDB=3uaj \| SCENE= }}		{{STRUCTURE_3uaj\| PDB=3uaj \| SCENE= }}

	===Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2===		===Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22139356}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22139356 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22139356}}		{{ABSTRACT_PUBMED_22139356}}

	==About this Structure==		==About this Structure==
-	[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].	+	[[3uaj]] is a 6 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].
		+
		+	==See Also==
		+	*[[Monoclonal Antibody\|Monoclonal Antibody]]

	==Reference==		==Reference==

OCA at 05:43, 15 February 2012

OCA — Wed, 15 Feb 2012 05:43:38 GMT

←Older revision		Revision as of 05:43, 15 February 2012
Line 1:		Line 1:
-	[[Image:3uaj.~~jpg~~\|left\|200px]]	+	[[Image:3uaj.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dengue_virus_serotype_4~~ Dengue virus ~~serotype~~ 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].	+	[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_4 Dengue virus 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:022139356</ref><references group="xtra"/>		<ref group="xtra">PMID:022139356</ref><references group="xtra"/>
-	[[Category: Dengue virus ~~serotype~~ 4]]	+	[[Category: Dengue virus 4]]
	[[Category: Pan troglodytes]]		[[Category: Pan troglodytes]]
	[[Category: Cockburn, J J.B.]]		[[Category: Cockburn, J J.B.]]

OCA at 11:34, 14 December 2011

OCA — Wed, 14 Dec 2011 11:34:04 GMT

←Older revision		Revision as of 11:34, 14 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uaj.jpg\|left\|200px]]

-	The ~~entry 3uaj~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uaj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uaj\| PDB=3uaj \| SCENE= }}

-	~~Authors: Cockburn, J.J.B., Stura, E.A., Navarro-Sanchez, M.E., Rey, F.A.~~	+	===Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2===

-	~~Description~~: ~~Crystal structure of~~ the ~~envelope glycoprotein ectodomain~~ from ~~dengue~~ virus serotype 4 ~~in complex with the fab fragment of the chimpanzee monoclonal~~ antibody ~~5H2~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22139356}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22139356 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22139356}}
		+
		+	==About this Structure==
		+	[[3uaj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_serotype_4 Dengue virus serotype 4] and [http://en.wikipedia.org/wiki/Pan_troglodytes Pan troglodytes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UAJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022139356</ref><references group="xtra"/>
		+	[[Category: Dengue virus serotype 4]]
		+	[[Category: Pan troglodytes]]
		+	[[Category: Cockburn, J J.B.]]
		+	[[Category: Navarro-Sanchez, M E.]]
		+	[[Category: Rey, F A.]]
		+	[[Category: Stura, E A.]]
		+	[[Category: Dengue antibody membrane fusion]]
		+	[[Category: Viral protein-immune system complex]]

OCA at 11:51, 16 November 2011

OCA — Wed, 16 Nov 2011 11:51:22 GMT

←Older revision		Revision as of 11:51, 16 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uaj is ON HOLD	+	The entry 3uaj is ON HOLD until Paper Publication

	Authors: Cockburn, J.J.B., Stura, E.A., Navarro-Sanchez, M.E., Rey, F.A.		Authors: Cockburn, J.J.B., Stura, E.A., Navarro-Sanchez, M.E., Rey, F.A.

	Description: Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2		Description: Crystal structure of the envelope glycoprotein ectodomain from dengue virus serotype 4 in complex with the fab fragment of the chimpanzee monoclonal antibody 5H2

OCA: Protected "3uaj" [edit=sysop:move=sysop]

OCA — Wed, 02 Nov 2011 07:59:33 GMT

Protected "3uaj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:59, 2 November 2011