3ucj - Revision history

OCA at 13:54, 14 March 2024

OCA — Thu, 14 Mar 2024 13:54:42 GMT

←Older revision		Revision as of 13:54, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3ucj' size='340' side='right'caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3ucj' size='340' side='right'caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccomyxa_sp.~~_(strain_pa)~~ Coccomyxa sp. ~~(strain pa)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccomyxa_sp._PA Coccomyxa sp. PA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [https://pdbe.org/3ucj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [https://www.ebi.ac.uk/pdbsum/3ucj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ucj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [https://pdbe.org/3ucj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [https://www.ebi.ac.uk/pdbsum/3ucj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ucj ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q96554_9CHLO Q96554_9CHLO]
-	The beta-class carbonic anhydrases (beta-CAs) are widely distributed among lower eukaryotes, prokaryotes, archaea, and plants. Like all CAs, the beta-enzymes catalyze an important physiological reaction, namely the interconversion between carbon dioxide and bicarbonate. In plants the enzyme plays an important role in carbon fixation and metabolism. To further explore the structure-function relationship of beta-CA, we have determined the crystal structures of the photoautotroph unicellular green alga Coccomyxa beta-CA in complex with five different inhibitors: acetazolamide, thiocyanate, azide, iodide, and phosphate ions. The tetrameric Coccomyxa beta-CA structure is similar to other beta-CAs but it has a 15 amino acid extension in the C-terminal end, which stabilizes the tetramer by strengthening the interface. Four of the five inhibitors bind in a manner similar to what is found in complexes with alpha-type CAs. Iodide ions, however, make contact to the zinc ion via a zinc-bound water molecule or hydroxide ion--a type of binding mode not previously observed in any CA. Binding of inhibitors to Coccomyxa beta-CA is mediated by side-chain movements of the conserved residue Tyr-88, extending the width of the active site cavity with 1.5-1.8 A. Structural analysis and comparisons with other alpha- and beta-class members suggest a catalytic mechanism in which the movements of Tyr-88 are important for the CO(2)-HCO(3)(-) interconversion, whereas a structurally conserved water molecule that bridges residues Tyr-88 and Gln-38, seems important for proton transfer, linking water molecules from the zinc-bound water to His-92 and buffer molecules.	+
-		+
-	Structural studies of beta-carbonic anhydrase from the green alga Coccomyxa: inhibitor complexes with anions and acetazolamide.,Huang S, Hainzl T, Grundstrom C, Forsman C, Samuelsson G, Sauer-Eriksson AE PLoS One. 2011;6(12):e28458. Epub 2011 Dec 5. PMID:22162771<ref>PMID:22162771</~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3ucj" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]		*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Carbonate dehydratase~~]]	+	[[Category: Coccomyxa sp. PA]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hainzl, T]]	+	[[Category: Hainzl T]]
-	[[Category: Huang, S]]	+	[[Category: Huang S]]
-	[[Category: Sauer-Eriksson~~, A E]]~~	+	[[Category: Sauer-Eriksson AE]]
-	~~[[Category: Alpha/beta]]~~	+
-	~~[[Category: Lyase-lyase inhibitor complex]]~~	+
-	~~[[Category: Strand exchange~~]]	+

OCA at 06:07, 13 July 2022

OCA — Wed, 13 Jul 2022 06:07:40 GMT

←Older revision		Revision as of 06:07, 13 July 2022
Line 3:		Line 3:
	<StructureSection load='3ucj' size='340' side='right'caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3ucj' size='340' side='right'caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Coccomyxa_sp._(strain_pa) Coccomyxa sp. (strain pa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccomyxa_sp._(strain_pa) Coccomyxa sp. (strain pa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [~~http~~://pdbe.org/3ucj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ucj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ucj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [https://pdbe.org/3ucj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [https://www.ebi.ac.uk/pdbsum/3ucj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ucj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:43, 17 July 2019

OCA — Wed, 17 Jul 2019 11:43:00 GMT

←Older revision		Revision as of 11:43, 17 July 2019
Line 1:		Line 1:

	==Coccomyxa beta-carbonic anhydrase in complex with acetazolamide==		==Coccomyxa beta-carbonic anhydrase in complex with acetazolamide==
-	<StructureSection load='3ucj' size='340' side='right' caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3ucj' size='340' side='right'caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._(strain_pa) Coccomyxa sp. (strain pa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._(strain_pa) Coccomyxa sp. (strain pa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Carbonic anhydrase\|Carbonic anhydrase]]	+	*[[Carbonic anhydrase 3D structures\|Carbonic anhydrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
		+	[[Category: Large Structures]]
	[[Category: Hainzl, T]]		[[Category: Hainzl, T]]
	[[Category: Huang, S]]		[[Category: Huang, S]]

OCA at 19:06, 10 December 2016

OCA — Sat, 10 Dec 2016 19:06:46 GMT

←Older revision		Revision as of 19:06, 10 December 2016
Line 1:		Line 1:
		+
	==Coccomyxa beta-carbonic anhydrase in complex with acetazolamide==		==Coccomyxa beta-carbonic anhydrase in complex with acetazolamide==
	<StructureSection load='3ucj' size='340' side='right' caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3ucj' size='340' side='right' caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp.~~_pa~~ Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._(strain_pa) Coccomyxa sp. (strain pa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [http://www.ebi.ac.uk/pdbsum/3ucj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [http://pdbe.org/3ucj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [http://www.ebi.ac.uk/pdbsum/3ucj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ucj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ucj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
-	[[Category: Coccomyxa sp. pa]]
	[[Category: Hainzl, T]]		[[Category: Hainzl, T]]
	[[Category: Huang, S]]		[[Category: Huang, S]]

OCA at 17:39, 9 December 2014

OCA — Tue, 09 Dec 2014 17:39:45 GMT

←Older revision		Revision as of 17:39, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3ucj.~~png~~\|~~left~~\|~~200px~~]]	+	==Coccomyxa beta-carbonic anhydrase in complex with acetazolamide==
		+	<StructureSection load='3ucj' size='340' side='right' caption='[[3ucj]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UCJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZM:5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE'>AZM</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uck\|3uck]], [[3ucm\|3ucm]], [[3ucn\|3ucn]], [[3uco\|3uco]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ucj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ucj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ucj RCSB], [http://www.ebi.ac.uk/pdbsum/3ucj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The beta-class carbonic anhydrases (beta-CAs) are widely distributed among lower eukaryotes, prokaryotes, archaea, and plants. Like all CAs, the beta-enzymes catalyze an important physiological reaction, namely the interconversion between carbon dioxide and bicarbonate. In plants the enzyme plays an important role in carbon fixation and metabolism. To further explore the structure-function relationship of beta-CA, we have determined the crystal structures of the photoautotroph unicellular green alga Coccomyxa beta-CA in complex with five different inhibitors: acetazolamide, thiocyanate, azide, iodide, and phosphate ions. The tetrameric Coccomyxa beta-CA structure is similar to other beta-CAs but it has a 15 amino acid extension in the C-terminal end, which stabilizes the tetramer by strengthening the interface. Four of the five inhibitors bind in a manner similar to what is found in complexes with alpha-type CAs. Iodide ions, however, make contact to the zinc ion via a zinc-bound water molecule or hydroxide ion--a type of binding mode not previously observed in any CA. Binding of inhibitors to Coccomyxa beta-CA is mediated by side-chain movements of the conserved residue Tyr-88, extending the width of the active site cavity with 1.5-1.8 A. Structural analysis and comparisons with other alpha- and beta-class members suggest a catalytic mechanism in which the movements of Tyr-88 are important for the CO(2)-HCO(3)(-) interconversion, whereas a structurally conserved water molecule that bridges residues Tyr-88 and Gln-38, seems important for proton transfer, linking water molecules from the zinc-bound water to His-92 and buffer molecules.

-	~~{{STRUCTURE_3ucj\| PDB=3ucj \| SCENE= }}~~	+	Structural studies of beta-carbonic anhydrase from the green alga Coccomyxa: inhibitor complexes with anions and acetazolamide.,Huang S, Hainzl T, Grundstrom C, Forsman C, Samuelsson G, Sauer-Eriksson AE PLoS One. 2011;6(12):e28458. Epub 2011 Dec 5. PMID:22162771<ref>PMID:22162771</ref>

-	~~===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22162771}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ucj]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Coccomyxa_sp~~.~~_pa Coccomyxa sp. pa]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].~~	+

	==See Also==		==See Also==
	*[[Carbonic anhydrase\|Carbonic anhydrase]]		*[[Carbonic anhydrase\|Carbonic anhydrase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022162771<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Coccomyxa sp. pa]]		[[Category: Coccomyxa sp. pa]]
-	[[Category: Hainzl, T.]]	+	[[Category: Hainzl, T]]
-	[[Category: Huang, S.]]	+	[[Category: Huang, S]]
-	[[Category: Sauer-Eriksson, A E.]]	+	[[Category: Sauer-Eriksson, A E]]
	[[Category: Alpha/beta]]		[[Category: Alpha/beta]]
	[[Category: Lyase-lyase inhibitor complex]]		[[Category: Lyase-lyase inhibitor complex]]
	[[Category: Strand exchange]]		[[Category: Strand exchange]]

OCA at 08:59, 20 October 2012

OCA — Sat, 20 Oct 2012 08:59:02 GMT

←Older revision		Revision as of 08:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3ucj]] is a 2 chain structure ~~of [[Carbonic anhydrase]]~~ with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].	+	[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].

	==See Also==		==See Also==

OCA at 21:24, 25 July 2012

OCA — Wed, 25 Jul 2012 21:24:07 GMT

←Older revision		Revision as of 21:24, 25 July 2012
Line 1:		Line 1:
	[[Image:3ucj.png\|left\|200px]]		[[Image:3ucj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ucj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ucj\| PDB=3ucj \| SCENE= }}		{{STRUCTURE_3ucj\| PDB=3ucj \| SCENE= }}

	===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===		===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22162771}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22162771 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22162771}}		{{ABSTRACT_PUBMED_22162771}}

	==About this Structure==		==About this Structure==
-	[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].	+	[[3ucj]] is a 2 chain structure of [[Carbonic anhydrase]] with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].
		+
		+	==See Also==
		+	*[[Carbonic anhydrase\|Carbonic anhydrase]]

	==Reference==		==Reference==

OCA at 06:50, 28 March 2012

OCA — Wed, 28 Mar 2012 06:50:15 GMT

←Older revision		Revision as of 06:50, 28 March 2012
Line 1:		Line 1:
-	[[Image:3ucj.~~jpg~~\|left\|200px]]	+	[[Image:3ucj.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===		===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22162771}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22162771 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22162771}}

	==About this Structure==		==About this Structure==
	[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].		[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022162771</ref><references group="xtra"/>
	[[Category: Carbonate dehydratase]]		[[Category: Carbonate dehydratase]]
	[[Category: Coccomyxa sp. pa]]		[[Category: Coccomyxa sp. pa]]

OCA at 19:19, 23 November 2011

OCA — Wed, 23 Nov 2011 19:19:36 GMT

←Older revision		Revision as of 19:19, 23 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ucj.jpg\|left\|200px]]

-	The ~~entry 3ucj~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ucj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ucj\| PDB=3ucj \| SCENE= }}

-	~~Authors: Huang, S., Hainzl, T., Sauer~~-~~Eriksson, A.E.~~	+	===Coccomyxa beta-carbonic anhydrase in complex with acetazolamide===

-	~~Description~~: Coccomyxa beta-~~carbonic anhydrase in~~ complex ~~with acetazolamide~~	+
		+	==About this Structure==
		+	[[3ucj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccomyxa_sp._pa Coccomyxa sp. pa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UCJ OCA].
		+	[[Category: Carbonate dehydratase]]
		+	[[Category: Coccomyxa sp. pa]]
		+	[[Category: Hainzl, T.]]
		+	[[Category: Huang, S.]]
		+	[[Category: Sauer-Eriksson, A E.]]
		+	[[Category: Alpha/beta]]
		+	[[Category: Lyase-lyase inhibitor complex]]
		+	[[Category: Strand exchange]]

OCA: Protected "3ucj" [edit=sysop:move=sysop]

OCA — Wed, 02 Nov 2011 08:01:39 GMT

Protected "3ucj" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:01, 2 November 2011