3uet - Revision history

OCA at 17:35, 1 November 2023

OCA — Wed, 01 Nov 2023 17:35:41 GMT

←Older revision		Revision as of 17:35, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3uet' size='340' side='right'caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3uet' size='340' side='right'caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bifls Bifls~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis_ATCC_15697_=_JCM_1222_=_DSM_20088 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UET FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=~~FUC~~:~~ALPHA~~-L-~~FUCOSE~~'>~~FUC~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA-D-GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3mo4\|3mo4]]~~, ~~[[3ues\|3ues]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900129:Lewis+A+antigen,+beta+anomer'>PRD_900129</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">BLIJ_2413~~, ~~Blon_2236 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [https://pdbe.org/3uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [https://www.ebi.ac.uk/pdbsum/3uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [https://pdbe.org/3uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [https://www.ebi.ac.uk/pdbsum/3uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B7GNN8_BIFLS B7GNN8_BIFLS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~1,3-alpha-L-fucosidase]]~~	+	[[Category: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088]]
-	~~[[Category: Bifls~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ashida, H]]	+	[[Category: Ashida H]]
-	[[Category: Fushinobu, S]]	+	[[Category: Fushinobu S]]
-	[[Category: Hidaka, M]]	+	[[Category: Hidaka M]]
-	[[Category: Honda, Y]]	+	[[Category: Honda Y]]
-	[[Category: Katayama, T]]	+	[[Category: Katayama T]]
-	[[Category: Kitaoka, M]]	+	[[Category: Kitaoka M]]
-	[[Category: Kumagai, H]]	+	[[Category: Kumagai H]]
-	[[Category: Sakurama, H]]	+	[[Category: Sakurama H]]
-	[[Category: Yamamoto, K]]	+	[[Category: Yamamoto K]]
-	[[Category: Yoshida, E]]	+	[[Category: Yoshida E]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 07:36, 20 July 2022

OCA — Wed, 20 Jul 2022 07:36:23 GMT

←Older revision		Revision as of 07:36, 20 July 2022
Line 3:		Line 3:
	<StructureSection load='3uet' size='340' side='right'caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3uet' size='340' side='right'caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3UET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UET FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3mo4\|3mo4]], [[3ues\|3ues]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [~~http~~://pdbe.org/3uet PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uet PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [https://pdbe.org/3uet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [https://www.ebi.ac.uk/pdbsum/3uet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:38, 5 August 2020

OCA — Wed, 05 Aug 2020 05:38:27 GMT

←Older revision		Revision as of 05:38, 5 August 2020
Line 1:		Line 1:

	==Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II==		==Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II==
-	<StructureSection load='3uet' size='340' side='right' caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3uet' size='340' side='right'caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3UET FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=~~FUC~~:~~ALPHA-L-FUCOSE~~'>~~FUC~~</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [http://pdbe.org/3uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [http://www.ebi.ac.uk/pdbsum/3uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [http://pdbe.org/3uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [http://www.ebi.ac.uk/pdbsum/3uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: 1,3-alpha-L-fucosidase]]		[[Category: 1,3-alpha-L-fucosidase]]
	[[Category: Bifls]]		[[Category: Bifls]]
		+	[[Category: Large Structures]]
	[[Category: Ashida, H]]		[[Category: Ashida, H]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]

OCA at 19:00, 10 December 2016

OCA — Sat, 10 Dec 2016 19:00:27 GMT

←Older revision		Revision as of 19:00, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II==		==Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II==
	<StructureSection load='3uet' size='340' side='right' caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3uet' size='340' side='right' caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bifidobacterium_longum_subsp._infantis_atcc_15697_=_jcm_1222 Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifls Bifls]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UET FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 ~~Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 BIFLS])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [http://www.ebi.ac.uk/pdbsum/3uet PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [http://pdbe.org/3uet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [http://www.ebi.ac.uk/pdbsum/3uet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uet ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uet" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: 1,3-alpha-L-fucosidase]]		[[Category: 1,3-alpha-L-fucosidase]]
-	[[Category: ~~Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222~~]]	+	[[Category: Bifls]]
	[[Category: Ashida, H]]		[[Category: Ashida, H]]
	[[Category: Fushinobu, S]]		[[Category: Fushinobu, S]]

OCA at 06:50, 15 February 2015

OCA — Sun, 15 Feb 2015 06:50:39 GMT

←Older revision		Revision as of 06:50, 15 February 2015
Line 1:		Line 1:
-	[[~~Image:~~3uet.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II==
		+	<StructureSection load='3uet' size='340' side='right' caption='[[3uet]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis_atcc_15697_=_jcm_1222 Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UET FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mo4\|3mo4]], [[3ues\|3ues]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLIJ_2413, Blon_2236 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391904 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,3-alpha-L-fucosidase 1,3-alpha-L-fucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.111 3.2.1.111] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uet OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uet RCSB], [http://www.ebi.ac.uk/pdbsum/3uet PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-L-fucosyl residues attached at the non-reducing ends of glycoconjugates constitute histo-blood group antigens Lewis (Le) and ABO and play fundamental roles in various biological processes. Therefore, establishing a method for synthesizing the antigens is important for functional glycomics studies. However, regiospecific synthesis of glycosyl linkages, especially alpha-L-fucosyl linkages, is quite difficult to control both by chemists and enzymologists. Here, we generated an alpha-L-fucosynthase that specifically introduces Le(a) and Le(x) antigens into the type-1 and type-2 chains, respectively; i.e. the enzyme specifically accepts the disaccharide structures (Galbeta1-3/4GlcNAc) at the non-reducing ends and attaches a Fuc residue via an alpha-(1,4/3)-linkage to the GlcNAc. X-ray crystallographic studies revealed the structural basis of this strict regio- and acceptor specificity, which includes the induced fit movement of the catalytically important residues, and the difference between the active site structures of 1,3-1,4-alpha-L-fucosidase (EC 3.2.1.111) and alpha-L-fucosidase (EC 3.2.1.51) in glycoside hydrolase family 29. The glycosynthase developed in this study should serve as a potentially powerful tool to specifically introduce the Le(a/x) epitopes onto labile glycoconjugates including glycoproteins. Mining glycosidases with strict specificity may represent the most efficient route to the specific synthesis of glycosidic bonds.

-	~~{{STRUCTURE_3uet\| PDB=3uet \| SCENE= }}~~	+	1,3-1,4-alpha-L-fucosynthase that specifically introduces Lewis a/x antigens into type-1/2 chains.,Sakurama H, Fushinobu S, Hidaka M, Yoshida E, Honda Y, Ashida H, Kitaoka M, Kumagai H, Yamamoto K, Katayama T J Biol Chem. 2012 May 11;287(20):16709-19. Epub 2012 Mar 26. PMID:22451675<ref>PMID:22451675</ref>

-	~~===Crystal structure~~ of ~~alpha-1,3/4-fucosidase from Bifidobacterium longum subsp~~. ~~infantis D172A~~/~~E217A mutant complexed with lacto-N-fucopentaose II===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22451675}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3uet]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis_atcc_15697_=_jcm_1222 Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA].	+	</StructureSection>
	[[Category: 1,3-alpha-L-fucosidase]]		[[Category: 1,3-alpha-L-fucosidase]]
	[[Category: Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]]		[[Category: Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]]
-	[[Category: Ashida, H.]]	+	[[Category: Ashida, H]]
-	[[Category: Fushinobu, S.]]	+	[[Category: Fushinobu, S]]
-	[[Category: Hidaka, M.]]	+	[[Category: Hidaka, M]]
-	[[Category: Honda, Y.]]	+	[[Category: Honda, Y]]
-	[[Category: Katayama, T.]]	+	[[Category: Katayama, T]]
-	[[Category: Kitaoka, M.]]	+	[[Category: Kitaoka, M]]
-	[[Category: Kumagai, H.]]	+	[[Category: Kumagai, H]]
-	[[Category: Sakurama, H.]]	+	[[Category: Sakurama, H]]
-	[[Category: Yamamoto, K.]]	+	[[Category: Yamamoto, K]]
-	[[Category: Yoshida, E.]]	+	[[Category: Yoshida, E]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 21:15, 12 December 2012

OCA — Wed, 12 Dec 2012 21:15:32 GMT

←Older revision		Revision as of 21:15, 12 December 2012
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-	[[Image:3uet.~~jpg~~\|left\|200px]]	+	[[Image:3uet.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uet", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uet\| PDB=3uet \| SCENE= }}		{{STRUCTURE_3uet\| PDB=3uet \| SCENE= }}

	===Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II===		===Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II===

		+	{{ABSTRACT_PUBMED_22451675}}

	==About this Structure==		==About this Structure==
-	[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp.~~_infantis~~ Bifidobacterium longum subsp. infantis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA].	+	[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis_atcc_15697_=_jcm_1222 Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA].
	[[Category: 1,3-alpha-L-fucosidase]]		[[Category: 1,3-alpha-L-fucosidase]]
-	[[Category: Bifidobacterium longum subsp. infantis]]	+	[[Category: Bifidobacterium longum subsp. infantis atcc 15697 = jcm 1222]]
	[[Category: Ashida, H.]]		[[Category: Ashida, H.]]
	[[Category: Fushinobu, S.]]		[[Category: Fushinobu, S.]]

OCA at 06:16, 4 April 2012

OCA — Wed, 04 Apr 2012 06:16:54 GMT

←Older revision		Revision as of 06:16, 4 April 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3uet.jpg\|left\|200px]]

-	The ~~entry 3uet~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uet", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uet\| PDB=3uet \| SCENE= }}

-	~~Authors: Sakurama~~, ~~H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumagai, H~~.	+	===Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II===

-	~~Description: Crystal~~ structure ~~of alpha~~-1,3/4-fucosidase ~~from~~ Bifidobacterium longum subsp. infantis ~~D172A/E217A mutant complexed with lacto-N-fucopentaose II~~	+
		+	==About this Structure==
		+	[[3uet]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_longum_subsp._infantis Bifidobacterium longum subsp. infantis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UET OCA].
		+	[[Category: 1,3-alpha-L-fucosidase]]
		+	[[Category: Bifidobacterium longum subsp. infantis]]
		+	[[Category: Ashida, H.]]
		+	[[Category: Fushinobu, S.]]
		+	[[Category: Hidaka, M.]]
		+	[[Category: Honda, Y.]]
		+	[[Category: Katayama, T.]]
		+	[[Category: Kitaoka, M.]]
		+	[[Category: Kumagai, H.]]
		+	[[Category: Sakurama, H.]]
		+	[[Category: Yamamoto, K.]]
		+	[[Category: Yoshida, E.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Tim barrel]]

OCA at 05:51, 30 November 2011

OCA — Wed, 30 Nov 2011 05:51:10 GMT

←Older revision		Revision as of 05:51, 30 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uet is ON HOLD	+	The entry 3uet is ON HOLD until Paper Publication

	Authors: Sakurama, H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumagai, H.		Authors: Sakurama, H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumagai, H.

-	Description: Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis complexed with lacto-N-fucopentaose II	+	Description: Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis D172A/E217A mutant complexed with lacto-N-fucopentaose II

OCA: Protected "3uet" [edit=sysop:move=sysop]

OCA — Wed, 09 Nov 2011 11:17:33 GMT

Protected "3uet" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:17, 9 November 2011

OCA: New page: '''Unreleased structure''' The entry 3uet is ON HOLD Authors: Sakurama, H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumag...

OCA — Wed, 09 Nov 2011 11:17:32 GMT

New page: '''Unreleased structure''' The entry 3uet is ON HOLD Authors: Sakurama, H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumag...

New page

'''Unreleased structure'''

The entry 3uet is ON HOLD

Authors: Sakurama, H., Fushinobu, S., Yoshida, E., Honda, Y., Hidaka, M., Ashida, H., Kitaoka, M., Katayama, T., Yamamoto, K., Kumagai, H.

Description: Crystal structure of alpha-1,3/4-fucosidase from Bifidobacterium longum subsp. infantis complexed with lacto-N-fucopentaose II