3ug6 - Revision history

OCA at 10:23, 1 March 2024

OCA — Fri, 01 Mar 2024 10:23:14 GMT

←Older revision		Revision as of 10:23, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3ug6' size='340' side='right'caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='3ug6' size='340' side='right'caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Metja Metja~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3ug7\|3ug7]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~MJ1142 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=243232 METJA])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [https://pdbe.org/3ug6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ug6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ug6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [https://pdbe.org/3ug6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ug6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ug6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARSA_METJA ARSA_METJA]] Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity).	+	[https://www.uniprot.org/uniprot/ARSA_METJA ARSA_METJA] Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Efficient delivery of membrane proteins is a critical cellular process. The recently elucidated GET (Guided Entry of TA proteins) pathway is responsible for the targeted delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum. The central player is the ATPase Get3, which in its free form exists as a dimer. Biochemical evidence suggests a role for a tetramer of Get3. Here, we present the first crystal structure of an archaeal Get3 homologue that exists as a tetramer and is capable of TA protein binding. The tetramer generates a hydrophobic chamber that we propose binds the TA protein. We use small-angle X-ray scattering to provide the first structural information of a fungal Get3/TA protein complex showing that the overall molecular envelope is consistent with the archaeal tetramer structure. Moreover, we show that this fungal tetramer complex is capable of TA insertion. This allows us to suggest a model where a tetramer of Get3 sequesters a TA protein during targeting to the membrane.	+
-		+
-	Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.,Suloway CJ, Rome ME, Clemons WM Jr EMBO J. 2011 Nov 29. doi: 10.1038/emboj.2011.433. PMID:22124326<ref>PMID:22124326</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ug6" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arsenite-transporting ATPase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Metja~~]]	+	[[Category: Methanocaldococcus jannaschii DSM 2661]]
-	[[Category: Clemons~~, W M~~]]	+	[[Category: Clemons Jr WM]]
-	[[Category: Rome~~, M E~~]]	+	[[Category: Rome ME]]
-	[[Category: Suloway~~, C J.M]]~~	+	[[Category: Suloway CJM]]
-	~~[[Category: Arsa]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Get3]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Protein targeting]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Tail-anchored]]~~	+
-	~~[[Category: Targeting factor]]~~	+
-	~~[[Category: Trc40~~]]	+

OCA at 06:11, 13 July 2022

OCA — Wed, 13 Jul 2022 06:11:55 GMT

←Older revision		Revision as of 06:11, 13 July 2022
Line 1:		Line 1:

	==Crystal Structure of Get3 from Methanocaldococcus jannaschii==		==Crystal Structure of Get3 from Methanocaldococcus jannaschii==
-	<StructureSection load='3ug6' size='340' side='right' caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='3ug6' size='340' side='right'caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ug7\|3ug7]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [~~http~~://pdbe.org/3ug6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ug6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ug6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [https://pdbe.org/3ug6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ug6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ug6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARSA_METJA ARSA_METJA]] Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity).	+	[[https://www.uniprot.org/uniprot/ARSA_METJA ARSA_METJA]] Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
		+	[[Category: Large Structures]]
	[[Category: Metja]]		[[Category: Metja]]
	[[Category: Clemons, W M]]		[[Category: Clemons, W M]]

OCA at 16:46, 11 August 2016

OCA — Thu, 11 Aug 2016 16:46:39 GMT

←Older revision		Revision as of 16:46, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Get3 from Methanocaldococcus jannaschii==		==Crystal Structure of Get3 from Methanocaldococcus jannaschii==
	<StructureSection load='3ug6' size='340' side='right' caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='3ug6' size='340' side='right' caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methanocaldococcus_jannaschii Methanocaldococcus jannaschii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~2190 Methanocaldococcus jannaschii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243232 METJA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ug6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [http://pdbe.org/3ug6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ug6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ug6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARSA_METJA ARSA_METJA]] Anion-transporting ATPase. Catalyzes the extrusion of arsenite (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ug6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
-	[[Category: ~~Methanocaldococcus jannaschii~~]]	+	[[Category: Metja]]
	[[Category: Clemons, W M]]		[[Category: Clemons, W M]]
	[[Category: Rome, M E]]		[[Category: Rome, M E]]

OCA at 10:12, 4 January 2015

OCA — Sun, 04 Jan 2015 10:12:36 GMT

←Older revision		Revision as of 10:12, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ug6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ug6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.,Suloway CJ, Rome ME, Clemons WM Jr EMBO J. 2011 Nov 29. doi: 10.1038/emboj.2011.433. PMID:22124326<ref>PMID:22124326</ref>		Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.,Suloway CJ, Rome ME, Clemons WM Jr EMBO J. 2011 Nov 29. doi: 10.1038/emboj.2011.433. PMID:22124326<ref>PMID:22124326</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 23:
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Methanocaldococcus jannaschii]]		[[Category: Methanocaldococcus jannaschii]]
-	[[Category: Clemons, W M.]]	+	[[Category: Clemons, W M]]
-	[[Category: Rome, M E.]]	+	[[Category: Rome, M E]]
-	[[Category: Suloway, C J.M.]]	+	[[Category: Suloway, C J.M]]
	[[Category: Arsa]]		[[Category: Arsa]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 06:46, 5 June 2014

OCA — Thu, 05 Jun 2014 06:46:49 GMT

←Older revision		Revision as of 06:46, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3ug6.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Get3 from Methanocaldococcus jannaschii==
		+	<StructureSection load='3ug6' size='340' side='right' caption='[[3ug6]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UG6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ug7\|3ug7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MJ1142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ug6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ug6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ug6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ug6 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Efficient delivery of membrane proteins is a critical cellular process. The recently elucidated GET (Guided Entry of TA proteins) pathway is responsible for the targeted delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum. The central player is the ATPase Get3, which in its free form exists as a dimer. Biochemical evidence suggests a role for a tetramer of Get3. Here, we present the first crystal structure of an archaeal Get3 homologue that exists as a tetramer and is capable of TA protein binding. The tetramer generates a hydrophobic chamber that we propose binds the TA protein. We use small-angle X-ray scattering to provide the first structural information of a fungal Get3/TA protein complex showing that the overall molecular envelope is consistent with the archaeal tetramer structure. Moreover, we show that this fungal tetramer complex is capable of TA insertion. This allows us to suggest a model where a tetramer of Get3 sequesters a TA protein during targeting to the membrane.

-	<!--	+	Tail-anchor targeting by a Get3 tetramer: the structure of an archaeal homologue.,Suloway CJ, Rome ME, Clemons WM Jr EMBO J. 2011 Nov 29. doi: 10.1038/emboj.2011.433. PMID:22124326<ref>PMID:22124326</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3ug6", creates~~ the ~~"Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3ug6\| PDB=3ug6 \| SCENE= }}~~	+

-	~~===Crystal Structure~~ of ~~Get3 from Methanocaldococcus jannaschii===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22124326}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22124326 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22124326}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ug6]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022124326<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arsenite-transporting ATPase]]		[[Category: Arsenite-transporting ATPase]]
	[[Category: Methanocaldococcus jannaschii]]		[[Category: Methanocaldococcus jannaschii]]

OCA at 05:46, 11 January 2012

OCA — Wed, 11 Jan 2012 05:46:31 GMT

←Older revision		Revision as of 05:46, 11 January 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3ug6.png\|left\|200px]]

-	The ~~entry 3ug6~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ug6", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ug6\| PDB=3ug6 \| SCENE= }}

-	~~Authors: Suloway, C.J.M., Rome, M.E., Clemons Jr., W.M.~~	+	===Crystal Structure of Get3 from Methanocaldococcus jannaschii===

-	~~Description~~: ~~Crystal~~ Structure ~~of Get3~~ from Methanocaldococcus jannaschii	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22124326}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22124326 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22124326}}
		+
		+	==About this Structure==
		+	[[3ug6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UG6 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022124326</ref><references group="xtra"/>
		+	[[Category: Arsenite-transporting ATPase]]
		+	[[Category: Methanocaldococcus jannaschii]]
		+	[[Category: Clemons, W M.]]
		+	[[Category: Rome, M E.]]
		+	[[Category: Suloway, C J.M.]]
		+	[[Category: Arsa]]
		+	[[Category: Atp-binding]]
		+	[[Category: Atpase]]
		+	[[Category: Get3]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane protein]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Protein targeting]]
		+	[[Category: Protein transport]]
		+	[[Category: Tail-anchored]]
		+	[[Category: Targeting factor]]
		+	[[Category: Trc40]]

OCA: Protected "3ug6" [edit=sysop:move=sysop]

OCA — Wed, 09 Nov 2011 11:18:49 GMT

Protected "3ug6" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:18, 9 November 2011

OCA: New page: '''Unreleased structure''' The entry 3ug6 is ON HOLD Authors: Suloway, C.J.M., Rome, M.E., Clemons Jr., W.M. Description: Crystal Structure of Get3 from Methanocaldococcus jannaschii

OCA — Wed, 09 Nov 2011 11:18:48 GMT

New page: '''Unreleased structure''' The entry 3ug6 is ON HOLD Authors: Suloway, C.J.M., Rome, M.E., Clemons Jr., W.M. Description: Crystal Structure of Get3 from Methanocaldococcus jannaschii

New page

'''Unreleased structure'''

The entry 3ug6 is ON HOLD

Authors: Suloway, C.J.M., Rome, M.E., Clemons Jr., W.M.

Description: Crystal Structure of Get3 from Methanocaldococcus jannaschii