3uje - Revision history

OCA at 14:00, 14 March 2024

OCA — Thu, 14 Mar 2024 14:00:23 GMT

←Older revision		Revision as of 14:00, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uje' size='340' side='right'caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3uje' size='340' side='right'caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UJE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UJE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [https://pdbe.org/3uje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [https://www.ebi.ac.uk/pdbsum/3uje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uje ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [https://pdbe.org/3uje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [https://www.ebi.ac.uk/pdbsum/3uje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uje ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ENOG_HUMAN ENOG_HUMAN]] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).	+	[https://www.uniprot.org/uniprot/ENOG_HUMAN ENOG_HUMAN] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	In the presence of magnesium, enolase catalyzes the dehydration of 2-phospho-d-glycerate (PGA) to phosphoenolpyruvate (PEP) in glycolysis and the reverse reaction in gluconeogensis at comparable rates. The structure of human neuron specific enolase (hNSE) crystals soaked in PGA showed that the enzyme is active in the crystals and produced PEP; conversely soaking in PEP produced PGA. Moreover, the hNSE dimer contains PGA bound in one subunit and PEP or a mixture of PEP and PGA in the other. Crystals soaked in a mixture of competitive inhibitors tartronate semialdehyde phosphate (TSP) and lactic acid phosphate (LAP) showed asymmetry with TSP binding in the same site as PGA and LAP in the PEP site. Kinetic studies showed that the inhibition of NSE by mixtures of TSP and LAP is stronger than predicted for independently acting inhibitors. This indicates that in some cases inhibition of homodimeric enzymes by mixtures of inhibitors ("heteroinhibition") may offer advantages over single inhibitors.	+
-		+
-	Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity.,Qin J, Chai G, Brewer JM, Lovelace LL, Lebioda L J Inorg Biochem. 2012 Jun;111:187-94. Epub 2012 Feb 24. PMID:22437160<ref>PMID:22437160</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Enolase 3D structures\|Enolase 3D structures]]		*[[Enolase 3D structures\|Enolase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphopyruvate hydratase]]~~	+	[[Category: Brewer J]]
-	[[Category: Brewer, J]]	+	[[Category: Chai G]]
-	[[Category: Chai, G]]	+	[[Category: Lebioda L]]
-	[[Category: Lebioda, L]]	+	[[Category: Lovelace L]]
-	[[Category: Lovelace, L]]	+	[[Category: Qin J]]
-	[[Category: Qin, J]]	+
-	~~[[Category: Lyase~~]]	+

OCA at 07:40, 20 July 2022

OCA — Wed, 20 Jul 2022 07:40:10 GMT

←Older revision		Revision as of 07:40, 20 July 2022
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	==Asymmetric complex of human neuron specific enolase-3-PGA/PEP==		==Asymmetric complex of human neuron specific enolase-3-PGA/PEP==
-	<StructureSection load='3uje' size='340' side='right' caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='3uje' size='340' side='right'caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UJE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UJE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [~~http~~://pdbe.org/3uje PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uje PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uje ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [https://pdbe.org/3uje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [https://www.ebi.ac.uk/pdbsum/3uje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uje ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ENOG_HUMAN ENOG_HUMAN]] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).	+	[[https://www.uniprot.org/uniprot/ENOG_HUMAN ENOG_HUMAN]] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Enolase 3D structures\|Enolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Brewer, J]]		[[Category: Brewer, J]]

OCA at 10:06, 8 November 2017

OCA — Wed, 08 Nov 2017 10:06:23 GMT

←Older revision		Revision as of 10:06, 8 November 2017
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Enolase\|Enolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:53, 4 August 2016

OCA — Thu, 04 Aug 2016 09:53:29 GMT

←Older revision		Revision as of 09:53, 4 August 2016
Line 1:		Line 1:
		+
	==Asymmetric complex of human neuron specific enolase-3-PGA/PEP==		==Asymmetric complex of human neuron specific enolase-3-PGA/PEP==
	<StructureSection load='3uje' size='340' side='right' caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3uje' size='340' side='right' caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UJE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UJE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [http://www.ebi.ac.uk/pdbsum/3uje PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [http://pdbe.org/3uje PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [http://www.ebi.ac.uk/pdbsum/3uje PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uje ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uje" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Brewer, J]]		[[Category: Brewer, J]]

OCA at 08:04, 25 December 2014

OCA — Thu, 25 Dec 2014 08:04:38 GMT

←Older revision		Revision as of 08:04, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UJE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UJE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [http://www.ebi.ac.uk/pdbsum/3uje PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [http://www.ebi.ac.uk/pdbsum/3uje PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENOG_HUMAN ENOG_HUMAN]] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 28:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
-	[[Category: Brewer, J.]]	+	[[Category: Brewer, J]]
-	[[Category: Chai, G.]]	+	[[Category: Chai, G]]
-	[[Category: Lebioda, L.]]	+	[[Category: Lebioda, L]]
-	[[Category: Lovelace, L.]]	+	[[Category: Lovelace, L]]
-	[[Category: Qin, J.]]	+	[[Category: Qin, J]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 09:15, 11 June 2014

OCA — Wed, 11 Jun 2014 09:15:02 GMT

←Older revision		Revision as of 09:15, 11 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3uje.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Asymmetric complex of human neuron specific enolase-3-PGA/PEP==
		+	<StructureSection load='3uje' size='340' side='right' caption='[[3uje]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UJE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ujf\|3ujf]], [[3ujr\|3ujr]], [[3ujs\|3ujs]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uje OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uje RCSB], [http://www.ebi.ac.uk/pdbsum/3uje PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the presence of magnesium, enolase catalyzes the dehydration of 2-phospho-d-glycerate (PGA) to phosphoenolpyruvate (PEP) in glycolysis and the reverse reaction in gluconeogensis at comparable rates. The structure of human neuron specific enolase (hNSE) crystals soaked in PGA showed that the enzyme is active in the crystals and produced PEP; conversely soaking in PEP produced PGA. Moreover, the hNSE dimer contains PGA bound in one subunit and PEP or a mixture of PEP and PGA in the other. Crystals soaked in a mixture of competitive inhibitors tartronate semialdehyde phosphate (TSP) and lactic acid phosphate (LAP) showed asymmetry with TSP binding in the same site as PGA and LAP in the PEP site. Kinetic studies showed that the inhibition of NSE by mixtures of TSP and LAP is stronger than predicted for independently acting inhibitors. This indicates that in some cases inhibition of homodimeric enzymes by mixtures of inhibitors ("heteroinhibition") may offer advantages over single inhibitors.

-	~~{{STRUCTURE_3uje\| PDB=3uje \| SCENE= }}~~	+	Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity.,Qin J, Chai G, Brewer JM, Lovelace LL, Lebioda L J Inorg Biochem. 2012 Jun;111:187-94. Epub 2012 Feb 24. PMID:22437160<ref>PMID:22437160</ref>

-	~~===Asymmetric complex~~ of ~~human neuron specific enolase-3-PGA~~/~~PEP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22437160}}~~	+	==See Also==
-		+	*[[Enolase\|Enolase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3uje~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022437160<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]

OCA at 05:57, 22 August 2012

OCA — Wed, 22 Aug 2012 05:57:42 GMT

←Older revision		Revision as of 05:57, 22 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uje.jpg\|left\|200px]]

-	~~The entry~~ 3uje ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3uje\| PDB=3uje \| SCENE= }}

-	~~Authors: Qin, J., Chai, G., Brewer, J., Lovelace, L., Lebioda, L.~~	+	===Asymmetric complex of human neuron specific enolase-3-PGA/PEP===

-	~~Description~~: ~~Asymmetric complex of human neuron specific enolase~~-~~3-PGA~~/~~PEP~~	+	{{ABSTRACT_PUBMED_22437160}}
		+
		+	==About this Structure==
		+	[[3uje]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJE OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022437160</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Phosphopyruvate hydratase]]
		+	[[Category: Brewer, J.]]
		+	[[Category: Chai, G.]]
		+	[[Category: Lebioda, L.]]
		+	[[Category: Lovelace, L.]]
		+	[[Category: Qin, J.]]
		+	[[Category: Lyase]]

OCA at 07:03, 4 July 2012

OCA — Wed, 04 Jul 2012 07:03:25 GMT

←Older revision		Revision as of 07:03, 4 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uje is ON HOLD	+	The entry 3uje is ON HOLD until Paper Publication

	Authors: Qin, J., Chai, G., Brewer, J., Lovelace, L., Lebioda, L.		Authors: Qin, J., Chai, G., Brewer, J., Lovelace, L., Lebioda, L.

	Description: Asymmetric complex of human neuron specific enolase-3-PGA/PEP		Description: Asymmetric complex of human neuron specific enolase-3-PGA/PEP

OCA at 18:37, 23 November 2011

OCA — Wed, 23 Nov 2011 18:37:53 GMT

←Older revision		Revision as of 18:37, 23 November 2011
Line 3:		Line 3:
	The entry 3uje is ON HOLD		The entry 3uje is ON HOLD

-	Authors: Qin, J, Chai, G, Brewer, J, Lovelace, L, Lebioda, L	+	Authors: Qin, J., Chai, G., Brewer, J., Lovelace, L., Lebioda, L.

	Description: Asymmetric complex of human neuron specific enolase-3-PGA/PEP		Description: Asymmetric complex of human neuron specific enolase-3-PGA/PEP

OCA: Protected "3uje" [edit=sysop:move=sysop]

OCA — Wed, 16 Nov 2011 11:55:15 GMT

Protected "3uje" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:55, 16 November 2011