3ump - Revision history

OCA at 10:25, 1 March 2024

OCA — Fri, 01 Mar 2024 10:25:11 GMT

←Older revision		Revision as of 10:25, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3ump' size='340' side='right'caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3ump' size='340' side='right'caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UMP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UMP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ATP:ADENOSINE~~-~~5-TRIPHOSPHATE'>ATP</scene>~~, ~~<scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.849Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3cqd\|3cqd]], [[3umo\|3umo]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">b1723~~, ~~JW5280, PFK2, pfkB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [https://pdbe.org/3ump PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [https://www.ebi.ac.uk/pdbsum/3ump PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ump ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [https://pdbe.org/3ump PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [https://www.ebi.ac.uk/pdbsum/3ump PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ump ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style="background-color:#fffaf0;">~~	+	== Function ==
-	== ~~Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/PFKB_ECOLI PFKB_ECOLI] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.<ref>PMID:16866375</ref>
-	~~The presence of a regulatory site for monovalent cations that affects the conformation of the MgATP-binding pocket leading to enzyme activation has been demonstrated for ribokinases~~. ~~This site is selective toward~~ the ~~ionic radius~~ of ~~the monovalent cation, accepting those larger than Na(+). Phosphofructokinase~~-~~2 (Pfk~~-~~2) from Escherichia coli is homologous~~ to ~~ribokinase~~, but unlike other ribokinase family members, presents an additional site for the nucleotide that negatively regulates its enzymatic activity. In this work, we show the effect of monovalent cations on the kinetic parameters of Pfk-2 together with its three-dimensional structure determined by x-ray diffraction in the presence of K(+) or Cs(+). Kinetic characterization of the enzyme shows that K(+) and Na(+) alter neither the kcat nor the KM values for fructose-6-~~P or MgATP. However, the presence of K(+) (but not Na(+)) enhances the allosteric inhibition induced~~ by ~~MgATP. Moreover~~, ~~binding experiments show that K(+) (but not Na(+)) increases~~ the ~~affinity~~ of ~~MgATP in a saturable fashion~~. In agreement with the biochemical data, the crystal structure of Pfk-2 obtained in the presence of MgATP shows a cation-binding site at the conserved position predicted for the ribokinase family of proteins. This site is adjacent to the MgATP allosteric binding site and is only observed in the presence of Cs(+) or K(+). These results indicate that binding of the monovalent metal ions indirectly influences the allosteric site of Pfk-2 by increasing its affinity for MgATP with no alteration in the conformation of residues present at the catalytic site.	+
-		+
-	A Ribokinase Family Conserved Monovalent Cation Binding Site Enhances the MgATP-induced Inhibition in E. coli Phosphofructokinase-2.,Baez M, Cabrera R, Pereira HM, Blanco A, Villalobos P, Ramirez-Sarmiento CA, Caniuguir A, Guixe V, Garratt RC, Babul J Biophys J. 2013 Jul 2;105(1):185-93. doi: 10.1016/j.bpj.2013.05.028. PMID:23823238<ref>PMID:~~23823238~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ump" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 6-~~phosphofructokinase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Babul, J]]	+	[[Category: Babul J]]
-	[[Category: Baez, M]]	+	[[Category: Baez M]]
-	[[Category: Cabrera, R]]	+	[[Category: Cabrera R]]
-	[[Category: Caniuguir, A]]	+	[[Category: Caniuguir A]]
-	[[Category: Garratt~~, R C~~]]	+	[[Category: Garratt RC]]
-	[[Category: Pereira~~, H M]]~~	+	[[Category: Pereira HM]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Pfk-2]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:48, 20 July 2022

OCA — Wed, 20 Jul 2022 07:48:33 GMT

←Older revision		Revision as of 07:48, 20 July 2022
Line 1:		Line 1:

	==Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP==		==Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP==
-	<StructureSection load='3ump' size='340' side='right' caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3ump' size='340' side='right'caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UMP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UMP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cqd\|3cqd]], [[3umo\|3umo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3cqd\|3cqd]], [[3umo\|3umo]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1723, JW5280, PFK2, pfkB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1723, JW5280, PFK2, pfkB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [~~http~~://pdbe.org/3ump PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ump PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ump ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [https://pdbe.org/3ump PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [https://www.ebi.ac.uk/pdbsum/3ump PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ump ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Phosphofructokinase ~~(PFK)~~\|Phosphofructokinase ~~(PFK)~~]]	+	*[[Phosphofructokinase 3D structures\|Phosphofructokinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Babul, J]]		[[Category: Babul, J]]
	[[Category: Baez, M]]		[[Category: Baez, M]]

OCA at 03:02, 5 August 2016

OCA — Fri, 05 Aug 2016 03:02:31 GMT

←Older revision		Revision as of 03:02, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP==		==Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP==
	<StructureSection load='3ump' size='340' side='right' caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3ump' size='340' side='right' caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UMP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UMP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cqd\|3cqd]], [[3umo\|3umo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cqd\|3cqd]], [[3umo\|3umo]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1723, JW5280, PFK2, pfkB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1723, JW5280, PFK2, pfkB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [http://www.ebi.ac.uk/pdbsum/3ump PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [http://pdbe.org/3ump PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [http://www.ebi.ac.uk/pdbsum/3ump PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ump ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ump" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Babul, J]]		[[Category: Babul, J]]
	[[Category: Baez, M]]		[[Category: Baez, M]]

OCA at 07:00, 21 December 2014

OCA — Sun, 21 Dec 2014 07:00:35 GMT

←Older revision		Revision as of 07:00, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3ump\| PDB=3ump \| SCENE= }}~~	+	==Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP==
-	===Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP===	+	<StructureSection load='3ump' size='340' side='right' caption='[[3ump]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23823238}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UMP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3cqd\|3cqd]], [[3umo\|3umo]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1723, JW5280, PFK2, pfkB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ump FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ump OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ump RCSB], [http://www.ebi.ac.uk/pdbsum/3ump PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The presence of a regulatory site for monovalent cations that affects the conformation of the MgATP-binding pocket leading to enzyme activation has been demonstrated for ribokinases. This site is selective toward the ionic radius of the monovalent cation, accepting those larger than Na(+). Phosphofructokinase-2 (Pfk-2) from Escherichia coli is homologous to ribokinase, but unlike other ribokinase family members, presents an additional site for the nucleotide that negatively regulates its enzymatic activity. In this work, we show the effect of monovalent cations on the kinetic parameters of Pfk-2 together with its three-dimensional structure determined by x-ray diffraction in the presence of K(+) or Cs(+). Kinetic characterization of the enzyme shows that K(+) and Na(+) alter neither the kcat nor the KM values for fructose-6-P or MgATP. However, the presence of K(+) (but not Na(+)) enhances the allosteric inhibition induced by MgATP. Moreover, binding experiments show that K(+) (but not Na(+)) increases the affinity of MgATP in a saturable fashion. In agreement with the biochemical data, the crystal structure of Pfk-2 obtained in the presence of MgATP shows a cation-binding site at the conserved position predicted for the ribokinase family of proteins. This site is adjacent to the MgATP allosteric binding site and is only observed in the presence of Cs(+) or K(+). These results indicate that binding of the monovalent metal ions indirectly influences the allosteric site of Pfk-2 by increasing its affinity for MgATP with no alteration in the conformation of residues present at the catalytic site.

-	~~==About this Structure==~~	+	A Ribokinase Family Conserved Monovalent Cation Binding Site Enhances the MgATP-induced Inhibition in E. coli Phosphofructokinase-2.,Baez M, Cabrera R, Pereira HM, Blanco A, Villalobos P, Ramirez-Sarmiento CA, Caniuguir A, Guixe V, Garratt RC, Babul J Biophys J. 2013 Jul 2;105(1):185-93. doi: 10.1016/j.bpj.2013.05.028. PMID:23823238<ref>PMID:23823238</ref>
-	~~[[3ump]] is a~~ 2 ~~chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k~~-~~12 Escherichia coli k-12]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=3UMP OCA]~~.	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]		*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023823238</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Babul, J.]]	+	[[Category: Babul, J]]
-	[[Category: Baez, M.]]	+	[[Category: Baez, M]]
-	[[Category: Cabrera, R.]]	+	[[Category: Cabrera, R]]
-	[[Category: Caniuguir, A.]]	+	[[Category: Caniuguir, A]]
-	[[Category: Garratt, R C.]]	+	[[Category: Garratt, R C]]
-	[[Category: Pereira, H M.]]	+	[[Category: Pereira, H M]]
	[[Category: Enzyme]]		[[Category: Enzyme]]
	[[Category: Glycolysis]]		[[Category: Glycolysis]]
	[[Category: Pfk-2]]		[[Category: Pfk-2]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 11:40, 24 July 2013

OCA — Wed, 24 Jul 2013 11:40:23 GMT

←Older revision		Revision as of 11:40, 24 July 2013
Line 1:		Line 1:
-	[[Image:3ump.jpg\|left\|200px]]
-
	{{STRUCTURE_3ump\| PDB=3ump \| SCENE= }}		{{STRUCTURE_3ump\| PDB=3ump \| SCENE= }}
-
	===Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP===		===Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP===
-		+	{{ABSTRACT_PUBMED_23823238}}

	==About this Structure==		==About this Structure==
	[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA].		[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA].
		+
		+	==See Also==
		+	*[[Phosphofructokinase (PFK)\|Phosphofructokinase (PFK)]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023823238</ref><references group="xtra"/><references/>
	[[Category: 6-phosphofructokinase]]		[[Category: 6-phosphofructokinase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]

OCA at 06:52, 14 November 2012

OCA — Wed, 14 Nov 2012 06:52:23 GMT

←Older revision		Revision as of 06:52, 14 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ump.jpg\|left\|200px]]

-	~~The entry~~ 3ump ~~is ON HOLD~~ ~~until Nov 13 2013~~	+	{{STRUCTURE_3ump\| PDB=3ump \| SCENE= }}

-	~~Authors: Pereira, H.M., Caniuguir, A., Baez, M., Cabrera, R., Garratt, R.C., Babul, J.~~	+	===Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP===

-	~~Description: Crystal~~ structure ~~of the Phosphofructokinase~~-2 from Escherichia coli ~~in complex with Cesium and ATP~~	+
		+	==About this Structure==
		+	[[3ump]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UMP OCA].
		+	[[Category: 6-phosphofructokinase]]
		+	[[Category: Escherichia coli k-12]]
		+	[[Category: Babul, J.]]
		+	[[Category: Baez, M.]]
		+	[[Category: Cabrera, R.]]
		+	[[Category: Caniuguir, A.]]
		+	[[Category: Garratt, R C.]]
		+	[[Category: Pereira, H M.]]
		+	[[Category: Enzyme]]
		+	[[Category: Glycolysis]]
		+	[[Category: Pfk-2]]
		+	[[Category: Transferase]]

OCA at 06:53, 24 October 2012

OCA — Wed, 24 Oct 2012 06:53:10 GMT

←Older revision		Revision as of 06:53, 24 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ump is ON HOLD until ~~Paper Publication~~	+	The entry 3ump is ON HOLD until Nov 13 2013

	Authors: Pereira, H.M., Caniuguir, A., Baez, M., Cabrera, R., Garratt, R.C., Babul, J.		Authors: Pereira, H.M., Caniuguir, A., Baez, M., Cabrera, R., Garratt, R.C., Babul, J.

	Description: Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP		Description: Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP

OCA at 05:52, 30 November 2011

OCA — Wed, 30 Nov 2011 05:52:57 GMT

←Older revision		Revision as of 05:52, 30 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ump is ON HOLD	+	The entry 3ump is ON HOLD until Paper Publication

-	Authors: Pereira,H.M., Caniuguir, A., Baez, M., Cabrera, R., Babul, J.	+	Authors: Pereira, H.M., Caniuguir, A., Baez, M., Cabrera, R., Garratt, R.C., Babul, J.

-	Description: ~~CRYSTAL STRUCTURE OF THE PHOSPHOFRUCTOKINASE~~-2 ~~FROM ESCHERICHIA COLI IN COMPLEX WITH CESIUM AND~~ ATP	+	Description: Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP

OCA: Protected "3ump" [edit=sysop:move=sysop]

OCA — Wed, 23 Nov 2011 18:39:37 GMT

Protected "3ump" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:39, 23 November 2011

OCA: New page: '''Unreleased structure''' The entry 3ump is ON HOLD Authors: Pereira,H.M., Caniuguir, A., Baez, M., Cabrera, R., Babul, J. Description: CRYSTAL STRUCTURE OF THE PHOSPHOFRUCTOKINASE-2 ...

OCA — Wed, 23 Nov 2011 18:39:36 GMT

New page: '''Unreleased structure''' The entry 3ump is ON HOLD Authors: Pereira,H.M., Caniuguir, A., Baez, M., Cabrera, R., Babul, J. Description: CRYSTAL STRUCTURE OF THE PHOSPHOFRUCTOKINASE-2 ...

New page

'''Unreleased structure'''

The entry 3ump is ON HOLD

Authors: Pereira,H.M., Caniuguir, A., Baez, M., Cabrera, R., Babul, J.

Description: CRYSTAL STRUCTURE OF THE PHOSPHOFRUCTOKINASE-2 FROM ESCHERICHIA COLI IN COMPLEX WITH CESIUM AND ATP