3uoy - Revision history

OCA at 14:04, 14 March 2024

OCA — Thu, 14 Mar 2024 14:04:36 GMT

←Older revision		Revision as of 14:04, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uoy' size='340' side='right'caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3uoy' size='340' side='right'caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UOY FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">otemo ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [https://pdbe.org/3uoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [https://www.ebi.ac.uk/pdbsum/3uoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uoy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [https://pdbe.org/3uoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [https://www.ebi.ac.uk/pdbsum/3uoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uoy ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/OTEMO_PSEPU OTEMO_PSEPU] Involved in the degradation of (+)-camphor. Catalyzes the lactonization of 2-oxo-delta(3)-4,5, 5-trimethylcyclopentenylacetyl-CoA (OT-CoA), a key intermediate in the metabolism of camphor. 2-Oxocyclopentyl ethyl acetate is also a good substrate, as is 2-oxocyclohexyl ethyl acetate and methyl-substituted cyclohexanones, but free acid is a poor substrate.<ref>PMID:22267661</ref> <ref>PMID:22286514</ref> <ref>PMID:6848481</ref>
-	~~A dimeric Baeyer-Villiger monooxygenase~~ (~~BVMO~~) ~~catalyzing~~ the lactonization of 2-oxo-~~Delta~~(3)-4,5,5-trimethylcyclopentenylacetyl-CoA, a key intermediate in the metabolism of camphor ~~by Pseudomonas putida ATCC 17453 had been initially characterized in 1983 by Trudgill and co-workers (H~~.~~J. Ougham, D.G. Taylor, and P.W. Trudgill, J. Bacteriol. 153:140-152, 1983). Here we have cloned and overexpressed the~~ 2-~~oxo-Delta(3)-4~~,~~5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli, and determined its three-dimensional structure with bound FAD at 1.95 A resolution~~ as ~~well as with bound FAD and NADP(+) at~~ 2~~.0 A resolution. OTEMO represents the first homodimeric type 1 BVMO structure bound to FAD/NADP(+). Comparison of several crystal forms of OTEMO bound to FAD~~ and ~~NADP(+) revealed conformational plasticity of several loop regions, some of which have been implicated as contributing to the substrate specificity profile of structurally~~-~~related BVMOs. Substrate specificity studies confirmed that the 2-oxo-Delta(3)-4~~,~~5,5-trimethylcyclopentenylacetic~~ acid ~~coenzyme A ester~~ is ~~preferred over the free acid. However, the catalytic efficiency (k(cat)/K(m)) favors 2-n-hexyl cyclopentanone (4.3 x 10(5) M(-1)s(-1)) as~~ a substrate~~, although its affinity (K(m) = 32 muM) was lower than that of the CoA-activated substrate (18 muM)~~. In whole cell biotransformation experiments, OTEMO showed a unique enantiocomplementarity to the action of the prototypical cyclohexanone monooxygenase (CHMO), and appeared to be particularly useful for the oxidation of 4-substituted cyclohexanones. Overall, this work expands our understanding of the molecular structure and mechanistic complexity of the type 1 family of BVMOs as well as expanding the catalytic repertoire of one of its original members.	+
-		+
-	Camphor Pathway 2-Oxo-{Delta}3-4,5,5-trimethylcyclopentenylacetyl-CoA Monooxygenase of Pseudomonas putida ATCC 17453: Cloning, Baeyer-Villiger Biooxidations, and Structures.,Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC Appl Environ Microbiol. 2012 Jan 20. PMID:22267661<ref>PMID:22267661</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 3uoy" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cygler, M]]	+	[[Category: Pseudomonas putida]]
-	[[Category: Lau, P]]	+	[[Category: Cygler M]]
-	[[Category: Matte, A]]	+	[[Category: Lau P]]
-	[[Category: Shi, R]]	+	[[Category: Matte A]]
-	~~[[Category: Baeyer-villiger monooxygenase]]~~	+	[[Category: Shi R]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:54, 20 July 2022

OCA — Wed, 20 Jul 2022 07:54:18 GMT

←Older revision		Revision as of 07:54, 20 July 2022
Line 1:		Line 1:

	==Crystal Structure of OTEMO complex with FAD and NADP (form 1)==		==Crystal Structure of OTEMO complex with FAD and NADP (form 1)==
-	<StructureSection load='3uoy' size='340' side='right' caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3uoy' size='340' side='right'caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UOY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otemo ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otemo ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [~~http~~://pdbe.org/3uoy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uoy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uoy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [https://pdbe.org/3uoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [https://www.ebi.ac.uk/pdbsum/3uoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uoy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus fluorescens putidus flugge 1886]]		[[Category: Bacillus fluorescens putidus flugge 1886]]
		+	[[Category: Large Structures]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Lau, P]]		[[Category: Lau, P]]

OCA at 13:07, 11 August 2016

OCA — Thu, 11 Aug 2016 13:07:34 GMT

←Older revision		Revision as of 13:07, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of OTEMO complex with FAD and NADP (form 1)==		==Crystal Structure of OTEMO complex with FAD and NADP (form 1)==
	<StructureSection load='3uoy' size='340' side='right' caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3uoy' size='340' side='right' caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_putida Pseudomonas putida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UOY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UOY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otemo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 ~~Pseudomonas putida~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otemo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [http://www.ebi.ac.uk/pdbsum/3uoy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [http://pdbe.org/3uoy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [http://www.ebi.ac.uk/pdbsum/3uoy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uoy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uoy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas putida~~]]	+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Lau, P]]		[[Category: Lau, P]]

OCA at 10:04, 4 January 2015

OCA — Sun, 04 Jan 2015 10:04:45 GMT

←Older revision		Revision as of 10:04, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}~~	+	==Crystal Structure of OTEMO complex with FAD and NADP (form 1)==
-	===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===	+	<StructureSection load='3uoy' size='340' side='right' caption='[[3uoy]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22267661}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uoy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UOY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uov\|3uov]], [[3uox\|3uox]], [[3uoz\|3uoz]], [[3up4\|3up4]], [[3up5\|3up5]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">otemo ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uoy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uoy RCSB], [http://www.ebi.ac.uk/pdbsum/3uoy PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A dimeric Baeyer-Villiger monooxygenase (BVMO) catalyzing the lactonization of 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-CoA, a key intermediate in the metabolism of camphor by Pseudomonas putida ATCC 17453 had been initially characterized in 1983 by Trudgill and co-workers (H.J. Ougham, D.G. Taylor, and P.W. Trudgill, J. Bacteriol. 153:140-152, 1983). Here we have cloned and overexpressed the 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli, and determined its three-dimensional structure with bound FAD at 1.95 A resolution as well as with bound FAD and NADP(+) at 2.0 A resolution. OTEMO represents the first homodimeric type 1 BVMO structure bound to FAD/NADP(+). Comparison of several crystal forms of OTEMO bound to FAD and NADP(+) revealed conformational plasticity of several loop regions, some of which have been implicated as contributing to the substrate specificity profile of structurally-related BVMOs. Substrate specificity studies confirmed that the 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetic acid coenzyme A ester is preferred over the free acid. However, the catalytic efficiency (k(cat)/K(m)) favors 2-n-hexyl cyclopentanone (4.3 x 10(5) M(-1)s(-1)) as a substrate, although its affinity (K(m) = 32 muM) was lower than that of the CoA-activated substrate (18 muM). In whole cell biotransformation experiments, OTEMO showed a unique enantiocomplementarity to the action of the prototypical cyclohexanone monooxygenase (CHMO), and appeared to be particularly useful for the oxidation of 4-substituted cyclohexanones. Overall, this work expands our understanding of the molecular structure and mechanistic complexity of the type 1 family of BVMOs as well as expanding the catalytic repertoire of one of its original members.

-	~~==About this Structure==~~	+	Camphor Pathway 2-Oxo-{Delta}3-4,5,5-trimethylcyclopentenylacetyl-CoA Monooxygenase of Pseudomonas putida ATCC 17453: Cloning, Baeyer-Villiger Biooxidations, and Structures.,Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC Appl Environ Microbiol. 2012 Jan 20. PMID:22267661<ref>PMID:22267661</ref>
-	~~[[3uoy]] is a~~ 2 ~~chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida~~ Pseudomonas putida~~]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3UOY OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:022267661</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
-	[[Category: Cygler, M.]]	+	[[Category: Cygler, M]]
-	[[Category: Lau, P.]]	+	[[Category: Lau, P]]
-	[[Category: Matte, A.]]	+	[[Category: Matte, A]]
-	[[Category: Shi, R.]]	+	[[Category: Shi, R]]
	[[Category: Baeyer-villiger monooxygenase]]		[[Category: Baeyer-villiger monooxygenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 09:38, 19 June 2013

OCA — Wed, 19 Jun 2013 09:38:51 GMT

←Older revision		Revision as of 09:38, 19 June 2013
Line 1:		Line 1:
-	[[Image:3uoy.png\|left\|200px]]
-
	{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}		{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}
-
	===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===		===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===
-
	{{ABSTRACT_PUBMED_22267661}}		{{ABSTRACT_PUBMED_22267661}}

Line 11:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:022267661</ref><references group="xtra"/>	+	<ref group="xtra">PMID:022267661</ref><references group="xtra"/><references/>
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]

OCA at 10:18, 8 August 2012

OCA — Wed, 08 Aug 2012 10:18:52 GMT

←Older revision		Revision as of 10:18, 8 August 2012
Line 1:		Line 1:
-	[[Image:3uoy.~~jpg~~\|left\|200px]]	+	[[Image:3uoy.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uoy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}		{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}

	===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===		===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22267661}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22267661 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22267661}}		{{ABSTRACT_PUBMED_22267661}}

OCA at 07:18, 1 February 2012

OCA — Wed, 01 Feb 2012 07:18:47 GMT

←Older revision		Revision as of 07:18, 1 February 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3uoy.jpg\|left\|200px]]

-	The ~~entry 3uoy~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uoy", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uoy\| PDB=3uoy \| SCENE= }}

-	~~Authors: Shi, R., Matte, A., Cygler, M., Lau, P.~~	+	===Crystal Structure of OTEMO complex with FAD and NADP (form 1)===

-	~~Description~~: ~~Crystal~~ Structure ~~of OTEMO complex~~ with ~~FAD and NADP (form 1)~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22267661}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22267661 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22267661}}
		+
		+	==About this Structure==
		+	[[3uoy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOY OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022267661</ref><references group="xtra"/>
		+	[[Category: Pseudomonas putida]]
		+	[[Category: Cygler, M.]]
		+	[[Category: Lau, P.]]
		+	[[Category: Matte, A.]]
		+	[[Category: Shi, R.]]
		+	[[Category: Baeyer-villiger monooxygenase]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "3uoy" [edit=sysop:move=sysop]

OCA — Wed, 23 Nov 2011 18:42:04 GMT

Protected "3uoy" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:42, 23 November 2011

OCA: New page: '''Unreleased structure''' The entry 3uoy is ON HOLD Authors: Shi, R., Matte, A., Cygler, M., Lau, P. Description: Crystal Structure of OTEMO complex with FAD and NADP (form 1)

OCA — Wed, 23 Nov 2011 18:42:03 GMT

New page: '''Unreleased structure''' The entry 3uoy is ON HOLD Authors: Shi, R., Matte, A., Cygler, M., Lau, P. Description: Crystal Structure of OTEMO complex with FAD and NADP (form 1)

New page

'''Unreleased structure'''

The entry 3uoy is ON HOLD

Authors: Shi, R., Matte, A., Cygler, M., Lau, P.

Description: Crystal Structure of OTEMO complex with FAD and NADP (form 1)