3upk - Revision history

OCA at 14:05, 14 March 2024

OCA — Thu, 14 Mar 2024 14:05:07 GMT

←Older revision		Revision as of 14:05, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3upk' size='340' side='right'caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3upk' size='340' side='right'caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter~~ cloacae~~" (jordan 1890) bergey et al. 1923~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UPK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UPK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">ECL_04571~~, ~~murA, murZ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP~~-~~N-acetylglucosamine_1-carboxyvinyltransferase UDP~~-N-~~acetylglucosamine 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [https://pdbe.org/3upk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [https://www.ebi.ac.uk/pdbsum/3upk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3upk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [https://pdbe.org/3upk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [https://www.ebi.ac.uk/pdbsum/3upk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3upk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MURA_ENTCC MURA_ENTCC]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic phosphomycin.	+	[https://www.uniprot.org/uniprot/MURA_ENTCC MURA_ENTCC] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic phosphomycin.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The enzyme MurA has been an established antibiotic target since the discovery of fosfomycin, which specifically inhibits MurA by covalent modification of the active site residue Cys115. Early biochemical studies established that Cys115 also covalently reacts with substrate phosphoenolpyruvate (PEP) to yield a phospholactoyl adduct, but the structural and functional consequences of this reaction remained obscure. We captured and depicted the Cys115-PEP adduct of E. cloacace MurA in various reaction states by X-ray crystallography. The data suggest that cellular MurA predominantly exists in a tightly locked complex with UDP-N-acetylmuramic acid (UNAM), the product of the MurB reaction, with PEP covalently attached to Cys115. The uniqueness and rigidity of this dormant complex was previously not recognized and presumably accounts for the failure of drug discovery efforts towards the identification of novel and effective MurA inhibitors. We demonstrate that recently published crystal structures of MurA from various organisms determined by different laboratories were indeed misinterpreted and actually contain UNAM and covalently bound PEP. The Cys115-PEP adduct was also captured in vitro during the reaction of free MurA and substrate UDP-N-acetylglucosamine (UNAG) or isomer UDP-N-acetylgalactosamine. The now available series of crystal structures allows a comprehensive view of the reaction cycle of MurA. It appears that the covalent reaction of MurA with PEP fulfills dual functions by tightening the complex with UNAM for the efficient feedback regulation of murein biosynthesis and by priming the PEP molecule for instantaneous reaction with substrate UNAG.	+
-		+
-	Functional consequence of the covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).,Zhu JY, Yang Y, Han H, Betzi S, Olesen S, Marsilio F, Schonbrunn E J Biol Chem. 2012 Feb 29. PMID:22378791<ref>PMID:22378791</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3upk" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]		*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterobacter cloacae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]~~	+	[[Category: Schonbrunn E]]
-	[[Category: Schonbrunn, E]]	+	[[Category: Yang Y]]
-	[[Category: Yang, Y]]	+	[[Category: Zhu J-Y]]
-	[[Category: Zhu, J Y]]	+
-	~~[[Category: Biogenesis/degradation]]~~	+
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: Close enzyme state]]~~	+
-	~~[[Category: Mura]]~~	+
-	~~[[Category: Peptidoglycan synthesis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:55, 20 July 2022

OCA — Wed, 20 Jul 2022 07:55:45 GMT

←Older revision		Revision as of 07:55, 20 July 2022
Line 1:		Line 1:

	==E. cloacae MURA in complex with UNAG==		==E. cloacae MURA in complex with UNAG==
-	<StructureSection load='3upk' size='340' side='right' caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3upk' size='340' side='right'caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UPK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UPK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECL_04571, murA, murZ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECL_04571, murA, murZ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [~~http~~://pdbe.org/3upk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3upk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3upk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [https://pdbe.org/3upk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [https://www.ebi.ac.uk/pdbsum/3upk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3upk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MURA_ENTCC MURA_ENTCC]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic phosphomycin.	+	[[https://www.uniprot.org/uniprot/MURA_ENTCC MURA_ENTCC]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic phosphomycin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Enoylpyruvate transferase\|Enoylpyruvate transferase]]	+	*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
	[[Category: Schonbrunn, E]]		[[Category: Schonbrunn, E]]

OCA at 20:33, 4 August 2016

OCA — Thu, 04 Aug 2016 20:33:21 GMT

←Older revision		Revision as of 20:33, 4 August 2016
Line 1:		Line 1:
		+
	==E. cloacae MURA in complex with UNAG==		==E. cloacae MURA in complex with UNAG==
	<StructureSection load='3upk' size='340' side='right' caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3upk' size='340' side='right' caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacter_cloacae Enterobacter~~ cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UPK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UPK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECL_04571, murA, murZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 ~~Enterobacter~~ cloacae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECL_04571, murA, murZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [http://www.ebi.ac.uk/pdbsum/3upk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [http://pdbe.org/3upk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [http://www.ebi.ac.uk/pdbsum/3upk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3upk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3upk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterobacter cloacae]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
	[[Category: Schonbrunn, E]]		[[Category: Schonbrunn, E]]

OCA at 19:22, 24 December 2014

OCA — Wed, 24 Dec 2014 19:22:53 GMT

←Older revision		Revision as of 19:22, 24 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3upk.~~png~~\|~~left~~\|~~200px~~]]	+	==E. cloacae MURA in complex with UNAG==
		+	<StructureSection load='3upk' size='340' side='right' caption='[[3upk]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UPK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECL_04571, murA, murZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 Enterobacter cloacae])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3upk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3upk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3upk RCSB], [http://www.ebi.ac.uk/pdbsum/3upk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MURA_ENTCC MURA_ENTCC]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic phosphomycin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzyme MurA has been an established antibiotic target since the discovery of fosfomycin, which specifically inhibits MurA by covalent modification of the active site residue Cys115. Early biochemical studies established that Cys115 also covalently reacts with substrate phosphoenolpyruvate (PEP) to yield a phospholactoyl adduct, but the structural and functional consequences of this reaction remained obscure. We captured and depicted the Cys115-PEP adduct of E. cloacace MurA in various reaction states by X-ray crystallography. The data suggest that cellular MurA predominantly exists in a tightly locked complex with UDP-N-acetylmuramic acid (UNAM), the product of the MurB reaction, with PEP covalently attached to Cys115. The uniqueness and rigidity of this dormant complex was previously not recognized and presumably accounts for the failure of drug discovery efforts towards the identification of novel and effective MurA inhibitors. We demonstrate that recently published crystal structures of MurA from various organisms determined by different laboratories were indeed misinterpreted and actually contain UNAM and covalently bound PEP. The Cys115-PEP adduct was also captured in vitro during the reaction of free MurA and substrate UDP-N-acetylglucosamine (UNAG) or isomer UDP-N-acetylgalactosamine. The now available series of crystal structures allows a comprehensive view of the reaction cycle of MurA. It appears that the covalent reaction of MurA with PEP fulfills dual functions by tightening the complex with UNAM for the efficient feedback regulation of murein biosynthesis and by priming the PEP molecule for instantaneous reaction with substrate UNAG.

-	<!--	+	Functional consequence of the covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).,Zhu JY, Yang Y, Han H, Betzi S, Olesen S, Marsilio F, Schonbrunn E J Biol Chem. 2012 Feb 29. PMID:22378791<ref>PMID:22378791</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3upk", creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3upk\| PDB=3upk \| SCENE= }}~~	+

-	~~===E~~. ~~cloacae MURA in complex with UNAG===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Enoylpyruvate transferase\|Enoylpyruvate transferase]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22378791}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22378791 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22378791}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3upk~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022378791<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacter cloacae]]		[[Category: Enterobacter cloacae]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
-	[[Category: Schonbrunn, E.]]	+	[[Category: Schonbrunn, E]]
-	[[Category: Yang, Y.]]	+	[[Category: Yang, Y]]
-	[[Category: Zhu, J Y.]]	+	[[Category: Zhu, J Y]]
	[[Category: Biogenesis/degradation]]		[[Category: Biogenesis/degradation]]
	[[Category: Cell wall]]		[[Category: Cell wall]]

OCA at 06:55, 21 March 2012

OCA — Wed, 21 Mar 2012 06:55:56 GMT

←Older revision		Revision as of 06:55, 21 March 2012
Line 1:		Line 1:
-	[[Image:3upk.~~jpg~~\|left\|200px]]	+	[[Image:3upk.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===E. cloacae MURA in complex with UNAG===		===E. cloacae MURA in complex with UNAG===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22378791}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22378791 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22378791}}

	==About this Structure==		==About this Structure==
	[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA].		[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022378791</ref><references group="xtra"/>
	[[Category: Enterobacter cloacae]]		[[Category: Enterobacter cloacae]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]

OCA at 05:56, 8 March 2012

OCA — Thu, 08 Mar 2012 05:56:08 GMT

←Older revision		Revision as of 05:56, 8 March 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3upk.jpg\|left\|200px]]

-	The ~~entry 3upk~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3upk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3upk\| PDB=3upk \| SCENE= }}

-	~~Authors: Zhu, J.-Y., Yang, Y., Schonbrunn,~~ E.	+	===E. cloacae MURA in complex with UNAG===

-	~~Description~~: E. cloacae ~~MURA in complex with UNAG~~	+
		+	==About this Structure==
		+	[[3upk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UPK OCA].
		+	[[Category: Enterobacter cloacae]]
		+	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
		+	[[Category: Schonbrunn, E.]]
		+	[[Category: Yang, Y.]]
		+	[[Category: Zhu, J Y.]]
		+	[[Category: Biogenesis/degradation]]
		+	[[Category: Cell wall]]
		+	[[Category: Close enzyme state]]
		+	[[Category: Mura]]
		+	[[Category: Peptidoglycan synthesis]]
		+	[[Category: Transferase]]

OCA at 06:00, 8 February 2012

OCA — Wed, 08 Feb 2012 06:00:31 GMT

←Older revision		Revision as of 06:00, 8 February 2012
Line 5:		Line 5:
	Authors: Zhu, J.-Y., Yang, Y., Schonbrunn, E.		Authors: Zhu, J.-Y., Yang, Y., Schonbrunn, E.

-	Description: MURA in complex with ~~UD1~~	+	Description: E. cloacae MURA in complex with UNAG

OCA: Protected "3upk" [edit=sysop:move=sysop]

OCA — Wed, 30 Nov 2011 05:53:40 GMT

Protected "3upk" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:53, 30 November 2011

OCA: New page: '''Unreleased structure''' The entry 3upk is ON HOLD until Paper Publication Authors: Zhu, J.-Y., Yang, Y., Schonbrunn, E. Description: MURA in complex with UD1

OCA — Wed, 30 Nov 2011 05:53:39 GMT

New page: '''Unreleased structure''' The entry 3upk is ON HOLD until Paper Publication Authors: Zhu, J.-Y., Yang, Y., Schonbrunn, E. Description: MURA in complex with UD1

New page

'''Unreleased structure'''

The entry 3upk is ON HOLD until Paper Publication

Authors: Zhu, J.-Y., Yang, Y., Schonbrunn, E.

Description: MURA in complex with UD1