3uq5 - Revision history

OCA at 14:05, 14 March 2024

OCA — Thu, 14 Mar 2024 14:05:58 GMT

←Older revision		Revision as of 14:05, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uq5' size='340' side='right'caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='3uq5' size='340' side='right'caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dicd3 Dicd3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3uq4\|3uq4]], [[3uq7\|3uq7]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Dda3937_00520 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [https://pdbe.org/3uq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [https://pdbe.org/3uq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq5 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
-	The determination of structural models of the various stable states of an ion channel is a key step toward the characterization of its conformational dynamics. In the case of nicotinic-type receptors, different structures have been solved but, thus far, these different models have been obtained from different members of the superfamily. In the case of the bacterial member ELIC, a cysteamine-gated channel from Erwinia chrisanthemi, a structural model of the protein in the absence of activating ligand (and thus, conceivably corresponding to the closed state of this channel) has been previously generated. In this article, electrophysiological characterization of ELIC mutants allowed us to identify pore mutations that slow down the time course of desensitization to the extent that the channel seems not to desensitize at all for the duration of the agonist applications (>20 min). Thus, it seems reasonable to conclude that the probability of ELIC occupying the closed state is much lower for the ligand-bound mutants than for the unliganded wild-type channel. To gain insight into the conformation adopted by ELIC under these conditions, we solved the crystal structures of two of these mutants in the presence of a concentration of cysteamine that elicits an intracluster open probability of >0.9. Curiously, the obtained structural models turned out to be nearly indistinguishable from the model of the wild-type channel in the absence of bound agonist. Overall, our findings bring to light the limited power of functional studies in intact membranes when it comes to inferring the functional state of a channel in a crystal, at least in the case of the nicotinic-receptor superfamily.	+

-	Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>	+	==See Also==
-		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3uq5" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicd3~~]]	+	[[Category: Dickeya dadantii 3937]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agarwal, V]]	+	[[Category: Agarwal V]]
-	[[Category: Gonzalez-Gutierrez, G]]	+	[[Category: Gonzalez-Gutierrez G]]
-	[[Category: Grosman, C]]	+	[[Category: Grosman C]]
-	[[Category: Lukk, T]]	+	[[Category: Lukk T]]
-	[[Category: Nair~~, S K~~]]	+	[[Category: Nair SK]]
-	[[Category: Papke, D]]	+	[[Category: Papke D]]
-	~~[[Category: Liganded-gated ion channel]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:57, 20 July 2022

OCA — Wed, 20 Jul 2022 07:57:22 GMT

←Older revision		Revision as of 07:57, 20 July 2022
Line 3:		Line 3:
	<StructureSection load='3uq5' size='340' side='right'caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='3uq5' size='340' side='right'caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uq4\|3uq4]], [[3uq7\|3uq7]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [~~http~~://pdbe.org/3uq5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uq5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [https://pdbe.org/3uq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [https://www.ebi.ac.uk/pdbsum/3uq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:43, 17 July 2019

OCA — Wed, 17 Jul 2019 11:43:52 GMT

←Older revision		Revision as of 11:43, 17 July 2019
Line 1:		Line 1:

	==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine==		==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine==
-	<StructureSection load='3uq5' size='340' side='right' caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>	+	<StructureSection load='3uq5' size='340' side='right'caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Dicd3]]		[[Category: Dicd3]]
		+	[[Category: Large Structures]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Gonzalez-Gutierrez, G]]		[[Category: Gonzalez-Gutierrez, G]]

OCA at 20:03, 11 August 2016

OCA — Thu, 11 Aug 2016 20:03:40 GMT

←Older revision		Revision as of 20:03, 11 August 2016
Line 1:		Line 1:
		+
	==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine==		==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine==
	<StructureSection load='3uq5' size='340' side='right' caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='3uq5' size='340' side='right' caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Erwinia_chrysanthemi Erwinia chrysanthemi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~556 Erwinia chrysanthemi~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [http://pdbe.org/3uq5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uq5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uq5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Erwinia chrysanthemi~~]]	+	[[Category: Dicd3]]
	[[Category: Agarwal, V]]		[[Category: Agarwal, V]]
	[[Category: Gonzalez-Gutierrez, G]]		[[Category: Gonzalez-Gutierrez, G]]

OCA at 09:45, 4 January 2015

OCA — Sun, 04 Jan 2015 09:45:31 GMT

←Older revision		Revision as of 09:45, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq5 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>		Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Erwinia chrysanthemi]]		[[Category: Erwinia chrysanthemi]]
-	[[Category: Agarwal, V.]]	+	[[Category: Agarwal, V]]
-	[[Category: Gonzalez-Gutierrez, G.]]	+	[[Category: Gonzalez-Gutierrez, G]]
-	[[Category: Grosman, C.]]	+	[[Category: Grosman, C]]
-	[[Category: Lukk, T.]]	+	[[Category: Lukk, T]]
-	[[Category: Nair, S K.]]	+	[[Category: Nair, S K]]
-	[[Category: Papke, D.]]	+	[[Category: Papke, D]]
	[[Category: Liganded-gated ion channel]]		[[Category: Liganded-gated ion channel]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 06:20, 5 June 2014

OCA — Thu, 05 Jun 2014 06:20:26 GMT

←Older revision		Revision as of 06:20, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3uq5.~~png~~\|~~left~~\|~~200px~~]]	+	==X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine==
		+	<StructureSection load='3uq5' size='340' side='right' caption='[[3uq5]], [[Resolution\|resolution]] 4.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UQ5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uq4\|3uq4]], [[3uq7\|3uq7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uq5 RCSB], [http://www.ebi.ac.uk/pdbsum/3uq5 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The determination of structural models of the various stable states of an ion channel is a key step toward the characterization of its conformational dynamics. In the case of nicotinic-type receptors, different structures have been solved but, thus far, these different models have been obtained from different members of the superfamily. In the case of the bacterial member ELIC, a cysteamine-gated channel from Erwinia chrisanthemi, a structural model of the protein in the absence of activating ligand (and thus, conceivably corresponding to the closed state of this channel) has been previously generated. In this article, electrophysiological characterization of ELIC mutants allowed us to identify pore mutations that slow down the time course of desensitization to the extent that the channel seems not to desensitize at all for the duration of the agonist applications (>20 min). Thus, it seems reasonable to conclude that the probability of ELIC occupying the closed state is much lower for the ligand-bound mutants than for the unliganded wild-type channel. To gain insight into the conformation adopted by ELIC under these conditions, we solved the crystal structures of two of these mutants in the presence of a concentration of cysteamine that elicits an intracluster open probability of >0.9. Curiously, the obtained structural models turned out to be nearly indistinguishable from the model of the wild-type channel in the absence of bound agonist. Overall, our findings bring to light the limited power of functional studies in intact membranes when it comes to inferring the functional state of a channel in a crystal, at least in the case of the nicotinic-receptor superfamily.

-	~~<!--~~	+	Mutations that stabilize the open state of the Erwinia chrisanthemi ligand-gated ion channel fail to change the conformation of the pore domain in crystals.,Gonzalez-Gutierrez G, Lukk T, Agarwal V, Papke D, Nair SK, Grosman C Proc Natl Acad Sci U S A. 2012 Apr 2. PMID:22474383<ref>PMID:22474383</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3uq5", creates~~ the ~~"Structure Box" on~~ the ~~page.~~	+
-	~~You may~~ change the ~~PDB parameter (which sets~~ the ~~PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3uq5\| PDB=3uq5 \| SCENE= }}~~	+

-	~~===X-ray structure of~~ a ~~pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence~~ of ~~10 mM cysteamine===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22474383}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22474383 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22474383}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3uq5]] is a 10 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022474383<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Erwinia chrysanthemi]]		[[Category: Erwinia chrysanthemi]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 06:53, 18 April 2012

OCA — Wed, 18 Apr 2012 06:53:00 GMT

←Older revision		Revision as of 06:53, 18 April 2012
Line 1:		Line 1:
-	[[Image:3uq5.~~jpg~~\|left\|200px]]	+	[[Image:3uq5.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine===		===X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22474383}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22474383 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22474383}}

	==About this Structure==		==About this Structure==
	[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA].		[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022474383</ref><references group="xtra"/>
	[[Category: Erwinia chrysanthemi]]		[[Category: Erwinia chrysanthemi]]
	[[Category: Agarwal, V.]]		[[Category: Agarwal, V.]]

OCA at 06:17, 4 April 2012

OCA — Wed, 04 Apr 2012 06:17:16 GMT

←Older revision		Revision as of 06:17, 4 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uq5.jpg\|left\|200px]]

-	The ~~entry 3uq5~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uq5", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uq5\| PDB=3uq5 \| SCENE= }}

-	~~Authors: Gonzalez~~-~~Gutierrez, G., Lukk, T., Agarwal, V., Papke, D., Nair, S.K., Grosman, C.~~	+	===X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine===

-	~~Description~~:	+
		+	==About this Structure==
		+	[[3uq5]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UQ5 OCA].
		+	[[Category: Erwinia chrysanthemi]]
		+	[[Category: Agarwal, V.]]
		+	[[Category: Gonzalez-Gutierrez, G.]]
		+	[[Category: Grosman, C.]]
		+	[[Category: Lukk, T.]]
		+	[[Category: Nair, S K.]]
		+	[[Category: Papke, D.]]
		+	[[Category: Liganded-gated ion channel]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transport protein]]

OCA at 06:41, 28 December 2011

OCA — Wed, 28 Dec 2011 06:41:36 GMT

←Older revision		Revision as of 06:41, 28 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uq5 is ON HOLD	+	The entry 3uq5 is ON HOLD until Paper Publication

	Authors: Gonzalez-Gutierrez, G., Lukk, T., Agarwal, V., Papke, D., Nair, S.K., Grosman, C.		Authors: Gonzalez-Gutierrez, G., Lukk, T., Agarwal, V., Papke, D., Nair, S.K., Grosman, C.

	Description:		Description:

OCA at 07:37, 7 December 2011

OCA — Wed, 07 Dec 2011 07:37:02 GMT

←Older revision		Revision as of 07:37, 7 December 2011
Line 5:		Line 5:
	Authors: Gonzalez-Gutierrez, G., Lukk, T., Agarwal, V., Papke, D., Nair, S.K., Grosman, C.		Authors: Gonzalez-Gutierrez, G., Lukk, T., Agarwal, V., Papke, D., Nair, S.K., Grosman, C.

-	Description: ~~Protein 1~~	+	Description: