3utc - Revision history

OCA at 14:07, 14 March 2024

OCA — Thu, 14 Mar 2024 14:07:58 GMT

←Older revision		Revision as of 14:07, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3utc' size='340' side='right'caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3utc' size='340' side='right'caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UTC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UTC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=0JX:(3E)~~-~~4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>~~, ~~<scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ispH, lytB, yaaE, b0029, JW0027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=83333 ECOLI])</td></tr>~~	+
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2~~-~~enyl_diphosphate_reductase~~ 4-~~hydroxy~~-3-~~methylbut~~-2-~~enyl diphosphate reductase]~~, ~~with EC number [https~~:/~~/www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [https://pdbe.org/3utc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [https://www.ebi.ac.uk/pdbsum/3utc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3utc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [https://pdbe.org/3utc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [https://www.ebi.ac.uk/pdbsum/3utc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3utc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>	+	[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The final step of the methylerythritol phosphate isoprenoid biosynthesis pathway is catalysed by the iron-sulphur enzyme IspH, producing the universal precursors of terpenes: isopentenyl diphosphate and dimethylallyl diphosphate. Here we report an unforeseen reaction discovered during the investigation of the interaction of IspH with acetylene inhibitors by X-ray crystallography, Mossbauer, and nuclear magnetic resonance spectroscopy. In addition to its role as a 2H(+)/2e(-) reductase, IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. The reactions only occur with the oxidised protein and proceed via eta(1)-O-enolate intermediates. One of these is characterized crystallographically and contains a C4 ligand oxygen bound to the unique, fourth iron in the 4Fe-4S cluster: this intermediate subsequently hydrolyzes to produce an aldehyde product. This unexpected side to IspH reactivity is of interest in the context of the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives targeting IspH.	+
-		+
-	Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.,Span I, Wang K, Wang W, Zhang Y, Bacher A, Eisenreich W, Li K, Schulz C, Oldfield E, Groll M Nat Commun. 2012 Sep 4;3:1042. doi: 10.1038/ncomms2052. PMID:22948824<ref>PMID:22948824</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3utc" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 4-~~hydroxy-3-methylbut-2-enyl diphosphate reductase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bacher, A]]	+	[[Category: Bacher A]]
-	[[Category: Eisenreich, W]]	+	[[Category: Eisenreich W]]
-	[[Category: Groll, M]]	+	[[Category: Groll M]]
-	[[Category: Li, K]]	+	[[Category: Li K]]
-	[[Category: Oldfield, E]]	+	[[Category: Oldfield E]]
-	[[Category: Schulz, C]]	+	[[Category: Schulz C]]
-	[[Category: Span, I]]	+	[[Category: Span I]]
-	[[Category: Wang, K]]	+	[[Category: Wang K]]
-	[[Category: Wang, W]]	+	[[Category: Wang W]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang Y]]
-	~~[[Category: Iron-sulfur protein]]~~	+
-	~~[[Category: Isoprenoid biosynthesis]]~~	+
-	~~[[Category: Isph]]~~	+
-	~~[[Category: Lytb]]~~	+
-	~~[[Category: Non-mevalonate pathway]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:04, 20 July 2022

OCA — Wed, 20 Jul 2022 08:04:59 GMT

←Older revision		Revision as of 08:04, 20 July 2022
Line 1:		Line 1:

	==Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate==		==Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate==
-	<StructureSection load='3utc' size='340' side='right' caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3utc' size='340' side='right'caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UTC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UTC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [~~http~~://pdbe.org/3utc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3utc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3utc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [https://pdbe.org/3utc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [https://www.ebi.ac.uk/pdbsum/3utc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3utc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>	+	[[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]	+	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Bacher, A]]		[[Category: Bacher, A]]
	[[Category: Eisenreich, W]]		[[Category: Eisenreich, W]]

OCA at 19:56, 4 August 2016

OCA — Thu, 04 Aug 2016 19:56:03 GMT

←Older revision		Revision as of 19:56, 4 August 2016
Line 1:		Line 1:
		+
	==Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate==		==Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate==
	<StructureSection load='3utc' size='340' side='right' caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3utc' size='340' side='right' caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UTC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UTC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [http://www.ebi.ac.uk/pdbsum/3utc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [http://pdbe.org/3utc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [http://www.ebi.ac.uk/pdbsum/3utc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3utc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3utc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Bacher, A]]		[[Category: Bacher, A]]
	[[Category: Eisenreich, W]]		[[Category: Eisenreich, W]]

OCA at 12:37, 25 December 2014

OCA — Thu, 25 Dec 2014 12:37:46 GMT

←Older revision		Revision as of 12:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UTC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UTC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [http://www.ebi.ac.uk/pdbsum/3utc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [http://www.ebi.ac.uk/pdbsum/3utc PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Bacher, A.]]	+	[[Category: Bacher, A]]
-	[[Category: Eisenreich, W.]]	+	[[Category: Eisenreich, W]]
-	[[Category: Groll, M.]]	+	[[Category: Groll, M]]
-	[[Category: Li, K.]]	+	[[Category: Li, K]]
-	[[Category: Oldfield, E.]]	+	[[Category: Oldfield, E]]
-	[[Category: Schulz, C.]]	+	[[Category: Schulz, C]]
-	[[Category: Span, I.]]	+	[[Category: Span, I]]
-	[[Category: Wang, K.]]	+	[[Category: Wang, K]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Iron-sulfur protein]]		[[Category: Iron-sulfur protein]]
	[[Category: Isoprenoid biosynthesis]]		[[Category: Isoprenoid biosynthesis]]

OCA at 06:52, 25 June 2014

OCA — Wed, 25 Jun 2014 06:52:32 GMT

←Older revision		Revision as of 06:52, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3utc.~~png~~\|~~left~~\|~~200px~~]]	+	==Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate==
		+	<StructureSection load='3utc' size='340' side='right' caption='[[3utc]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UTC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]], [[3urk\|3urk]], [[3utd\|3utd]], [[3uv3\|3uv3]], [[3uv6\|3uv6]], [[3uv7\|3uv7]], [[3uv8\|3uv8]], [[3uwm\|3uwm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3utc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3utc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3utc RCSB], [http://www.ebi.ac.uk/pdbsum/3utc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The final step of the methylerythritol phosphate isoprenoid biosynthesis pathway is catalysed by the iron-sulphur enzyme IspH, producing the universal precursors of terpenes: isopentenyl diphosphate and dimethylallyl diphosphate. Here we report an unforeseen reaction discovered during the investigation of the interaction of IspH with acetylene inhibitors by X-ray crystallography, Mossbauer, and nuclear magnetic resonance spectroscopy. In addition to its role as a 2H(+)/2e(-) reductase, IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. The reactions only occur with the oxidised protein and proceed via eta(1)-O-enolate intermediates. One of these is characterized crystallographically and contains a C4 ligand oxygen bound to the unique, fourth iron in the 4Fe-4S cluster: this intermediate subsequently hydrolyzes to produce an aldehyde product. This unexpected side to IspH reactivity is of interest in the context of the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives targeting IspH.

-	~~{{STRUCTURE_3utc\| PDB=3utc \| SCENE= }}~~	+	Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.,Span I, Wang K, Wang W, Zhang Y, Bacher A, Eisenreich W, Li K, Schulz C, Oldfield E, Groll M Nat Commun. 2012 Sep 4;3:1042. doi: 10.1038/ncomms2052. PMID:22948824<ref>PMID:22948824</ref>

-	~~===Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22948824}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3utc]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA].~~	+	</StructureSection>
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 06:27, 19 September 2012

OCA — Wed, 19 Sep 2012 06:27:01 GMT

←Older revision		Revision as of 06:27, 19 September 2012
Line 5:		Line 5:
	===Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate===		===Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate===

		+	{{ABSTRACT_PUBMED_22948824}}

	==About this Structure==		==About this Structure==

OCA at 05:26, 12 September 2012

OCA — Wed, 12 Sep 2012 05:26:51 GMT

←Older revision		Revision as of 05:26, 12 September 2012
Line 1:		Line 1:
-	[[Image:3utc.~~jpg~~\|left\|200px]]	+	[[Image:3utc.png\|left\|200px]]

	{{STRUCTURE_3utc\| PDB=3utc \| SCENE= }}		{{STRUCTURE_3utc\| PDB=3utc \| SCENE= }}

-	===Ec_IspH in complex with (Z)-4-hydroxybut-3-enyl diphosphate===	+	===Ec_IspH in complex with (E)-4-hydroxybut-3-enyl diphosphate===


Line 13:		Line 13:
	[[Category: Eisenreich, W.]]		[[Category: Eisenreich, W.]]
	[[Category: Groll, M.]]		[[Category: Groll, M.]]
		+	[[Category: Li, K.]]
	[[Category: Oldfield, E.]]		[[Category: Oldfield, E.]]
	[[Category: Schulz, C.]]		[[Category: Schulz, C.]]

OCA at 05:31, 5 September 2012

OCA — Wed, 05 Sep 2012 05:31:38 GMT

←Older revision		Revision as of 05:31, 5 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3utc.jpg\|left\|200px]]

-	~~The entry~~ 3utc ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3utc\| PDB=3utc \| SCENE= }}

-	~~Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.~~	+	===Ec_IspH in complex with (Z)-4-hydroxybut-3-enyl diphosphate===

-	~~Description: Ec_IspH in complex~~ with ~~(Z)~~-4-~~hydroxybut~~-3-enyl diphosphate	+
		+	==About this Structure==
		+	[[3utc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UTC OCA].
		+	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Bacher, A.]]
		+	[[Category: Eisenreich, W.]]
		+	[[Category: Groll, M.]]
		+	[[Category: Oldfield, E.]]
		+	[[Category: Schulz, C.]]
		+	[[Category: Span, I.]]
		+	[[Category: Wang, K.]]
		+	[[Category: Wang, W.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Iron-sulfur protein]]
		+	[[Category: Isoprenoid biosynthesis]]
		+	[[Category: Isph]]
		+	[[Category: Lytb]]
		+	[[Category: Non-mevalonate pathway]]
		+	[[Category: Oxidoreductase]]

OCA at 08:13, 29 February 2012

OCA — Wed, 29 Feb 2012 08:13:54 GMT

←Older revision		Revision as of 08:13, 29 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3utc is ON HOLD	+	The entry 3utc is ON HOLD until Paper Publication

	Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.		Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.

	Description: Ec_IspH in complex with (Z)-4-hydroxybut-3-enyl diphosphate		Description: Ec_IspH in complex with (Z)-4-hydroxybut-3-enyl diphosphate

OCA: Protected "3utc" [edit=sysop:move=sysop]

OCA — Wed, 07 Dec 2011 07:38:09 GMT

Protected "3utc" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:38, 7 December 2011