3uwm - Revision history

OCA at 14:10, 14 March 2024

OCA — Thu, 14 Mar 2024 14:10:36 GMT

←Older revision		Revision as of 14:10, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uwm' size='340' side='right'caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3uwm' size='340' side='right'caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ke8\|3ke8]], [[3dnf\|3dnf]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [https://pdbe.org/3uwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [https://www.ebi.ac.uk/pdbsum/3uwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [https://pdbe.org/3uwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [https://www.ebi.ac.uk/pdbsum/3uwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>	+	[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The final step of the methylerythritol phosphate isoprenoid biosynthesis pathway is catalysed by the iron-sulphur enzyme IspH, producing the universal precursors of terpenes: isopentenyl diphosphate and dimethylallyl diphosphate. Here we report an unforeseen reaction discovered during the investigation of the interaction of IspH with acetylene inhibitors by X-ray crystallography, Mossbauer, and nuclear magnetic resonance spectroscopy. In addition to its role as a 2H(+)/2e(-) reductase, IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. The reactions only occur with the oxidised protein and proceed via eta(1)-O-enolate intermediates. One of these is characterized crystallographically and contains a C4 ligand oxygen bound to the unique, fourth iron in the 4Fe-4S cluster: this intermediate subsequently hydrolyzes to produce an aldehyde product. This unexpected side to IspH reactivity is of interest in the context of the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives targeting IspH.	+
-		+
-	Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.,Span I, Wang K, Wang W, Zhang Y, Bacher A, Eisenreich W, Li K, Schulz C, Oldfield E, Groll M Nat Commun. 2012 Sep 4;3:1042. doi: 10.1038/ncomms2052. PMID:22948824<ref>PMID:22948824</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3uwm" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 4-~~hydroxy-3-methylbut-2-enyl diphosphate reductase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bacher, A]]	+	[[Category: Bacher A]]
-	[[Category: Eisenreich, W]]	+	[[Category: Eisenreich W]]
-	[[Category: Groll, M]]	+	[[Category: Groll M]]
-	[[Category: Oldfield, E]]	+	[[Category: Oldfield E]]
-	[[Category: Schulz, C]]	+	[[Category: Schulz C]]
-	[[Category: Span, I]]	+	[[Category: Span I]]
-	[[Category: Wang, K]]	+	[[Category: Wang K]]
-	[[Category: Wang, W]]	+	[[Category: Wang W]]
-	[[Category: Zhang, Y]]	+	[[Category: Zhang Y]]
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Iron-sulfur protein]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reductase~~]]	+

OCA at 08:12, 20 July 2022

OCA — Wed, 20 Jul 2022 08:12:40 GMT

←Older revision		Revision as of 08:12, 20 July 2022
Line 1:		Line 1:

	==Ec_IspH in complex with 4-oxobutyl diphosphate (1302)==		==Ec_IspH in complex with 4-oxobutyl diphosphate (1302)==
-	<StructureSection load='3uwm' size='340' side='right' caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3uwm' size='340' side='right'caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ke8\|3ke8]], [[3dnf\|3dnf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [~~http~~://pdbe.org/3uwm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uwm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [https://pdbe.org/3uwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [https://www.ebi.ac.uk/pdbsum/3uwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>	+	[[https://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]	+	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Bacher, A]]		[[Category: Bacher, A]]
	[[Category: Eisenreich, W]]		[[Category: Eisenreich, W]]

OCA at 12:55, 4 August 2016

OCA — Thu, 04 Aug 2016 12:55:20 GMT

←Older revision		Revision as of 12:55, 4 August 2016
Line 1:		Line 1:
		+
	==Ec_IspH in complex with 4-oxobutyl diphosphate (1302)==		==Ec_IspH in complex with 4-oxobutyl diphosphate (1302)==
	<StructureSection load='3uwm' size='340' side='right' caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3uwm' size='340' side='right' caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [http://www.ebi.ac.uk/pdbsum/3uwm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [http://pdbe.org/3uwm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [http://www.ebi.ac.uk/pdbsum/3uwm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uwm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Bacher, A]]		[[Category: Bacher, A]]
	[[Category: Eisenreich, W]]		[[Category: Eisenreich, W]]

OCA at 14:45, 24 December 2014

OCA — Wed, 24 Dec 2014 14:45:33 GMT

←Older revision		Revision as of 14:45, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWM FirstGlance]. <br>		<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [http://www.ebi.ac.uk/pdbsum/3uwm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [http://www.ebi.ac.uk/pdbsum/3uwm PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISPH_ECOLI ISPH_ECOLI]] Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth.<ref>PMID:19569147</ref> <ref>PMID:20080550</ref> <ref>PMID:22137895</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[4-hydroxy-3-methylbut-2-enyl diphosphate reductase\|4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Bacher, A.]]	+	[[Category: Bacher, A]]
-	[[Category: Eisenreich, W.]]	+	[[Category: Eisenreich, W]]
-	[[Category: Groll, M.]]	+	[[Category: Groll, M]]
-	[[Category: Oldfield, E.]]	+	[[Category: Oldfield, E]]
-	[[Category: Schulz, C.]]	+	[[Category: Schulz, C]]
-	[[Category: Span, I.]]	+	[[Category: Span, I]]
-	[[Category: Wang, K.]]	+	[[Category: Wang, K]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Cytosol]]		[[Category: Cytosol]]
	[[Category: Iron-sulfur protein]]		[[Category: Iron-sulfur protein]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Reductase]]		[[Category: Reductase]]

OCA at 06:54, 25 June 2014

OCA — Wed, 25 Jun 2014 06:54:58 GMT

←Older revision		Revision as of 06:54, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3uwm.~~png~~\|~~left~~\|~~200px~~]]	+	==Ec_IspH in complex with 4-oxobutyl diphosphate (1302)==
		+	<StructureSection load='3uwm' size='340' side='right' caption='[[3uwm]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UWM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0JX:(3E)-4-HYDROXYBUT-3-EN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0JX</scene>, <scene name='pdbligand=0K2:4-OXOBUTYL+TRIHYDROGEN+DIPHOSPHATE'>0K2</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ke8\|3ke8]], [[3dnf\|3dnf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ispH, lytB, yaaE, b0029, JW0027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxy-3-methylbut-2-enyl_diphosphate_reductase 4-hydroxy-3-methylbut-2-enyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.2 1.17.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uwm RCSB], [http://www.ebi.ac.uk/pdbsum/3uwm PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The final step of the methylerythritol phosphate isoprenoid biosynthesis pathway is catalysed by the iron-sulphur enzyme IspH, producing the universal precursors of terpenes: isopentenyl diphosphate and dimethylallyl diphosphate. Here we report an unforeseen reaction discovered during the investigation of the interaction of IspH with acetylene inhibitors by X-ray crystallography, Mossbauer, and nuclear magnetic resonance spectroscopy. In addition to its role as a 2H(+)/2e(-) reductase, IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition. The reactions only occur with the oxidised protein and proceed via eta(1)-O-enolate intermediates. One of these is characterized crystallographically and contains a C4 ligand oxygen bound to the unique, fourth iron in the 4Fe-4S cluster: this intermediate subsequently hydrolyzes to produce an aldehyde product. This unexpected side to IspH reactivity is of interest in the context of the mechanism of action of other acetylene hydratases, as well as in the design of antiinfectives targeting IspH.

-	~~{{STRUCTURE_3uwm\| PDB=3uwm \| SCENE= }}~~	+	Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.,Span I, Wang K, Wang W, Zhang Y, Bacher A, Eisenreich W, Li K, Schulz C, Oldfield E, Groll M Nat Commun. 2012 Sep 4;3:1042. doi: 10.1038/ncomms2052. PMID:22948824<ref>PMID:22948824</ref>

-	~~===Ec_IspH in complex with 4-oxobutyl diphosphate (1302)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22948824}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3uwm]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA].~~	+	</StructureSection>
	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]		[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 06:13, 19 September 2012

OCA — Wed, 19 Sep 2012 06:13:23 GMT

←Older revision		Revision as of 06:13, 19 September 2012
Line 1:		Line 1:
-	[[Image:3uwm.~~jpg~~\|left\|200px]]	+	[[Image:3uwm.png\|left\|200px]]

	{{STRUCTURE_3uwm\| PDB=3uwm \| SCENE= }}		{{STRUCTURE_3uwm\| PDB=3uwm \| SCENE= }}
Line 5:		Line 5:
	===Ec_IspH in complex with 4-oxobutyl diphosphate (1302)===		===Ec_IspH in complex with 4-oxobutyl diphosphate (1302)===

		+	{{ABSTRACT_PUBMED_22948824}}

	==About this Structure==		==About this Structure==

OCA at 05:32, 5 September 2012

OCA — Wed, 05 Sep 2012 05:32:04 GMT

←Older revision		Revision as of 05:32, 5 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uwm.jpg\|left\|200px]]

-	~~The entry~~ 3uwm ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3uwm\| PDB=3uwm \| SCENE= }}

-	~~Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.~~	+	===Ec_IspH in complex with 4-oxobutyl diphosphate (1302)===

-	~~Description: Ec_IspH in complex~~ with 4-~~oxobutyl~~ diphosphate ~~(1302)~~	+
		+	==About this Structure==
		+	[[3uwm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWM OCA].
		+	[[Category: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase]]
		+	[[Category: Escherichia coli]]
		+	[[Category: Bacher, A.]]
		+	[[Category: Eisenreich, W.]]
		+	[[Category: Groll, M.]]
		+	[[Category: Oldfield, E.]]
		+	[[Category: Schulz, C.]]
		+	[[Category: Span, I.]]
		+	[[Category: Wang, K.]]
		+	[[Category: Wang, W.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Cytosol]]
		+	[[Category: Iron-sulfur protein]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Reductase]]

OCA at 07:12, 28 March 2012

OCA — Wed, 28 Mar 2012 07:12:23 GMT

←Older revision		Revision as of 07:12, 28 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uwm is ON HOLD	+	The entry 3uwm is ON HOLD until Paper Publication

	Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.		Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.

	Description: Ec_IspH in complex with 4-oxobutyl diphosphate (1302)		Description: Ec_IspH in complex with 4-oxobutyl diphosphate (1302)

OCA: Protected "3uwm" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2011 11:13:22 GMT

Protected "3uwm" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:13, 14 December 2011

OCA: New page: '''Unreleased structure''' The entry 3uwm is ON HOLD Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M. Description: Ec_I...

OCA — Wed, 14 Dec 2011 11:13:20 GMT

New page: '''Unreleased structure''' The entry 3uwm is ON HOLD Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M. Description: Ec_I...

New page

'''Unreleased structure'''

The entry 3uwm is ON HOLD

Authors: Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.

Description: Ec_IspH in complex with 4-oxobutyl diphosphate (1302)