3uzj - Revision history

OCA at 14:12, 14 March 2024

OCA — Thu, 14 Mar 2024 14:12:09 GMT

←Older revision		Revision as of 14:12, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3uzj' size='340' side='right'caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='3uzj' size='340' side='right'caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=0CT:1H~~-~~BENZOTRIAZOLE'>0CT</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.69Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3uxa\|3uxa]]~~, ~~[[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [https://pdbe.org/3uzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [https://www.ebi.ac.uk/pdbsum/3uzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [https://pdbe.org/3uzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [https://www.ebi.ac.uk/pdbsum/3uzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzj ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Computational design is a test of our understanding of enzyme catalysis and a means of engineering novel, tailor-made enzymes. While the de novo computational design of catalytically efficient enzymes remains a challenge, designed enzymes may comprise unique starting points for further optimization by directed evolution. Directed evolution of two computationally designed Kemp eliminases, KE07 and KE70, led to low to moderately efficient enzymes (k(cat)/K(m) values of </= 5 10(4) M(-1)s(-1)). Here we describe the optimization of a third design, KE59. Although KE59 was the most catalytically efficient Kemp eliminase from this design series (by k(cat)/K(m), and by catalyzing the elimination of nonactivated benzisoxazoles), its impaired stability prevented its evolutionary optimization. To boost KE59's evolvability, stabilizing consensus mutations were included in the libraries throughout the directed evolution process. The libraries were also screened with less activated substrates. Sixteen rounds of mutation and selection led to > 2,000-fold increase in catalytic efficiency, mainly via higher k(cat) values. The best KE59 variants exhibited k(cat)/K(m) values up to 0.6 10(6) M(-1)s(-1), and k(cat)/k(uncat) values of </= 10(7) almost regardless of substrate reactivity. Biochemical, structural, and molecular dynamics (MD) simulation studies provided insights regarding the optimization of KE59. Overall, the directed evolution of three different designed Kemp eliminases, KE07, KE70, and KE59, demonstrates that computational designs are highly evolvable and can be optimized to high catalytic efficiencies.
-
-	Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.,Khersonsky O, Kiss G, Rothlisberger D, Dym O, Albeck S, Houk KN, Baker D, Tawfik DS Proc Natl Acad Sci U S A. 2012 Jun 8. PMID:22685214<ref>PMID:22685214</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3uzj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Albeck, S]]	+	[[Category: Albeck S]]
-	[[Category: Baker, D]]	+	[[Category: Baker D]]
-	[[Category: Dym, O]]	+	[[Category: Dym O]]
-	[[Category: Houk~~, K N]]~~	+	[[Category: Houk KN]]
-	~~[[Category: ISPC, Israel Structural Proteomics Center~~]]	+	[[Category: Khersonsky O]]
-	[[Category: Khersonsky, O]]	+	[[Category: Kiss G]]
-	[[Category: Kiss, G]]	+	[[Category: Roethlisberger D]]
-	[[Category: Roethlisberger, D]]	+	[[Category: Tawfik DS]]
-	[[Category: Tawfik~~, D S]]~~	+
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Lyase-lyase inhibitor complex]]~~	+
-	~~[[Category: Structural genomic~~]]	+

OCA at 08:18, 20 July 2022

OCA — Wed, 20 Jul 2022 08:18:15 GMT

←Older revision		Revision as of 08:18, 20 July 2022
Line 1:		Line 1:

	==Designed protein KE59 R13 3/11H with benzotriazole==		==Designed protein KE59 R13 3/11H with benzotriazole==
-	<StructureSection load='3uzj' size='340' side='right' caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>	+	<StructureSection load='3uzj' size='340' side='right'caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uxa\|3uxa]], [[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3uxa\|3uxa]], [[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [~~http~~://pdbe.org/3uzj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3uzj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [https://pdbe.org/3uzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [https://www.ebi.ac.uk/pdbsum/3uzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Albeck, S]]		[[Category: Albeck, S]]
	[[Category: Baker, D]]		[[Category: Baker, D]]

OCA at 21:50, 5 August 2016

OCA — Fri, 05 Aug 2016 21:50:32 GMT

←Older revision		Revision as of 21:50, 5 August 2016
Line 1:		Line 1:
		+
	==Designed protein KE59 R13 3/11H with benzotriazole==		==Designed protein KE59 R13 3/11H with benzotriazole==
	<StructureSection load='3uzj' size='340' side='right' caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='3uzj' size='340' side='right' caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uxa\|3uxa]], [[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uxa\|3uxa]], [[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [http://www.ebi.ac.uk/pdbsum/3uzj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [http://pdbe.org/3uzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [http://www.ebi.ac.uk/pdbsum/3uzj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uzj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3uzj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Albeck, S]]		[[Category: Albeck, S]]
	[[Category: Baker, D]]		[[Category: Baker, D]]

OCA at 07:17, 21 December 2014

OCA — Sun, 21 Dec 2014 07:17:32 GMT

←Older revision		Revision as of 07:17, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}~~	+	==Designed protein KE59 R13 3/11H with benzotriazole==
-	===Designed protein KE59 R13 3/11H with benzotriazole===	+	<StructureSection load='3uzj' size='340' side='right' caption='[[3uzj]], [[Resolution\|resolution]] 1.69Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22685214}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UZJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0CT:1H-BENZOTRIAZOLE'>0CT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uxa\|3uxa]], [[3uxd\|3uxd]], [[3uy7\|3uy7]], [[3uy8\|3uy8]], [[3uyc\|3uyc]], [[3uz5\|3uz5]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uzj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uzj RCSB], [http://www.ebi.ac.uk/pdbsum/3uzj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Computational design is a test of our understanding of enzyme catalysis and a means of engineering novel, tailor-made enzymes. While the de novo computational design of catalytically efficient enzymes remains a challenge, designed enzymes may comprise unique starting points for further optimization by directed evolution. Directed evolution of two computationally designed Kemp eliminases, KE07 and KE70, led to low to moderately efficient enzymes (k(cat)/K(m) values of </= 5 10(4) M(-1)s(-1)). Here we describe the optimization of a third design, KE59. Although KE59 was the most catalytically efficient Kemp eliminase from this design series (by k(cat)/K(m), and by catalyzing the elimination of nonactivated benzisoxazoles), its impaired stability prevented its evolutionary optimization. To boost KE59's evolvability, stabilizing consensus mutations were included in the libraries throughout the directed evolution process. The libraries were also screened with less activated substrates. Sixteen rounds of mutation and selection led to > 2,000-fold increase in catalytic efficiency, mainly via higher k(cat) values. The best KE59 variants exhibited k(cat)/K(m) values up to 0.6 10(6) M(-1)s(-1), and k(cat)/k(uncat) values of </= 10(7) almost regardless of substrate reactivity. Biochemical, structural, and molecular dynamics (MD) simulation studies provided insights regarding the optimization of KE59. Overall, the directed evolution of three different designed Kemp eliminases, KE07, KE70, and KE59, demonstrates that computational designs are highly evolvable and can be optimized to high catalytic efficiencies.

-	~~==About this Structure==~~	+	Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.,Khersonsky O, Kiss G, Rothlisberger D, Dym O, Albeck S, Houk KN, Baker D, Tawfik DS Proc Natl Acad Sci U S A. 2012 Jun 8. PMID:22685214<ref>PMID:22685214</ref>
-	~~[[3uzj]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3UZJ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Kemp eliminase\|Kemp eliminase]]		*[[Kemp eliminase\|Kemp eliminase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022685214<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Albeck, S.]]	+	[[Category: Albeck, S]]
-	[[Category: Baker, D.]]	+	[[Category: Baker, D]]
-	[[Category: Dym, O.]]	+	[[Category: Dym, O]]
-	[[Category: Houk, K N.]]	+	[[Category: Houk, K N]]
-	[[Category: ISPC, Israel Structural Proteomics Center.]]	+	[[Category: ISPC, Israel Structural Proteomics Center]]
-	[[Category: Khersonsky, O.]]	+	[[Category: Khersonsky, O]]
-	[[Category: Kiss, G.]]	+	[[Category: Kiss, G]]
-	[[Category: Roethlisberger, D.]]	+	[[Category: Roethlisberger, D]]
-	[[Category: Tawfik, D S.]]	+	[[Category: Tawfik, D S]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
-	[[Category: ISPC]]
-	[[Category: Israel Structural Proteomics Center]]
	[[Category: Lyase-lyase inhibitor complex]]		[[Category: Lyase-lyase inhibitor complex]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 10:52, 20 March 2013

OCA — Wed, 20 Mar 2013 10:52:26 GMT

←Older revision		Revision as of 10:52, 20 March 2013
Line 7:		Line 7:

	==See Also==		==See Also==
-	*[[Kemp eliminase ~~(KE07)~~\|Kemp eliminase ~~(KE07)~~]]	+	*[[Kemp eliminase\|Kemp eliminase]]

	==Reference==		==Reference==

OCA at 10:33, 11 March 2013

OCA — Mon, 11 Mar 2013 10:33:23 GMT

←Older revision		Revision as of 10:33, 11 March 2013
Line 1:		Line 1:
-	[[Image:3uzj.png\|left\|200px]]
-
	{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}		{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}
-
	===Designed protein KE59 R13 3/11H with benzotriazole===		===Designed protein KE59 R13 3/11H with benzotriazole===
-
	{{ABSTRACT_PUBMED_22685214}}		{{ABSTRACT_PUBMED_22685214}}

	==About this Structure==		==About this Structure==
	[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA].		[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA].
		+
		+	==See Also==
		+	*[[Kemp eliminase (KE07)\|Kemp eliminase (KE07)]]

	==Reference==		==Reference==

OCA at 04:53, 27 June 2012

OCA — Wed, 27 Jun 2012 04:53:29 GMT

←Older revision		Revision as of 04:53, 27 June 2012
Line 1:		Line 1:
-	[[Image:3uzj.~~jpg~~\|left\|200px]]	+	[[Image:3uzj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3uzj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}		{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}

	===Designed protein KE59 R13 3/11H with benzotriazole===		===Designed protein KE59 R13 3/11H with benzotriazole===

		+	{{ABSTRACT_PUBMED_22685214}}

	==About this Structure==		==About this Structure==
	[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA].		[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022685214</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Albeck, S.]]		[[Category: Albeck, S.]]

OCA at 07:52, 6 June 2012

OCA — Wed, 06 Jun 2012 07:52:10 GMT

←Older revision		Revision as of 07:52, 6 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3uzj.jpg\|left\|200px]]

-	The ~~entry 3uzj~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3uzj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3uzj\| PDB=3uzj \| SCENE= }}

-	~~Authors: Khersonsky, O., Kiss, G., Roethlisberger, D., Dym, O., Albeck, S., Houk, K.N., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)~~	+	===Designed protein KE59 R13 3/11H with benzotriazole===

-	~~Description~~: ~~Designed protein KE59 R13 3~~/~~11H with benzotriazole~~	+
		+	==About this Structure==
		+	[[3uzj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UZJ OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Albeck, S.]]
		+	[[Category: Baker, D.]]
		+	[[Category: Dym, O.]]
		+	[[Category: Houk, K N.]]
		+	[[Category: ISPC, Israel Structural Proteomics Center.]]
		+	[[Category: Khersonsky, O.]]
		+	[[Category: Kiss, G.]]
		+	[[Category: Roethlisberger, D.]]
		+	[[Category: Tawfik, D S.]]
		+	[[Category: Beta barrel]]
		+	[[Category: ISPC]]
		+	[[Category: Israel Structural Proteomics Center]]
		+	[[Category: Lyase-lyase inhibitor complex]]
		+	[[Category: Structural genomic]]

OCA at 06:46, 28 December 2011

OCA — Wed, 28 Dec 2011 06:46:05 GMT

←Older revision		Revision as of 06:46, 28 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3uzj is ON HOLD	+	The entry 3uzj is ON HOLD until Paper Publication

-	Authors: ~~Olga~~ Khersonsky, ~~Gert~~ Kiss, ~~Daniela R thlisberger~~, ~~Orly~~ Dym, ~~Shira~~ Albeck, K. N. ~~Houk~~, ~~David~~ Baker, ~~Dan S~~. Tawfik, Israel Structural Proteomics Center (ISPC)	+	Authors: Khersonsky, O., Kiss, G., Roethlisberger, D., Dym, O., Albeck, S., Houk, K.N., Baker, D., Tawfik, D.S., Israel Structural Proteomics Center (ISPC)

-	Description: ~~KE59~~ Designed protein	+	Description: Designed protein KE59 R13 3/11H with benzotriazole

OCA: Protected "3uzj" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2011 11:16:53 GMT

Protected "3uzj" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:16, 14 December 2011