3v0j - Revision history

OCA at 14:13, 14 March 2024

OCA — Thu, 14 Mar 2024 14:13:38 GMT

←Older revision		Revision as of 14:13, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3v0j' size='340' side='right'caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='3v0j' size='340' side='right'caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ascidia_intestinalis Ascidia~~ intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.72Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Ci-VSP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [https://pdbe.org/3v0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [https://www.ebi.ac.uk/pdbsum/3v0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [https://pdbe.org/3v0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [https://www.ebi.ac.uk/pdbsum/3v0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0j ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q4W8A1_CIOIN Q4W8A1_CIOIN]
-	The Ciona intestinalis voltage-sensing phosphatase (Ci-VSP) couples a voltage-sensing domain (VSD) to a lipid phosphatase that is similar to the tumor suppressor PTEN. How the VSD controls enzyme function has been unclear. Here, we present high-resolution crystal structures of the Ci-VSP enzymatic domain that reveal conformational changes in a crucial loop, termed the 'gating loop', that controls access to the active site by a mechanism in which residue Glu411 directly competes with substrate. Structure-based mutations that restrict gating loop conformation impair catalytic function and demonstrate that Glu411 also contributes to substrate selectivity. Structure-guided mutations further define an interaction between the gating loop and linker that connects the phosphatase to the VSD for voltage control of enzyme activity. Together, the data suggest that functional coupling between the gating loop and the linker forms the heart of the regulatory mechanism that controls voltage-dependent enzyme activation.	+
-		+
-	~~A glutamate switch controls voltage-sensitive phosphatase function.,Liu L, Kohout SC, Xu Q, Muller S, Kimberlin CR, Isacoff EY, Minor DL Jr Nat Struct Mol Biol. 2012 May 6;19(6)~~:~~633-41. doi: 10.1038~~/~~nsmb.2289. PMID:22562138<ref>PMID:22562138<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3v0j" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ascidia~~ intestinalis]]	+	[[Category: Ciona intestinalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Isacoff~~, E Y~~]]	+	[[Category: Isacoff EY]]
-	[[Category: Kimberlin, C]]	+	[[Category: Kimberlin C]]
-	[[Category: Kohout~~, S C~~]]	+	[[Category: Kohout SC]]
-	[[Category: Liu, L]]	+	[[Category: Liu L]]
-	[[Category: Minor~~, D L~~]]	+	[[Category: Minor DL]]
-	[[Category: Muller, S]]	+	[[Category: Muller S]]
-	[[Category: Xu, Q]]	+	[[Category: Xu Q]]
-	~~[[Category: C2]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Ptp~~]]	+

OCA at 08:20, 20 July 2022

OCA — Wed, 20 Jul 2022 08:20:51 GMT

←Older revision		Revision as of 08:20, 20 July 2022
Line 1:		Line 1:

	==Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405==		==Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405==
-	<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>	+	<StructureSection load='3v0j' size='340' side='right'caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ascidia_intestinalis Ascidia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascidia_intestinalis Ascidia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [~~http~~://pdbe.org/3v0j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v0j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [https://pdbe.org/3v0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [https://www.ebi.ac.uk/pdbsum/3v0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Ascidia intestinalis]]		[[Category: Ascidia intestinalis]]
		+	[[Category: Large Structures]]
	[[Category: Isacoff, E Y]]		[[Category: Isacoff, E Y]]
	[[Category: Kimberlin, C]]		[[Category: Kimberlin, C]]

OCA at 14:05, 11 August 2016

OCA — Thu, 11 Aug 2016 14:05:17 GMT

←Older revision		Revision as of 14:05, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405==		==Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405==
	<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [http://www.ebi.ac.uk/pdbsum/3v0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [http://pdbe.org/3v0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [http://www.ebi.ac.uk/pdbsum/3v0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3v0j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:53, 20 January 2015

OCA — Tue, 20 Jan 2015 11:53:04 GMT

←Older revision		Revision as of 11:53, 20 January 2015
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	</StructureSection>		</StructureSection>
	[[Category: Ascidia intestinalis]]		[[Category: Ascidia intestinalis]]
-	[[Category: Isacoff, E Y.]]	+	[[Category: Isacoff, E Y]]
-	[[Category: Kimberlin, C.]]	+	[[Category: Kimberlin, C]]
-	[[Category: Kohout, S C.]]	+	[[Category: Kohout, S C]]
-	[[Category: Liu, L.]]	+	[[Category: Liu, L]]
-	[[Category: Minor, D L.]]	+	[[Category: Minor, D L]]
-	[[Category: Muller, S.]]	+	[[Category: Muller, S]]
-	[[Category: Xu, Q.]]	+	[[Category: Xu, Q]]
	[[Category: C2]]		[[Category: C2]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphatase]]		[[Category: Phosphatase]]
	[[Category: Ptp]]		[[Category: Ptp]]

OCA at 05:53, 8 October 2014

OCA — Wed, 08 Oct 2014 05:53:07 GMT

←Older revision		Revision as of 05:53, 8 October 2014
Line 2:		Line 2:
	<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ciona_intestinalis Ciona~~ intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ascidia_intestinalis Ascidia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V0J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 ~~Ciona~~ intestinalis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ascidia intestinalis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [http://www.ebi.ac.uk/pdbsum/3v0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [http://www.ebi.ac.uk/pdbsum/3v0j PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	A glutamate switch controls voltage-sensitive phosphatase function.,Liu L, Kohout SC, Xu Q, Muller S, Kimberlin CR, Isacoff EY, Minor DL Jr Nat Struct Mol Biol. 2012 May 6;19(6):633-41. doi: 10.1038/nsmb.2289. PMID:22562138<ref>PMID:22562138</ref>		A glutamate switch controls voltage-sensitive phosphatase function.,Liu L, Kohout SC, Xu Q, Muller S, Kimberlin CR, Isacoff EY, Minor DL Jr Nat Struct Mol Biol. 2012 May 6;19(6):633-41. doi: 10.1038/nsmb.2289. PMID:22562138<ref>PMID:22562138</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ciona~~ intestinalis]]	+	[[Category: Ascidia intestinalis]]
	[[Category: Isacoff, E Y.]]		[[Category: Isacoff, E Y.]]
	[[Category: Kimberlin, C.]]		[[Category: Kimberlin, C.]]
	[[Category: Kohout, S C.]]		[[Category: Kohout, S C.]]
	[[Category: Liu, L.]]		[[Category: Liu, L.]]
-	[[Category: Minor, D L]]	+	[[Category: Minor, D L.]]
	[[Category: Muller, S.]]		[[Category: Muller, S.]]
	[[Category: Xu, Q.]]		[[Category: Xu, Q.]]

OCA at 06:44, 5 June 2014

OCA — Thu, 05 Jun 2014 06:44:29 GMT

←Older revision		Revision as of 06:44, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3v0j.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405==
		+	<StructureSection load='3v0j' size='340' side='right' caption='[[3v0j]], [[Resolution\|resolution]] 1.72Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V0J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v0d\|3v0d]], [[3v0e\|3v0e]], [[3v0f\|3v0f]], [[3v0g\|3v0g]], [[3v0h\|3v0h]], [[3v0i\|3v0i]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ci-VSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7719 Ciona intestinalis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v0j RCSB], [http://www.ebi.ac.uk/pdbsum/3v0j PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ciona intestinalis voltage-sensing phosphatase (Ci-VSP) couples a voltage-sensing domain (VSD) to a lipid phosphatase that is similar to the tumor suppressor PTEN. How the VSD controls enzyme function has been unclear. Here, we present high-resolution crystal structures of the Ci-VSP enzymatic domain that reveal conformational changes in a crucial loop, termed the 'gating loop', that controls access to the active site by a mechanism in which residue Glu411 directly competes with substrate. Structure-based mutations that restrict gating loop conformation impair catalytic function and demonstrate that Glu411 also contributes to substrate selectivity. Structure-guided mutations further define an interaction between the gating loop and linker that connects the phosphatase to the VSD for voltage control of enzyme activity. Together, the data suggest that functional coupling between the gating loop and the linker forms the heart of the regulatory mechanism that controls voltage-dependent enzyme activation.

-	~~{{STRUCTURE_3v0j\| PDB=3v0j \| SCENE= }}~~	+	A glutamate switch controls voltage-sensitive phosphatase function.,Liu L, Kohout SC, Xu Q, Muller S, Kimberlin CR, Isacoff EY, Minor DL Jr Nat Struct Mol Biol. 2012 May 6;19(6):633-41. doi: 10.1038/nsmb.2289. PMID:22562138<ref>PMID:22562138</ref>

-	~~===Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP)~~, ~~residues 241-576(C363S), Deletion~~ of ~~401-405===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22562138}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3v0j]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Ciona_intestinalis Ciona intestinalis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022562138<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Ciona intestinalis]]		[[Category: Ciona intestinalis]]
	[[Category: Isacoff, E Y.]]		[[Category: Isacoff, E Y.]]

OCA at 06:27, 25 July 2012

OCA — Wed, 25 Jul 2012 06:27:02 GMT

←Older revision		Revision as of 06:27, 25 July 2012
Line 1:		Line 1:
-	[[Image:3v0j.~~jpg~~\|left\|200px]]	+	[[Image:3v0j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3v0j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3v0j\| PDB=3v0j \| SCENE= }}		{{STRUCTURE_3v0j\| PDB=3v0j \| SCENE= }}

	===Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405===		===Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405===

		+	{{ABSTRACT_PUBMED_22562138}}

	==About this Structure==		==About this Structure==
	[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA].		[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022562138</ref><references group="xtra"/>
	[[Category: Ciona intestinalis]]		[[Category: Ciona intestinalis]]
	[[Category: Isacoff, E Y.]]		[[Category: Isacoff, E Y.]]

OCA at 09:23, 9 May 2012

OCA — Wed, 09 May 2012 09:23:34 GMT

←Older revision		Revision as of 09:23, 9 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3v0j.jpg\|left\|200px]]

-	The ~~entry 3v0j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3v0j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3v0j\| PDB=3v0j \| SCENE= }}

-	~~Authors: Liu~~, L., ~~Kohout, S.C., Xu, Q., Muller, S., Kimberlin, C., Isacoff, E.Y., Minor, D.L~~	+	===Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405===

-	~~Description: Crystal~~ structure of Ciona intestinalis ~~voltage sensor~~-~~containing phosphatase (Ci-VSP)~~, ~~residues 241-576(C363S)~~, ~~Deletion of 401-405~~	+
		+	==About this Structure==
		+	[[3v0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0J OCA].
		+	[[Category: Ciona intestinalis]]
		+	[[Category: Isacoff, E Y.]]
		+	[[Category: Kimberlin, C.]]
		+	[[Category: Kohout, S C.]]
		+	[[Category: Liu, L.]]
		+	[[Category: Minor, D L]]
		+	[[Category: Muller, S.]]
		+	[[Category: Xu, Q.]]
		+	[[Category: C2]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphatase]]
		+	[[Category: Ptp]]

OCA at 06:46, 28 December 2011

OCA — Wed, 28 Dec 2011 06:46:19 GMT

←Older revision		Revision as of 06:46, 28 December 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v0j is ON HOLD	+	The entry 3v0j is ON HOLD until Paper Publication

	Authors: Liu, L., Kohout, S.C., Xu, Q., Muller, S., Kimberlin, C., Isacoff, E.Y., Minor, D.L		Authors: Liu, L., Kohout, S.C., Xu, Q., Muller, S., Kimberlin, C., Isacoff, E.Y., Minor, D.L

	Description: Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405		Description: Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), Deletion of 401-405

OCA: Protected "3v0j" [edit=sysop:move=sysop]

OCA — Wed, 14 Dec 2011 11:17:57 GMT

Protected "3v0j" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:17, 14 December 2011