3v1m - Revision history

OCA at 10:29, 1 March 2024

OCA — Fri, 01 Mar 2024 10:29:44 GMT

←Older revision		Revision as of 10:29, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3v1m' size='340' side='right'caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='3v1m' size='340' side='right'caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Burkholderia_cepacia_lb400 Burkholderia cepacia lb400~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paraburkholderia_xenovorans_LB400 Paraburkholderia xenovorans LB400]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HPK:(3E)~~-2,~~6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.92Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bphD, Bxeno_C1120, Bxe_C1186 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=266265 Burkholderia cepacia LB400])</td></tr>~~	+
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3~~-~~enoate_hydrolase~~ 2,6-~~dioxo~~-6-~~phenylhexa~~-3-~~enoate hydrolase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [https://pdbe.org/3v1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [https://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [https://pdbe.org/3v1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [https://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~BPHD_BURXL BPHD_BURXL]~~] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>	+	[https://www.uniprot.org/uniprot/BPHD_PARXL BPHD_PARXL] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Meta-cleavage product (MCP) hydrolases are members of the alpha/beta-hydrolase superfamily that utilize a Ser-His-Asp triad to catalyze the hydrolysis of a C-C bond. BphD, the MCP hydrolase from the biphenyl degradation pathway, hydrolyzes 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to 2-hydroxypenta-2,4-dienoic acid (HPD) and benzoate. A 1.6 A resolution crystal structure of BphD H265Q incubated with HOPDA revealed that the enzyme's catalytic serine was benzoylated. The acyl-enzyme is stabilized by hydrogen bonding from the amide backbone of 'oxyanion hole' residues, consistent with formation of a tetrahedral oxyanion during nucleophilic attack by Ser112. Chemical quench and mass spectrometry studies substantiated the formation and decay of a Ser112-benzoyl species in wild-type BphD on a time scale consistent with turnover and incorporation of a single equivalent of (18)O into the benzoate produced during hydrolysis in H(2)(18)O. Rapid-scanning kinetic studies indicated that the catalytic histidine contributes to the rate of acylation by only an order of magnitude, but affects the rate of deacylation by over 5 orders of magnitude. The orange-colored catalytic intermediate, ES(red), previously detected in the wild-type enzyme and proposed herein to be a carbanion, was not observed during hydrolysis by H265Q. In the newly proposed mechanism, the carbanion abstracts a proton from Ser112, thereby completing tautomerization and generating a serinate for nucleophilic attack on the C6-carbonyl. Finally, quantification of an observed pre-steady-state kinetic burst suggests that BphD is a half-site reactive enzyme. While the updated catalytic mechanism shares features with the serine proteases, MCP hydrolase-specific chemistry highlights the versatility of the Ser-His-Asp triad.	+
-		+
-	Identification of an Acyl-Enzyme Intermediate in a meta-Cleavage Product Hydrolase Reveals the Versatility of the Catalytic Triad.,Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD J Am Chem Soc. 2012 Mar 14;134(10):4615-24. Epub 2012 Mar 5. PMID:22339283<ref>PMID:22339283</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3v1m" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
-	[[Category: Burkholderia cepacia lb400]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bolin, J T~~]]	+	[[Category: Paraburkholderia xenovorans LB400]]
-	[[Category: ~~Ghosh, S~~]]	+	[[Category: Bolin JT]]
-	[[Category: ~~2-hydroxy-6-oxo-6-phenyl-hexa-2]]~~	+	[[Category: Ghosh S]]
-	~~[[Category: 4-dienoate hydrolase]]~~	+
-	~~[[Category: Alpha/beta hydrolase]]~~	+
-	~~[[Category: Alpha/beta hydrolase fold]]~~	+
-	~~[[Category: Bphd]]~~	+
-	~~[[Category: C-c bond hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mcp hydrolase]]~~	+
-	~~[[Category: Meta cleavage product hydrolase]]~~	+
-	~~[[Category: Pcb degradation~~]]	+

OCA at 08:23, 20 July 2022

OCA — Wed, 20 Jul 2022 08:23:24 GMT

←Older revision		Revision as of 08:23, 20 July 2022
Line 1:		Line 1:

	==Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA==		==Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA==
-	<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>	+	<StructureSection load='3v1m' size='340' side='right'caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Burkholderia_cepacia_lb400 Burkholderia cepacia lb400]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cepacia_lb400 Burkholderia cepacia lb400]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266265 Burkholderia cepacia LB400])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266265 Burkholderia cepacia LB400])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [~~http~~://pdbe.org/3v1m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [https://pdbe.org/3v1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [https://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>	+	[[https://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]		[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
	[[Category: Burkholderia cepacia lb400]]		[[Category: Burkholderia cepacia lb400]]
		+	[[Category: Large Structures]]
	[[Category: Bolin, J T]]		[[Category: Bolin, J T]]
	[[Category: Ghosh, S]]		[[Category: Ghosh, S]]

OCA at 10:10, 8 November 2017

OCA — Wed, 08 Nov 2017 10:10:57 GMT

←Older revision		Revision as of 10:10, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burxl Burxl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia_lb400 Burkholderia cepacia lb400]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266265 ~~BURXL~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266265 Burkholderia cepacia LB400])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://pdbe.org/3v1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://pdbe.org/3v1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]		[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
-	[[Category: ~~Burxl~~]]	+	[[Category: Burkholderia cepacia lb400]]
	[[Category: Bolin, J T]]		[[Category: Bolin, J T]]
	[[Category: Ghosh, S]]		[[Category: Ghosh, S]]

OCA at 13:37, 4 August 2016

OCA — Thu, 04 Aug 2016 13:37:42 GMT

←Older revision		Revision as of 13:37, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA==		==Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA==
	<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>		<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Burkholderia_xenovorans Burkholderia xenovorans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burxl Burxl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~36873 Burkholderia xenovorans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266265 BURXL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://pdbe.org/3v1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3v1m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]		[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
-	[[Category: ~~Burkholderia xenovorans~~]]	+	[[Category: Burxl]]
	[[Category: Bolin, J T]]		[[Category: Bolin, J T]]
	[[Category: Ghosh, S]]		[[Category: Ghosh, S]]

OCA at 15:07, 24 December 2014

OCA — Wed, 24 Dec 2014 15:07:34 GMT

←Older revision		Revision as of 15:07, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>		<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BPHD_BURXL BPHD_BURXL]] Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).<ref>PMID:16964968</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Identification of an Acyl-Enzyme Intermediate in a meta-Cleavage Product Hydrolase Reveals the Versatility of the Catalytic Triad.,Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD J Am Chem Soc. 2012 Mar 14;134(10):4615-24. Epub 2012 Mar 5. PMID:22339283<ref>PMID:22339283</ref>		Identification of an Acyl-Enzyme Intermediate in a meta-Cleavage Product Hydrolase Reveals the Versatility of the Catalytic Triad.,Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD J Am Chem Soc. 2012 Mar 14;134(10):4615-24. Epub 2012 Mar 5. PMID:22339283<ref>PMID:22339283</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 25:
	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]		[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]
-	[[Category: Bolin, J T.]]	+	[[Category: Bolin, J T]]
-	[[Category: Ghosh, S.]]	+	[[Category: Ghosh, S]]
	[[Category: 2-hydroxy-6-oxo-6-phenyl-hexa-2]]		[[Category: 2-hydroxy-6-oxo-6-phenyl-hexa-2]]
	[[Category: 4-dienoate hydrolase]]		[[Category: 4-dienoate hydrolase]]

OCA at 05:55, 5 June 2014

OCA — Thu, 05 Jun 2014 05:55:20 GMT

←Older revision		Revision as of 05:55, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3v1m.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA==
		+	<StructureSection load='3v1m' size='340' side='right' caption='[[3v1m]], [[Resolution\|resolution]] 1.92Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPK:(3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE'>HPK</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2og1\|2og1]], [[2ri6\|2ri6]], [[2pu7\|2pu7]], [[2puh\|2puh]], [[2puj\|2puj]], [[2rhw\|2rhw]], [[2rht\|2rht]], [[3v1k\|3v1k]], [[3v1l\|3v1l]], [[3v1n\|3v1n]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bphD, Bxeno_C1120, Bxe_C1186 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36873 Burkholderia xenovorans])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,6-dioxo-6-phenylhexa-3-enoate_hydrolase 2,6-dioxo-6-phenylhexa-3-enoate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.8 3.7.1.8] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1m RCSB], [http://www.ebi.ac.uk/pdbsum/3v1m PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Meta-cleavage product (MCP) hydrolases are members of the alpha/beta-hydrolase superfamily that utilize a Ser-His-Asp triad to catalyze the hydrolysis of a C-C bond. BphD, the MCP hydrolase from the biphenyl degradation pathway, hydrolyzes 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to 2-hydroxypenta-2,4-dienoic acid (HPD) and benzoate. A 1.6 A resolution crystal structure of BphD H265Q incubated with HOPDA revealed that the enzyme's catalytic serine was benzoylated. The acyl-enzyme is stabilized by hydrogen bonding from the amide backbone of 'oxyanion hole' residues, consistent with formation of a tetrahedral oxyanion during nucleophilic attack by Ser112. Chemical quench and mass spectrometry studies substantiated the formation and decay of a Ser112-benzoyl species in wild-type BphD on a time scale consistent with turnover and incorporation of a single equivalent of (18)O into the benzoate produced during hydrolysis in H(2)(18)O. Rapid-scanning kinetic studies indicated that the catalytic histidine contributes to the rate of acylation by only an order of magnitude, but affects the rate of deacylation by over 5 orders of magnitude. The orange-colored catalytic intermediate, ES(red), previously detected in the wild-type enzyme and proposed herein to be a carbanion, was not observed during hydrolysis by H265Q. In the newly proposed mechanism, the carbanion abstracts a proton from Ser112, thereby completing tautomerization and generating a serinate for nucleophilic attack on the C6-carbonyl. Finally, quantification of an observed pre-steady-state kinetic burst suggests that BphD is a half-site reactive enzyme. While the updated catalytic mechanism shares features with the serine proteases, MCP hydrolase-specific chemistry highlights the versatility of the Ser-His-Asp triad.

-	<!--	+	Identification of an Acyl-Enzyme Intermediate in a meta-Cleavage Product Hydrolase Reveals the Versatility of the Catalytic Triad.,Ruzzini AC, Ghosh S, Horsman GP, Foster LJ, Bolin JT, Eltis LD J Am Chem Soc. 2012 Mar 14;134(10):4615-24. Epub 2012 Mar 5. PMID:22339283<ref>PMID:22339283</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3v1m", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3v1m\| PDB=3v1m \| SCENE= }}~~	+

-	~~===Crystal Structure of the S112A~~/~~H265Q mutant~~ of ~~a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22339283}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22339283 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22339283}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3v1m]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022339283<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]		[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
	[[Category: Burkholderia xenovorans]]		[[Category: Burkholderia xenovorans]]

OCA at 06:21, 11 April 2012

OCA — Wed, 11 Apr 2012 06:21:16 GMT

←Older revision		Revision as of 06:21, 11 April 2012
Line 1:		Line 1:
-	[[Image:3v1m.~~jpg~~\|left\|200px]]	+	[[Image:3v1m.png\|left\|200px]]

	<!--		<!--

OCA at 07:34, 21 March 2012

OCA — Wed, 21 Mar 2012 07:34:26 GMT

←Older revision		Revision as of 07:34, 21 March 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3v1m.jpg\|left\|200px]]

-	The ~~entry 3v1m~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3v1m", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3v1m\| PDB=3v1m \| SCENE= }}

-	~~Authors: Ghosh~~, S., ~~Bolin, J.T.~~	+	===Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA===

-	~~Description: Crystal Structure of~~ the ~~S112A~~/~~H265Q mutant of~~ a C-~~C hydrolase~~, ~~BphD from~~ Burkholderia xenovorans ~~LB400~~, ~~after exposure to its substrate HOPDA~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22339283}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22339283 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22339283}}
		+
		+	==About this Structure==
		+	[[3v1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_xenovorans Burkholderia xenovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1M OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022339283</ref><references group="xtra"/>
		+	[[Category: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase]]
		+	[[Category: Burkholderia xenovorans]]
		+	[[Category: Bolin, J T.]]
		+	[[Category: Ghosh, S.]]
		+	[[Category: 2-hydroxy-6-oxo-6-phenyl-hexa-2]]
		+	[[Category: 4-dienoate hydrolase]]
		+	[[Category: Alpha/beta hydrolase]]
		+	[[Category: Alpha/beta hydrolase fold]]
		+	[[Category: Bphd]]
		+	[[Category: C-c bond hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mcp hydrolase]]
		+	[[Category: Meta cleavage product hydrolase]]
		+	[[Category: Pcb degradation]]

OCA at 05:57, 11 January 2012

OCA — Wed, 11 Jan 2012 05:57:33 GMT

←Older revision		Revision as of 05:57, 11 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v1m is ON HOLD	+	The entry 3v1m is ON HOLD until Paper Publication

	Authors: Ghosh, S., Bolin, J.T.		Authors: Ghosh, S., Bolin, J.T.

	Description: Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA		Description: Crystal Structure of the S112A/H265Q mutant of a C-C hydrolase, BphD from Burkholderia xenovorans LB400, after exposure to its substrate HOPDA

OCA: Protected "3v1m" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2011 06:47:09 GMT

Protected "3v1m" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:47, 28 December 2011