3v1x - Revision history

OCA at 14:15, 14 March 2024

OCA — Thu, 14 Mar 2024 14:15:01 GMT

←Older revision		Revision as of 14:15, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3v1x' size='340' side='right'caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='3v1x' size='340' side='right'caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Strco Strco~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1X FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=0FV:(2Z)~~-~~2-FLUORO-3~~,~~7-DIMETHYLOCTA-2,6-DIEN-~~1~~-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.955Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3v1v\|3v1v]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [https://pdbe.org/3v1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [https://www.ebi.ac.uk/pdbsum/3v1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [https://pdbe.org/3v1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [https://www.ebi.ac.uk/pdbsum/3v1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).	+	[https://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The crystal structure of 2-methylisoborneol synthase (MIBS) from Streptomyces coelicolor A3(2) has been determined in complex with substrate analogues geranyl-S-thiolodiphosphate and 2-fluorogeranyl diphosphate at 1.80 and 1.95 A resolution, respectively. This terpenoid cyclase catalyzes the cyclization of the naturally occurring, noncanonical C-methylated isoprenoid substrate, 2-methylgeranyl diphosphate, to form the bicyclic product 2-methylisoborneol, a volatile C(11) homoterpene alcohol with an earthy, musty odor. While MIBS adopts the tertiary structure of a class I terpenoid cyclase, its dimeric quaternary structure differs from that previously observed in dimeric terpenoid cyclases from plants and fungi. The quaternary structure of MIBS is nonetheless similar in some respects to that of dimeric farnesyl diphosphate synthase, which is not a cyclase. The structures of MIBS complexed with substrate analogues provide insights regarding differences in the catalytic mechanism of MIBS and the mechanisms of (+)-bornyl diphosphate synthase and endo-fenchol synthase, plant cyclases that convert geranyl diphosphate into products with closely related bicyclic bornyl skeletons, but distinct structures and stereochemistries.	+
-		+
-	Structure of 2-Methylisoborneol Synthase from Streptomyces coelicolor and Implications for the Cyclization of a Noncanonical C-Methylated Monoterpenoid Substrate.,Koksal M, Chou WK, Cane DE, Christianson DW Biochemistry. 2012 Apr 10;51(14):3011-20. Epub 2012 Mar 28. PMID:22455514<ref>PMID:22455514</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3v1x" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Strco]]~~	+	[[Category: Christianson DW]]
-	[[Category: Christianson~~, D W~~]]	+	[[Category: Koksal M]]
-	[[Category: Koksal, M]]	+
-	~~[[Category: 2-methylisoborneol biosynthesis]]~~	+
-	~~[[Category: Biosynthesis of 2-methylisoborneol]]~~	+
-	~~[[Category: Class i terpenoid cyclase fold]]~~	+
-	~~[[Category: Ddxxxxd motif]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Ndxxsxxxe motif~~]]	+

OCA at 08:24, 20 July 2022

OCA — Wed, 20 Jul 2022 08:24:13 GMT

←Older revision		Revision as of 08:24, 20 July 2022
Line 1:		Line 1:

	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate==		==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate==
-	<StructureSection load='3v1x' size='340' side='right' caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>	+	<StructureSection load='3v1x' size='340' side='right'caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V1X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3v1v\|3v1v]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [~~http~~://pdbe.org/3v1x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v1x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [https://pdbe.org/3v1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [https://www.ebi.ac.uk/pdbsum/3v1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).	+	[[https://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Strco]]		[[Category: Strco]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]

OCA at 18:24, 4 August 2016

OCA — Thu, 04 Aug 2016 18:24:31 GMT

←Older revision		Revision as of 18:24, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate==		==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate==
	<StructureSection load='3v1x' size='340' side='right' caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='3v1x' size='340' side='right' caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces coelicolor~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strco Strco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1902 Streptomyces coelicolor~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100226 STRCO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [http://www.ebi.ac.uk/pdbsum/3v1x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [http://pdbe.org/3v1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [http://www.ebi.ac.uk/pdbsum/3v1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v1x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3v1x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces coelicolor~~]]	+	[[Category: Strco]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]
	[[Category: Koksal, M]]		[[Category: Koksal, M]]

OCA at 18:10, 24 December 2014

OCA — Wed, 24 Dec 2014 18:10:34 GMT

←Older revision		Revision as of 18:10, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1X FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [http://www.ebi.ac.uk/pdbsum/3v1x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [http://www.ebi.ac.uk/pdbsum/3v1x PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MIBS_STRCO MIBS_STRCO]] Catalyzes the cyclization of 2-methylgeranyl diphosphate (2-MeGPP) to 2-methylisoborneol (2-MIB), which likely involves the intermediacy of 2-methyllinalyl diphosphate. Is also able to catalyze the cyclization of geranyl diphosphate (GPP), albeit with much lower efficiency, leading to the formation of a complex mixture of cyclic monoterpenes, consisting of alpha-pinene (6%), beta-pinene (23%), limonene (32%), gamma-terpinene (29%), and delta-terpinene (10%).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Structure of 2-Methylisoborneol Synthase from Streptomyces coelicolor and Implications for the Cyclization of a Noncanonical C-Methylated Monoterpenoid Substrate.,Koksal M, Chou WK, Cane DE, Christianson DW Biochemistry. 2012 Apr 10;51(14):3011-20. Epub 2012 Mar 28. PMID:22455514<ref>PMID:22455514</ref>		Structure of 2-Methylisoborneol Synthase from Streptomyces coelicolor and Implications for the Cyclization of a Noncanonical C-Methylated Monoterpenoid Substrate.,Koksal M, Chou WK, Cane DE, Christianson DW Biochemistry. 2012 Apr 10;51(14):3011-20. Epub 2012 Mar 28. PMID:22455514<ref>PMID:22455514</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Christianson, D W.]]	+	[[Category: Christianson, D W]]
-	[[Category: Koksal, M.]]	+	[[Category: Koksal, M]]
	[[Category: 2-methylisoborneol biosynthesis]]		[[Category: 2-methylisoborneol biosynthesis]]
	[[Category: Biosynthesis of 2-methylisoborneol]]		[[Category: Biosynthesis of 2-methylisoborneol]]

OCA at 06:13, 5 June 2014

OCA — Thu, 05 Jun 2014 06:13:14 GMT

←Older revision		Revision as of 06:13, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3v1x.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate==
		+	<StructureSection load='3v1x' size='340' side='right' caption='[[3v1x]], [[Resolution\|resolution]] 1.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V1X FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0FV:(2Z)-2-FLUORO-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>0FV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v1v\|3v1v]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SC1A4.08, SCBAC12C8.01, SCO7700 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v1x RCSB], [http://www.ebi.ac.uk/pdbsum/3v1x PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of 2-methylisoborneol synthase (MIBS) from Streptomyces coelicolor A3(2) has been determined in complex with substrate analogues geranyl-S-thiolodiphosphate and 2-fluorogeranyl diphosphate at 1.80 and 1.95 A resolution, respectively. This terpenoid cyclase catalyzes the cyclization of the naturally occurring, noncanonical C-methylated isoprenoid substrate, 2-methylgeranyl diphosphate, to form the bicyclic product 2-methylisoborneol, a volatile C(11) homoterpene alcohol with an earthy, musty odor. While MIBS adopts the tertiary structure of a class I terpenoid cyclase, its dimeric quaternary structure differs from that previously observed in dimeric terpenoid cyclases from plants and fungi. The quaternary structure of MIBS is nonetheless similar in some respects to that of dimeric farnesyl diphosphate synthase, which is not a cyclase. The structures of MIBS complexed with substrate analogues provide insights regarding differences in the catalytic mechanism of MIBS and the mechanisms of (+)-bornyl diphosphate synthase and endo-fenchol synthase, plant cyclases that convert geranyl diphosphate into products with closely related bicyclic bornyl skeletons, but distinct structures and stereochemistries.

-	<!--	+	Structure of 2-Methylisoborneol Synthase from Streptomyces coelicolor and Implications for the Cyclization of a Noncanonical C-Methylated Monoterpenoid Substrate.,Koksal M, Chou WK, Cane DE, Christianson DW Biochemistry. 2012 Apr 10;51(14):3011-20. Epub 2012 Mar 28. PMID:22455514<ref>PMID:22455514</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3v1x"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3v1x\| PDB=3v1x \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22455514}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22455514 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22455514}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3v1x]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022455514<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W.]]

OCA at 08:27, 25 April 2012

OCA — Wed, 25 Apr 2012 08:27:46 GMT

←Older revision		Revision as of 08:27, 25 April 2012
Line 1:		Line 1:
-	[[Image:3v1x.~~jpg~~\|left\|200px]]	+	[[Image:3v1x.png\|left\|200px]]

	<!--		<!--

OCA at 07:04, 11 April 2012

OCA — Wed, 11 Apr 2012 07:04:30 GMT

←Older revision		Revision as of 07:04, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3v1x.jpg\|left\|200px]]

-	The ~~entry 3v1x~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3v1x", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3v1x\| PDB=3v1x \| SCENE= }}

-	~~Authors: Koksal, M., Christianson, D.W.~~	+	===Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate===

-	~~Description~~: ~~Crystal structure of 2~~-~~methylisoborneol synthase~~ from Streptomyces coelicolor ~~A3(~~2~~) in complex with Mg2+ and~~ 2-~~fluorogeranyl diphosphate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22455514}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22455514 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22455514}}
		+
		+	==About this Structure==
		+	[[3v1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V1X OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022455514</ref><references group="xtra"/>
		+	[[Category: Streptomyces coelicolor]]
		+	[[Category: Christianson, D W.]]
		+	[[Category: Koksal, M.]]
		+	[[Category: 2-methylisoborneol biosynthesis]]
		+	[[Category: Biosynthesis of 2-methylisoborneol]]
		+	[[Category: Class i terpenoid cyclase fold]]
		+	[[Category: Ddxxxxd motif]]
		+	[[Category: Lyase]]
		+	[[Category: Ndxxsxxxe motif]]

OCA at 05:36, 4 April 2012

OCA — Wed, 04 Apr 2012 05:36:03 GMT

←Older revision		Revision as of 05:36, 4 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v1x is ON HOLD	+	The entry 3v1x is ON HOLD until Paper Publication

	Authors: Koksal, M., Christianson, D.W.		Authors: Koksal, M., Christianson, D.W.

	Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate		Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate

OCA at 06:01, 25 January 2012

OCA — Wed, 25 Jan 2012 06:01:24 GMT

←Older revision		Revision as of 06:01, 25 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v1x is ON HOLD ~~until Paper Publication~~	+	The entry 3v1x is ON HOLD

	Authors: Koksal, M., Christianson, D.W.		Authors: Koksal, M., Christianson, D.W.

	Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate		Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate

OCA at 05:57, 11 January 2012

OCA — Wed, 11 Jan 2012 05:57:43 GMT

←Older revision		Revision as of 05:57, 11 January 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v1x is ON HOLD	+	The entry 3v1x is ON HOLD until Paper Publication

	Authors: Koksal, M., Christianson, D.W.		Authors: Koksal, M., Christianson, D.W.

	Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate		Description: Crystal structure of 2-methylisoborneol synthase from Streptomyces coelicolor A3(2) in complex with Mg2+ and 2-fluorogeranyl diphosphate