3v67 - Revision history

OCA at 08:39, 20 March 2024

OCA — Wed, 20 Mar 2024 08:39:25 GMT

←Older revision		Revision as of 08:39, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3v67' size='340' side='right'caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3v67' size='340' side='right'caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V67 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>VP2859 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~670~~ "~~Oceanomonas parahaemolytica~~" ~~(Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'~~>[~~https://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2~~.7.13~~.3 ~~2.7.13.3] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [https://pdbe.org/3v67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [https://www.ebi.ac.uk/pdbsum/3v67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v67 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [https://pdbe.org/3v67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [https://www.ebi.ac.uk/pdbsum/3v67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v67 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q87KW6_VIBPA Q87KW6_VIBPA]
-	The Cpx two-component system of Gram-negative bacteria senses extracytoplasmic stresses using the histidine kinase CpxA, a membrane-bound sensor, and controls the transcription of the genes involved in stress response by the cytosolic response regulator CpxR, which is activated by the phosphorelay from CpxA. CpxP, a CpxA-associated protein, also plays an important role in the regulation of the Cpx system by inhibiting the autophosphorylation of CpxA. Although the stress signals and physiological roles of the Cpx system have been extensively studied, the lack of structural information has limited the understanding of the detailed mechanism of ligand binding and regulation of CpxA. In this study, we solved the crystal structure of the periplasmic domain of Vibrio parahaemolyticus CpxA (VpCpxA-peri) to a resolution of 2.1 A and investigated its interaction with CpxP. VpCpxA-peri has a globular Per-ARNT-SIM (PAS) domain and a protruded C-terminal tail, which may be required for ligand sensing and CpxP binding, respectively. The direct interaction of the PAS core of VpCpxA-peri with VpCpxP was not detected by NMR, suggesting that the C-terminal tail or other factors, such as the membrane environment, are necessary for the binding of CpxA to CpxP.	+
-		+
-	~~The crystal structure of the periplasmic domain of Vibrio parahaemolyticus CpxA.,Kwon E, Kim DY, Ngo TD, Gross CA, Gross JD, Kim KK Protein Sci. 2012 Sep;21(9)~~:~~1334-43. doi: 10.1002~~/~~pro.2120. PMID:22760860<ref>PMID:22760860<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3v67" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Histidine kinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gross, J D~~]]	+	[[Category: Vibrio parahaemolyticus]]
-	[[Category: ~~Kim, D Y~~]]	+	[[Category: Gross JD]]
-	[[Category: Kim~~, K K~~]]	+	[[Category: Kim DY]]
-	[[Category: ~~Kwon, E~~]]	+	[[Category: Kim KK]]
-	[[Category: ~~Ngo, T D~~]]	+	[[Category: Kwon E]]
-	[[Category: ~~Merohedral twinning]]~~	+	[[Category: Ngo TD]]
-	~~[[Category: Pas fold]]~~	+
-	~~[[Category: Signal sensing]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 08:34, 20 July 2022

OCA — Wed, 20 Jul 2022 08:34:25 GMT

←Older revision		Revision as of 08:34, 20 July 2022
Line 1:		Line 1:

	==Periplasmic domain of Vibrio parahaemolyticus CpxA==		==Periplasmic domain of Vibrio parahaemolyticus CpxA==
-	<StructureSection load='3v67' size='340' side='right' caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3v67' size='340' side='right'caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3V67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V67 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [~~http~~://pdbe.org/3v67 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3v67 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3v67 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [https://pdbe.org/3v67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [https://www.ebi.ac.uk/pdbsum/3v67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v67 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
		+	[[Category: Large Structures]]
	[[Category: Gross, J D]]		[[Category: Gross, J D]]
	[[Category: Kim, D Y]]		[[Category: Kim, D Y]]

OCA at 14:49, 11 August 2016

OCA — Thu, 11 Aug 2016 14:49:57 GMT

←Older revision		Revision as of 14:49, 11 August 2016
Line 1:		Line 1:
		+
	==Periplasmic domain of Vibrio parahaemolyticus CpxA==		==Periplasmic domain of Vibrio parahaemolyticus CpxA==
	<StructureSection load='3v67' size='340' side='right' caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3v67' size='340' side='right' caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_parahaemolyticus Vibrio parahaemolyticus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V67 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 ~~Vibrio parahaemolyticus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [http://www.ebi.ac.uk/pdbsum/3v67 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [http://pdbe.org/3v67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [http://www.ebi.ac.uk/pdbsum/3v67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3v67 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3v67" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
-	[[Category: Vibrio parahaemolyticus]]
	[[Category: Gross, J D]]		[[Category: Gross, J D]]
	[[Category: Kim, D Y]]		[[Category: Kim, D Y]]

OCA at 10:01, 4 January 2015

OCA — Sun, 04 Jan 2015 10:01:22 GMT

←Older revision		Revision as of 10:01, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V67 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 Vibrio parahaemolyticus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 Vibrio parahaemolyticus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [http://www.ebi.ac.uk/pdbsum/3v67 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [http://www.ebi.ac.uk/pdbsum/3v67 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Vibrio parahaemolyticus]]		[[Category: Vibrio parahaemolyticus]]
-	[[Category: Gross, J D.]]	+	[[Category: Gross, J D]]
-	[[Category: Kim, D Y.]]	+	[[Category: Kim, D Y]]
-	[[Category: Kim, K K.]]	+	[[Category: Kim, K K]]
-	[[Category: Kwon, E.]]	+	[[Category: Kwon, E]]
-	[[Category: Ngo, T D.]]	+	[[Category: Ngo, T D]]
	[[Category: Merohedral twinning]]		[[Category: Merohedral twinning]]
	[[Category: Pas fold]]		[[Category: Pas fold]]
	[[Category: Signal sensing]]		[[Category: Signal sensing]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 09:07, 2 July 2014

OCA — Wed, 02 Jul 2014 09:07:46 GMT

←Older revision		Revision as of 09:07, 2 July 2014
Line 1:		Line 1:
-	[[~~Image:~~3v67.~~jpg~~\|~~left\|200px~~]]	+	==Periplasmic domain of Vibrio parahaemolyticus CpxA==
		+	<StructureSection load='3v67' size='340' side='right' caption='[[3v67]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V67 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP2859 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 Vibrio parahaemolyticus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v67 RCSB], [http://www.ebi.ac.uk/pdbsum/3v67 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cpx two-component system of Gram-negative bacteria senses extracytoplasmic stresses using the histidine kinase CpxA, a membrane-bound sensor, and controls the transcription of the genes involved in stress response by the cytosolic response regulator CpxR, which is activated by the phosphorelay from CpxA. CpxP, a CpxA-associated protein, also plays an important role in the regulation of the Cpx system by inhibiting the autophosphorylation of CpxA. Although the stress signals and physiological roles of the Cpx system have been extensively studied, the lack of structural information has limited the understanding of the detailed mechanism of ligand binding and regulation of CpxA. In this study, we solved the crystal structure of the periplasmic domain of Vibrio parahaemolyticus CpxA (VpCpxA-peri) to a resolution of 2.1 A and investigated its interaction with CpxP. VpCpxA-peri has a globular Per-ARNT-SIM (PAS) domain and a protruded C-terminal tail, which may be required for ligand sensing and CpxP binding, respectively. The direct interaction of the PAS core of VpCpxA-peri with VpCpxP was not detected by NMR, suggesting that the C-terminal tail or other factors, such as the membrane environment, are necessary for the binding of CpxA to CpxP.

-	~~{{STRUCTURE_3v67\| PDB=3v67 \| SCENE= }}~~	+	The crystal structure of the periplasmic domain of Vibrio parahaemolyticus CpxA.,Kwon E, Kim DY, Ngo TD, Gross CA, Gross JD, Kim KK Protein Sci. 2012 Sep;21(9):1334-43. doi: 10.1002/pro.2120. PMID:22760860<ref>PMID:22760860</ref>

-	~~===Periplasmic domain~~ of ~~Vibrio parahaemolyticus CpxA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22760860}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3v67]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA].~~	+	</StructureSection>
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Vibrio parahaemolyticus]]		[[Category: Vibrio parahaemolyticus]]

OCA at 20:31, 26 September 2012

OCA — Wed, 26 Sep 2012 20:31:25 GMT

←Older revision		Revision as of 20:31, 26 September 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3v67.jpg\|left\|200px]]

-	~~The entry~~ 3v67 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3v67\| PDB=3v67 \| SCENE= }}

-	~~Authors: Kwon, E., Kim, D.Y., Ngo, T.D., Gross, J.D., Kim, K.K.~~	+	===Periplasmic domain of Vibrio parahaemolyticus CpxA===

-	~~Description~~: ~~Periplasmic domain of~~ Vibrio parahaemolyticus ~~CpxA~~	+	{{ABSTRACT_PUBMED_22760860}}
		+
		+	==About this Structure==
		+	[[3v67]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V67 OCA].
		+	[[Category: Histidine kinase]]
		+	[[Category: Vibrio parahaemolyticus]]
		+	[[Category: Gross, J D.]]
		+	[[Category: Kim, D Y.]]
		+	[[Category: Kim, K K.]]
		+	[[Category: Kwon, E.]]
		+	[[Category: Ngo, T D.]]
		+	[[Category: Merohedral twinning]]
		+	[[Category: Pas fold]]
		+	[[Category: Signal sensing]]
		+	[[Category: Signaling protein]]

OCA at 07:21, 22 February 2012

OCA — Wed, 22 Feb 2012 07:21:12 GMT

←Older revision		Revision as of 07:21, 22 February 2012
Line 3:		Line 3:
	The entry 3v67 is ON HOLD until Paper Publication		The entry 3v67 is ON HOLD until Paper Publication

-	Authors: Kwon, E., Ngo, T.N., Gross, J.D., Kim, K.K.	+	Authors: Kwon, E., Kim, D.Y., Ngo, T.D., Gross, J.D., Kim, K.K.

	Description: Periplasmic domain of Vibrio parahaemolyticus CpxA		Description: Periplasmic domain of Vibrio parahaemolyticus CpxA

OCA at 06:03, 8 February 2012

OCA — Wed, 08 Feb 2012 06:03:17 GMT

←Older revision		Revision as of 06:03, 8 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3v67 is ON HOLD	+	The entry 3v67 is ON HOLD until Paper Publication

-	Authors: Kwon, E., ~~Kim~~, D.~~Y., Gross, C.A~~., Gross, J.D., Kim, K.K.	+	Authors: Kwon, E., Ngo, T.N., Gross, J.D., Kim, K.K.

	Description: Periplasmic domain of Vibrio parahaemolyticus CpxA		Description: Periplasmic domain of Vibrio parahaemolyticus CpxA

OCA at 05:45, 4 January 2012

OCA — Wed, 04 Jan 2012 05:45:50 GMT

←Older revision		Revision as of 05:45, 4 January 2012
Line 3:		Line 3:
	The entry 3v67 is ON HOLD		The entry 3v67 is ON HOLD

-	Authors: Kwon, E., Kim, D. Y., Gross, C. A., Gross, J. D., Kim, K. K.	+	Authors: Kwon, E., Kim, D.Y., Gross, C.A., Gross, J.D., Kim, K.K.

	Description: Periplasmic domain of Vibrio parahaemolyticus CpxA		Description: Periplasmic domain of Vibrio parahaemolyticus CpxA

OCA: Protected "3v67" [edit=sysop:move=sysop]

OCA — Wed, 28 Dec 2011 06:52:10 GMT

Protected "3v67" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 28 December 2011