3vcy - Revision history

OCA at 14:24, 14 March 2024

OCA — Thu, 14 Mar 2024 14:24:17 GMT

←Older revision		Revision as of 14:24, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3vcy' size='340' side='right'caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='3vcy' size='340' side='right'caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Alifm Alifm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_fischeri_MJ11 Aliivibrio fischeri MJ11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.925Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=388396 ALIFM])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [https://pdbe.org/3vcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [https://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [https://pdbe.org/3vcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [https://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~MURA_VIBFM MURA_VIBFM]~~] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/MURA_ALIFM MURA_ALIFM] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.[HAMAP-Rule:MF_00111]
-	~~<div style="background~~-~~color~~:~~#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The development of new antibiotics is necessitated by the rapid development of resistance to current therapies. UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), which catalyzes the first committed step of bacterial peptidoglycan biosynthesis, is a prime candidate for therapeutic intervention. MurA is the target of the antibiotic fosfomycin, a natural product produced by Streptomyces. Despite possessing a high degree of sequence conservation with MurA enzymes from fosfomycin-susceptible organisms, recent microbiological studies suggest that MurA from Vibrio fischeri (VfiMurA) may confer fosfomycin resistance via a mechanism that is not yet understood. The crystal structure of VfiMurA in a ternary complex with the substrate UDP-N-acetylglucosamine (UNAG) and fosfomycin has been solved to a resolution of 1.93 A. Fosfomycin is known to inhibit MurA by covalently binding to a highly conserved cysteine in the active site of the enzyme. A comparison of the title structure with the structure of fosfomycin-susceptible Haemophilus influenzae MurA (PDB entry 2rl2) revealed strikingly similar conformations of the mobile substrate-binding loop and clear electron density for a fosfomycin-cysteine adduct. Based on these results, there are no distinguishing sequence/structural features in VfiMurA that would translate to a diminished sensitivity to fosfomycin. However, VfiMurA is a robust crystallizer and shares high sequence identity with many clinically relevant bacterial pathogens. Thus, it would serve as an ideal system for use in the structure-guided optimization of new antibacterial agents.	+
-		+
-	Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase) from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.,Bensen DC, Rodriguez S, Nix J, Cunningham ML, Tari LW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):382-5. Epub, 2012 Mar 27. PMID:22505403<ref>PMID:22505403</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3vcy" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]		*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alifm~~]]	+	[[Category: Aliivibrio fischeri MJ11]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]~~	+	[[Category: Bensen DC]]
-	[[Category: Bensen~~, D C~~]]	+	[[Category: Cunningham ML]]
-	[[Category: Cunningham~~, M L~~]]	+	[[Category: Nix J]]
-	[[Category: Nix, J]]	+	[[Category: Rodriguez S]]
-	[[Category: Rodriguez, S]]	+	[[Category: Tari LW]]
-	[[Category: Tari~~, L W]]~~	+
-	~~[[Category: Amino sugar and nucleotide sugar metabolism]]~~	+
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Fosfomycin]]~~	+
-	~~[[Category: Mura]]~~	+
-	~~[[Category: Peptidoglycan]]~~	+
-	~~[[Category: Peptidoglycan biosynthesis]]~~	+
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 18:38, 27 July 2022

OCA — Wed, 27 Jul 2022 18:38:52 GMT

←Older revision		Revision as of 18:38, 27 July 2022
Line 1:		Line 1:

	==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==		==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==
-	<StructureSection load='3vcy' size='340' side='right' caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='3vcy' size='340' side='right'caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aiivibrio_fischeri_mj11 Aiivibrio fischeri mj11~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Alifm Alifm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=388396 ~~Aiivibrio fischeri MJ11~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=388396 ALIFM])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [~~http~~://pdbe.org/3vcy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [https://pdbe.org/3vcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [https://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MURA_VIBFM MURA_VIBFM]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity).	+	[[https://www.uniprot.org/uniprot/MURA_VIBFM MURA_VIBFM]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Enoylpyruvate transferase\|Enoylpyruvate transferase]]	+	*[[Enoylpyruvate transferase 3D structures\|Enoylpyruvate transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aiivibrio fischeri mj11~~]]	+	[[Category: Alifm]]
		+	[[Category: Large Structures]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
	[[Category: Bensen, D C]]		[[Category: Bensen, D C]]

OCA at 21:52, 5 August 2016

OCA — Fri, 05 Aug 2016 21:52:33 GMT

←Older revision		Revision as of 21:52, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==		==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==
	<StructureSection load='3vcy' size='340' side='right' caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='3vcy' size='340' side='right' caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aliivibrio_fischeri Aliivibrio~~ fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aiivibrio_fischeri_mj11 Aiivibrio fischeri mj11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~668 Aliivibrio~~ fischeri])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=388396 Aiivibrio fischeri MJ11])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [http://www.ebi.ac.uk/pdbsum/3vcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [http://pdbe.org/3vcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [http://www.ebi.ac.uk/pdbsum/3vcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vcy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aliivibrio~~ fischeri]]	+	[[Category: Aiivibrio fischeri mj11]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
	[[Category: Bensen, D C]]		[[Category: Bensen, D C]]

OCA at 22:13, 25 December 2014

OCA — Thu, 25 Dec 2014 22:13:09 GMT

←Older revision		Revision as of 22:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668 Aliivibrio fischeri])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668 Aliivibrio fischeri])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [http://www.ebi.ac.uk/pdbsum/3vcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [http://www.ebi.ac.uk/pdbsum/3vcy PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MURA_VIBFM MURA_VIBFM]] Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 27:
	[[Category: Aliivibrio fischeri]]		[[Category: Aliivibrio fischeri]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
-	[[Category: Bensen, D C.]]	+	[[Category: Bensen, D C]]
-	[[Category: Cunningham, M L.]]	+	[[Category: Cunningham, M L]]
-	[[Category: Nix, J.]]	+	[[Category: Nix, J]]
-	[[Category: Rodriguez, S.]]	+	[[Category: Rodriguez, S]]
-	[[Category: Tari, L W.]]	+	[[Category: Tari, L W]]
	[[Category: Amino sugar and nucleotide sugar metabolism]]		[[Category: Amino sugar and nucleotide sugar metabolism]]
	[[Category: Cytosol]]		[[Category: Cytosol]]

OCA at 08:15, 5 June 2014

OCA — Thu, 05 Jun 2014 08:15:20 GMT

←Older revision		Revision as of 08:15, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3vcy.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.==
		+	<StructureSection load='3vcy' size='340' side='right' caption='[[3vcy]], [[Resolution\|resolution]] 1.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FFQ:[(1R)-1-HYDROXYPROPYL]PHOSPHONIC+ACID'>FFQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">murA, VFMJ11_0391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668 Aliivibrio fischeri])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vcy RCSB], [http://www.ebi.ac.uk/pdbsum/3vcy PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The development of new antibiotics is necessitated by the rapid development of resistance to current therapies. UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), which catalyzes the first committed step of bacterial peptidoglycan biosynthesis, is a prime candidate for therapeutic intervention. MurA is the target of the antibiotic fosfomycin, a natural product produced by Streptomyces. Despite possessing a high degree of sequence conservation with MurA enzymes from fosfomycin-susceptible organisms, recent microbiological studies suggest that MurA from Vibrio fischeri (VfiMurA) may confer fosfomycin resistance via a mechanism that is not yet understood. The crystal structure of VfiMurA in a ternary complex with the substrate UDP-N-acetylglucosamine (UNAG) and fosfomycin has been solved to a resolution of 1.93 A. Fosfomycin is known to inhibit MurA by covalently binding to a highly conserved cysteine in the active site of the enzyme. A comparison of the title structure with the structure of fosfomycin-susceptible Haemophilus influenzae MurA (PDB entry 2rl2) revealed strikingly similar conformations of the mobile substrate-binding loop and clear electron density for a fosfomycin-cysteine adduct. Based on these results, there are no distinguishing sequence/structural features in VfiMurA that would translate to a diminished sensitivity to fosfomycin. However, VfiMurA is a robust crystallizer and shares high sequence identity with many clinically relevant bacterial pathogens. Thus, it would serve as an ideal system for use in the structure-guided optimization of new antibacterial agents.

-	<!--	+	Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase) from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.,Bensen DC, Rodriguez S, Nix J, Cunningham ML, Tari LW Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):382-5. Epub, 2012 Mar 27. PMID:22505403<ref>PMID:22505403</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3vcy"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3vcy\| PDB=3vcy \| SCENE= }}~~	+

-	~~===Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase)~~, ~~from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and~~ the ~~drug fosfomycin~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Enoylpyruvate transferase\|Enoylpyruvate transferase]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22505403}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22505403 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22505403}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3vcy~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022505403<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aliivibrio fischeri]]		[[Category: Aliivibrio fischeri]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]

OCA at 08:29, 25 April 2012

OCA — Wed, 25 Apr 2012 08:29:13 GMT

←Older revision		Revision as of 08:29, 25 April 2012
Line 1:		Line 1:
-	[[Image:3vcy.~~jpg~~\|left\|200px]]	+	[[Image:3vcy.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.===		===Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22505403}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22505403 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22505403}}

	==About this Structure==		==About this Structure==
	[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA].		[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022505403</ref><references group="xtra"/>
	[[Category: Aliivibrio fischeri]]		[[Category: Aliivibrio fischeri]]
	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]		[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]

OCA at 07:10, 11 April 2012

OCA — Wed, 11 Apr 2012 07:10:07 GMT

←Older revision		Revision as of 07:10, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3vcy.jpg\|left\|200px]]

-	The ~~entry 3vcy~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3vcy", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3vcy\| PDB=3vcy \| SCENE= }}

-	~~Authors: Bensen~~, ~~D.C., Rodriguez, S., Nix, J., Cunningham, M.L., Tari, L.W~~.	+	===Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.===

-	~~Description:~~ Structure ~~of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase),~~ from ~~Vibrio~~ fischeri ~~in complex with substrate~~ UDP-N-acetylglucosamine ~~and the drug fosfomycin~~.	+
		+	==About this Structure==
		+	[[3vcy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aliivibrio_fischeri Aliivibrio fischeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCY OCA].
		+	[[Category: Aliivibrio fischeri]]
		+	[[Category: UDP-N-acetylglucosamine 1-carboxyvinyltransferase]]
		+	[[Category: Bensen, D C.]]
		+	[[Category: Cunningham, M L.]]
		+	[[Category: Nix, J.]]
		+	[[Category: Rodriguez, S.]]
		+	[[Category: Tari, L W.]]
		+	[[Category: Amino sugar and nucleotide sugar metabolism]]
		+	[[Category: Cytosol]]
		+	[[Category: Fosfomycin]]
		+	[[Category: Mura]]
		+	[[Category: Peptidoglycan]]
		+	[[Category: Peptidoglycan biosynthesis]]
		+	[[Category: Transferase-antibiotic complex]]

OCA at 06:53, 1 February 2012

OCA — Wed, 01 Feb 2012 06:53:03 GMT

←Older revision		Revision as of 06:53, 1 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vcy is ON HOLD	+	The entry 3vcy is ON HOLD until Paper Publication

-	Authors: Bensen, D.C., Rodriguez, S., Nix, J., Cunningham, M.L~~., Finn, J~~., Tari, L.W.	+	Authors: Bensen, D.C., Rodriguez, S., Nix, J., Cunningham, M.L., Tari, L.W.

	Description: Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.		Description: Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.

OCA: Protected "3vcy" [edit=sysop:move=sysop]

OCA — Wed, 11 Jan 2012 06:01:52 GMT

Protected "3vcy" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:01, 11 January 2012

OCA: New page: '''Unreleased structure''' The entry 3vcy is ON HOLD Authors: Bensen, D.C., Rodriguez, S., Nix, J., Cunningham, M.L., Finn, J., Tari, L.W. Description: Structure of MurA (UDP-N-acetylg...

OCA — Wed, 11 Jan 2012 06:01:51 GMT

New page: '''Unreleased structure''' The entry 3vcy is ON HOLD Authors: Bensen, D.C., Rodriguez, S., Nix, J., Cunningham, M.L., Finn, J., Tari, L.W. Description: Structure of MurA (UDP-N-acetylg...

New page

'''Unreleased structure'''

The entry 3vcy is ON HOLD

Authors: Bensen, D.C., Rodriguez, S., Nix, J., Cunningham, M.L., Finn, J., Tari, L.W.

Description: Structure of MurA (UDP-N-acetylglucosamine enolpyruvyl transferase), from Vibrio fischeri in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin.