3vp2 - Revision history

OCA at 12:30, 8 November 2023

OCA — Wed, 08 Nov 2023 12:30:49 GMT

←Older revision		Revision as of 12:30, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3vp2' size='340' side='right'caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3vp2' size='340' side='right'caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BP0:5,5~~-~~(SULFANEDIYLDIETHANE-2~~,~~1-DIYL)BIS(1,3,4-THIADIAZOL-~~2~~-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;'>[[3voy\|3voy]]~~, ~~[[3vpz\|3vpz]]~~, ~~[[3vp0\|3vp0]]~~, ~~[[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</~~b></td><td class="sblockDat"~~>~~GLS~~, ~~GLS1, KIAA0838 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [https://pdbe.org/3vp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [https://pdbe.org/3vp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.	+	[https://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutaminase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sivaraman, J]]	+	[[Category: Sivaraman J]]
-	[[Category: Thangavelu, K]]	+	[[Category: Thangavelu K]]
-	~~[[Category: Hydrolase-hydrolase inhibitor complex~~]]	+

OCA at 18:56, 27 July 2022

OCA — Wed, 27 Jul 2022 18:56:35 GMT

←Older revision		Revision as of 18:56, 27 July 2022
Line 1:		Line 1:

	==Crystal structure of human glutaminase in complex with inhibitor 2==		==Crystal structure of human glutaminase in complex with inhibitor 2==
-	<StructureSection load='3vp2' size='340' side='right' caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3vp2' size='340' side='right'caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [~~http~~://pdbe.org/3vp2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vp2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [https://pdbe.org/3vp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.	+	[[https://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Glutaminase\|Glutaminase]]	+	*[[Glutaminase 3D structures\|Glutaminase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Sivaraman, J]]		[[Category: Sivaraman, J]]
	[[Category: Thangavelu, K]]		[[Category: Thangavelu, K]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 20:05, 4 August 2016

OCA — Thu, 04 Aug 2016 20:05:42 GMT

←Older revision		Revision as of 20:05, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of human glutaminase in complex with inhibitor 2==		==Crystal structure of human glutaminase in complex with inhibitor 2==
	<StructureSection load='3vp2' size='340' side='right' caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3vp2' size='340' side='right' caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [http://pdbe.org/3vp2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vp2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Sivaraman, J]]		[[Category: Sivaraman, J]]
	[[Category: Thangavelu, K]]		[[Category: Thangavelu, K]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 19:07, 24 December 2014

OCA — Wed, 24 Dec 2014 19:07:56 GMT

←Older revision		Revision as of 19:07, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp2 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	Structural basis for the allosteric inhibitory mechanism of human kidney-type glutaminase (KGA) and its regulation by Raf-Mek-Erk signaling in cancer cell metabolism.,Thangavelu K, Pan CQ, Karlberg T, Balaji G, Uttamchandani M, Suresh V, Schuler H, Low BC, Sivaraman J Proc Natl Acad Sci U S A. 2012 May 15;109(20):7705-10. Epub 2012 Apr 26. PMID:22538822<ref>PMID:22538822</ref>		Structural basis for the allosteric inhibitory mechanism of human kidney-type glutaminase (KGA) and its regulation by Raf-Mek-Erk signaling in cancer cell metabolism.,Thangavelu K, Pan CQ, Karlberg T, Balaji G, Uttamchandani M, Suresh V, Schuler H, Low BC, Sivaraman J Proc Natl Acad Sci U S A. 2012 May 15;109(20):7705-10. Epub 2012 Apr 26. PMID:22538822<ref>PMID:22538822</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

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	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Sivaraman, J.]]	+	[[Category: Sivaraman, J]]
-	[[Category: Thangavelu, K.]]	+	[[Category: Thangavelu, K]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 07:15, 5 June 2014

OCA — Thu, 05 Jun 2014 07:15:39 GMT

←Older revision		Revision as of 07:15, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3vp2.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of human glutaminase in complex with inhibitor 2==
		+	<StructureSection load='3vp2' size='340' side='right' caption='[[3vp2]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BP0:5,5-(SULFANEDIYLDIETHANE-2,1-DIYL)BIS(1,3,4-THIADIAZOL-2-AMINE)'>BP0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3voy\|3voy]], [[3vpz\|3vpz]], [[3vp0\|3vp0]], [[3vp1\|3vp1]], [[3vp3\|3vp3]], [[3vp4\|3vp4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp2 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Besides thriving on altered glucose metabolism, cancer cells undergo glutaminolysis to meet their energy demands. As the first enzyme in catalyzing glutaminolysis, human kidney-type glutaminase isoform (KGA) is becoming an attractive target for small molecules such as BPTES [bis-2-(5 phenylacetamido-1, 2, 4-thiadiazol-2-yl) ethyl sulfide], although the regulatory mechanism of KGA remains unknown. On the basis of crystal structures, we reveal that BPTES binds to an allosteric pocket at the dimer interface of KGA, triggering a dramatic conformational change of the key loop (Glu312-Pro329) near the catalytic site and rendering it inactive. The binding mode of BPTES on the hydrophobic pocket explains its specificity to KGA. Interestingly, KGA activity in cells is stimulated by EGF, and KGA associates with all three kinase components of the Raf-1/Mek2/Erk signaling module. However, the enhanced activity is abrogated by kinase-dead, dominant negative mutants of Raf-1 (Raf-1-K375M) and Mek2 (Mek2-K101A), protein phosphatase PP2A, and Mek-inhibitor U0126, indicative of phosphorylation-dependent regulation. Furthermore, treating cells that coexpressed Mek2-K101A and KGA with suboptimal level of BPTES leads to synergistic inhibition on cell proliferation. Consequently, mutating the crucial hydrophobic residues at this key loop abrogates KGA activity and cell proliferation, despite the binding of constitutive active Mek2-S222/226D. These studies therefore offer insights into (i) allosteric inhibition of KGA by BPTES, revealing the dynamic nature of KGA's active and inhibitory sites, and (ii) cross-talk and regulation of KGA activities by EGF-mediated Raf-Mek-Erk signaling. These findings will help in the design of better inhibitors and strategies for the treatment of cancers addicted with glutamine metabolism.

-	~~{{STRUCTURE_3vp2\| PDB=3vp2 \| SCENE= }}~~	+	Structural basis for the allosteric inhibitory mechanism of human kidney-type glutaminase (KGA) and its regulation by Raf-Mek-Erk signaling in cancer cell metabolism.,Thangavelu K, Pan CQ, Karlberg T, Balaji G, Uttamchandani M, Suresh V, Schuler H, Low BC, Sivaraman J Proc Natl Acad Sci U S A. 2012 May 15;109(20):7705-10. Epub 2012 Apr 26. PMID:22538822<ref>PMID:22538822</ref>

-	~~===Crystal structure~~ of ~~human glutaminase in complex with inhibitor 2===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22538822}}~~	+	==See Also==
-		+	*[[Glutaminase\|Glutaminase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~3vp2~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022538822<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 04:29, 27 June 2012

OCA — Wed, 27 Jun 2012 04:29:29 GMT

←Older revision		Revision as of 04:29, 27 June 2012
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-	[[Image:3vp2.~~jpg~~\|left\|200px]]	+	[[Image:3vp2.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3vp2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3vp2\| PDB=3vp2 \| SCENE= }}		{{STRUCTURE_3vp2\| PDB=3vp2 \| SCENE= }}

	===Crystal structure of human glutaminase in complex with inhibitor 2===		===Crystal structure of human glutaminase in complex with inhibitor 2===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22538822}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22538822 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22538822}}		{{ABSTRACT_PUBMED_22538822}}

OCA at 06:09, 13 June 2012

OCA — Wed, 13 Jun 2012 06:09:14 GMT

←Older revision		Revision as of 06:09, 13 June 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:3vp2.jpg\|left\|200px]]

-	The ~~entry 3vp2~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3vp2", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3vp2\| PDB=3vp2 \| SCENE= }}

-	~~Authors: Sivaraman, J., Thangavelu, K.~~	+	===Crystal structure of human glutaminase in complex with inhibitor 2===

-	~~Description~~: ~~Crystal~~ structure ~~of human glutaminase in complex~~ with inhibitor 2	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22538822}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22538822 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22538822}}
		+
		+	==About this Structure==
		+	[[3vp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP2 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022538822</ref><references group="xtra"/>
		+	[[Category: Glutaminase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Sivaraman, J.]]
		+	[[Category: Thangavelu, K.]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 18:46, 2 May 2012

OCA — Wed, 02 May 2012 18:46:13 GMT

←Older revision		Revision as of 18:46, 2 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vp2 is ON HOLD	+	The entry 3vp2 is ON HOLD until Paper Publication

	Authors: Sivaraman, J., Thangavelu, K.		Authors: Sivaraman, J., Thangavelu, K.

	Description: Crystal structure of human glutaminase in complex with inhibitor 2		Description: Crystal structure of human glutaminase in complex with inhibitor 2

OCA at 07:14, 28 March 2012

OCA — Wed, 28 Mar 2012 07:14:15 GMT

←Older revision		Revision as of 07:14, 28 March 2012
Line 3:		Line 3:
	The entry 3vp2 is ON HOLD		The entry 3vp2 is ON HOLD

-	Authors: J Sivaraman, K Thangavelu	+	Authors: Sivaraman, J., Thangavelu, K.

	Description: Crystal structure of human glutaminase in complex with inhibitor 2		Description: Crystal structure of human glutaminase in complex with inhibitor 2

OCA: Protected "3vp2" [edit=sysop:move=sysop]

OCA — Wed, 29 Feb 2012 08:17:16 GMT

Protected "3vp2" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:17, 29 February 2012