3vp5 - Revision history

OCA at 08:41, 20 March 2024

OCA — Wed, 20 Mar 2024 08:41:12 GMT

←Older revision		Revision as of 08:41, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3vp5' size='340' side='right'caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3vp5' size='340' side='right'caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lacla Lacla~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~L53789, LL0661, ygfC ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=272623 LACLA])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [https://pdbe.org/3vp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [https://pdbe.org/3vp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp5 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9CHR1_LACLA Q9CHR1_LACLA]
-	Although heme is a crucial element for many biological processes including respiration, heme homeostasis should be regulated strictly due to the cytotoxicity of free heme molecules. Numerous lactic acid bacteria, including Lactococcus lactis, acquire heme molecules exogenously to establish an aerobic respiratory chain. A heme efflux system plays an important role for heme homeostasis to avoid cytotoxicity of acquired free heme, but its regulatory mechanism is not clear. Here, we report that the transcriptional regulator heme-regulated transporter regulator (HrtR) senses and binds a heme molecule as its physiological effector to regulate the expression of the heme-efflux system responsible for heme homeostasis in L. lactis. To elucidate the molecular mechanisms of how HrtR senses a heme molecule and regulates gene expression for the heme efflux system, we determined the crystal structures of the apo-HrtR.DNA complex, apo-HrtR, and holo-HrtR at a resolution of 2.0, 3.1, and 1.9 A, respectively. These structures revealed that HrtR is a member of the TetR family of transcriptional regulators. The residue pair Arg-46 and Tyr-50 plays a crucial role for specific DNA binding through hydrogen bonding and a CH-pi interaction with the DNA bases. HrtR adopts a unique mechanism for its functional regulation upon heme sensing. Heme binding to HrtR causes a coil-to-helix transition of the alpha4 helix in the heme-sensing domain, which triggers a structural change of HrtR, causing it to dissociate from the target DNA for derepression of the genes encoding the heme efflux system. HrtR uses a unique heme-sensing motif with bis-His (His-72 and His-149) ligation to the heme, which is essential for the coil-to-helix transition of the alpha4 helix upon heme sensing.	+
-		+
-	~~Structural Basis for the Transcriptional Regulation of Heme Homeostasis in Lactococcus lactis.,Sawai H, Yamanaka M, Sugimoto H, Shiro Y, Aono S J Biol Chem. 2012 Aug 31;287(36)~~:~~30755-68. Epub 2012 Jul 13. PMID:22798069<ref>PMID:22798069<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3vp5" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lacla~~]]	+	[[Category: Lactococcus lactis subsp. lactis Il1403]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aono, S]]	+	[[Category: Aono S]]
-	[[Category: Sawai, H]]	+	[[Category: Sawai H]]
-	[[Category: Shiro, Y]]	+	[[Category: Shiro Y]]
-	[[Category: Sugimoto, H]]	+	[[Category: Sugimoto H]]
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Sensor protein]]~~	+
-	~~[[Category: Tetr superfamily]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcriptional regulator~~]]	+

OCA at 18:56, 27 July 2022

OCA — Wed, 27 Jul 2022 18:56:37 GMT

←Older revision		Revision as of 18:56, 27 July 2022
Line 1:		Line 1:

	==X-ray Crystal Structure of Wild Type HrtR in the Holo Form==		==X-ray Crystal Structure of Wild Type HrtR in the Holo Form==
-	<StructureSection load='3vp5' size='340' side='right' caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3vp5' size='340' side='right'caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [~~http~~://pdbe.org/3vp5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vp5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [https://pdbe.org/3vp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Lacla]]		[[Category: Lacla]]
		+	[[Category: Large Structures]]
	[[Category: Aono, S]]		[[Category: Aono, S]]
	[[Category: Sawai, H]]		[[Category: Sawai, H]]

OCA at 09:56, 11 August 2016

OCA — Thu, 11 Aug 2016 09:56:39 GMT

←Older revision		Revision as of 09:56, 11 August 2016
Line 1:		Line 1:
		+
	==X-ray Crystal Structure of Wild Type HrtR in the Holo Form==		==X-ray Crystal Structure of Wild Type HrtR in the Holo Form==
	<StructureSection load='3vp5' size='340' side='right' caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3vp5' size='340' side='right' caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactococcus_lactis Lactococcus lactis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1358 Lactococcus lactis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [http://pdbe.org/3vp5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vp5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vp5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lactococcus lactis~~]]	+	[[Category: Lacla]]
	[[Category: Aono, S]]		[[Category: Aono, S]]
	[[Category: Sawai, H]]		[[Category: Sawai, H]]

OCA at 10:22, 4 January 2015

OCA — Sun, 04 Jan 2015 10:22:01 GMT

←Older revision		Revision as of 10:22, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp5 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Lactococcus lactis]]		[[Category: Lactococcus lactis]]
-	[[Category: Aono, S.]]	+	[[Category: Aono, S]]
-	[[Category: Sawai, H.]]	+	[[Category: Sawai, H]]
-	[[Category: Shiro, Y.]]	+	[[Category: Shiro, Y]]
-	[[Category: Sugimoto, H.]]	+	[[Category: Sugimoto, H]]
	[[Category: Heme]]		[[Category: Heme]]
	[[Category: Sensor protein]]		[[Category: Sensor protein]]

OCA at 10:26, 16 June 2014

OCA — Mon, 16 Jun 2014 10:26:58 GMT

←Older revision		Revision as of 10:26, 16 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3vp5.~~png~~\|~~left~~\|~~200px~~]]	+	==X-ray Crystal Structure of Wild Type HrtR in the Holo Form==
		+	<StructureSection load='3vp5' size='340' side='right' caption='[[3vp5]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VP5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L53789, LL0661, ygfC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vp5 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although heme is a crucial element for many biological processes including respiration, heme homeostasis should be regulated strictly due to the cytotoxicity of free heme molecules. Numerous lactic acid bacteria, including Lactococcus lactis, acquire heme molecules exogenously to establish an aerobic respiratory chain. A heme efflux system plays an important role for heme homeostasis to avoid cytotoxicity of acquired free heme, but its regulatory mechanism is not clear. Here, we report that the transcriptional regulator heme-regulated transporter regulator (HrtR) senses and binds a heme molecule as its physiological effector to regulate the expression of the heme-efflux system responsible for heme homeostasis in L. lactis. To elucidate the molecular mechanisms of how HrtR senses a heme molecule and regulates gene expression for the heme efflux system, we determined the crystal structures of the apo-HrtR.DNA complex, apo-HrtR, and holo-HrtR at a resolution of 2.0, 3.1, and 1.9 A, respectively. These structures revealed that HrtR is a member of the TetR family of transcriptional regulators. The residue pair Arg-46 and Tyr-50 plays a crucial role for specific DNA binding through hydrogen bonding and a CH-pi interaction with the DNA bases. HrtR adopts a unique mechanism for its functional regulation upon heme sensing. Heme binding to HrtR causes a coil-to-helix transition of the alpha4 helix in the heme-sensing domain, which triggers a structural change of HrtR, causing it to dissociate from the target DNA for derepression of the genes encoding the heme efflux system. HrtR uses a unique heme-sensing motif with bis-His (His-72 and His-149) ligation to the heme, which is essential for the coil-to-helix transition of the alpha4 helix upon heme sensing.

-	~~{{STRUCTURE_3vp5\| PDB=3vp5 \| SCENE= }}~~	+	Structural Basis for the Transcriptional Regulation of Heme Homeostasis in Lactococcus lactis.,Sawai H, Yamanaka M, Sugimoto H, Shiro Y, Aono S J Biol Chem. 2012 Aug 31;287(36):30755-68. Epub 2012 Jul 13. PMID:22798069<ref>PMID:22798069</ref>

-	~~===X-ray Crystal Structure~~ of ~~Wild Type HrtR in~~ the ~~Holo Form===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22798069}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3vp5]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA].~~	+	</StructureSection>
	[[Category: Lactococcus lactis]]		[[Category: Lactococcus lactis]]
	[[Category: Aono, S.]]		[[Category: Aono, S.]]

OCA at 05:15, 12 September 2012

OCA — Wed, 12 Sep 2012 05:15:28 GMT

←Older revision		Revision as of 05:15, 12 September 2012
Line 1:		Line 1:
-	[[Image:3vp5.~~jpg~~\|left\|200px]]	+	[[Image:3vp5.png\|left\|200px]]

	{{STRUCTURE_3vp5\| PDB=3vp5 \| SCENE= }}		{{STRUCTURE_3vp5\| PDB=3vp5 \| SCENE= }}
Line 5:		Line 5:
	===X-ray Crystal Structure of Wild Type HrtR in the Holo Form===		===X-ray Crystal Structure of Wild Type HrtR in the Holo Form===

		+	{{ABSTRACT_PUBMED_22798069}}

	==About this Structure==		==About this Structure==

OCA at 07:17, 25 July 2012

OCA — Wed, 25 Jul 2012 07:17:00 GMT

←Older revision		Revision as of 07:17, 25 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3vp5.jpg\|left\|200px]]

-	~~The entry~~ 3vp5 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_3vp5\| PDB=3vp5 \| SCENE= }}

-	~~Authors: Sawai, H., Sugimoto, H., Shiro, Y., Aono, S.~~	+	===X-ray Crystal Structure of Wild Type HrtR in the Holo Form===

-	~~Description~~: X-~~ray Crystal Structure of Wild Type HrtR in the Holo Form~~	+
		+	==About this Structure==
		+	[[3vp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VP5 OCA].
		+	[[Category: Lactococcus lactis]]
		+	[[Category: Aono, S.]]
		+	[[Category: Sawai, H.]]
		+	[[Category: Shiro, Y.]]
		+	[[Category: Sugimoto, H.]]
		+	[[Category: Heme]]
		+	[[Category: Sensor protein]]
		+	[[Category: Tetr superfamily]]
		+	[[Category: Transcription]]
		+	[[Category: Transcriptional regulator]]

OCA at 06:05, 14 March 2012

OCA — Wed, 14 Mar 2012 06:05:19 GMT

←Older revision		Revision as of 06:05, 14 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vp5 is ON HOLD	+	The entry 3vp5 is ON HOLD until Paper Publication

	Authors: Sawai, H., Sugimoto, H., Shiro, Y., Aono, S.		Authors: Sawai, H., Sugimoto, H., Shiro, Y., Aono, S.

	Description: X-ray Crystal Structure of Wild Type HrtR in the Holo Form		Description: X-ray Crystal Structure of Wild Type HrtR in the Holo Form

OCA at 05:20, 8 March 2012

OCA — Thu, 08 Mar 2012 05:20:11 GMT

←Older revision		Revision as of 05:20, 8 March 2012
Line 3:		Line 3:
	The entry 3vp5 is ON HOLD		The entry 3vp5 is ON HOLD

-	Authors: Sawai, ~~Hitomi~~, Sugimoto, ~~Hiroshi~~, Shiro, ~~Yoshitsugu~~, Aono, ~~Shigetoshi~~	+	Authors: Sawai, H., Sugimoto, H., Shiro, Y., Aono, S.

-	Description: X-ray Crystal Structure of Wild Type HrtR in the Holo Form.	+	Description: X-ray Crystal Structure of Wild Type HrtR in the Holo Form

OCA: Protected "3vp5" [edit=sysop:move=sysop]

OCA — Wed, 29 Feb 2012 08:17:21 GMT

Protected "3vp5" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:17, 29 February 2012