3vu9 - Revision history

OCA at 08:42, 20 March 2024

OCA — Wed, 20 Mar 2024 08:42:06 GMT

←Older revision		Revision as of 08:42, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3vu9' size='340' side='right'caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vu9' size='340' side='right'caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PSY3, YLR376C, L8039.17 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=559292 Baker~~'~~s yeast]), CSM2, YIL132C ([https:~~/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [https://pdbe.org/3vu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [https://www.ebi.ac.uk/pdbsum/3vu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [https://pdbe.org/3vu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [https://www.ebi.ac.uk/pdbsum/3vu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[https://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>	+	[https://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	During homologous recombination, eukaryotic RecA homologue Rad51 assembles into a nucleoprotein filament on single-stranded DNA to catalyse homologous pairing and DNA-strand exchange with a homologous template. Rad51 nucleoprotein filaments are highly dynamic and regulated via the coordinated actions of various accessory proteins including Rad51 mediators. Here, we identify a new Rad51 mediator complex. The PCSS complex, comprising budding yeast Psy3, Csm2, Shu1 and Shu2 proteins, binds to recombination sites and is required for Rad51 assembly and function during meiosis. Within the hetero-tetramer, Psy3-Csm2 constitutes a core sub-complex with DNA-binding activity. In vitro, purified Psy3-Csm2 stabilizes the Rad51-single-stranded DNA complex independently of nucleotide cofactor. The mechanism of Rad51 stabilization is inferred by our high-resolution crystal structure, which reveals Psy3-Csm2 to be a structural mimic of the Rad51-dimer, a fundamental unit of the Rad51-filament. Together, these results reveal a novel molecular mechanism for this class of Rad51-mediators, which includes the human Rad51 paralogues.	+
-		+
-	A new protein complex promoting the assembly of Rad51 filaments.,Sasanuma H, Tawaramoto MS, Lao JP, Hosaka H, Sanda E, Suzuki M, Yamashita E, Hunter N, Shinohara M, Nakagawa A, Shinohara A Nat Commun. 2013;4:1676. doi: 10.1038/ncomms2678. PMID:23575680<ref>PMID:23575680</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3vu9" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hosaka, H]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Hunter, N]]	+	[[Category: Hosaka H]]
-	[[Category: Lao~~, J P~~]]	+	[[Category: Hunter N]]
-	[[Category: Nakagawa, A]]	+	[[Category: Lao JP]]
-	[[Category: Sanda, E]]	+	[[Category: Nakagawa A]]
-	[[Category: Sasanuma, H]]	+	[[Category: Sanda E]]
-	[[Category: Shinohara, A]]	+	[[Category: Sasanuma H]]
-	[[Category: Shinohara, M]]	+	[[Category: Shinohara A]]
-	[[Category: Suzuki, M]]	+	[[Category: Shinohara M]]
-	[[Category: Tawaramoto, M]]	+	[[Category: Suzuki M]]
-	[[Category: Yamashita, E]]	+	[[Category: Tawaramoto M]]
-	~~[[Category: Dna binding protein]]~~	+	[[Category: Yamashita E]]
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Meiosis]]~~	+
-	~~[[Category: Recombination~~]]	+

OCA at 19:04, 27 July 2022

OCA — Wed, 27 Jul 2022 19:04:26 GMT

←Older revision		Revision as of 19:04, 27 July 2022
Line 1:		Line 1:

	==Crystal Structure of Psy3-Csm2 complex==		==Crystal Structure of Psy3-Csm2 complex==
-	<StructureSection load='3vu9' size='340' side='right' caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3vu9' size='340' side='right'caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PSY3, YLR376C, L8039.17 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), CSM2, YIL132C ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PSY3, YLR376C, L8039.17 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), CSM2, YIL132C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [~~http~~://pdbe.org/3vu9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vu9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [https://pdbe.org/3vu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [https://www.ebi.ac.uk/pdbsum/3vu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[~~http~~://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>	+	[[https://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[https://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Hosaka, H]]		[[Category: Hosaka, H]]
	[[Category: Hunter, N]]		[[Category: Hunter, N]]

OCA at 13:02, 5 August 2016

OCA — Fri, 05 Aug 2016 13:02:03 GMT

←Older revision		Revision as of 13:02, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Psy3-Csm2 complex==		==Crystal Structure of Psy3-Csm2 complex==
	<StructureSection load='3vu9' size='340' side='right' caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vu9' size='340' side='right' caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PSY3, YLR376C, L8039.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~]), CSM2, YIL132C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PSY3, YLR376C, L8039.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), CSM2, YIL132C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [http://pdbe.org/3vu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vu9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Hosaka, H]]		[[Category: Hosaka, H]]
	[[Category: Hunter, N]]		[[Category: Hunter, N]]

OCA at 02:41, 25 December 2014

OCA — Thu, 25 Dec 2014 02:41:06 GMT

←Older revision		Revision as of 02:41, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[http://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:36, 21 December 2014

OCA — Sun, 21 Dec 2014 06:36:03 GMT

←Older revision		Revision as of 06:36, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vu9~~\| ~~PDB~~=3vu9 \| ~~SCENE~~= }}	+	==Crystal Structure of Psy3-Csm2 complex==
-	===~~Crystal Structure of Psy3~~-~~Csm2 complex~~===	+	<StructureSection load='3vu9' size='340' side='right' caption='[[3vu9]], [[Resolution\|resolution]] 1.75Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23575680}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vu9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VU9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PSY3, YLR376C, L8039.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), CSM2, YIL132C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vu9 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu9 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During homologous recombination, eukaryotic RecA homologue Rad51 assembles into a nucleoprotein filament on single-stranded DNA to catalyse homologous pairing and DNA-strand exchange with a homologous template. Rad51 nucleoprotein filaments are highly dynamic and regulated via the coordinated actions of various accessory proteins including Rad51 mediators. Here, we identify a new Rad51 mediator complex. The PCSS complex, comprising budding yeast Psy3, Csm2, Shu1 and Shu2 proteins, binds to recombination sites and is required for Rad51 assembly and function during meiosis. Within the hetero-tetramer, Psy3-Csm2 constitutes a core sub-complex with DNA-binding activity. In vitro, purified Psy3-Csm2 stabilizes the Rad51-single-stranded DNA complex independently of nucleotide cofactor. The mechanism of Rad51 stabilization is inferred by our high-resolution crystal structure, which reveals Psy3-Csm2 to be a structural mimic of the Rad51-dimer, a fundamental unit of the Rad51-filament. Together, these results reveal a novel molecular mechanism for this class of Rad51-mediators, which includes the human Rad51 paralogues.

-	~~==Function==~~	+	A new protein complex promoting the assembly of Rad51 filaments.,Sasanuma H, Tawaramoto MS, Lao JP, Hosaka H, Sanda E, Suzuki M, Yamashita E, Hunter N, Shinohara M, Nakagawa A, Shinohara A Nat Commun. 2013;4:1676. doi: 10.1038/ncomms2678. PMID:23575680<ref>PMID:23575680</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS)~~, ~~cisplatin and oxaliplatin~~, ~~but not to mitomycin C~~. ~~Plays a role in protection against mutation accumulation~~. ~~May be a component of the recombination-repair pathway.<ref>PMID~~:~~12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[http://www~~.~~uniprot.org/uniprot~~/~~CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis~~. ~~Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>~~PMID:~~11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref>~~ <ref>PMID:~~19496932~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3vu9]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023575680</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Hosaka, H.]]	+	[[Category: Hosaka, H]]
-	[[Category: Hunter, N.]]	+	[[Category: Hunter, N]]
-	[[Category: Lao, J P.]]	+	[[Category: Lao, J P]]
-	[[Category: Nakagawa, A.]]	+	[[Category: Nakagawa, A]]
-	[[Category: Sanda, E.]]	+	[[Category: Sanda, E]]
-	[[Category: Sasanuma, H.]]	+	[[Category: Sasanuma, H]]
-	[[Category: Shinohara, A.]]	+	[[Category: Shinohara, A]]
-	[[Category: Shinohara, M.]]	+	[[Category: Shinohara, M]]
-	[[Category: Suzuki, M.]]	+	[[Category: Suzuki, M]]
-	[[Category: Tawaramoto, M.]]	+	[[Category: Tawaramoto, M]]
-	[[Category: Yamashita, E.]]	+	[[Category: Yamashita, E]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Dna repair]]		[[Category: Dna repair]]
	[[Category: Meiosis]]		[[Category: Meiosis]]
	[[Category: Recombination]]		[[Category: Recombination]]

OCA at 07:10, 30 June 2013

OCA — Sun, 30 Jun 2013 07:10:36 GMT

←Older revision		Revision as of 07:10, 30 June 2013
Line 1:		Line 1:
	{{STRUCTURE_3vu9\| PDB=3vu9 \| SCENE= }}		{{STRUCTURE_3vu9\| PDB=3vu9 \| SCENE= }}
	===Crystal Structure of Psy3-Csm2 complex===		===Crystal Structure of Psy3-Csm2 complex===
		+	{{ABSTRACT_PUBMED_23575680}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:023575680</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
	[[Category: Hosaka, H.]]		[[Category: Hosaka, H.]]

OCA at 22:37, 10 April 2013

OCA — Wed, 10 Apr 2013 22:37:41 GMT

←Older revision		Revision as of 22:37, 10 April 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3vu9\| PDB=3vu9 \| SCENE= }}
		+	===Crystal Structure of Psy3-Csm2 complex===

-	~~The entry 3vu9 is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref> [[http://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>

-	~~Authors~~: ~~Tawaramoto, M~~.~~, Sasanuma, H~~.~~, Hosaka, H~~.~~, Lao, J.P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N~~.~~, Shinohara, M~~.~~, Nakagawa, A~~.~~, Shinohara, A~~.	+	==About this Structure==
		+	[[3vu9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU9 OCA].

-	~~Description~~: ~~Crystal Structure of Psy3-Csm2 complex~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Hosaka, H.]]
		+	[[Category: Hunter, N.]]
		+	[[Category: Lao, J P.]]
		+	[[Category: Nakagawa, A.]]
		+	[[Category: Sanda, E.]]
		+	[[Category: Sasanuma, H.]]
		+	[[Category: Shinohara, A.]]
		+	[[Category: Shinohara, M.]]
		+	[[Category: Suzuki, M.]]
		+	[[Category: Tawaramoto, M.]]
		+	[[Category: Yamashita, E.]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna repair]]
		+	[[Category: Meiosis]]
		+	[[Category: Recombination]]

OCA at 08:00, 27 March 2013

OCA — Wed, 27 Mar 2013 08:00:15 GMT

←Older revision		Revision as of 08:00, 27 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vu9 is ON HOLD	+	The entry 3vu9 is ON HOLD until Paper Publication

	Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J.P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.		Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J.P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.

	Description: Crystal Structure of Psy3-Csm2 complex		Description: Crystal Structure of Psy3-Csm2 complex

OCA at 05:14, 1 August 2012

OCA — Wed, 01 Aug 2012 05:14:51 GMT

←Older revision		Revision as of 05:14, 1 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vu9 is ON HOLD ~~until sometime in the future~~	+	The entry 3vu9 is ON HOLD

-	Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J. P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.	+	Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J.P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.

	Description: Crystal Structure of Psy3-Csm2 complex		Description: Crystal Structure of Psy3-Csm2 complex

OCA at 06:35, 25 July 2012

OCA — Wed, 25 Jul 2012 06:35:47 GMT

←Older revision		Revision as of 06:35, 25 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vu9 is ON HOLD	+	The entry 3vu9 is ON HOLD until sometime in the future

	Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J. P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.		Authors: Tawaramoto, M., Sasanuma, H., Hosaka, H., Lao, J. P., Sanda, E., Suzuki, M., Yamashita, E., Hunter, N., Shinohara, M., Nakagawa, A., Shinohara, A.

	Description: Crystal Structure of Psy3-Csm2 complex		Description: Crystal Structure of Psy3-Csm2 complex