3vua - Revision history

OCA at 12:38, 8 November 2023

OCA — Wed, 08 Nov 2023 12:38:01 GMT

←Older revision		Revision as of 12:38, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3vua' size='340' side='right'caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3vua' size='340' side='right'caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VUA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [https://pdbe.org/3vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [https://www.ebi.ac.uk/pdbsum/3vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vua ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [https://pdbe.org/3vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [https://www.ebi.ac.uk/pdbsum/3vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vua ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).	+	[https://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Antibiotic resistance is increasingly seen as a serious problem that threatens public health and erodes our capacity to effectively combat disease. So-called non-iron metalloporhyrins have shown promising antibacterial properties against a number of pathogenic bacteria including Staphylococcus aureus. However, little is known about the molecular mechanism(s) of action of these compounds and in particular how they reach the interior of the bacterial cells. A popular hypothesis indicates that non-iron metalloporphyrins infiltrate into bacterial cells like a "Trojan horse" using heme transport systems. Iron-regulated surface determinant (Isd) is the best characterized heme transport system of S. aureus. Herein we studied the molecular mechanism by which the extracellular heme-receptor IsdH-NEAT3 of Isd recognizes antimicrobial metalloporphyrins. We found that potent antibacterial porphyrins Ga(III)-protoporphyrin IX (PPIX) and Mn(III)-PPIX closely mimicked the properties of the natural ligand heme, namely (i) stable binding to IsdH-NEAT3 with comparable affinities for the receptor, (ii) nearly undistinghuishable three-dimensional structure when complexed with IsdH-NEAT3, and (iii) similar transfer properties to a second receptor IsdA. On the contrary, weaker antibacterial porphyrins Mg(II)-PPIX, Zn(II)-PPIX, and Cu(II)-PPIX were not captured effectively by IsdH-NEAT3 under our experimental conditions and displayed lower affinities. Moreover, reduction of Fe(III)-PPIX to Fe(II)-PPIX with dithionite abrogated stable binding to receptor. These data revealed a clear connection between oxidation state of metal and effective attachment to IsdH-NEAT3. Also, the strong correlation between binding affinity and reported antimicrobial potency suggested that the Isd system may be used by these antibacterial compounds to gain access to the interior of the cells. We hope these results will increase our understanding of Isd system of S. aureus and highlight its biomedical potential to deliver new and more efficient antibacterial treatments.	+
-		+
-	Molecular basis of recognition of antibacterial porphyrins by heme-transporter IsdH-NEAT3 of Staphylococcus aureus.,Moriwaki Y, Caaveiro JM, Tanaka Y, Tsutsumi H, Hamachi I, Tsumoto K Biochemistry. 2011 Aug 30;50(34):7311-20. Epub 2011 Aug 4. PMID:21797259<ref>PMID:21797259</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Haptoglobin receptor\|Haptoglobin receptor]]		*[[Haptoglobin receptor\|Haptoglobin receptor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staam~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: Caaveiro~~, J M.M~~]]	+	[[Category: Caaveiro JMM]]
-	[[Category: Moriwaki, Y]]	+	[[Category: Moriwaki Y]]
-	[[Category: Tsumoto, K]]	+	[[Category: Tsumoto K]]
-	[[Category: Vu~~, N T]]~~	+	[[Category: Vu NT]]
-	~~[[Category: Apo form]]~~	+
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: Heme binding]]~~	+
-	~~[[Category: Heme transport]]~~	+
-	~~[[Category: Heme-binding protein]]~~	+
-	~~[[Category: Neat domain~~]]	+

OCA at 19:04, 27 July 2022

OCA — Wed, 27 Jul 2022 19:04:29 GMT

←Older revision		Revision as of 19:04, 27 July 2022
Line 1:		Line 1:

	==Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A==		==Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A==
-	<StructureSection load='3vua' size='340' side='right' caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3vua' size='340' side='right'caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VUA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [~~http~~://pdbe.org/3vua PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vua PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vua ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [https://pdbe.org/3vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [https://www.ebi.ac.uk/pdbsum/3vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vua ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).	+	[[https://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Haptoglobin receptor\|Haptoglobin receptor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staam]]		[[Category: Staam]]
	[[Category: Caaveiro, J M.M]]		[[Category: Caaveiro, J M.M]]

OCA at 07:58, 22 November 2017

OCA — Wed, 22 Nov 2017 07:58:03 GMT

←Older revision		Revision as of 07:58, 22 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Haptoglobin receptor\|Haptoglobin receptor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:11, 5 August 2016

OCA — Fri, 05 Aug 2016 04:11:54 GMT

←Older revision		Revision as of 04:11, 5 August 2016
Line 1:		Line 1:
		+
	==Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A==		==Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A==
	<StructureSection load='3vua' size='340' side='right' caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3vua' size='340' side='right' caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 ~~Staphylococcus aureus subsp. aureus Mu50~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [http://www.ebi.ac.uk/pdbsum/3vua PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [http://pdbe.org/3vua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [http://www.ebi.ac.uk/pdbsum/3vua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vua ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vua" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Haptoglobin receptor\|Haptoglobin receptor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus mu50~~]]	+	[[Category: Staam]]
	[[Category: Caaveiro, J M.M]]		[[Category: Caaveiro, J M.M]]
	[[Category: Moriwaki, Y]]		[[Category: Moriwaki, Y]]

OCA at 15:43, 25 December 2014

OCA — Thu, 25 Dec 2014 15:43:47 GMT

←Older revision		Revision as of 15:43, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [http://www.ebi.ac.uk/pdbsum/3vua PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [http://www.ebi.ac.uk/pdbsum/3vua PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:50, 21 December 2014

OCA — Sun, 21 Dec 2014 06:50:06 GMT

←Older revision		Revision as of 06:50, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vua\| PDB=3vua \| SCENE= }}~~	+	==Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A==
-	===Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A===	+	<StructureSection load='3vua' size='340' side='right' caption='[[3vua]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21797259}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vua]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VUA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3quh\|3quh]], [[3qug\|3qug]], [[2z6f\|2z6f]], [[2e7d\|2e7d]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">harA, isdH, IsdH-NEAT3, sasI, SAV1731 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vua RCSB], [http://www.ebi.ac.uk/pdbsum/3vua PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibiotic resistance is increasingly seen as a serious problem that threatens public health and erodes our capacity to effectively combat disease. So-called non-iron metalloporhyrins have shown promising antibacterial properties against a number of pathogenic bacteria including Staphylococcus aureus. However, little is known about the molecular mechanism(s) of action of these compounds and in particular how they reach the interior of the bacterial cells. A popular hypothesis indicates that non-iron metalloporphyrins infiltrate into bacterial cells like a "Trojan horse" using heme transport systems. Iron-regulated surface determinant (Isd) is the best characterized heme transport system of S. aureus. Herein we studied the molecular mechanism by which the extracellular heme-receptor IsdH-NEAT3 of Isd recognizes antimicrobial metalloporphyrins. We found that potent antibacterial porphyrins Ga(III)-protoporphyrin IX (PPIX) and Mn(III)-PPIX closely mimicked the properties of the natural ligand heme, namely (i) stable binding to IsdH-NEAT3 with comparable affinities for the receptor, (ii) nearly undistinghuishable three-dimensional structure when complexed with IsdH-NEAT3, and (iii) similar transfer properties to a second receptor IsdA. On the contrary, weaker antibacterial porphyrins Mg(II)-PPIX, Zn(II)-PPIX, and Cu(II)-PPIX were not captured effectively by IsdH-NEAT3 under our experimental conditions and displayed lower affinities. Moreover, reduction of Fe(III)-PPIX to Fe(II)-PPIX with dithionite abrogated stable binding to receptor. These data revealed a clear connection between oxidation state of metal and effective attachment to IsdH-NEAT3. Also, the strong correlation between binding affinity and reported antimicrobial potency suggested that the Isd system may be used by these antibacterial compounds to gain access to the interior of the cells. We hope these results will increase our understanding of Isd system of S. aureus and highlight its biomedical potential to deliver new and more efficient antibacterial treatments.

-	~~==Function==~~	+	Molecular basis of recognition of antibacterial porphyrins by heme-transporter IsdH-NEAT3 of Staphylococcus aureus.,Moriwaki Y, Caaveiro JM, Tanaka Y, Tsutsumi H, Hamachi I, Tsumoto K Biochemistry. 2011 Aug 30;50(34):7311-20. Epub 2011 Aug 4. PMID:21797259<ref>PMID:21797259</ref>
-	~~[[http://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin~~-~~hemoglobin complexes~~, ~~haptoglobin and hemoglobin~~. ~~Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone~~ (~~By similarity~~).	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3vua]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus_subsp~~.~~_aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021797259</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus mu50]]		[[Category: Staphylococcus aureus subsp. aureus mu50]]
-	[[Category: Caaveiro, J M.M.]]	+	[[Category: Caaveiro, J M.M]]
-	[[Category: Moriwaki, Y.]]	+	[[Category: Moriwaki, Y]]
-	[[Category: Tsumoto, K.]]	+	[[Category: Tsumoto, K]]
-	[[Category: Vu, N T.]]	+	[[Category: Vu, N T]]
	[[Category: Apo form]]		[[Category: Apo form]]
	[[Category: Cell wall]]		[[Category: Cell wall]]

OCA at 10:18, 30 June 2013

OCA — Sun, 30 Jun 2013 10:18:57 GMT

←Older revision		Revision as of 10:18, 30 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3vua\| PDB=3vua \| SCENE= }}
		+	===Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A===
		+	{{ABSTRACT_PUBMED_21797259}}

-	~~The entry 3vua is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ISDH_STAAM ISDH_STAAM]] Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (By similarity).

-	~~Authors~~: ~~Vu, N~~.T.~~, Caaveiro, J~~.M.M.~~, Moriwaki, Y~~.~~, Tsumoto, K~~.	+	==About this Structure==
		+	[[3vua]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VUA OCA].

-	~~Description~~: ~~Apo IsdH-NEAT3 in space~~ group ~~P3121 at a resolution of 1~~.~~85 A~~	+	==Reference==
		+	<ref group="xtra">PMID:021797259</ref><references group="xtra"/><references/>
		+	[[Category: Staphylococcus aureus subsp. aureus mu50]]
		+	[[Category: Caaveiro, J M.M.]]
		+	[[Category: Moriwaki, Y.]]
		+	[[Category: Tsumoto, K.]]
		+	[[Category: Vu, N T.]]
		+	[[Category: Apo form]]
		+	[[Category: Cell wall]]
		+	[[Category: Heme binding]]
		+	[[Category: Heme transport]]
		+	[[Category: Heme-binding protein]]
		+	[[Category: Neat domain]]

OCA at 06:35, 25 July 2012

OCA — Wed, 25 Jul 2012 06:35:49 GMT

←Older revision		Revision as of 06:35, 25 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vua is ON HOLD	+	The entry 3vua is ON HOLD until Paper Publication

	Authors: Vu, N.T., Caaveiro, J.M.M., Moriwaki, Y., Tsumoto, K.		Authors: Vu, N.T., Caaveiro, J.M.M., Moriwaki, Y., Tsumoto, K.

	Description: Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A		Description: Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A

OCA: Protected "3vua" [edit=sysop:move=sysop]

OCA — Wed, 04 Jul 2012 07:05:44 GMT

Protected "3vua" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:05, 4 July 2012

OCA: New page: '''Unreleased structure''' The entry 3vua is ON HOLD Authors: Vu, N.T., Caaveiro, J.M.M., Moriwaki, Y., Tsumoto, K. Description: Apo IsdH-NEAT3 in space group P3121 at a resolution of ...

OCA — Wed, 04 Jul 2012 07:05:43 GMT

New page: '''Unreleased structure''' The entry 3vua is ON HOLD Authors: Vu, N.T., Caaveiro, J.M.M., Moriwaki, Y., Tsumoto, K. Description: Apo IsdH-NEAT3 in space group P3121 at a resolution of ...

New page

'''Unreleased structure'''

The entry 3vua is ON HOLD

Authors: Vu, N.T., Caaveiro, J.M.M., Moriwaki, Y., Tsumoto, K.

Description: Apo IsdH-NEAT3 in space group P3121 at a resolution of 1.85 A