3vwa - Revision history

OCA at 19:07, 27 July 2022

OCA — Wed, 27 Jul 2022 19:07:29 GMT

←Older revision		Revision as of 19:07, 27 July 2022
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	==Crystal structure of Cex1p==		==Crystal structure of Cex1p==
-	<StructureSection load='3vwa' size='340' side='right' caption='[[3vwa]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3vwa' size='340' side='right'caption='[[3vwa]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VWA FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [~~http~~://pdbe.org/3vwa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vwa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vwa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [https://pdbe.org/3vwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [https://www.ebi.ac.uk/pdbsum/3vwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vwa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref>	+	[[https://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]
	[[Category: Nozawa, K]]		[[Category: Nozawa, K]]

OCA at 21:12, 5 August 2016

OCA — Fri, 05 Aug 2016 21:12:44 GMT

←Older revision		Revision as of 21:12, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Cex1p==		==Crystal structure of Cex1p==
	<StructureSection load='3vwa' size='340' side='right' caption='[[3vwa]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3vwa' size='340' side='right' caption='[[3vwa]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VWA FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://pdbe.org/3vwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vwa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vwa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]
	[[Category: Nozawa, K]]		[[Category: Nozawa, K]]

OCA at 21:56, 25 December 2014

OCA — Thu, 25 Dec 2014 21:56:18 GMT

←Older revision		Revision as of 21:56, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:44, 21 December 2014

OCA — Sun, 21 Dec 2014 06:44:18 GMT

←Older revision		Revision as of 06:44, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vwa\| PDB~~=3vwa \| ~~SCENE~~= }}	+	==Crystal structure of Cex1p==
-	===~~Crystal~~ structure ~~of Cex1p~~===	+	<StructureSection load='3vwa' size='340' side='right' caption='[[3vwa]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23396276}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VWA FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In all eukaryotes, transcribed precursor tRNAs are maturated by processing and modification processes in nucleus and are transported to the cytoplasm. The cytoplasmic export protein (Cex1p) captures mature tRNAs from the nuclear export receptor (Los1p) on the cytoplasmic side of the nuclear pore complex, and it delivers them to eukaryotic elongation factor 1alpha. This conserved Cex1p function is essential for the quality control of mature tRNAs to ensure accurate translation. However, the structural basis of how Cex1p recognizes tRNAs and shuttles them to the translational apparatus remains unclear. Here, we solved the 2.2 A resolution crystal structure of Saccharomyces cerevisiae Cex1p with C-terminal 197 disordered residues truncated. Cex1p adopts an elongated architecture, consisting of N-terminal kinase-like and a C-terminal alpha-helical HEAT repeat domains. Structure-based biochemical analyses suggested that Cex1p binds tRNAs on its inner side, using the positively charged HEAT repeat surface and the C-terminal disordered region. The N-terminal kinase-like domain acts as a scaffold to interact with the Ran-exportin (Los1p.Gsp1p) machinery. These results provide the structural basis of Los1p.Gsp1p.Cex1p.tRNA complex formation, thus clarifying the dynamic mechanism of tRNA shuttling from exportin to the translational apparatus.

-	~~==Function==~~	+	Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm.,Nozawa K, Ishitani R, Yoshihisa T, Sato M, Arisaka F, Kanamaru S, Dohmae N, Mangroo D, Senger B, Becker HD, Nureki O Nucleic Acids Res. 2013 Apr 1;41(6):3901-14. doi: 10.1093/nar/gkt010. Epub 2013, Feb 8. PMID:23396276<ref>PMID:23396276</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org~~/~~uniprot~~/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:~~17203074~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3vwa]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023396276</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Ishitani, R.]]	+	[[Category: Ishitani, R]]
-	[[Category: Nozawa, K.]]	+	[[Category: Nozawa, K]]
-	[[Category: Nureki, O.]]	+	[[Category: Nureki, O]]
	[[Category: Heat repeat]]		[[Category: Heat repeat]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:39, 19 June 2013

OCA — Wed, 19 Jun 2013 13:39:43 GMT

←Older revision		Revision as of 13:39, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3vwa\| PDB=3vwa \| SCENE= }}
		+	===Crystal structure of Cex1p===
		+	{{ABSTRACT_PUBMED_23396276}}

-	~~The entry 3vwa is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref>

-	~~Authors~~: ~~Nozawa, K~~.~~, Ishitani, R~~.~~, Nureki, O~~.	+	==About this Structure==
		+	[[3vwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA].

-	~~Description~~: ~~Crystal structure of protein~~	+	==Reference==
		+	<ref group="xtra">PMID:023396276</ref><references group="xtra"/><references/>
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Ishitani, R.]]
		+	[[Category: Nozawa, K.]]
		+	[[Category: Nureki, O.]]
		+	[[Category: Heat repeat]]
		+	[[Category: Hydrolase]]
		+	[[Category: Kinase like domain]]
		+	[[Category: Nuclear export]]
		+	[[Category: Trna]]

OCA at 13:49, 28 November 2012

OCA — Wed, 28 Nov 2012 13:49:59 GMT

←Older revision		Revision as of 13:49, 28 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vwa is ON HOLD	+	The entry 3vwa is ON HOLD until Paper Publication

	Authors: Nozawa, K., Ishitani, R., Nureki, O.		Authors: Nozawa, K., Ishitani, R., Nureki, O.

	Description: Crystal structure of protein		Description: Crystal structure of protein

OCA: Protected "3vwa" [edit=sysop:move=sysop]

OCA — Wed, 22 Aug 2012 05:19:47 GMT

Protected "3vwa" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 22 August 2012

OCA: New page: '''Unreleased structure''' The entry 3vwa is ON HOLD Authors: Nozawa, K., Ishitani, R., Nureki, O. Description: Crystal structure of protein

OCA — Wed, 22 Aug 2012 05:19:46 GMT

New page: '''Unreleased structure''' The entry 3vwa is ON HOLD Authors: Nozawa, K., Ishitani, R., Nureki, O. Description: Crystal structure of protein

New page

'''Unreleased structure'''

The entry 3vwa is ON HOLD

Authors: Nozawa, K., Ishitani, R., Nureki, O.

Description: Crystal structure of protein