3vxk - Revision history

OCA at 12:40, 8 November 2023

OCA — Wed, 08 Nov 2023 12:40:56 GMT

←Older revision		Revision as of 12:40, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3vxk' size='340' side='right'caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vxk' size='340' side='right'caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Japanese_rice Japanese rice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VXK FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~D88~~, ~~Os03g0203200, LOC_Os03g10620 (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=39947 Japanese rice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [https://pdbe.org/3vxk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [https://www.ebi.ac.uk/pdbsum/3vxk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [https://pdbe.org/3vxk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [https://www.ebi.ac.uk/pdbsum/3vxk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D14_ORYSJ D14_ORYSJ] Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.<ref>PMID:19542179</ref> <ref>PMID:23301669</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Japanese rice]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hou, F]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: Miyakawa, T]]	+	[[Category: Hou F]]
-	[[Category: Qin, H M]]	+	[[Category: Miyakawa T]]
-	[[Category: Tanokura, M]]	+	[[Category: Qin H-M]]
-	[[Category: Xue, Y L]]	+	[[Category: Tanokura M]]
-	~~[[Category: Alpha/beta-hydrolase fold]]~~	+	[[Category: Xue Y-L]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 19:08, 27 July 2022

OCA — Wed, 27 Jul 2022 19:08:59 GMT

←Older revision		Revision as of 19:08, 27 July 2022
Line 1:		Line 1:

	==Crystal structure of OsD14==		==Crystal structure of OsD14==
-	<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3vxk' size='340' side='right'caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3VXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VXK FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [~~http~~://pdbe.org/3vxk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3vxk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [https://pdbe.org/3vxk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [https://www.ebi.ac.uk/pdbsum/3vxk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Japanese rice]]		[[Category: Japanese rice]]
		+	[[Category: Large Structures]]
	[[Category: Hou, F]]		[[Category: Hou, F]]
	[[Category: Miyakawa, T]]		[[Category: Miyakawa, T]]

OCA at 13:31, 11 August 2016

OCA — Thu, 11 Aug 2016 13:31:22 GMT

←Older revision		Revision as of 13:31, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of OsD14==		==Crystal structure of OsD14==
	<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VXK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VXK FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://pdbe.org/3vxk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vxk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3vxk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:30, 4 January 2015

OCA — Sun, 04 Jan 2015 10:30:30 GMT

←Older revision		Revision as of 10:30, 4 January 2015
Line 2:		Line 2:
	<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>	+	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VXK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 12:
	Molecular mechanism of strigolactone perception by DWARF14.,Nakamura H, Xue YL, Miyakawa T, Hou F, Qin HM, Fukui K, Shi X, Ito E, Ito S, Park SH, Miyauchi Y, Asano A, Totsuka N, Ueda T, Tanokura M, Asami T Nat Commun. 2013;4:2613. doi: 10.1038/ncomms3613. PMID:24131983<ref>PMID:24131983</ref>		Molecular mechanism of strigolactone perception by DWARF14.,Nakamura H, Xue YL, Miyakawa T, Hou F, Qin HM, Fukui K, Shi X, Ito E, Ito S, Park SH, Miyauchi Y, Asano A, Totsuka N, Ueda T, Tanokura M, Asami T Nat Commun. 2013;4:2613. doi: 10.1038/ncomms3613. PMID:24131983<ref>PMID:24131983</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Japanese rice]]		[[Category: Japanese rice]]
-	[[Category: Hou, F.]]	+	[[Category: Hou, F]]
-	[[Category: Miyakawa, T.]]	+	[[Category: Miyakawa, T]]
-	[[Category: Qin, H M.]]	+	[[Category: Qin, H M]]
-	[[Category: Tanokura, M.]]	+	[[Category: Tanokura, M]]
-	[[Category: Xue, Y L.]]	+	[[Category: Xue, Y L]]
	[[Category: Alpha/beta-hydrolase fold]]		[[Category: Alpha/beta-hydrolase fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:35, 1 May 2014

OCA — Thu, 01 May 2014 09:35:18 GMT

←Older revision		Revision as of 09:35, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>	+	<table><tr><td colspan='2'>[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D88, Os03g0203200, LOC_Os03g10620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.		Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.
Line 11:		Line 14:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:09, 30 April 2014

OCA — Wed, 30 Apr 2014 10:09:53 GMT

←Older revision		Revision as of 10:09, 30 April 2014
Line 4:		Line 4:
	[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>		[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vxk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vxk RCSB], [http://www.ebi.ac.uk/pdbsum/3vxk PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.		Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

OCA at 05:24, 30 April 2014

OCA — Wed, 30 Apr 2014 05:24:26 GMT

←Older revision		Revision as of 05:24, 30 April 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3vxk\| PDB~~=3vxk \| ~~SCENE~~= }}	+	==Crystal structure of OsD14==
-	===~~Crystal~~ structure ~~of OsD14~~===	+	<StructureSection load='3vxk' size='340' side='right' caption='[[3vxk]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA]. <br>
		+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== Publication Abstract from PubMed ==
		+	Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An alpha/beta-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

-	~~==About this Structure==~~	+	Molecular mechanism of strigolactone perception by DWARF14.,Nakamura H, Xue YL, Miyakawa T, Hou F, Qin HM, Fukui K, Shi X, Ito E, Ito S, Park SH, Miyauchi Y, Asano A, Totsuka N, Ueda T, Tanokura M, Asami T Nat Commun. 2013;4:2613. doi: 10.1038/ncomms3613. PMID:24131983<ref>PMID:24131983</ref>
-	~~[[3vxk]] is a 2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=3VXK OCA].~~	+
-	[[Category: ~~Oryza sativa japonica group~~]]	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Japanese rice]]
	[[Category: Hou, F.]]		[[Category: Hou, F.]]
	[[Category: Miyakawa, T.]]		[[Category: Miyakawa, T.]]

OCA at 08:14, 29 September 2013

OCA — Sun, 29 Sep 2013 08:14:34 GMT

←Older revision		Revision as of 08:14, 29 September 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3vxk\| PDB=3vxk \| SCENE= }}
		+	===Crystal structure of OsD14===

-	~~The entry~~ 3vxk is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[3vxk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VXK OCA].
-	~~Authors~~: ~~Xue, Y~~.-L.~~, Miyakawa, T~~., Hou, F., Qin, H.-M., Tanokura, M.	+	[[Category: Oryza sativa japonica group]]
-		+	[[Category: Hou, F.]]
-	~~Description~~: ~~Crystal structure of OsD14~~	+	[[Category: Miyakawa, T.]]
		+	[[Category: Qin, H M.]]
		+	[[Category: Tanokura, M.]]
		+	[[Category: Xue, Y L.]]
		+	[[Category: Alpha/beta-hydrolase fold]]
		+	[[Category: Hydrolase]]

OCA at 10:16, 5 December 2012

OCA — Wed, 05 Dec 2012 10:16:49 GMT

←Older revision		Revision as of 10:16, 5 December 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3vxk is ON HOLD	+	The entry 3vxk is ON HOLD until Paper Publication

	Authors: Xue, Y.-L., Miyakawa, T., Hou, F., Qin, H.-M., Tanokura, M.		Authors: Xue, Y.-L., Miyakawa, T., Hou, F., Qin, H.-M., Tanokura, M.

	Description: Crystal structure of OsD14		Description: Crystal structure of OsD14

OCA at 06:40, 10 October 2012

OCA — Wed, 10 Oct 2012 06:40:29 GMT

←Older revision		Revision as of 06:40, 10 October 2012
Line 3:		Line 3:
	The entry 3vxk is ON HOLD		The entry 3vxk is ON HOLD

-	Authors: Xue, Y.-L., Miyakawa, T., Hou, F., Qin H.-M., Tanokura, M.	+	Authors: Xue, Y.-L., Miyakawa, T., Hou, F., Qin, H.-M., Tanokura, M.

	Description: Crystal structure of OsD14		Description: Crystal structure of OsD14