3w54 - Revision history

OCA at 08:43, 20 March 2024

OCA — Wed, 20 Mar 2024 08:43:58 GMT

←Older revision		Revision as of 08:43, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3w54' size='340' side='right'caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3w54' size='340' side='right'caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Trybb Trybb~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W54 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W54 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vv9\|3vv9]], [[3vva\|3vva]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 TRYBB])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [https://pdbe.org/3w54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [https://www.ebi.ac.uk/pdbsum/3w54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w54 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [https://pdbe.org/3w54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [https://www.ebi.ac.uk/pdbsum/3w54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w54 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.	+	[https://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	In addition to haem copper oxidases, all higher plants, some algae, yeasts, molds, metazoans, and pathogenic microorganisms such as Trypanosoma brucei contain an additional terminal oxidase, the cyanide-insensitive alternative oxidase (AOX). AOX is a diiron carboxylate protein that catalyzes the four-electron reduction of dioxygen to water by ubiquinol. In T. brucei, a parasite that causes human African sleeping sickness, AOX plays a critical role in the survival of the parasite in its bloodstream form. Because AOX is absent from mammals, this protein represents a unique and promising therapeutic target. Despite its bioenergetic and medical importance, however, structural features of any AOX are yet to be elucidated. Here we report crystal structures of the trypanosomal alternative oxidase in the absence and presence of ascofuranone derivatives. All structures reveal that the oxidase is a homodimer with the nonhaem diiron carboxylate active site buried within a four-helix bundle. Unusually, the active site is ligated solely by four glutamate residues in its oxidized inhibitor-free state; however, inhibitor binding induces the ligation of a histidine residue. A highly conserved Tyr220 is within 4 A of the active site and is critical for catalytic activity. All structures also reveal that there are two hydrophobic cavities per monomer. Both inhibitors bind to one cavity within 4 A and 5 A of the active site and Tyr220, respectively. A second cavity interacts with the inhibitor-binding cavity at the diiron center. We suggest that both cavities bind ubiquinol and along with Tyr220 are required for the catalytic cycle for O2 reduction.	+
-		+
-	Structure of the trypanosome cyanide-insensitive alternative oxidase.,Shiba T, Kido Y, Sakamoto K, Inaoka DK, Tsuge C, Tatsumi R, Takahashi G, Balogun EO, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Saimoto H, Moore AL, Harada S, Kita K Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4580-5. doi:, 10.1073/pnas.1218386110. Epub 2013 Mar 4. PMID:23487766<ref>PMID:23487766</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3w54" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Trybb~~]]	+	[[Category: Trypanosoma brucei brucei]]
-	[[Category: Aoki, T]]	+	[[Category: Aoki T]]
-	[[Category: Balogun~~, E O~~]]	+	[[Category: Balogun EO]]
-	[[Category: Harada, S]]	+	[[Category: Harada S]]
-	[[Category: Honma, T]]	+	[[Category: Honma T]]
-	[[Category: Inaoka~~, D K~~]]	+	[[Category: Inaoka DK]]
-	[[Category: Inoue, M]]	+	[[Category: Inoue M]]
-	[[Category: Kido, Y]]	+	[[Category: Kido Y]]
-	[[Category: Kita, K]]	+	[[Category: Kita K]]
-	[[Category: Matsuoka, S]]	+	[[Category: Matsuoka S]]
-	[[Category: Moore~~, A L~~]]	+	[[Category: Moore AL]]
-	[[Category: Nara, T]]	+	[[Category: Nara T]]
-	[[Category: Saimoto, H]]	+	[[Category: Saimoto H]]
-	[[Category: Sakamoto, K]]	+	[[Category: Sakamoto K]]
-	[[Category: Shiba, T]]	+	[[Category: Shiba T]]
-	[[Category: Takahashi, G]]	+	[[Category: Takahashi G]]
-	[[Category: Tanaka, A]]	+	[[Category: Tanaka A]]
-	[[Category: Tatsumi, R]]	+	[[Category: Tatsumi R]]
-	[[Category: Tsuge, C]]	+	[[Category: Tsuge C]]
-	~~[[Category: Alternative oxidase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Membrane bound diiron protein]]~~	+
-	~~[[Category: Oxidase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase-oxido complex]]~~	+
-	~~[[Category: Oxidoreductase-oxidoreductase inhibitor complex~~]]	+

OCA at 05:22, 3 August 2022

OCA — Wed, 03 Aug 2022 05:22:41 GMT

←Older revision		Revision as of 05:22, 3 August 2022
Line 1:		Line 1:

	==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B==		==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B==
-	<StructureSection load='3w54' size='340' side='right' caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3w54' size='340' side='right'caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3W54 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W54 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vv9\|3vv9]], [[3vva\|3vva]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vv9\|3vv9]], [[3vva\|3vva]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 TRYBB])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 TRYBB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [~~http~~://pdbe.org/3w54 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3w54 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3w54 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [https://pdbe.org/3w54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [https://www.ebi.ac.uk/pdbsum/3w54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w54 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.	+	[[https://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Trybb]]		[[Category: Trybb]]
	[[Category: Aoki, T]]		[[Category: Aoki, T]]

OCA at 14:19, 11 August 2016

OCA — Thu, 11 Aug 2016 14:19:00 GMT

←Older revision		Revision as of 14:19, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B==		==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B==
	<StructureSection load='3w54' size='340' side='right' caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3w54' size='340' side='right' caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trypanosoma_brucei_brucei Trypanosoma brucei brucei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W54 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trybb Trybb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W54 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vv9\|3vv9]], [[3vva\|3vva]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vv9\|3vv9]], [[3vva\|3vva]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 ~~Trypanosoma brucei brucei~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 TRYBB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [http://www.ebi.ac.uk/pdbsum/3w54 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [http://pdbe.org/3w54 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [http://www.ebi.ac.uk/pdbsum/3w54 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w54 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AOX_TRYBB AOX_TRYBB]] Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3w54" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Trypanosoma brucei brucei~~]]	+	[[Category: Trybb]]
	[[Category: Aoki, T]]		[[Category: Aoki, T]]
	[[Category: Balogun, E O]]		[[Category: Balogun, E O]]

OCA at 12:32, 5 January 2015

OCA — Mon, 05 Jan 2015 12:32:05 GMT

←Older revision		Revision as of 12:32, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3w54\| PDB=3w54 \| SCENE= }}~~	+	==Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B==
-	===Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B===	+	<StructureSection load='3w54' size='340' side='right' caption='[[3w54]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23487766}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3w54]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W54 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=RNB:3-CHLORO-5-[(2E)-3,7-DIMETHYLOCTA-2,6-DIEN-1-YL]-4,6-DIHYDROXY-2-METHYLBENZALDEHYDE'>RNB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vv9\|3vv9]], [[3vva\|3vva]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AOX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 Trypanosoma brucei brucei])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w54 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w54 RCSB], [http://www.ebi.ac.uk/pdbsum/3w54 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In addition to haem copper oxidases, all higher plants, some algae, yeasts, molds, metazoans, and pathogenic microorganisms such as Trypanosoma brucei contain an additional terminal oxidase, the cyanide-insensitive alternative oxidase (AOX). AOX is a diiron carboxylate protein that catalyzes the four-electron reduction of dioxygen to water by ubiquinol. In T. brucei, a parasite that causes human African sleeping sickness, AOX plays a critical role in the survival of the parasite in its bloodstream form. Because AOX is absent from mammals, this protein represents a unique and promising therapeutic target. Despite its bioenergetic and medical importance, however, structural features of any AOX are yet to be elucidated. Here we report crystal structures of the trypanosomal alternative oxidase in the absence and presence of ascofuranone derivatives. All structures reveal that the oxidase is a homodimer with the nonhaem diiron carboxylate active site buried within a four-helix bundle. Unusually, the active site is ligated solely by four glutamate residues in its oxidized inhibitor-free state; however, inhibitor binding induces the ligation of a histidine residue. A highly conserved Tyr220 is within 4 A of the active site and is critical for catalytic activity. All structures also reveal that there are two hydrophobic cavities per monomer. Both inhibitors bind to one cavity within 4 A and 5 A of the active site and Tyr220, respectively. A second cavity interacts with the inhibitor-binding cavity at the diiron center. We suggest that both cavities bind ubiquinol and along with Tyr220 are required for the catalytic cycle for O2 reduction.

-	~~==About this~~ Structure==	+	Structure of the trypanosome cyanide-insensitive alternative oxidase.,Shiba T, Kido Y, Sakamoto K, Inaoka DK, Tsuge C, Tatsumi R, Takahashi G, Balogun EO, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Saimoto H, Moore AL, Harada S, Kita K Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4580-5. doi:, 10.1073/pnas.1218386110. Epub 2013 Mar 4. PMID:23487766<ref>PMID:23487766</ref>
-	~~[[3w54]] is a 4 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~3W54 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Trypanosoma brucei brucei]]		[[Category: Trypanosoma brucei brucei]]
-	[[Category: Aoki, T.]]	+	[[Category: Aoki, T]]
-	[[Category: Balogun, E O.]]	+	[[Category: Balogun, E O]]
-	[[Category: Harada, S.]]	+	[[Category: Harada, S]]
-	[[Category: Honma, T.]]	+	[[Category: Honma, T]]
-	[[Category: Inaoka, D K.]]	+	[[Category: Inaoka, D K]]
-	[[Category: Inoue, M.]]	+	[[Category: Inoue, M]]
-	[[Category: Kido, Y.]]	+	[[Category: Kido, Y]]
-	[[Category: Kita, K.]]	+	[[Category: Kita, K]]
-	[[Category: Matsuoka, S.]]	+	[[Category: Matsuoka, S]]
-	[[Category: Moore, A L.]]	+	[[Category: Moore, A L]]
-	[[Category: Nara, T.]]	+	[[Category: Nara, T]]
-	[[Category: Saimoto, H.]]	+	[[Category: Saimoto, H]]
-	[[Category: Sakamoto, K.]]	+	[[Category: Sakamoto, K]]
-	[[Category: Shiba, T.]]	+	[[Category: Shiba, T]]
-	[[Category: Takahashi, G.]]	+	[[Category: Takahashi, G]]
-	[[Category: Tanaka, A.]]	+	[[Category: Tanaka, A]]
-	[[Category: Tatsumi, R.]]	+	[[Category: Tatsumi, R]]
-	[[Category: Tsuge, C.]]	+	[[Category: Tsuge, C]]
	[[Category: Alternative oxidase]]		[[Category: Alternative oxidase]]
	[[Category: Membrane]]		[[Category: Membrane]]

OCA at 07:50, 27 March 2013

OCA — Wed, 27 Mar 2013 07:50:49 GMT

←Older revision		Revision as of 07:50, 27 March 2013
Line 1:		Line 1:
	{{STRUCTURE_3w54\| PDB=3w54 \| SCENE= }}		{{STRUCTURE_3w54\| PDB=3w54 \| SCENE= }}
	===Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B===		===Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B===
		+	{{ABSTRACT_PUBMED_23487766}}

	==About this Structure==		==About this Structure==

OCA at 10:26, 13 March 2013

OCA — Wed, 13 Mar 2013 10:26:40 GMT

←Older revision		Revision as of 10:26, 13 March 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3w54\| PDB=3w54 \| SCENE= }}
		+	===Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B===

-	~~The entry~~ 3w54 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[3w54]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W54 OCA].
-	~~Authors~~: ~~Shiba, T~~.~~, Kido, Y~~.~~, Sakamoto, K~~.~~, Inaoka, D~~.K.~~, Tsuge, C~~.~~, Tatsumi, R~~., ~~Takahashi, G~~., Balogun, E.O., ~~Nara, T~~.~~, Aoki, T.,~~ Honma, T., ~~Tanaka, A~~., Inoue, M., Matsuoka, S., Saimoto, H., ~~Moore~~, A.L., ~~Harada~~, S., ~~Kita, K~~.	+	[[Category: Trypanosoma brucei brucei]]
-		+	[[Category: Aoki, T.]]
-	~~Description~~: ~~Crystal structure of cyanide~~-~~insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B~~	+	[[Category: Balogun, E O.]]
		+	[[Category: Harada, S.]]
		+	[[Category: Honma, T.]]
		+	[[Category: Inaoka, D K.]]
		+	[[Category: Inoue, M.]]
		+	[[Category: Kido, Y.]]
		+	[[Category: Kita, K.]]
		+	[[Category: Matsuoka, S.]]
		+	[[Category: Moore, A L.]]
		+	[[Category: Nara, T.]]
		+	[[Category: Saimoto, H.]]
		+	[[Category: Sakamoto, K.]]
		+	[[Category: Shiba, T.]]
		+	[[Category: Takahashi, G.]]
		+	[[Category: Tanaka, A.]]
		+	[[Category: Tatsumi, R.]]
		+	[[Category: Tsuge, C.]]
		+	[[Category: Alternative oxidase]]
		+	[[Category: Membrane]]
		+	[[Category: Membrane bound diiron protein]]
		+	[[Category: Oxidase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxidoreductase-oxido complex]]
		+	[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]

OCA at 13:32, 6 March 2013

OCA — Wed, 06 Mar 2013 13:32:48 GMT

←Older revision		Revision as of 13:32, 6 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3w54 is ON HOLD	+	The entry 3w54 is ON HOLD until Paper Publication

	Authors: Shiba, T., Kido, Y., Sakamoto, K., Inaoka, D.K., Tsuge, C., Tatsumi, R., Takahashi, G., Balogun, E.O., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka, S., Saimoto, H., Moore, A.L., Harada, S., Kita, K.		Authors: Shiba, T., Kido, Y., Sakamoto, K., Inaoka, D.K., Tsuge, C., Tatsumi, R., Takahashi, G., Balogun, E.O., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka, S., Saimoto, H., Moore, A.L., Harada, S., Kita, K.

	Description: Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B		Description: Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B

OCA: Protected "3w54" [edit=sysop:move=sysop]

OCA — Wed, 30 Jan 2013 11:47:26 GMT

Protected "3w54" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 30 January 2013

OCA: New page: '''Unreleased structure''' The entry 3w54 is ON HOLD Authors: Shiba, T., Kido, Y., Sakamoto, K., Inaoka, D.K., Tsuge, C., Tatsumi, R., Takahashi, G., Balogun, E.O., Nara, T., Aoki, T., ...

OCA — Wed, 30 Jan 2013 11:47:25 GMT

New page: '''Unreleased structure''' The entry 3w54 is ON HOLD Authors: Shiba, T., Kido, Y., Sakamoto, K., Inaoka, D.K., Tsuge, C., Tatsumi, R., Takahashi, G., Balogun, E.O., Nara, T., Aoki, T., ...

New page

'''Unreleased structure'''

The entry 3w54 is ON HOLD

Authors: Shiba, T., Kido, Y., Sakamoto, K., Inaoka, D.K., Tsuge, C., Tatsumi, R., Takahashi, G., Balogun, E.O., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka, S., Saimoto, H., Moore, A.L., Harada, S., Kita, K.

Description: Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with colletochlorin B