3wa0 - Revision history

OCA at 13:00, 8 November 2023

OCA — Wed, 08 Nov 2023 13:00:48 GMT

←Older revision		Revision as of 13:00, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of merlin complexed with DCAF1/VprBP==		==Crystal structure of merlin complexed with DCAF1/VprBP==
-	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]]' scene=''>	+	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.31Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MERL_MOUSE MERL_MOUSE] Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.<ref>PMID:20181838</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Merlin, a tumor suppressor encoded by the neurofibromatosis type 2 gene, has been shown to suppress tumorigenesis by inhibiting the Cullin 4-RING E3 ubiquitin ligase CRL4DCAF 1 in the nucleus. This inhibition is mediated by direct binding of merlin to DDB1-and-Cullin 4-associated Factor 1 (DCAF1), yet the binding mode of merlin to DCAF1 is not well defined. Here, we report structural and biophysical studies of the merlin binding to DCAF1 and its interference with CD44 binding. The crystal structure of the merlin FERM domain bound to the DCAF1 C-terminal acidic tail reveals that the hydrophobic IILXLN motif located at the C-terminal end of DCAF1 binds subdomain C of the FERM domain by forming a beta-strand. The binding site and mode resemble that of merlin binding to the CD44 cytoplasmic tail. Competition binding assay showed that CD44 and DCAF1 compete for binding to the merlin FERM domain in solution. The CD44 cytoplasmic tail is known to be cleaved for nuclear translocation by regulated intra-membrane proteolysis (RIP). Our structure implies that, in the nucleus, the CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis.
		+
		+	Structural basis of DDB1-and-Cullin 4-associated Factor 1 (DCAF1) recognition by merlin/NF2 and its implication in tumorigenesis by CD44-mediated inhibition of merlin suppression of DCAF1 function.,Mori T, Gotoh S, Shirakawa M, Hakoshima T Genes Cells. 2014 Jun 9. doi: 10.1111/gtc.12161. PMID:24912773<ref>PMID:24912773</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wa0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 11:		Line 23:
	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]		*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	*[[VprBP\|VprBP]]		*[[VprBP\|VprBP]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
	[[Category: Gotoh S]]		[[Category: Gotoh S]]
	[[Category: Hakoshima T]]		[[Category: Hakoshima T]]
	[[Category: Mori T]]		[[Category: Mori T]]
	[[Category: Shirakawa M]]		[[Category: Shirakawa M]]

OCA at 10:29, 31 August 2022

OCA — Wed, 31 Aug 2022 10:29:05 GMT

←Older revision		Revision as of 10:29, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of merlin complexed with DCAF1/VprBP==		==Crystal structure of merlin complexed with DCAF1/VprBP==
-	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]]~~, [[Resolution\|resolution]] 2.31Å~~' scene=''>	+	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3wa0]] is a 11 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
-	</td></tr>~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</div></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/MERL_MOUSE MERL_MOUSE]] Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.<ref>PMID:20181838</ref> [[https://www.uniprot.org/uniprot/VPRBP_HUMAN VPRBP_HUMAN]] Component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, VprBP/DCAF1 may function as the substrate recognition module within this complex. For example, VprBP/DCAF1 targets NF2 to the E3 ubiquitin-ligase complex for ubiquitination and subsequent proteasome-dependent degradation. In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1 function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1 function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. Associated with chromatin in a DDB1-independent and cell cycle-dependent manner, VprBP/DCAF1 is recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.<ref>PMID:17314515</ref> <ref>PMID:17620334</ref> <ref>PMID:17626091</ref> <ref>PMID:17630831</ref> <ref>PMID:17609381</ref> <ref>PMID:17559673</ref> <ref>PMID:18524771</ref> <ref>PMID:18606781</ref> <ref>PMID:18332868</ref> <ref>PMID:18464893</ref> <ref>PMID:19264781</ref> <ref>PMID:19923175</ref> <ref>PMID:23063525</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Merlin, a tumor suppressor encoded by the neurofibromatosis type 2 gene, has been shown to suppress tumorigenesis by inhibiting the Cullin 4-RING E3 ubiquitin ligase CRL4DCAF 1 in the nucleus. This inhibition is mediated by direct binding of merlin to DDB1-and-Cullin 4-associated Factor 1 (DCAF1), yet the binding mode of merlin to DCAF1 is not well defined. Here, we report structural and biophysical studies of the merlin binding to DCAF1 and its interference with CD44 binding. The crystal structure of the merlin FERM domain bound to the DCAF1 C-terminal acidic tail reveals that the hydrophobic IILXLN motif located at the C-terminal end of DCAF1 binds subdomain C of the FERM domain by forming a beta-strand. The binding site and mode resemble that of merlin binding to the CD44 cytoplasmic tail. Competition binding assay showed that CD44 and DCAF1 compete for binding to the merlin FERM domain in solution. The CD44 cytoplasmic tail is known to be cleaved for nuclear translocation by regulated intra-membrane proteolysis (RIP). Our structure implies that, in the nucleus, the CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis.
-
-	Structural basis of DDB1-and-Cullin 4-associated Factor 1 (DCAF1) recognition by merlin/NF2 and its implication in tumorigenesis by CD44-mediated inhibition of merlin suppression of DCAF1 function.,Mori T, Gotoh S, Shirakawa M, Hakoshima T Genes Cells. 2014 Jun 9. doi: 10.1111/gtc.12161. PMID:24912773<ref>PMID:24912773</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3wa0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 11:
	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]		*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	*[[VprBP\|VprBP]]		*[[VprBP\|VprBP]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gotoh, S]]	+	[[Category: Gotoh S]]
-	[[Category: Hakoshima, T]]	+	[[Category: Hakoshima T]]
-	[[Category: Mori, T]]	+	[[Category: Mori T]]
-	[[Category: Shirakawa, M]]	+	[[Category: Shirakawa M]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Merlin ferm domain~~]]	+

OCA at 04:56, 25 August 2022

OCA — Thu, 25 Aug 2022 04:56:53 GMT

←Older revision		Revision as of 04:56, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nf2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), DCAF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
	</table>		</table>
Line 29:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lk3 transgenic mice]]
	[[Category: Gotoh, S]]		[[Category: Gotoh, S]]
	[[Category: Hakoshima, T]]		[[Category: Hakoshima, T]]

OCA at 05:28, 3 August 2022

OCA — Wed, 03 Aug 2022 05:28:39 GMT

←Older revision		Revision as of 05:28, 3 August 2022
Line 1:		Line 1:

	==Crystal structure of merlin complexed with DCAF1/VprBP==		==Crystal structure of merlin complexed with DCAF1/VprBP==
-	<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>	+	<StructureSection load='3wa0' size='340' side='right'caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human] and [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nf2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), DCAF1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nf2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), DCAF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [~~http~~://pdbe.org/3wa0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [https://pdbe.org/3wa0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MERL_MOUSE MERL_MOUSE]] Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.<ref>PMID:20181838</ref> [[~~http~~://www.uniprot.org/uniprot/VPRBP_HUMAN VPRBP_HUMAN]] Component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, VprBP/DCAF1 may function as the substrate recognition module within this complex. For example, VprBP/DCAF1 targets NF2 to the E3 ubiquitin-ligase complex for ubiquitination and subsequent proteasome-dependent degradation. In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1 function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1 function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. Associated with chromatin in a DDB1-independent and cell cycle-dependent manner, VprBP/DCAF1 is recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.<ref>PMID:17314515</ref> <ref>PMID:17620334</ref> <ref>PMID:17626091</ref> <ref>PMID:17630831</ref> <ref>PMID:17609381</ref> <ref>PMID:17559673</ref> <ref>PMID:18524771</ref> <ref>PMID:18606781</ref> <ref>PMID:18332868</ref> <ref>PMID:18464893</ref> <ref>PMID:19264781</ref> <ref>PMID:19923175</ref> <ref>PMID:23063525</ref>	+	[[https://www.uniprot.org/uniprot/MERL_MOUSE MERL_MOUSE]] Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.<ref>PMID:20181838</ref> [[https://www.uniprot.org/uniprot/VPRBP_HUMAN VPRBP_HUMAN]] Component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, VprBP/DCAF1 may function as the substrate recognition module within this complex. For example, VprBP/DCAF1 targets NF2 to the E3 ubiquitin-ligase complex for ubiquitination and subsequent proteasome-dependent degradation. In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1 function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1 function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. Associated with chromatin in a DDB1-independent and cell cycle-dependent manner, VprBP/DCAF1 is recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.<ref>PMID:17314515</ref> <ref>PMID:17620334</ref> <ref>PMID:17626091</ref> <ref>PMID:17630831</ref> <ref>PMID:17609381</ref> <ref>PMID:17559673</ref> <ref>PMID:18524771</ref> <ref>PMID:18606781</ref> <ref>PMID:18332868</ref> <ref>PMID:18464893</ref> <ref>PMID:19264781</ref> <ref>PMID:19923175</ref> <ref>PMID:23063525</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	==See Also==		==See Also==
	*[[Merlin\|Merlin]]		*[[Merlin\|Merlin]]
-	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	*[[VprBP\|VprBP]]		*[[VprBP\|VprBP]]
	== References ==		== References ==
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Gotoh, S]]		[[Category: Gotoh, S]]

OCA at 09:22, 21 February 2019

OCA — Thu, 21 Feb 2019 09:22:02 GMT

←Older revision		Revision as of 09:22, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nf2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), DCAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://pdbe.org/3wa0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://pdbe.org/3wa0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3wa0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3wa0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Merlin\|Merlin]]
		+	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
		+	*[[VprBP\|VprBP]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Gotoh, S]]		[[Category: Gotoh, S]]
	[[Category: Hakoshima, T]]		[[Category: Hakoshima, T]]

OCA at 08:33, 5 August 2016

OCA — Fri, 05 Aug 2016 08:33:07 GMT

←Older revision		Revision as of 08:33, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of merlin complexed with DCAF1/VprBP==		==Crystal structure of merlin complexed with DCAF1/VprBP==
	<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://pdbe.org/3wa0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wa0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wa0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:18, 25 December 2014

OCA — Thu, 25 Dec 2014 17:18:24 GMT

←Older revision		Revision as of 17:18, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MERL_MOUSE MERL_MOUSE]] Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.<ref>PMID:20181838</ref> [[http://www.uniprot.org/uniprot/VPRBP_HUMAN VPRBP_HUMAN]] Component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex, VprBP/DCAF1 may function as the substrate recognition module within this complex. For example, VprBP/DCAF1 targets NF2 to the E3 ubiquitin-ligase complex for ubiquitination and subsequent proteasome-dependent degradation. In case of infection by HIV-1 virus, it is recruited by HIV-1 Vpr in order to hijack the CUL4A-RBX1-DDB1 function leading to arrest the cell cycle in G2 phase, and also to protect the viral protein from proteasomal degradation by another E3 ubiquitin ligase. In case of infection by HIV-2 virus, it is recruited by HIV-2 Vpx in order to hijack the CUL4A-RBX1-DDB1 function leading to enhanced efficiency of macrophage infection and promotion of the replication of cognate primate lentiviruses in cells of monocyte/macrophage lineage. Associated with chromatin in a DDB1-independent and cell cycle-dependent manner, VprBP/DCAF1 is recruited to chromatin as DNA is being replicated and is released from chromatin before mitosis.<ref>PMID:17314515</ref> <ref>PMID:17620334</ref> <ref>PMID:17626091</ref> <ref>PMID:17630831</ref> <ref>PMID:17609381</ref> <ref>PMID:17559673</ref> <ref>PMID:18524771</ref> <ref>PMID:18606781</ref> <ref>PMID:18332868</ref> <ref>PMID:18464893</ref> <ref>PMID:19264781</ref> <ref>PMID:19923175</ref> <ref>PMID:23063525</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Gotoh, S.]]	+	[[Category: Gotoh, S]]
-	[[Category: Hakoshima, T.]]	+	[[Category: Hakoshima, T]]
-	[[Category: Mori, T.]]	+	[[Category: Mori, T]]
-	[[Category: Shirakawa, M.]]	+	[[Category: Shirakawa, M]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Merlin ferm domain]]		[[Category: Merlin ferm domain]]

OCA at 05:27, 25 June 2014

OCA — Wed, 25 Jun 2014 05:27:33 GMT

←Older revision		Revision as of 05:27, 25 June 2014
Line 1:		Line 1:
-	==~~Merlin complex~~==	+	==Crystal structure of merlin complexed with DCAF1/VprBP==
	<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 7:		Line 7:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Merlin, a tumor suppressor encoded by the neurofibromatosis type 2 gene, has been shown to suppress tumorigenesis by inhibiting the Cullin 4-RING E3 ubiquitin ligase CRL4DCAF 1 in the nucleus. This inhibition is mediated by direct binding of merlin to DDB1-and-Cullin 4-associated Factor 1 (DCAF1), yet the binding mode of merlin to DCAF1 is not well defined. Here, we report structural and biophysical studies of the merlin binding to DCAF1 and its interference with CD44 binding. The crystal structure of the merlin FERM domain bound to the DCAF1 C-terminal acidic tail reveals that the hydrophobic IILXLN motif located at the C-terminal end of DCAF1 binds subdomain C of the FERM domain by forming a beta-strand. The binding site and mode resemble that of merlin binding to the CD44 cytoplasmic tail. Competition binding assay showed that CD44 and DCAF1 compete for binding to the merlin FERM domain in solution. The CD44 cytoplasmic tail is known to be cleaved for nuclear translocation by regulated intra-membrane proteolysis (RIP). Our structure implies that, in the nucleus, the CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis.
		+
		+	Structural basis of DDB1-and-Cullin 4-associated Factor 1 (DCAF1) recognition by merlin/NF2 and its implication in tumorigenesis by CD44-mediated inhibition of merlin suppression of DCAF1 function.,Mori T, Gotoh S, Shirakawa M, Hakoshima T Genes Cells. 2014 Jun 9. doi: 10.1111/gtc.12161. PMID:24912773<ref>PMID:24912773</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:52, 28 May 2014

OCA — Wed, 28 May 2014 09:52:41 GMT

←Older revision		Revision as of 09:52, 28 May 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Merlin complex==
-		+	<StructureSection load='3wa0' size='340' side='right' caption='[[3wa0]], [[Resolution\|resolution]] 2.31Å' scene=''>
-	~~The entry~~ 3wa0 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3wa0]] is a 11 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WA0 FirstGlance]. <br>
-	~~Authors~~: ~~Mori~~, T., ~~Gotoh~~, S., ~~Shirakawa~~, M., Hakoshima, T.	+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2zpy\|2zpy]], [[1isn\|1isn]], [[1j19\|1j19]], [[2yvc\|2yvc]]</td></tr>
-	~~Description~~: Merlin ~~complex~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wa0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wa0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wa0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wa0 PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gotoh, S.]]
		+	[[Category: Hakoshima, T.]]
		+	[[Category: Mori, T.]]
		+	[[Category: Shirakawa, M.]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Merlin ferm domain]]

OCA at 06:38, 16 May 2013

OCA — Thu, 16 May 2013 06:38:17 GMT

←Older revision		Revision as of 06:38, 16 May 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wa0 is ON HOLD	+	The entry 3wa0 is ON HOLD until Paper Publication

	Authors: Mori, T., Gotoh, S., Shirakawa, M., Hakoshima, T.		Authors: Mori, T., Gotoh, S., Shirakawa, M., Hakoshima, T.

	Description: Merlin complex		Description: Merlin complex