3wgw - Revision history

OCA at 13:10, 8 November 2023

OCA — Wed, 08 Nov 2023 13:10:35 GMT

←Older revision		Revision as of 13:10, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3wgw' size='340' side='right'caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3wgw' size='340' side='right'caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WGW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [https://pdbe.org/3wgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [https://www.ebi.ac.uk/pdbsum/3wgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [https://pdbe.org/3wgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [https://www.ebi.ac.uk/pdbsum/3wgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>	+	[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hashimoto, H]]	+	[[Category: Hashimoto H]]
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Dna binding protein-inhibitor complex~~]]	+

OCA at 05:36, 3 August 2022

OCA — Wed, 03 Aug 2022 05:36:00 GMT

←Older revision		Revision as of 05:36, 3 August 2022
Line 3:		Line 3:
	<StructureSection load='3wgw' size='340' side='right'caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3wgw' size='340' side='right'caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WGW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [~~http~~://pdbe.org/3wgw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wgw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [https://pdbe.org/3wgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [https://www.ebi.ac.uk/pdbsum/3wgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>	+	[[https://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:56, 25 December 2019

OCA — Wed, 25 Dec 2019 09:56:42 GMT

←Older revision		Revision as of 09:56, 25 December 2019
Line 1:		Line 1:

	==Structure of PCNA bound to a small molecule inhibitor==		==Structure of PCNA bound to a small molecule inhibitor==
-	<StructureSection load='3wgw' size='340' side='right' caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3wgw' size='340' side='right'caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WGW FirstGlance]. <br>		<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WGW FirstGlance]. <br>
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>		[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Small molecule inhibitors of proliferating cell nuclear antigen (PCNA)/PCNA interacting protein box (PIP-Box) interactions, including T2 amino alcohol (T2AA), inhibit translesion DNA synthesis. The crystal structure of PCNA in complex with T2AA revealed that T2AA bound to the surface adjacent to the subunit interface of the homotrimer of PCNA in addition to the PIP-box binding cavity. Because this site is close to Lys-164, which is monoubiquitinated by RAD18, we postulated that T2AA would affect monoubiquitinated PCNA interactions. Binding of monoubiquitinated PCNA and a purified pol eta fragment containing the UBZ and PIP-box was inhibited by T2AA in vitro. T2AA decreased PCNA/pol eta and PCNA/REV1 chromatin colocalization but did not inhibit PCNA monoubiquitination, suggesting that T2AA hinders interactions of pol eta and REV1 with monoubiquitinated PCNA. Interstrand DNA cross-links (ICLs) are repaired by mechanisms using translesion DNA synthesis that is regulated by monoubiquitinated PCNA. T2AA significantly delayed reactivation of a reporter plasmid containing an ICL. Neutral comet analysis of cells receiving T2AA in addition to cisplatin revealed that T2AA significantly enhanced formation of DNA double strand breaks (DSBs) by cisplatin. T2AA promoted colocalized foci formation of phospho-ATM and 53BP1 and up-regulated phospho-BRCA1 in cisplatin-treated cells, suggesting that T2AA increases DSBs. When cells were treated by cisplatin and T2AA, their clonogenic survival was significantly less than that of those treated by cisplatin only. These findings show that the inhibitors of monoubiquitinated PCNA chemosensitize cells by inhibiting repair of ICLs and DSBs.
		+
		+	A small molecule inhibitor of monoubiquitinated Proliferating Cell Nuclear Antigen (PCNA) inhibits repair of interstrand DNA cross-link, enhances DNA double strand break, and sensitizes cancer cells to cisplatin.,Inoue A, Kikuchi S, Hishiki A, Shao Y, Heath R, Evison BJ, Actis M, Canman CE, Hashimoto H, Fujii N J Biol Chem. 2014 Mar 7;289(10):7109-20. doi: 10.1074/jbc.M113.520429. Epub 2014 , Jan 28. PMID:24474685<ref>PMID:24474685</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wgw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Proliferating ~~Cell Nuclear Antigen~~\|Proliferating ~~Cell Nuclear Antigen~~]]	+	*[[Proliferating cell nuclear antigen 3D structures\|Proliferating cell nuclear antigen 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 18:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hashimoto, H]]		[[Category: Hashimoto, H]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
	[[Category: Dna binding protein-inhibitor complex]]		[[Category: Dna binding protein-inhibitor complex]]

OCA at 13:25, 5 August 2016

OCA — Fri, 05 Aug 2016 13:25:43 GMT

←Older revision		Revision as of 13:25, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of PCNA bound to a small molecule inhibitor==		==Structure of PCNA bound to a small molecule inhibitor==
	<StructureSection load='3wgw' size='340' side='right' caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3wgw' size='340' side='right' caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [http://www.ebi.ac.uk/pdbsum/3wgw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [http://pdbe.org/3wgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [http://www.ebi.ac.uk/pdbsum/3wgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 02:50, 25 December 2014

OCA — Thu, 25 Dec 2014 02:50:48 GMT

←Older revision		Revision as of 02:50, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3wgw\| PDB=3wgw \| SCENE= }}~~	+	==Structure of PCNA bound to a small molecule inhibitor==
-	===Structure of PCNA bound to a small molecule inhibitor===	+	<StructureSection load='3wgw' size='340' side='right' caption='[[3wgw]], [[Resolution\|resolution]] 2.80Å' scene=''>
-		+	== Structural highlights ==
-	==Function==	+	<table><tr><td colspan='2'>[[3wgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WGW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T2B:4-{4-[(2S)-2-AMINO-3-HYDROXYPROPYL]-2,6-DIIODOPHENOXY}PHENOL'>T2B</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PCNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wgw RCSB], [http://www.ebi.ac.uk/pdbsum/3wgw PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>		[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>

-	==~~About this Structure~~==	+	==See Also==
-	[[~~3wgw~~]] ~~is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA].~~	+	*[[Proliferating Cell Nuclear Antigen\|Proliferating Cell Nuclear Antigen]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
-	[[Category: Hashimoto, H.]]	+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Hashimoto, H]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
	[[Category: Dna binding protein-inhibitor complex]]		[[Category: Dna binding protein-inhibitor complex]]

OCA at 11:27, 5 February 2014

OCA — Wed, 05 Feb 2014 11:27:39 GMT

←Older revision		Revision as of 11:27, 5 February 2014
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3wgw\| PDB=3wgw \| SCENE= }}
		+	===Structure of PCNA bound to a small molecule inhibitor===

-	~~The entry 3wgw~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/PCNA_HUMAN PCNA_HUMAN]] Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.<ref>PMID:19443450</ref> <ref>PMID:18719106</ref>

-	~~Authors~~: ~~Hashimoto, H~~.	+	==About this Structure==
		+	[[3wgw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGW OCA].

-	~~Description~~: ~~Structure of PCNA bound to a small molecule~~ inhibitor	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Hashimoto, H.]]
		+	[[Category: Dna binding]]
		+	[[Category: Dna binding protein-inhibitor complex]]

OCA at 03:13, 20 September 2013

OCA — Fri, 20 Sep 2013 03:13:47 GMT

←Older revision		Revision as of 03:13, 20 September 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wgw is ON HOLD	+	The entry 3wgw is ON HOLD until Paper Publication

	Authors: Hashimoto, H.		Authors: Hashimoto, H.

-	Description: Structure of PCNA	+	Description: Structure of PCNA bound to a small molecule inhibitor

OCA: Protected "3wgw" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2013 07:33:52 GMT

Protected "3wgw" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:33, 21 August 2013

OCA: New page: '''Unreleased structure''' The entry 3wgw is ON HOLD Authors: Hashimoto, H. Description: Structure of PCNA

OCA — Wed, 21 Aug 2013 07:33:51 GMT

New page: '''Unreleased structure''' The entry 3wgw is ON HOLD Authors: Hashimoto, H. Description: Structure of PCNA

New page

'''Unreleased structure'''

The entry 3wgw is ON HOLD

Authors: Hashimoto, H.

Description: Structure of PCNA