3wjo - Revision history

OCA at 13:12, 8 November 2023

OCA — Wed, 08 Nov 2023 13:12:50 GMT

←Older revision		Revision as of 13:12, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==		==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==
-	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]]' scene=''>	+	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O104:H4_str._2009EL-2071 Escherichia coli O104:H4 str. 2009EL-2071]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Octaprenyl pyrophosphate synthase (OPPs) catalyzes consecutive condensation reactions of one allylic substrate farnesyl pyrophosphate (FPP) and five homoallylic substrate isopentenyl pyrophosphate (IPP) molecules to form a C40 long-chain product OPP, which serves as a side chain of ubiquinone and menaquinone. OPPs belongs to the trans-prenyltransferase class of proteins. The structures of OPPs from Escherichia coli were solved in the apo-form as well as in complexes with IPP and a FPP thio-analog, FsPP, at resolutions of 2.2 to 2.6 A, and revealed the detailed interactions between the ligands and enzyme. At the bottom of the active-site tunnel, M123 and M135 act in concert to form a wall which determines the final chain length. These results represent the first ligand-bound crystal structures of a long-chain trans-prenyltransferase and provide new information on the mechanisms of catalysis and product chain elongation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.
		+
		+	Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms.,Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191<ref>PMID:24895191</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wjo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli O104:H4 str. 2009EL-2071]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen CC]]		[[Category: Chen CC]]

OCA at 10:33, 31 August 2022

OCA — Wed, 31 Aug 2022 10:33:26 GMT

←Older revision		Revision as of 10:33, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==		==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==
-	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]]~~, [[Resolution\|resolution]] 2.45Å~~' scene=''>	+	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3wjo]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
-	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wjk\|3wjk]], [[3wjn\|3wjn]]</div></td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Octaprenyl pyrophosphate synthase (OPPs) catalyzes consecutive condensation reactions of one allylic substrate farnesyl pyrophosphate (FPP) and five homoallylic substrate isopentenyl pyrophosphate (IPP) molecules to form a C40 long-chain product OPP, which serves as a side chain of ubiquinone and menaquinone. OPPs belongs to the trans-prenyltransferase class of proteins. The structures of OPPs from Escherichia coli were solved in the apo-form as well as in complexes with IPP and a FPP thio-analog, FsPP, at resolutions of 2.2 to 2.6 A, and revealed the detailed interactions between the ligands and enzyme. At the bottom of the active-site tunnel, M123 and M135 act in concert to form a wall which determines the final chain length. These results represent the first ligand-bound crystal structures of a long-chain trans-prenyltransferase and provide new information on the mechanisms of catalysis and product chain elongation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.
-
-	Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms.,Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191<ref>PMID:24895191</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3wjo" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Chen CC]]
-	[[Category: Feng, X]]	+	[[Category: Feng X]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Guo RT]]
-	[[Category: Han, X]]	+	[[Category: Han X]]
-	[[Category: Huang~~, C H~~]]	+	[[Category: Huang CH]]
-	[[Category: Ko~~, T P~~]]	+	[[Category: Ko TP]]
-	[[Category: Kuo~~, C J~~]]	+	[[Category: Kuo CJ]]
-	[[Category: Liang~~, P H~~]]	+	[[Category: Liang PH]]
-	[[Category: Ma~~, Y H~~]]	+	[[Category: Ma YH]]
-	[[Category: Oldfield, E]]	+	[[Category: Oldfield E]]
-	[[Category: Zheng, Y]]	+	[[Category: Zheng Y]]
-	[[Category: Zhu, Z]]	+	[[Category: Zhu Z]]
-	~~[[Category: Prenyltransferase]]~~	+
-	~~[[Category: Product chain length]]~~	+
-	~~[[Category: Site-directed mutagenesis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 04:58, 25 August 2022

OCA — Thu, 25 Aug 2022 04:58:06 GMT

←Older revision		Revision as of 04:58, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Eco1e Eco1e]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wjk\|3wjk]], [[3wjn\|3wjn]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wjk\|3wjk]], [[3wjn\|3wjn]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O3O_22640 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133853 ECO1E])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Eco1e]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]

OCA at 05:39, 3 August 2022

OCA — Wed, 03 Aug 2022 05:39:01 GMT

←Older revision		Revision as of 05:39, 3 August 2022
Line 1:		Line 1:

	==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==		==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==
-	<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='3wjo' size='340' side='right'caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eco1e Eco1e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco1e Eco1e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wjk\|3wjk]], [[3wjn\|3wjn]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O3O_22640 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133853 ECO1E])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O3O_22640 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133853 ECO1E])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [~~http~~://pdbe.org/3wjo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [https://pdbe.org/3wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [https://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Eco1e]]		[[Category: Eco1e]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Feng, X]]		[[Category: Feng, X]]

OCA at 09:41, 21 February 2019

OCA — Thu, 21 Feb 2019 09:41:24 GMT

←Older revision		Revision as of 09:41, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco1e Eco1e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O3O_22640 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1133853 ECO1E])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://pdbe.org/3wjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://pdbe.org/3wjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eco1e]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Feng, X]]		[[Category: Feng, X]]

OCA at 08:50, 11 August 2016

OCA — Thu, 11 Aug 2016 08:50:56 GMT

←Older revision		Revision as of 08:50, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==		==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==
	<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://pdbe.org/3wjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wjo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wjo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:01, 4 January 2015

OCA — Sun, 04 Jan 2015 11:01:54 GMT

←Older revision		Revision as of 11:01, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chen, C C.]]	+	[[Category: Chen, C C]]
-	[[Category: Feng, X.]]	+	[[Category: Feng, X]]
-	[[Category: Guo, R T.]]	+	[[Category: Guo, R T]]
-	[[Category: Han, X.]]	+	[[Category: Han, X]]
-	[[Category: Huang, C H.]]	+	[[Category: Huang, C H]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Kuo, C J.]]	+	[[Category: Kuo, C J]]
-	[[Category: Liang, P H.]]	+	[[Category: Liang, P H]]
-	[[Category: Ma, Y H.]]	+	[[Category: Ma, Y H]]
-	[[Category: Oldfield, E.]]	+	[[Category: Oldfield, E]]
-	[[Category: Zheng, Y.]]	+	[[Category: Zheng, Y]]
-	[[Category: Zhu, Z.]]	+	[[Category: Zhu, Z]]
	[[Category: Prenyltransferase]]		[[Category: Prenyltransferase]]
	[[Category: Product chain length]]		[[Category: Product chain length]]
	[[Category: Site-directed mutagenesis]]		[[Category: Site-directed mutagenesis]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:51, 18 June 2014

OCA — Wed, 18 Jun 2014 07:51:07 GMT

←Older revision		Revision as of 07:51, 18 June 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)==
		+	<StructureSection load='3wjo' size='340' side='right' caption='[[3wjo]], [[Resolution\|resolution]] 2.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wjo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wjk\|3wjk]], [[3wjn\|3wjn]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjo RCSB], [http://www.ebi.ac.uk/pdbsum/3wjo PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Octaprenyl pyrophosphate synthase (OPPs) catalyzes consecutive condensation reactions of one allylic substrate farnesyl pyrophosphate (FPP) and five homoallylic substrate isopentenyl pyrophosphate (IPP) molecules to form a C40 long-chain product OPP, which serves as a side chain of ubiquinone and menaquinone. OPPs belongs to the trans-prenyltransferase class of proteins. The structures of OPPs from Escherichia coli were solved in the apo-form as well as in complexes with IPP and a FPP thio-analog, FsPP, at resolutions of 2.2 to 2.6 A, and revealed the detailed interactions between the ligands and enzyme. At the bottom of the active-site tunnel, M123 and M135 act in concert to form a wall which determines the final chain length. These results represent the first ligand-bound crystal structures of a long-chain trans-prenyltransferase and provide new information on the mechanisms of catalysis and product chain elongation. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc.

-	~~The entry 3wjo is ON HOLD until Paper Publication~~	+	Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from escherichia coli reveal the catalytic and chain-length determining mechanisms.,Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, Liang PH, Guo RT, Ma Y Proteins. 2014 Jun 4. doi: 10.1002/prot.24618. PMID:24895191<ref>PMID:24895191</ref>

-	~~Authors: Han~~, X., Chen, C.C., ~~Kuo~~, ~~C.J~~., Huang, C.H.~~, Zheng, Y.,~~ Ko, T.P., ~~Zhu, Z~~.~~, Feng, X., Oldfield, E.,~~ Liang, P.H., ~~Guo, R.T~~.~~, Ma~~, Y.H.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, C C.]]
		+	[[Category: Feng, X.]]
		+	[[Category: Guo, R T.]]
		+	[[Category: Han, X.]]
		+	[[Category: Huang, C H.]]
		+	[[Category: Ko, T P.]]
		+	[[Category: Kuo, C J.]]
		+	[[Category: Liang, P H.]]
		+	[[Category: Ma, Y H.]]
		+	[[Category: Oldfield, E.]]
		+	[[Category: Zheng, Y.]]
		+	[[Category: Zhu, Z.]]
		+	[[Category: Prenyltransferase]]
		+	[[Category: Product chain length]]
		+	[[Category: Site-directed mutagenesis]]
		+	[[Category: Transferase]]

OCA at 08:42, 18 December 2013

OCA — Wed, 18 Dec 2013 08:42:47 GMT

←Older revision		Revision as of 08:42, 18 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wjo is ON HOLD	+	The entry 3wjo is ON HOLD until Paper Publication

	Authors: Han, X., Chen, C.C., Kuo, C.J., Huang, C.H., Zheng, Y., Ko, T.P., Zhu, Z., Feng, X., Oldfield, E., Liang, P.H., Guo, R.T., Ma, Y.H.		Authors: Han, X., Chen, C.C., Kuo, C.J., Huang, C.H., Zheng, Y., Ko, T.P., Zhu, Z., Feng, X., Oldfield, E., Liang, P.H., Guo, R.T., Ma, Y.H.

	Description: Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)		Description: Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)

OCA at 06:26, 4 December 2013

OCA — Wed, 04 Dec 2013 06:26:38 GMT

←Older revision		Revision as of 06:26, 4 December 2013
Line 3:		Line 3:
	The entry 3wjo is ON HOLD		The entry 3wjo is ON HOLD

-	Authors: Han, X, Chen, C. C. , Kuo, C. J. , Huang, C. H, Zheng Y., Ko, T. P , Zhu, Z., Feng, X., Oldfield, E , Liang, P.H, Guo, R.T., Ma, Y, H.	+	Authors: Han, X., Chen, C.C., Kuo, C.J., Huang, C.H., Zheng, Y., Ko, T.P., Zhu, Z., Feng, X., Oldfield, E., Liang, P.H., Guo, R.T., Ma, Y.H.

	Description: Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)		Description: Crystal structure of Octaprenyl Pyrophosphate synthase from Escherichia coli with isopentenyl pyrophosphate (IPP)