3wnk - Revision history

OCA at 08:46, 20 March 2024

OCA — Wed, 20 Mar 2024 08:46:04 GMT

←Older revision		Revision as of 08:46, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3wnk' size='340' side='right'caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3wnk' size='340' side='right'caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_24 Atcc 24~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]], [[3wnp\|3wnp]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [https://pdbe.org/3wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [https://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [https://pdbe.org/3wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [https://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CTA1_BACCI CTA1_BACCI]~~] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>	+	[https://www.uniprot.org/uniprot/CTA1_NIACI CTA1_NIACI] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase belongs to the glycoside hydrolase family 66 and catalyzes an intramolecular transglucosylation reaction that produces cycloisomaltooligosaccharides from dextran. The crystal structure of the core fragment from Ser-39 to Met-738 of B. circulans T-3040 cycloisomaltooligosaccharide glucanotransferase, devoid of its N-terminal signal peptide and C-terminal nonconserved regions, was determined. The structural model contained one catalytic (beta/alpha)8-barrel domain and three beta-domains. Domain N with an immunoglobulin-like beta-sandwich fold was attached to the N terminus; domain C with a Greek key beta-sandwich fold was located at the C terminus, and a carbohydrate-binding module family 35 (CBM35) beta-jellyroll domain B was inserted between the 7th beta-strand and the 7th alpha-helix of the catalytic domain A. The structures of the inactive catalytic nucleophile mutant enzyme complexed with isomaltohexaose, isomaltoheptaose, isomaltooctaose, and cycloisomaltooctaose revealed that the ligands bound in the catalytic cleft and the sugar-binding site of CBM35. Of these, isomaltooctaose bound in the catalytic site extended to the second sugar-binding site of CBM35, which acted as subsite -8, representing the enzyme.substrate complex when the enzyme produces cycloisomaltooctaose. The isomaltoheptaose and cycloisomaltooctaose bound in the catalytic cleft with a circular structure around Met-310, representing the enzyme.product complex. These structures collectively indicated that CBM35 functions in determining the size of the product, causing the predominant production of cycloisomaltooctaose by the enzyme. The canonical sugar-binding site of CBM35 bound the mid-part of isomaltooligosaccharides, indicating that the original function involved substrate binding required for efficient catalysis.	+
-		+
-	Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.,Suzuki N, Fujimoto Z, Kim YM, Momma M, Kishine N, Suzuki R, Suzuki S, Kitamura S, Kobayashi M, Kimura A, Funane K J Biol Chem. 2014 Apr 25;289(17):12040-51. doi: 10.1074/jbc.M114.547992. Epub, 2014 Mar 10. PMID:24616103<ref>PMID:24616103</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3wnk" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 24]]
-	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fujimoto, Z]]	+	[[Category: Niallia circulans]]
-	[[Category: Funane, K]]	+	[[Category: Fujimoto Z]]
-	[[Category: Kim~~, Y M~~]]	+	[[Category: Funane K]]
-	[[Category: Kimura, A]]	+	[[Category: Kim YM]]
-	[[Category: Kishine, N]]	+	[[Category: Kimura A]]
-	[[Category: Kobayashi, M]]	+	[[Category: Kishine N]]
-	[[Category: Momma, M]]	+	[[Category: Kobayashi M]]
-	[[Category: Suzuki, N]]	+	[[Category: Momma M]]
-	[[Category: Suzuki, R]]	+	[[Category: Suzuki N]]
-	~~[[Category: 6-glucan]]~~	+	[[Category: Suzuki R]]
-	~~[[Category: Alpha-1]]~~	+
-	~~[[Category: Beta-jelly roll]]~~	+
-	~~[[Category: C2 type immunoglobulin fold]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Greek key]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:43, 3 August 2022

OCA — Wed, 03 Aug 2022 05:43:06 GMT

←Older revision		Revision as of 05:43, 3 August 2022
Line 1:		Line 1:

	==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==		==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==
-	<StructureSection load='3wnk' size='340' side='right' caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3wnk' size='340' side='right'caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WNK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]], [[3wnp\|3wnp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]], [[3wnp\|3wnp]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [~~http~~://pdbe.org/3wnk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [https://pdbe.org/3wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [https://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>	+	[[https://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	[[Category: Atcc 24]]		[[Category: Atcc 24]]
	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]		[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
		+	[[Category: Large Structures]]
	[[Category: Fujimoto, Z]]		[[Category: Fujimoto, Z]]
	[[Category: Funane, K]]		[[Category: Funane, K]]

OCA at 19:50, 15 December 2016

OCA — Thu, 15 Dec 2016 19:50:30 GMT

←Older revision		Revision as of 19:50, 15 December 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==		==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==
	<StructureSection load='3wnk' size='340' side='right' caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3wnk' size='340' side='right' caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://pdbe.org/3wnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wnk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:21, 4 March 2015

OCA — Wed, 04 Mar 2015 11:21:55 GMT

←Older revision		Revision as of 11:21, 4 March 2015
Line 13:		Line 13:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase ~~(BcCITase) catalyses~~ an intramolecular transglucosylation reaction ~~and~~ produces cycloisomaltooligosaccharides from dextran. ~~BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by~~ the ~~sitting~~-~~drop vapour~~-~~diffusion method~~. ~~The crystal~~ of ~~BcCITase bearing an~~ N-terminal ~~His~~(6) ~~tag diffracted~~ to a ~~resolution of 2.3 A~~ and ~~belonged to space group P3~~(1)~~21, containing a single molecule in~~ the ~~asymmetric unit~~. The ~~crystal~~ of ~~BcCITase bearing a C~~-~~terminal His6 tag diffracted~~ to ~~a resolution~~ of 1.~~9 A~~ and ~~belonged to space group P2(1)2(1)2(1)~~, ~~containing two molecules~~ in the ~~asymmetric unit~~.	+	Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase belongs to the glycoside hydrolase family 66 and catalyzes an intramolecular transglucosylation reaction that produces cycloisomaltooligosaccharides from dextran. The crystal structure of the core fragment from Ser-39 to Met-738 of B. circulans T-3040 cycloisomaltooligosaccharide glucanotransferase, devoid of its N-terminal signal peptide and C-terminal nonconserved regions, was determined. The structural model contained one catalytic (beta/alpha)8-barrel domain and three beta-domains. Domain N with an immunoglobulin-like beta-sandwich fold was attached to the N terminus; domain C with a Greek key beta-sandwich fold was located at the C terminus, and a carbohydrate-binding module family 35 (CBM35) beta-jellyroll domain B was inserted between the 7th beta-strand and the 7th alpha-helix of the catalytic domain A. The structures of the inactive catalytic nucleophile mutant enzyme complexed with isomaltohexaose, isomaltoheptaose, isomaltooctaose, and cycloisomaltooctaose revealed that the ligands bound in the catalytic cleft and the sugar-binding site of CBM35. Of these, isomaltooctaose bound in the catalytic site extended to the second sugar-binding site of CBM35, which acted as subsite -8, representing the enzyme.substrate complex when the enzyme produces cycloisomaltooctaose. The isomaltoheptaose and cycloisomaltooctaose bound in the catalytic cleft with a circular structure around Met-310, representing the enzyme.product complex. These structures collectively indicated that CBM35 functions in determining the size of the product, causing the predominant production of cycloisomaltooctaose by the enzyme. The canonical sugar-binding site of CBM35 bound the mid-part of isomaltooligosaccharides, indicating that the original function involved substrate binding required for efficient catalysis.

-	~~Crystallization and preliminary X-ray crystallographic analysis~~ of ~~cycloisomaltooligosaccharide glucanotransferase from~~ Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, ~~Fujimoto Z~~, Kobayashi M, Kimura A, Funane K ~~Acta Crystallogr Sect F Struct~~ Biol ~~Cryst Commun~~. ~~2013 Aug~~;69(~~Pt 8~~):~~946~~-9. doi:, 10.~~1107~~/~~S174430911301991X~~. Epub ~~2013 Jul 27~~. PMID:~~23908050~~<ref>PMID:~~23908050~~</ref>	+	Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.,Suzuki N, Fujimoto Z, Kim YM, Momma M, Kishine N, Suzuki R, Suzuki S, Kitamura S, Kobayashi M, Kimura A, Funane K J Biol Chem. 2014 Apr 25;289(17):12040-51. doi: 10.1074/jbc.M114.547992. Epub, 2014 Mar 10. PMID:24616103<ref>PMID:24616103</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 07:40, 25 December 2014

OCA — Thu, 25 Dec 2014 07:40:09 GMT

←Older revision		Revision as of 07:40, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:25, 21 December 2014

OCA — Sun, 21 Dec 2014 18:25:30 GMT

←Older revision		Revision as of 18:25, 21 December 2014
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-	~~{{STRUCTURE_3wnk\| PDB=3wnk \| SCENE= }}~~	+	==Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase==
-	===Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase===	+	<StructureSection load='3wnk' size='340' side='right' caption='[[3wnk]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23908050}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wnk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WNK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]], [[3wnp\|3wnp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnk RCSB], [http://www.ebi.ac.uk/pdbsum/3wnk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His(6) tag diffracted to a resolution of 2.3 A and belonged to space group P3(1)21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 A and belonged to space group P2(1)2(1)2(1), containing two molecules in the asymmetric unit.

-	~~==Function==~~	+	Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050<ref>PMID:23908050</ref>
-	~~[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces~~ cycloisomaltooligosaccharide from ~~dextran containing 7, 8 or 9 glucose units~~. ~~The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and~~, ~~without activity toward (1->4)-alpha-D-glucans~~, ~~such as amylose. It also has no activity on oligosaccharides~~, ~~such as amylopectin and pullulan~~, ~~containing~~ (~~1->6~~)-~~alpha-D-glucosidic linkages at branch points~~.<ref>PMID:~~7515357~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3wnk]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3WNK OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023908050</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 24]]
	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]		[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
-	[[Category: Fujimoto, Z.]]	+	[[Category: Fujimoto, Z]]
-	[[Category: Funane, K.]]	+	[[Category: Funane, K]]
-	[[Category: Kim, Y M.]]	+	[[Category: Kim, Y M]]
-	[[Category: Kimura, A.]]	+	[[Category: Kimura, A]]
-	[[Category: Kishine, N.]]	+	[[Category: Kishine, N]]
-	[[Category: Kobayashi, M.]]	+	[[Category: Kobayashi, M]]
-	[[Category: Momma, M.]]	+	[[Category: Momma, M]]
-	[[Category: Suzuki, N.]]	+	[[Category: Suzuki, N]]
-	[[Category: Suzuki, R.]]	+	[[Category: Suzuki, R]]
	[[Category: 6-glucan]]		[[Category: 6-glucan]]
	[[Category: Alpha-1]]		[[Category: Alpha-1]]

OCA at 11:05, 5 February 2014

OCA — Wed, 05 Feb 2014 11:05:39 GMT

←Older revision		Revision as of 11:05, 5 February 2014
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3wnk\| PDB=3wnk \| SCENE= }}
		+	===Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase===
		+	{{ABSTRACT_PUBMED_23908050}}

-	The ~~entry 3wnk~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>

-	~~Authors~~: ~~Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R~~.~~, Kobayashi, M~~.~~, Kimura, A~~.~~, Funane, K~~.	+	==About this Structure==
		+	[[3wnk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNK OCA].

-	~~Description~~: ~~Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide~~ glucanotransferase	+	==Reference==
		+	<ref group="xtra">PMID:023908050</ref><references group="xtra"/><references/>
		+	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
		+	[[Category: Fujimoto, Z.]]
		+	[[Category: Funane, K.]]
		+	[[Category: Kim, Y M.]]
		+	[[Category: Kimura, A.]]
		+	[[Category: Kishine, N.]]
		+	[[Category: Kobayashi, M.]]
		+	[[Category: Momma, M.]]
		+	[[Category: Suzuki, N.]]
		+	[[Category: Suzuki, R.]]
		+	[[Category: 6-glucan]]
		+	[[Category: Alpha-1]]
		+	[[Category: Beta-jelly roll]]
		+	[[Category: C2 type immunoglobulin fold]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Greek key]]
		+	[[Category: Transferase]]

OCA at 07:22, 15 January 2014

OCA — Wed, 15 Jan 2014 07:22:52 GMT

←Older revision		Revision as of 07:22, 15 January 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wnk is ON HOLD	+	The entry 3wnk is ON HOLD until Paper Publication

	Authors: Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Kobayashi, M., Kimura, A., Funane, K.		Authors: Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Kobayashi, M., Kimura, A., Funane, K.

	Description: Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase		Description: Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase

OCA: Protected "3wnk" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2013 08:44:47 GMT

Protected "3wnk" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:44, 18 December 2013

OCA: New page: '''Unreleased structure''' The entry 3wnk is ON HOLD Authors: Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Kobayashi, M., Kimura, A., Funane, K. Description...

OCA — Wed, 18 Dec 2013 08:44:46 GMT

New page: '''Unreleased structure''' The entry 3wnk is ON HOLD Authors: Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Kobayashi, M., Kimura, A., Funane, K. Description...

New page

'''Unreleased structure'''

The entry 3wnk is ON HOLD

Authors: Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Kobayashi, M., Kimura, A., Funane, K.

Description: Crystal Structure of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase