3wnp - Revision history

OCA at 13:17, 8 November 2023

OCA — Wed, 08 Nov 2023 13:17:38 GMT

←Older revision		Revision as of 13:17, 8 November 2023
Line 1:		Line 1:

	==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==		==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
-	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]]' scene=''>	+	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CTA1_NIACI CTA1_NIACI] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cycloisomaltooligosaccharide glucanotransferase (CITase) is a member of the glycoside hydrolase family 66, and it produces cycloisomaltooligosaccharides (CIs). Small CIs (CI-7-9) and large CIs (CI->/=10) are designated as oligosaccharide-type CIs (oligo-CIs) and megalosaccharide-type CIs (megalo-CIs), respectively. CITase from Bacillus circulans T-3040 (BcCITase) produces mainly CI-8 with little megalo-CIs. It has two family 35 carbohydrate-binding modules (BcCBM35-1 and BcCBM35-2). BcCBM35-1 is inserted in a catalytic domain of BcCITase, and BcCBM35-2 is located at the C-terminal region. Our previous studies suggested that BcCBM35-1 has two substrate-binding sites (B-1 and B-2). We implemented site-directed mutagenesis of BcCITase to explore the preference for product size on the basis of the 3D structure of BcCITase. Mutational studies provided evidence that B-1 and B-2 contribute to recruiting substrate and maintaining product size, respectively. A mutant (mutant-R) with four mutations (F268V, D469Y, A513V and Y515S) produced three times as much megalo-CIs (CI-10-12) and 1.5 times as much total CIs (CI-7-12) as compared with the wild-type BcCITase. The 3D structure of the substrate-enzyme complex of mutant-R suggested that the modified product size specificity was attributable to the construction of novel substrate-binding sites in the B-2 site of BcCBM35-1, and reactivity was improved by mutation on subsite -3 on the catalytic domain.
		+
		+	Molecular engineering of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040: structural determinants for the reaction product size and reactivity.,Suzuki R, Suzuki N, Fujimoto Z, Momma M, Kimura K, Kitamura S, Kimura A, Funane K Biochem J. 2015 Feb 4. PMID:25649478<ref>PMID:25649478</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3wnp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Niallia circulans]]
	[[Category: Fujimoto Z]]		[[Category: Fujimoto Z]]
	[[Category: Funane K]]		[[Category: Funane K]]

OCA at 10:34, 31 August 2022

OCA — Wed, 31 Aug 2022 10:34:49 GMT

←Older revision		Revision as of 10:34, 31 August 2022
Line 1:		Line 1:

	==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==		==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
-	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]]~~, [[Resolution\|resolution]] 2.80Å~~' scene=''>	+	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[3wnp]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</div></td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cycloisomaltooligosaccharide glucanotransferase (CITase) is a member of the glycoside hydrolase family 66, and it produces cycloisomaltooligosaccharides (CIs). Small CIs (CI-7-9) and large CIs (CI->/=10) are designated as oligosaccharide-type CIs (oligo-CIs) and megalosaccharide-type CIs (megalo-CIs), respectively. CITase from Bacillus circulans T-3040 (BcCITase) produces mainly CI-8 with little megalo-CIs. It has two family 35 carbohydrate-binding modules (BcCBM35-1 and BcCBM35-2). BcCBM35-1 is inserted in a catalytic domain of BcCITase, and BcCBM35-2 is located at the C-terminal region. Our previous studies suggested that BcCBM35-1 has two substrate-binding sites (B-1 and B-2). We implemented site-directed mutagenesis of BcCITase to explore the preference for product size on the basis of the 3D structure of BcCITase. Mutational studies provided evidence that B-1 and B-2 contribute to recruiting substrate and maintaining product size, respectively. A mutant (mutant-R) with four mutations (F268V, D469Y, A513V and Y515S) produced three times as much megalo-CIs (CI-10-12) and 1.5 times as much total CIs (CI-7-12) as compared with the wild-type BcCITase. The 3D structure of the substrate-enzyme complex of mutant-R suggested that the modified product size specificity was attributable to the construction of novel substrate-binding sites in the B-2 site of BcCBM35-1, and reactivity was improved by mutation on subsite -3 on the catalytic domain.
-
-	Molecular engineering of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040: structural determinants for the reaction product size and reactivity.,Suzuki R, Suzuki N, Fujimoto Z, Momma M, Kimura K, Kitamura S, Kimura A, Funane K Biochem J. 2015 Feb 4. PMID:25649478<ref>PMID:25649478</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3wnp" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fujimoto, Z]]	+	[[Category: Fujimoto Z]]
-	[[Category: Funane, K]]	+	[[Category: Funane K]]
-	[[Category: Kimura, A]]	+	[[Category: Kimura A]]
-	[[Category: Kimura, K]]	+	[[Category: Kimura K]]
-	[[Category: Kitamura, S]]	+	[[Category: Kitamura S]]
-	[[Category: Momma, M]]	+	[[Category: Momma M]]
-	[[Category: Suzuki, N]]	+	[[Category: Suzuki N]]
-	[[Category: Suzuki, R]]	+	[[Category: Suzuki R]]
-	~~[[Category: 6-glucan]]~~	+
-	~~[[Category: Alpha-1]]~~	+
-	~~[[Category: Beta-jelly roll]]~~	+
-	~~[[Category: C2 type immunoglobulin fold]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Greek key]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 04:58, 25 August 2022

OCA — Thu, 25 Aug 2022 04:58:45 GMT

←Older revision		Revision as of 04:58, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Atcc_24 Atcc 24]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 24]]
	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]		[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 05:43, 3 August 2022

OCA — Wed, 03 Aug 2022 05:43:20 GMT

←Older revision		Revision as of 05:43, 3 August 2022
Line 1:		Line 1:

	==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==		==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
-	<StructureSection load='3wnp' size='340' side='right' caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3wnp' size='340' side='right'caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WNP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [~~http~~://pdbe.org/3wnp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [https://pdbe.org/3wnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [https://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>	+	[[https://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	[[Category: Atcc 24]]		[[Category: Atcc 24]]
	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]		[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
		+	[[Category: Large Structures]]
	[[Category: Fujimoto, Z]]		[[Category: Fujimoto, Z]]
	[[Category: Funane, K]]		[[Category: Funane, K]]

OCA at 19:57, 15 December 2016

OCA — Thu, 15 Dec 2016 19:57:13 GMT

←Older revision		Revision as of 19:57, 15 December 2016
Line 1:		Line 1:
		+
	==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==		==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
	<StructureSection load='3wnp' size='340' side='right' caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3wnp' size='340' side='right' caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://pdbe.org/3wnp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wnp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wnp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:28, 4 March 2015

OCA — Wed, 04 Mar 2015 11:28:05 GMT

←Older revision		Revision as of 11:28, 4 March 2015
Line 13:		Line 13:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	Bacillus circulans T-3040 ~~cycloisomaltooligosaccharide glucanotransferase~~ (BcCITase) ~~catalyses an intramolecular transglucosylation reaction and~~ produces ~~cycloisomaltooligosaccharides from dextran~~. ~~BcCITase was overexpressed in Escherichia coli in~~ two ~~different forms and crystallized by the sitting~~-~~drop vapour~~-~~diffusion method~~. ~~The crystal~~ of BcCITase ~~bearing an N~~-terminal ~~His~~(6) ~~tag diffracted~~ to ~~a resolution~~ of 2.3 A and ~~belonged to space group P3~~(1)~~21, containing a single molecule in~~ the ~~asymmetric unit~~. The ~~crystal~~ of ~~BcCITase bearing a C~~-~~terminal His6 tag diffracted to a resolution~~ of ~~1.9 A and belonged~~ to ~~space group P2(1)~~2(1~~)2(1)~~, ~~containing two molecules in~~ the ~~asymmetric unit~~.	+	Cycloisomaltooligosaccharide glucanotransferase (CITase) is a member of the glycoside hydrolase family 66, and it produces cycloisomaltooligosaccharides (CIs). Small CIs (CI-7-9) and large CIs (CI->/=10) are designated as oligosaccharide-type CIs (oligo-CIs) and megalosaccharide-type CIs (megalo-CIs), respectively. CITase from Bacillus circulans T-3040 (BcCITase) produces mainly CI-8 with little megalo-CIs. It has two family 35 carbohydrate-binding modules (BcCBM35-1 and BcCBM35-2). BcCBM35-1 is inserted in a catalytic domain of BcCITase, and BcCBM35-2 is located at the C-terminal region. Our previous studies suggested that BcCBM35-1 has two substrate-binding sites (B-1 and B-2). We implemented site-directed mutagenesis of BcCITase to explore the preference for product size on the basis of the 3D structure of BcCITase. Mutational studies provided evidence that B-1 and B-2 contribute to recruiting substrate and maintaining product size, respectively. A mutant (mutant-R) with four mutations (F268V, D469Y, A513V and Y515S) produced three times as much megalo-CIs (CI-10-12) and 1.5 times as much total CIs (CI-7-12) as compared with the wild-type BcCITase. The 3D structure of the substrate-enzyme complex of mutant-R suggested that the modified product size specificity was attributable to the construction of novel substrate-binding sites in the B-2 site of BcCBM35-1, and reactivity was improved by mutation on subsite -3 on the catalytic domain.

-	~~Crystallization and preliminary X-ray crystallographic analysis~~ of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, ~~Kim YM~~, Momma M, ~~Fujimoto Z~~, ~~Kobayashi M~~, Kimura A, Funane K ~~Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X~~. ~~Epub 2013 Jul 27~~. PMID:~~23908050~~<ref>PMID:~~23908050~~</ref>	+	Molecular engineering of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040: structural determinants for the reaction product size and reactivity.,Suzuki R, Suzuki N, Fujimoto Z, Momma M, Kimura K, Kitamura S, Kimura A, Funane K Biochem J. 2015 Feb 4. PMID:25649478<ref>PMID:25649478</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 01:11, 25 December 2014

OCA — Thu, 25 Dec 2014 01:11:53 GMT

←Older revision		Revision as of 01:11, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:59, 21 December 2014

OCA — Sun, 21 Dec 2014 17:59:13 GMT

←Older revision		Revision as of 17:59, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3wnp\| PDB=3wnp \| SCENE= }}~~	+	==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
-	===D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose===	+	<StructureSection load='3wnp' size='340' side='right' caption='[[3wnp]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23908050}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wnk\|3wnk]], [[3wnl\|3wnl]], [[3wnm\|3wnm]], [[3wnn\|3wnn]], [[3wno\|3wno]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His(6) tag diffracted to a resolution of 2.3 A and belonged to space group P3(1)21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 A and belonged to space group P2(1)2(1)2(1), containing two molecules in the asymmetric unit.

-	~~==Function==~~	+	Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050<ref>PMID:23908050</ref>
-	~~[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces~~ cycloisomaltooligosaccharide from ~~dextran containing 7, 8 or 9 glucose units~~. ~~The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and~~, ~~without activity toward (1->4)-alpha-D-glucans~~, ~~such as amylose. It also has no activity on oligosaccharides~~, ~~such as amylopectin and pullulan~~, ~~containing~~ (~~1->6~~)-~~alpha-D-glucosidic linkages at branch points~~.<ref>PMID:~~7515357~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3wnp]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3WNP OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023908050</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 24]]
	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]		[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
-	[[Category: Fujimoto, Z.]]	+	[[Category: Fujimoto, Z]]
-	[[Category: Funane, K.]]	+	[[Category: Funane, K]]
-	[[Category: Kimura, A.]]	+	[[Category: Kimura, A]]
-	[[Category: Kimura, K.]]	+	[[Category: Kimura, K]]
-	[[Category: Kitamura, S.]]	+	[[Category: Kitamura, S]]
-	[[Category: Momma, M.]]	+	[[Category: Momma, M]]
-	[[Category: Suzuki, N.]]	+	[[Category: Suzuki, N]]
-	[[Category: Suzuki, R.]]	+	[[Category: Suzuki, R]]
	[[Category: 6-glucan]]		[[Category: 6-glucan]]
	[[Category: Alpha-1]]		[[Category: Alpha-1]]

OCA at 11:13, 5 February 2014

OCA — Wed, 05 Feb 2014 11:13:09 GMT

←Older revision		Revision as of 11:13, 5 February 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3wnp\| PDB=3wnp \| SCENE= }}
		+	===D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose===
		+	{{ABSTRACT_PUBMED_23908050}}

-	The ~~entry 3wnp~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>

-	~~Authors~~: ~~Suzuki, R., Suzuki, N., Fujimoto, Z., Momma, M., Kimura, K~~.~~, Kitamura, S~~.~~, Kimura, A~~.~~, Funane, K~~.	+	==About this Structure==
		+	[[3wnp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA].

-	~~Description~~: ~~D308A~~, ~~F268V~~, ~~D469Y~~, ~~A513V~~, ~~and Y515S quintuple mutant of Bacillus circulans T~~-~~3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose~~	+	==Reference==
		+	<ref group="xtra">PMID:023908050</ref><references group="xtra"/><references/>
		+	[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
		+	[[Category: Fujimoto, Z.]]
		+	[[Category: Funane, K.]]
		+	[[Category: Kimura, A.]]
		+	[[Category: Kimura, K.]]
		+	[[Category: Kitamura, S.]]
		+	[[Category: Momma, M.]]
		+	[[Category: Suzuki, N.]]
		+	[[Category: Suzuki, R.]]
		+	[[Category: 6-glucan]]
		+	[[Category: Alpha-1]]
		+	[[Category: Beta-jelly roll]]
		+	[[Category: C2 type immunoglobulin fold]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Greek key]]
		+	[[Category: Transferase]]

OCA at 06:38, 22 January 2014

OCA — Wed, 22 Jan 2014 06:38:04 GMT

←Older revision		Revision as of 06:38, 22 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wnp is ON HOLD	+	The entry 3wnp is ON HOLD until Paper Publication

	Authors: Suzuki, R., Suzuki, N., Fujimoto, Z., Momma, M., Kimura, K., Kitamura, S., Kimura, A., Funane, K.		Authors: Suzuki, R., Suzuki, N., Fujimoto, Z., Momma, M., Kimura, K., Kitamura, S., Kimura, A., Funane, K.

	Description: D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose		Description: D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose