3wqk - Revision history

OCA at 08:46, 20 March 2024

OCA — Wed, 20 Mar 2024 08:46:24 GMT

←Older revision		Revision as of 08:46, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3wqk' size='340' side='right'caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3wqk' size='340' side='right'caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Rv3378c, MT3488 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [https://pdbe.org/3wqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [https://www.ebi.ac.uk/pdbsum/3wqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [https://pdbe.org/3wqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [https://www.ebi.ac.uk/pdbsum/3wqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TUBOL_MYCTU TUBOL_MYCTU~~]~~] Catalyzes~~ the PP-removal from ~~tuberculosinyl diphosphate~~ to produce both ~~tubercilosinol~~ and isotuberculosinol. ~~Can~~ also use labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro.	+	[https://www.uniprot.org/uniprot/TUBAT_MYCTU TUBAT_MYCTU] Tuberculosinyl transferase that catalyzes the condensation of adenosine and tuberculosinyl diphosphate (TbPP) to generate 1-tuberculosinyladenosine (1-TbAd), which acts as an antiacid that directly protects M.tuberculosis from acid pH and physically remodels M.tuberculosis phagolysosomes (PubMed:24516143, PubMed:31427817). In addition, acts as a phosphatase that catalyzes the diphosphate-removal from TbPP to produce both tuberculosinol (TOH) and isotuberculosinol (iso-TOH) (PubMed:21228491, PubMed:24475925). Has broad substrate specificity, and can also use the 3 labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro (PubMed:21290071).<ref>PMID:21228491</ref> <ref>PMID:21290071</ref> <ref>PMID:24475925</ref> <ref>PMID:24516143</ref> <ref>PMID:31427817</ref>
-	<~~div style="background-color~~:~~#fffaf0;"~~>	+
-	~~== Publication Abstract from PubMed ==~~	+
-	We have obtained the structure of the bacterial diterpene synthase, tuberculosinol/iso-tuberculosinol synthase (Rv3378c) from Mycobacterium tuberculosis , a target for anti-infective therapies that block virulence factor formation. This phosphatase adopts the same fold as found in the Z- or cis-prenyltransferases. We also obtained structures containing the tuberculosinyl diphosphate substrate together with one bisphosphonate inhibitor-bound structure. These structures together with the results of site-directed mutagenesis suggest an unusual mechanism of action involving two Tyr residues. Given the similarity in local and global structure between Rv3378c and the M. tuberculosis cis-decaprenyl diphosphate synthase (DPPS; Rv2361c), the possibility exists for the development of inhibitors that target not only virulence but also cell wall biosynthesis, based in part on the structures reported here.	+
-		+
-	Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis.,Chan HC, Feng X, Ko TP, Huang CH, Hu Y, Zheng Y, Bogue S, Nakano C, Hoshino T, Zhang L, Lv P, Liu W, Crick DC, Liang PH, Wang AH, Oldfield E, Guo RT J Am Chem Soc. 2014 Feb 5. PMID:~~24475925~~<ref>PMID:24475925</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 3wqk" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 15:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bogue, S]]	+	[[Category: Mycobacterium tuberculosis]]
-	[[Category: Chan~~, H C~~]]	+	[[Category: Bogue S]]
-	[[Category: Crick~~, D C~~]]	+	[[Category: Chan HC]]
-	[[Category: Feng, X]]	+	[[Category: Crick DC]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Feng X]]
-	[[Category: Hoshino, T]]	+	[[Category: Guo RT]]
-	[[Category: Hu, Y]]	+	[[Category: Hoshino T]]
-	[[Category: Huang~~, C H~~]]	+	[[Category: Hu Y]]
-	[[Category: Ko~~, T P~~]]	+	[[Category: Huang CH]]
-	[[Category: Liang~~, P H~~]]	+	[[Category: Ko TP]]
-	[[Category: Liu, W]]	+	[[Category: Liang PH]]
-	[[Category: Lv, P]]	+	[[Category: Liu W]]
-	[[Category: Nakano, C]]	+	[[Category: Lv P]]
-	[[Category: Oldfield, E]]	+	[[Category: Nakano C]]
-	[[Category: Wang~~, A H~~]]	+	[[Category: Oldfield E]]
-	[[Category: Zhang, L]]	+	[[Category: Wang AH]]
-	[[Category: Zheng, Y]]	+	[[Category: Zhang L]]
-	~~[[Category: Diterpene synthase]]~~	+	[[Category: Zheng Y]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphatase~~]]	+

OCA at 05:46, 3 August 2022

OCA — Wed, 03 Aug 2022 05:46:25 GMT

←Older revision		Revision as of 05:46, 3 August 2022
Line 1:		Line 1:

	==Crystal structure of Rv3378c with PO4==		==Crystal structure of Rv3378c with PO4==
-	<StructureSection load='3wqk' size='340' side='right' caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3wqk' size='340' side='right'caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [~~http~~://pdbe.org/3wqk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wqk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [https://pdbe.org/3wqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [https://www.ebi.ac.uk/pdbsum/3wqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TUBOL_MYCTU TUBOL_MYCTU]] Catalyzes the PP-removal from tuberculosinyl diphosphate to produce both tubercilosinol and isotuberculosinol. Can also use labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro.	+	[[https://www.uniprot.org/uniprot/TUBOL_MYCTU TUBOL_MYCTU]] Catalyzes the PP-removal from tuberculosinyl diphosphate to produce both tubercilosinol and isotuberculosinol. Can also use labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bogue, S]]		[[Category: Bogue, S]]
	[[Category: Chan, H C]]		[[Category: Chan, H C]]

OCA at 11:37, 4 August 2016

OCA — Thu, 04 Aug 2016 11:37:04 GMT

←Older revision		Revision as of 11:37, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Rv3378c with PO4==		==Crystal structure of Rv3378c with PO4==
	<StructureSection load='3wqk' size='340' side='right' caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3wqk' size='340' side='right' caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [http://www.ebi.ac.uk/pdbsum/3wqk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [http://pdbe.org/3wqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [http://www.ebi.ac.uk/pdbsum/3wqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wqk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wqk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:59, 24 December 2014

OCA — Wed, 24 Dec 2014 13:59:36 GMT

←Older revision		Revision as of 13:59, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQK FirstGlance]. <br>		<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [http://www.ebi.ac.uk/pdbsum/3wqk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [http://www.ebi.ac.uk/pdbsum/3wqk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TUBOL_MYCTU TUBOL_MYCTU]] Catalyzes the PP-removal from tuberculosinyl diphosphate to produce both tubercilosinol and isotuberculosinol. Can also use labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bogue, S.]]	+	[[Category: Bogue, S]]
-	[[Category: Chan, H C.]]	+	[[Category: Chan, H C]]
-	[[Category: Crick, D C.]]	+	[[Category: Crick, D C]]
-	[[Category: Feng, X.]]	+	[[Category: Feng, X]]
-	[[Category: Guo, R T.]]	+	[[Category: Guo, R T]]
-	[[Category: Hoshino, T.]]	+	[[Category: Hoshino, T]]
-	[[Category: Hu, Y.]]	+	[[Category: Hu, Y]]
-	[[Category: Huang, C H.]]	+	[[Category: Huang, C H]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Liang, P H.]]	+	[[Category: Liang, P H]]
-	[[Category: Liu, W.]]	+	[[Category: Liu, W]]
-	[[Category: Lv, P.]]	+	[[Category: Lv, P]]
-	[[Category: Nakano, C.]]	+	[[Category: Nakano, C]]
-	[[Category: Oldfield, E.]]	+	[[Category: Oldfield, E]]
-	[[Category: Wang, A H.]]	+	[[Category: Wang, A H]]
-	[[Category: Zhang, L.]]	+	[[Category: Zhang, L]]
-	[[Category: Zheng, Y.]]	+	[[Category: Zheng, Y]]
	[[Category: Diterpene synthase]]		[[Category: Diterpene synthase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphatase]]		[[Category: Phosphatase]]

OCA at 22:01, 1 October 2014

OCA — Wed, 01 Oct 2014 22:01:39 GMT

←Older revision		Revision as of 22:01, 1 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3wqk\| PDB~~=3wqk \| ~~SCENE~~= }}	+	==Crystal structure of Rv3378c with PO4==
-	===~~Crystal~~ structure ~~of Rv3378c~~ with PO4===	+	<StructureSection load='3wqk' size='340' side='right' caption='[[3wqk]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24475925}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wqk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WQK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kt8\|4kt8]], [[3wql\|3wql]], [[3wqm\|3wqm]], [[3wqn\|3wqn]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3378c, MT3488 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wqk RCSB], [http://www.ebi.ac.uk/pdbsum/3wqk PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have obtained the structure of the bacterial diterpene synthase, tuberculosinol/iso-tuberculosinol synthase (Rv3378c) from Mycobacterium tuberculosis , a target for anti-infective therapies that block virulence factor formation. This phosphatase adopts the same fold as found in the Z- or cis-prenyltransferases. We also obtained structures containing the tuberculosinyl diphosphate substrate together with one bisphosphonate inhibitor-bound structure. These structures together with the results of site-directed mutagenesis suggest an unusual mechanism of action involving two Tyr residues. Given the similarity in local and global structure between Rv3378c and the M. tuberculosis cis-decaprenyl diphosphate synthase (DPPS; Rv2361c), the possibility exists for the development of inhibitors that target not only virulence but also cell wall biosynthesis, based in part on the structures reported here.

-	~~==Function==~~	+	Structure and Inhibition of Tuberculosinol Synthase and Decaprenyl Diphosphate Synthase from Mycobacterium tuberculosis.,Chan HC, Feng X, Ko TP, Huang CH, Hu Y, Zheng Y, Bogue S, Nakano C, Hoshino T, Zhang L, Lv P, Liu W, Crick DC, Liang PH, Wang AH, Oldfield E, Guo RT J Am Chem Soc. 2014 Feb 5. PMID:24475925<ref>PMID:24475925</ref>
-	~~[[http://www.uniprot.org/uniprot/TUBOL_MYCTU TUBOL_MYCTU]] Catalyzes the PP-removal from tuberculosinyl diphosphate to produce both tubercilosinol~~ and ~~isotuberculosinol~~. ~~Can also use labdadienyl diphosphates~~, ~~copalyl diphosphate (CDP)~~, ~~ent-CDP and syn-CDP in vitro~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3wqk]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3WQK OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024475925</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bogue, S.]]		[[Category: Bogue, S.]]
	[[Category: Chan, H C.]]		[[Category: Chan, H C.]]

OCA at 17:29, 19 February 2014

OCA — Wed, 19 Feb 2014 17:29:25 GMT

←Older revision		Revision as of 17:29, 19 February 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_3wqk\| PDB=3wqk \| SCENE= }}
		+	===Crystal structure of Rv3378c with PO4===
		+	{{ABSTRACT_PUBMED_24475925}}

-	~~The entry 3wqk is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/TUBOL_MYCTU TUBOL_MYCTU]] Catalyzes the PP-removal from tuberculosinyl diphosphate to produce both tubercilosinol and isotuberculosinol. Can also use labdadienyl diphosphates, copalyl diphosphate (CDP), ent-CDP and syn-CDP in vitro.

-	~~Authors~~: ~~Chan, H.C., Feng, X., Ko, T.P., Huang, C.H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D., Liang, P.H., Wang, H.J~~.~~, Oldfield, E~~.~~, Guo, R~~.T.	+	==About this Structure==
		+	[[3wqk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQK OCA].

-	~~Description~~: ~~Crystal structure of Rv3378c with PO4~~	+	==Reference==
		+	<ref group="xtra">PMID:024475925</ref><references group="xtra"/><references/>
		+	[[Category: Bogue, S.]]
		+	[[Category: Chan, H C.]]
		+	[[Category: Crick, D C.]]
		+	[[Category: Feng, X.]]
		+	[[Category: Guo, R T.]]
		+	[[Category: Hoshino, T.]]
		+	[[Category: Hu, Y.]]
		+	[[Category: Huang, C H.]]
		+	[[Category: Ko, T P.]]
		+	[[Category: Liang, P H.]]
		+	[[Category: Liu, W.]]
		+	[[Category: Lv, P.]]
		+	[[Category: Nakano, C.]]
		+	[[Category: Oldfield, E.]]
		+	[[Category: Wang, A H.]]
		+	[[Category: Zhang, L.]]
		+	[[Category: Zheng, Y.]]
		+	[[Category: Diterpene synthase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphatase]]

OCA at 04:43, 13 February 2014

OCA — Thu, 13 Feb 2014 04:43:33 GMT

←Older revision		Revision as of 04:43, 13 February 2014
Line 3:		Line 3:
	The entry 3wqk is ON HOLD		The entry 3wqk is ON HOLD

-	Authors: Chan, H.C., Feng,X., Ko,T.P., Huang, C.H., Hu,Y., Zheng,Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D., Liang, P. H., Wang, H. J., Oldfield, E, Guo, R. T.	+	Authors: Chan, H.C., Feng, X., Ko, T.P., Huang, C.H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D., Liang, P.H., Wang, H.J., Oldfield, E., Guo, R.T.

	Description: Crystal structure of Rv3378c with PO4		Description: Crystal structure of Rv3378c with PO4

OCA: Protected "3wqk" [edit=sysop:move=sysop]

OCA — Wed, 05 Feb 2014 09:42:12 GMT

Protected "3wqk" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:42, 5 February 2014

OCA: New page: '''Unreleased structure''' The entry 3wqk is ON HOLD Authors: Chan, H.C., Feng,X., Ko,T.P., Huang, C.H., Hu,Y., Zheng,Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W.,...

OCA — Wed, 05 Feb 2014 09:42:11 GMT

New page: '''Unreleased structure''' The entry 3wqk is ON HOLD Authors: Chan, H.C., Feng,X., Ko,T.P., Huang, C.H., Hu,Y., Zheng,Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W.,...

New page

'''Unreleased structure'''

The entry 3wqk is ON HOLD

Authors: Chan, H.C., Feng,X., Ko,T.P., Huang, C.H., Hu,Y., Zheng,Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D., Liang, P. H., Wang, H. J., Oldfield, E, Guo, R. T.

Description: Crystal structure of Rv3378c with PO4