3wub - Revision history

OCA at 13:29, 8 November 2023

OCA — Wed, 08 Nov 2023 13:29:24 GMT

←Older revision		Revision as of 13:29, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='3wub' size='340' side='right'caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='3wub' size='340' side='right'caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chainia_sp~~. ~~Chainia~~ sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3wue\|3wue]]~~, [[~~3wuf~~\|~~3wuf~~]]~~, [[3wug\|3wug]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>xynAS9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1931 Chainia sp.])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [https://pdbe.org/3wub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [https://www.ebi.ac.uk/pdbsum/3wub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [https://pdbe.org/3wub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [https://www.ebi.ac.uk/pdbsum/3wub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/XYNA_STRSQ XYNA_STRSQ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chainia sp]]
-	[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen~~, C C~~]]	+	[[Category: Streptomyces sp]]
-	[[Category: Gao, J]]	+	[[Category: Chen CC]]
-	[[Category: Guo~~, R T~~]]	+	[[Category: Gao J]]
-	[[Category: Han, X]]	+	[[Category: Guo RT]]
-	[[Category: Huang~~, C H~~]]	+	[[Category: Han X]]
-	[[Category: Ko~~, T P~~]]	+	[[Category: Huang CH]]
-	[[Category: Lv, P]]	+	[[Category: Ko TP]]
-	[[Category: Peng, W]]	+	[[Category: Lv P]]
-	[[Category: Yang~~, Y Y~~]]	+	[[Category: Peng W]]
-	[[Category: Zheng, Y]]	+	[[Category: Yang YY]]
-	~~[[Category: 4-xylanase]]~~	+	[[Category: Zheng Y]]
-	~~[[Category: Beta-1]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Protein rigidity]]~~	+
-	~~[[Category: Thermozyme~~]]	+

OCA at 05:31, 10 August 2022

OCA — Wed, 10 Aug 2022 05:31:45 GMT

←Older revision		Revision as of 05:31, 10 August 2022
Line 1:		Line 1:

	==The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9==		==The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9==
-	<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>	+	<StructureSection load='3wub' size='340' side='right'caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3WUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WUB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [~~http~~://pdbe.org/3wub PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3wub PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [https://pdbe.org/3wub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [https://www.ebi.ac.uk/pdbsum/3wub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Chainia sp]]		[[Category: Chainia sp]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]
	[[Category: Gao, J]]		[[Category: Gao, J]]

OCA at 08:32, 18 July 2018

OCA — Wed, 18 Jul 2018 08:32:44 GMT

←Older revision		Revision as of 08:32, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xynAS9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [http://pdbe.org/3wub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [http://www.ebi.ac.uk/pdbsum/3wub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [http://pdbe.org/3wub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [http://www.ebi.ac.uk/pdbsum/3wub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chainia sp]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Chen, C C]]		[[Category: Chen, C C]]

OCA at 12:13, 11 August 2016

OCA — Thu, 11 Aug 2016 12:13:45 GMT

←Older revision		Revision as of 12:13, 11 August 2016
Line 1:		Line 1:
		+
	==The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9==		==The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9==
	<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [http://www.ebi.ac.uk/pdbsum/3wub PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [http://pdbe.org/3wub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [http://www.ebi.ac.uk/pdbsum/3wub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wub ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3wub" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:01, 25 January 2015

OCA — Sun, 25 Jan 2015 10:01:46 GMT

←Older revision		Revision as of 10:01, 25 January 2015
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
-	[[Category: Chen, C C.]]	+	[[Category: Chen, C C]]
-	[[Category: Gao, J.]]	+	[[Category: Gao, J]]
-	[[Category: Guo, R T.]]	+	[[Category: Guo, R T]]
-	[[Category: Han, X.]]	+	[[Category: Han, X]]
-	[[Category: Huang, C H.]]	+	[[Category: Huang, C H]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Lv, P.]]	+	[[Category: Lv, P]]
-	[[Category: Peng, W.]]	+	[[Category: Peng, W]]
-	[[Category: Yang, Y Y.]]	+	[[Category: Yang, Y Y]]
-	[[Category: Zheng, Y.]]	+	[[Category: Zheng, Y]]
	[[Category: 4-xylanase]]		[[Category: 4-xylanase]]
	[[Category: Beta-1]]		[[Category: Beta-1]]

OCA at 11:13, 29 October 2014

OCA — Wed, 29 Oct 2014 11:13:48 GMT

←Older revision		Revision as of 11:13, 29 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9==
		+	<StructureSection load='3wub' size='340' side='right' caption='[[3wub]], [[Resolution\|resolution]] 2.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3wub]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WUB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3wue\|3wue]], [[3wuf\|3wuf]], [[3wug\|3wug]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wub OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wub RCSB], [http://www.ebi.ac.uk/pdbsum/3wub PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The glycoside hydrolase 10 (GH10) xylanase from Streptomyces sp. 9 (XynAS9) can operate in a broad range of pH and temperature, and thus is a potential candidate for commercial applications. Recently, we engineered XynAS9 via mutating several residues in accordance with the consensus sequences of GH10 thermophilic xylanases in an attempt to improve the enzyme thermostability and thermotolerance. The most promising effects were observed in the double mutant V81P/G82E. In order to investigate the molecular mechanism of the improved thermal profile of XynAS9, complex crystal structures of the wild type (WT) and mutant (MT) enzyme were solved at 1.88-2.05A resolution. The structures reveal a classical GH10 (beta/alpha)8 TIM-barrel fold. In MT XynAS9, E82 forms several interactions to its neighboring residues, which might aid in stabilizing the local structure. Furthermore, the MT structure showed lower B factors for individual residues compared to the WT structure, reflecting the increased MT protein rigidity. Analyses of the XynAS9 structures also delineate the detailed enzyme-substrate interaction network. More importantly, possible explanations for the enhanced thermal profiles of MT XynAS9 are proposed, which may be a useful strategy for enzyme engineering in the future.

-	~~The entry 3wub is ON HOLD until Paper Publication~~	+	Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability.,Chen CC, Luo H, Han X, Lv P, Ko TP, Peng W, Huang CH, Wang K, Gao J, Zheng Y, Yang Y, Zhang J, Yao B, Guo RT J Biotechnol. 2014 Sep 3;189C:175-182. doi: 10.1016/j.jbiotec.2014.08.030. PMID:25193708<ref>PMID:25193708</ref>

-	~~Authors~~: Chen, C.C., ~~Han, X., Lv, P~~., ~~Ko,~~ T.~~P., Peng~~, W., Huang, C.H., ~~Zheng, Y~~., ~~Gao, J~~., Yang, Y.Y., ~~Guo, R.T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~The wild type crystal structure of b~~-1~~,4-Xylanase (XynAS9) from Streptomyces sp. 9~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Chen, C C.]]
		+	[[Category: Gao, J.]]
		+	[[Category: Guo, R T.]]
		+	[[Category: Han, X.]]
		+	[[Category: Huang, C H.]]
		+	[[Category: Ko, T P.]]
		+	[[Category: Lv, P.]]
		+	[[Category: Peng, W.]]
		+	[[Category: Yang, Y Y.]]
		+	[[Category: Zheng, Y.]]
		+	[[Category: 4-xylanase]]
		+	[[Category: Beta-1]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein engineering]]
		+	[[Category: Protein rigidity]]
		+	[[Category: Thermozyme]]

OCA at 07:34, 24 September 2014

OCA — Wed, 24 Sep 2014 07:34:57 GMT

←Older revision		Revision as of 07:34, 24 September 2014
Line 5:		Line 5:
	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.		Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

-	Description: ~~Wild~~ type crystal structure of ~~a glycoside hydrolase~~	+	Description: The wild type crystal structure of b-1,4-Xylanase (XynAS9) from Streptomyces sp. 9

OCA at 05:32, 25 June 2014

OCA — Wed, 25 Jun 2014 05:32:32 GMT

←Older revision		Revision as of 05:32, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3wub is ON HOLD	+	The entry 3wub is ON HOLD until Paper Publication

	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.		Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

	Description: Wild type crystal structure of a glycoside hydrolase		Description: Wild type crystal structure of a glycoside hydrolase

OCA at 07:17, 7 May 2014

OCA — Wed, 07 May 2014 07:17:10 GMT

←Older revision		Revision as of 07:17, 7 May 2014
Line 3:		Line 3:
	The entry 3wub is ON HOLD		The entry 3wub is ON HOLD

-	Authors: Chen, C.C., Han, X., Lv, P., Ko, T,P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.	+	Authors: Chen, C.C., Han, X., Lv, P., Ko, T.P., Peng, W., Huang, C.H., Zheng, Y., Gao, J., Yang, Y.Y., Guo, R.T.

-	Description: ~~wild~~ type crystal structure of a glycoside hydrolase	+	Description: Wild type crystal structure of a glycoside hydrolase

OCA: Protected "3wub" [edit=sysop:move=sysop]

OCA — Wed, 30 Apr 2014 05:43:06 GMT

Protected "3wub" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 30 April 2014